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Regenerative Biomaterials 2021年第1期8卷 92-101页

作者： Changtian Gong Shuo Fang Kezhou Xia Jingteng Chen Liangyu guo weichun guo Department of Orthopedics Renmin Hospital of Wuhan UniversitWuhan 430060China

Incorporating bioactive substances into synthetic bioceramic scaffolds is *** this work,oxygen-carboxymethyl chitosan(0-CMC),a natural biopolymer that is nontoxic,biodegradable and biocompatible,was introduced into magnesium potassium phosphate cement(K-struvite)to enhance its mechanical properties and *** study aimed to develop 0-CMC/magnesium potassium phosphate composite bone cement(OMPC),thereby combining the opti-mum bioactivity of O-CMC with the extraordinary self setting properties and mechanical intensity of the *** results indicated that O-CMC incorporation increased the compressive strength and setting time of K-struvite and decreased its porosity and pH ***,OMPC scaffolds remarkably improved the proliferation,adhesion and osteogenesis related differ-entiation of MC3T3-E1 ***,O-CMC introduced suitable physicochemical properties to K-struvite and enhanced its cytocompatibility for use in bone regeneration.

关键词： magnesium phosphate cement oxygen-carboxymethyl chitos an physic ochemic al properties cytocompatility

Expression of heat shock protein gp96 in osteosarcoma and its clinical significance

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Frontiers of Medicine 2008年第2期2卷 200-203页

作者： weichun guo Anmin CHEN Department of Orthopaedics Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430030China Department of Orthopaedics Renmin Hospital of Wuhan UniversityWuhan 430060China

This study aims to detect the expression of heat shock protein gp96(HSPgp96)in osteosarcoma and explore its role in anti-tumor *** of HSPgp96 in 45 cases of osteosarcoma,including 24 cases of the osteoblast type,21 cases of chondroblast type and 5 cases of normal bone tissue were detected by immuno-histochemistry(IHC).The expression rate of HSPgp96 was 100%(45/45)in osteosarcoma,compared with 20%(1/5)in normal bone *** average positive cell rates were 97.23%and 0.48%in osteosarcoma and normal bone tissue,respectively,and the difference between them was significant(P0.05).The expression degree of HSPgp96 in osteosarcoma had a clear correlation with the pathologic degree(P0.05).The expression of HSPgp96 is high in osteosarcoma and it may be a significant index for judgment of malignancy degree and an important tar-get site of anti-tumor immunity.

关键词： osteosarcoma heat shock protein gp96 immunohistochemistry

Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway

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Bioactive Materials 2023年第2期20卷 598-609页

作者： Ling Yu Tian Gao Wei Li Jian Yang Yinchu Liu Yanan Zhao Ping He Xuefeng Li weichun guo Zhengfu Fan Honglian Dai Department of Orthopedic Surgery I(Spine Surgery) Renmin Hospital of Wuhan UniversityWuhan430060China Department of Bone and Soft Tissue Tumor Key Laboratory of Carcinogenesis and Translational ResearchMinistry of EducationPeking University Cancer Hospital&InstituteBeijing100000China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Biomedical Materials and Engineering Research Center of Hubei ProvinceWuhan University of TechnologyWuhan430070China Department of Orthopedic Surgery Renmin Hospital of ShayangJingmen448200China

There is a continuing need for artificial bone substitutes for bone repair and reconstruction,Magnesium phosphate bone cement(MPC)has exceptional degradable properties and exhibits promising ***,its mechanical strength needs improved and its low osteo-inductive potential limits its therapeutic application in bone *** functionally modified MPC by using a polymeric carboxymethyl chitosan-sodium alginate(CMCS/SA)gel *** had the advantages of:improved compressive strength,ease of handling,and an optimized interface for bioactive bone *** new composites with 2%CMCS/SA showed the most favorable physicochemical properties,including mechanical strength,wash-out resistance,setting time,injectable time and heat ***,the composite promoted the attachment and proliferation of osteoblast *** was also found to induce osteogenic differentiation in vitro,as verified by expression of osteogenic *** terms of molecular mechanisms,data showed that new bone cement activated the Wnt pathway through inhibition of the phosphorylation ofβ-catenin,which is dependent on focal adhesion *** micro-computed tomography and histological analysis,we found that the MPC-CMCS/SA scaffolds,compared with MPC alone,showed increased bone regeneration in a rat calvarial defect ***,our study suggested that the novel composite had potential to help repair critical bone defects in clinical practice.

关键词： Critical bone defect Magnesium phosphate cement Carboxymethyl chitosan Sodium alginate Osteogenic differentiation

Nanofiber-induced hierarchically-porous magnesium phosphate bone cements accelerate bone regeneration by inhibiting Notch signaling

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Bioactive Materials 2024年第7期37卷 459-476页

作者： Jingteng Chen Ling Yu Tian Gao Xiangyang Dong Shiyu Li Yinchu Liu Jian Yang Kezhou Xia Yaru Yu Yingshuo Li Sen Wang ZhengFu Fan Hongbing Deng weichun guo Department of Spine Surgery Renmin Hospital of Wuhan UniversityWuhan430060China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Bone and Soft Tissue TumorPeking University Cancer Hospital and InstituteBeijing100142China Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and EnergySchool of Resource and Environmental ScienceWuhan UniversityWuhan430079China

Magnesium phosphate bone cements(MPC)have been recognized as a viable alternative for bone defect repair due to their high mechanical strength and ***,their poor porosity and permeability limit osteogenic cell ingrowth and vascularization,which is critical for bone *** the current study,we constructed a novel hierarchically-porous magnesium phosphate bone cement by incorporating extracellular matrix(ECM)-mimicking electrospun silk fibroin(SF)*** SF-embedded MPC(SM)exhibited a heterogeneous and hierarchical structure,which effectively facilitated the rapid infiltration of oxygen and nutrients as well as cell ***,the SF fibers improved the mechanical properties of MPC and neutralized the highly alkaline environment caused by excess magnesium *** marrow stem cells(BMSCs)adhered excellently on SM,as illustrated by formation of more ***8 assay showed that SM promoted early proliferation of *** study also verified that SM increased the expression of OPN,RUNX2 and BMP2,suggesting enhanced osteogenic differentiation of *** screened for osteogenesis-related pathways,including FAK signaing,Wnt signaling and Notch signaling,and found that SM aided in the process of bone regeneration by suppressing the Notch signaling pathway,proved by the downregulation of NICD1,Hes1 and *** addition,using a bone defect model of rat calvaria,the study revealed that SM exhibited enhanced osteogenesis,bone ingrowth and vascularization compared with MPC *** adverse effect was found after implantation of SM in ***,our novel SM exhibited promising prospects for the treatment of critical-sized bone defects.

关键词： Critical-sized bone defects Magnesium phosphate bone cement Silk fibroin nanofibers Bone regeneration Notch signaling pathway

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调控骨肉瘤及干细胞的相关信号通路

中国组织工程研究 2020年第13期24卷 2102-2107页

作者：陶海郭卫春武汉大学人民医院骨科

背景:骨肉瘤是儿童和青少年中最常见的原发性骨源性恶性肿瘤,过去30年骨肉瘤患者的预后几乎没有大的进步,迫切需要开发新的策略和创新疗法,以进一步提高骨肉瘤患者的生存率。目的:阐述骨肉瘤进展和转移的分子机制和信号传导网络的最新进... 详细信息

背景:骨肉瘤是儿童和青少年中最常见的原发性骨源性恶性肿瘤,过去30年骨肉瘤患者的预后几乎没有大的进步,迫切需要开发新的策略和创新疗法,以进一步提高骨肉瘤患者的生存率。目的:阐述骨肉瘤进展和转移的分子机制和信号传导网络的最新进展,为治疗骨肉瘤提供一定的理论基础。方法:以"骨肉瘤,信号通路,预后,转移,耐药,Notch,Wnt/β-catenin,Hedgehog,PI3K/Akt/mTOR,BMPs"为中文检索词,以"osteosarcoma,signaling pathway,prognosis,metastasis,drug resistance,Notch,Wnt/β-catenin,Hedgehog,PI3K/Akt/mTOR,BMPs"为英文检索词,应用计算机在CNKI、Pub Med数据库检索1999年2月至2019年2月相关文献共586篇。通过阅读文献标题和摘要进行筛选,按排除标准剔除不相关的文献,最后共纳入40篇文献进行综述。结果与结论:信号通路在骨肉瘤的形成和增殖调控方面非常重要,研究发现Notch、Wnt/β-catenin、Hedgehog、PI3K/Akt/mTOR和BMP等信号通路在骨肉瘤及骨肉瘤干细胞中均有表达,对骨肉瘤的发生发展产生重要影响。

关键词：骨肉瘤信号通路转移耐药 Notch Wnt/β-catenin Hedgehog PI3K/Akt/mTOR BMPs

非特异性下腰痛:北美脊柱协会(NASS)循证医学指南解读

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中国修复重建外科杂志 2021年第10期35卷 1336-1340页

作者：施玉博郭卫春余铃武汉大学人民医院脊柱外科武汉430060

下腰痛是一种临床常见疾病。2020年北美脊柱协会(NASS)制定了非特异性下腰痛的循证医学指南,该指南主要围绕非特异性下腰痛的诊断、影像学检查、保守治疗、介入治疗和外科治疗五个方面进行论述。本文在充分研读指南基础上,对非特异性下... 详细信息

下腰痛是一种临床常见疾病。2020年北美脊柱协会(NASS)制定了非特异性下腰痛的循证医学指南,该指南主要围绕非特异性下腰痛的诊断、影像学检查、保守治疗、介入治疗和外科治疗五个方面进行论述。本文在充分研读指南基础上,对非特异性下腰痛诊治展开论述,以供临床参考。

关键词：非特异性下腰痛诊断治疗指南

腰骶部不同切除范围后腰骶骨盆重建的生物力学研究

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中华实验外科杂志 2015年第7期32卷 1552-1555页

作者：马亮郭卫春武汉大学人民医院骨外科 430060

目的评价腰骶部不同切除范围后腰骶骨盆重建后生物力学,为临床选择提供依据.方法 12具成年雄羊新鲜腰3～骨盆标本,下述分组测试.（1）完整组：腰4、腰5、骶1椎弓根螺钉固定.（2）骶1钉组：切除腰6 ～骶1椎间盘,两侧关节突,行腰4、5、骶... 详细信息

目的评价腰骶部不同切除范围后腰骶骨盆重建后生物力学,为临床选择提供依据.方法 12具成年雄羊新鲜腰3～骨盆标本,下述分组测试.（1）完整组：腰4、腰5、骶1椎弓根螺钉固定.（2）骶1钉组：切除腰6 ～骶1椎间盘,两侧关节突,行腰4、5、骶1椎弓根钉棒系统固定.（3）髂骨单钉组：切除腰6下1/2的椎体,骶1上1/2的椎体,行腰4、5、髂骨单钉固定.（4）髂骨双钉组：在第3组基础上行髂骨双钉固定.（5）髂骨双钉＋钛笼组：在第4组基础上加椎间双钛笼支撑.施加0 ～250 N轴向压力载荷及-5 ～5 N·m轴向扭矩载荷,记录轴向屈服载荷,屈服应力,计算轴向压缩刚度与轴向扭转刚度.结果第1～4组轴向屈服载荷为（203.1±8.0）、（188.9±7.5）、（165.7±7.2）、（239.2±9.8）N;屈服应力为（288.9±9.2）、（278.7±8.9）、（241.3±9.1）、（340.7±8.4） mPa,第5组没有发生明显的屈服.各组的轴向压缩刚度分别为（152.33±8.15）、（123.21±7.68）、（112.67±9.13）、（172.62±8.02）、（194.58±7.19） N·mm,轴向扭转刚度分别为（1.43±0.12）、（1.02±0.12）、（0.98±0.11）、（1.73±0.10）、（1.96±0.09） N·m/°.轴向压缩刚度及扭转刚度髂骨双钉＋钛笼组＞双钉组＞完整组,骶1钉组与髂骨单钉组稳定性均小于完整组,差异有统计学意义（P＜0.05）.骶1钉组与髂骨单钉组比较差异无统计学意义（P＞0.05）.结论在腰骶部病损范围较大时,应用髂骨双钉＋椎体间钛笼支撑可有效的恢复腰骶部的稳定性.

关键词：腰骶部脊柱骨盆重建生物力学髂骨双钉

单排技术与线桥技术修复兔全层肩袖损伤的比较

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中华创伤骨科杂志 2015年第4期17卷 331-336页

作者：费文勇郭卫春张文武汉大学人民医院骨科 430060 苏州大学附属第一医院骨科研究所

目的比较单排技术与线桥技术修复兔全层肩袖损伤的治疗效果. 方法取骨骼成熟的雄性新西兰大白兔76只,体质量为2.5～3.0 kg,平均2.8 kg,均为12个月龄.将兔冈上肌肌腱在足印区处切断制作兔全层肩袖模型,并随机分成2组（n=38）：采用单... 详细信息

目的比较单排技术与线桥技术修复兔全层肩袖损伤的治疗效果. 方法取骨骼成熟的雄性新西兰大白兔76只,体质量为2.5～3.0 kg,平均2.8 kg,均为12个月龄.将兔冈上肌肌腱在足印区处切断制作兔全层肩袖模型,并随机分成2组（n=38）：采用单排技术修复肩袖损伤（单排组）和线桥技术修复肩袖损伤（线桥组）,手术肩均为右侧肩关节,左肩关节作对照组不予任何处理.术后第2、4、8周取材,显微镜下观察、修补肩袖的愈合情况,并在术后第8周进行生物力学实验,分析2种方法的优、缺点. 结果线桥组在术后第2、4、8周时,修复肩袖的前1/3区域与中1/3区域腱-骨联合部比较,在软骨生长、胶原纤维形态方面均无明显差异;而单排组修复肩袖的中1/3区域腱-骨联合部软骨生长、胶原纤维形态均较前1/3区域成熟,差异明显.在术后第8周,修复冈上肌的腱-骨界面最大失效负荷线桥组[（134.59±17.69） N]明显大于单排组[（72.23±12.08） N],差异有统计学意义（P＜0.05）,但线桥组仍小于正常对照组[（192.61±9.42） N],差异有统计学意义（P＜0.05）.术后第8周时单排组2只兔出现肩袖再撕裂,而线桥组无一只兔出现失败. 结论线桥技术修复肩袖是一种可行、可靠的修复方法,较单排修复技术具有更优的愈合效果.

关键词：肩关节修复外科手术兔肩袖损伤

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黄芩素抑制143b骨肉瘤细胞增殖及其机制

武汉大学学报（医学版） 2021年第6期42卷 924-928页

作者：梅红军刘洋郭卫春武汉市第五医院骨科湖北武汉430050 武汉大学人民医院骨科湖北武汉430060

目的:研究不同浓度黄芩素(0,25,50,100μmol/L)对143b骨肉瘤细胞增殖的抑制作用及其作用机制。方法:采用CCK-8实验观察不同浓度黄芩素(0,25,50,100μmol/L)处理143b骨肉瘤细胞对细胞增殖的抑制作用;流式细胞仪分析黄芩素对143b细胞周期... 详细信息

目的:研究不同浓度黄芩素(0,25,50,100μmol/L)对143b骨肉瘤细胞增殖的抑制作用及其作用机制。方法:采用CCK-8实验观察不同浓度黄芩素(0,25,50,100μmol/L)处理143b骨肉瘤细胞对细胞增殖的抑制作用;流式细胞仪分析黄芩素对143b细胞周期分布和凋亡率的影响;Western Blot分析黄芩素对Bax、Bcl-2、Hes1和NICD1蛋白水平的影响。结果:黄芩素能时间和浓度依赖性地抑制143b细胞的增殖,诱导143b细胞周期阻滞于S期并促进骨肉瘤细胞凋亡(P<0.05)。黄芩素处理后,143b细胞中促凋亡蛋白Bax蛋白水平显著升高,抑凋亡蛋白Bcl-2蛋白水平显著降低(P<0.05);同时,细胞中Hes1和NICD1蛋白水平也显著降低(P<0.05)。结论:黄芩素能诱导143b细胞周期阻滞并能促进细胞凋亡发挥对143b细胞增殖的抑制作用,可能与抑制Notch信号通路活性相关。

关键词：黄芩素骨肉瘤 Notch信号通路细胞凋亡

Notch信号影响耐阿霉素骨肉瘤细胞干细胞特性

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武汉大学学报（医学版） 2021年第4期42卷 630-633页

作者：梅红军刘洋郭卫春武汉市第五医院骨科湖北武汉430050 武汉大学人民医院骨科湖北武汉430060

目的:探讨耐阿霉素骨肉瘤细胞干细胞特性的改变,并探究Jagged1/Notch1信号通路在其中所起的作用。方法:构建耐阿霉素骨肉瘤细胞,CCK-8实验检测细胞耐药性;Western Blot检测骨肉瘤细胞中干细胞相关基因(Oct4、Sox2、NANOG)蛋白表达水平,... 详细信息

目的:探讨耐阿霉素骨肉瘤细胞干细胞特性的改变,并探究Jagged1/Notch1信号通路在其中所起的作用。方法:构建耐阿霉素骨肉瘤细胞,CCK-8实验检测细胞耐药性;Western Blot检测骨肉瘤细胞中干细胞相关基因(Oct4、Sox2、NANOG)蛋白表达水平,及Jagged1、NICD1及Notch信号通路下游靶基因Hes1等蛋白表达水平;采用悬浮克隆球实验检测细胞成球能力。结果:耐阿霉素骨肉瘤细胞干细胞相关基因(Oct4、Sox2、NANOG)蛋白表达水平上调,悬浮克隆球形成能力更强,Jagged1/Notch1信号上调;使用γ-分泌酶抑制剂DAPT下调耐药骨肉瘤细胞中Jagged1/Notch1信号通路可以导致耐药骨肉瘤细胞悬浮克隆球形成能力下降,干细胞相关基因表达下调,抑制骨肉瘤细胞干细胞特性。结论:耐阿霉素骨肉瘤细胞干细胞特性增强,可能与Jagged1/Notch1信号激活有关。

关键词：骨肉瘤阿霉素 Jagged1/Notch1信号通路干细胞特性