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一例猪源性金黄色葡萄球菌的分离鉴定与药物敏感性试验

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中国农学通报 2024年第23期40卷 148-152页

作者：张鑫赵东辉李杰贾伟娟刘志林贾泽林崔佳宇王学理内蒙古民族大学动物科技学院内蒙古通辽028000 通辽市动物检疫技术服务中心内蒙古通辽028000 通辽市库伦旗动物疫病预防控制中心内蒙古通辽028000 通辽市农牧科学研究所内蒙古通辽028000

本研究旨在鉴定和分析锦州某猪场病死猪脏器中分离出的一株病原菌,以期为该细菌病的临床诊治提供科学依据。通过LB琼脂培养进行革兰氏染色,BP特殊培养基培养、药物敏感性试验、16S rDNA测序及小鼠致病性试验,并制作病理组织切片进行观... 详细信息

本研究旨在鉴定和分析锦州某猪场病死猪脏器中分离出的一株病原菌,以期为该细菌病的临床诊治提供科学依据。通过LB琼脂培养进行革兰氏染色,BP特殊培养基培养、药物敏感性试验、16S rDNA测序及小鼠致病性试验,并制作病理组织切片进行观察。由革兰氏染色镜检结果可知该菌为革兰氏阳性球菌;BP培养皿可见黑色菌落生长及菌落周边透明圈;药敏试验表明该菌对恩诺沙星、氧氟沙星、阿米卡星和卡那霉素非常敏感;经16S rDNA测序,该菌和金黄色葡萄球菌16S rDNA片段相似性高;镜检观察病理切片,可见各组织脏器有明显病理损害。该猪场致病菌经分离鉴定为金黄色葡萄球菌,对部分抗生素产生耐药,小鼠攻毒试验具有一定的致病性。

关键词：猪金黄色葡萄球菌分离鉴定耐药性药物敏感性试验 16S rDNA测序

Different Starting Dominant Strain of Seasonal Influenza in China and Other Neighboring Asian Countries in 2019−2020 Winter Season

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China CDC weekly 2020年第4期2卷 57-60页

作者： Jing Yang weijuan Huang Yanhui Cheng Tao Chen Minju Tan Qian Guo Hejiang Wei Fei Zhu Tomoko Kuwahara Xiyan Li Xiang zhao Hideki Hasegawa George F.Gao Dayan Wang National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionWHO Collaborating Center for Reference and Research on InfluenzaKey Laboratory for Medical VirologyNational Health CommissionBeijingChina Laboratory of Influenza Virus Surveillance Influenza Virus Research CenterNational Institute of Infectious DiseasesWHO Collaborating Center for Reference and Research on InfluenzaTokyoJapan Chinese Center for Disease Control and Prevention BeijingChina.

Introduction:Seasonal influenza is a prevalent and highly contagious acute respiratory disease that causes a major global disease *** strains of influenza viruses also have the potential to cause an influenza *** to the high variability and the consequent uncertainty about virus strains,the prevention and control of influenza are faced with many *** is considered a key strategy to lead to the prevention and control of influenza,and influenza is one of first infectious diseases to be monitored ***:The Chinese National Influenza Surveillance Network conducts routine influenza surveillance and includes 410 network laboratories and 554 sentinel hospitals based on the requirements of National Influenza Surveillance *** and other countries submit epidemiological and virological data to the WHO FluMart database,which can be viewed ***:Seasonal influenza H3N2 virus was the dominant subtype in China when entering the 2019–2020 winter influenza season,while neighboring countries such as Japan and Republic of Korea reported the dominance of H1N1pdm09 and Mongolia and Russia reported a higher proportion of type B virus than type A *** and Implications for Public Health Practice:Asian countries entered the 2019–2020 winter influenza season with different type/subtypes of influenza virus dominant in different *** surveillance needs to be strengthened to closely monitor changes in the antigenicity and genetic characteristics of emerging viruses.

关键词： prevention strengthened submit

Novel and potent inhibitors targeting DHODH are broad-spectrum antivirals against RNA viruses including newly-emerged coronavirus SARS-CoV-2

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Protein & Cell 2020年第10期11卷 723-739页

作者： Rui Xiong Leike Zhang Shiliang Li Yuan Sun Minyi Ding Yong Wang Yongliang zhao Yan Wu weijuan Shang Xiaming Jiang Jiwei Shan Zihao Shen Yi Tong Liuxin Xu Yu Chen Yingle Liu Gang Zou Dimitri Lavillete Zhenjiang zhao Rui Wang Lili Zhu Gengfu Xiao Ke Lan Honglin Li Ke Xu State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China Shanghai Key Laboratory of New Drug Design State Key Laboratory of Bioreactor EngineeringSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China CAS Key Laboratory of Molecular Virology and Immunology Institut Pasteur of ShanghaiUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China

Emerging and re-emerging RNA viruses occasionally cause epidemics and pandemics worldwide,such as the on-going outbreak of the novel coronavirus ***,we identified two potent inhibitors of human DHODH,S312 and S416,with favorable drug-likeness and pharmacokinetic profiles,which all showed broad-spectrum antiviral effects against various RNA viruses,including influenza A virus,Zika virus,Ebola virus,and particularly against ***,S416 is reported to be the most potent inhibitor so far with an EC5o of 17 nmol/L and an SI value of 10,505.88 in infec-ted *** results are the first to validate that DHODH is an attractive host target through high antiviral efficacy in vivo and low virus replication in DHODH knock-out *** work demonstrates that both S312/S416 and old drugs(Leflunomide/Teriflunomide)with dual actions of antiviral and immuno-regulation may have clinical potentials to cure SARS-CoV-2 or other RNA viruses circulating worldwide,no matter such viruses are mutated or not.

关键词： de novo pyrimidine biosynthesis DHODH inhibitors SARS-CoV-2 influenza viruses virus replication immuno-regulation

Correction to: Novel and potent inhibitors targeting DHODH are broad-spectrum antivirals against RNA viruses including newly-emerged coronavirus SARS-CoV-2

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Protein & Cell 2021年第1期12卷 76-80页

Figure *** of novel and potent DHODHi and their anti-influenza A virus activities.(A)The discovery and design of S312 and *** detailed descriptions of the discovery workflow are in *** analysis of S312(B)and S416(C).Thermodynamic analysis of the binding of S312 and S416 to DHODH was carried out at 25 C on a MicroCal iTC200 *** analysis of the binding of S312 and S416 to DHODH was performed with a Biacore T200 instrument.

关键词： Figure discovery ODH

Progress of ISO Standards Development onAcupuncture Needles

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China Standardization 2015年第4期65卷 78-81页

作者：王芳赵宏岗卫娟 Acupuncture Standardization Center of the Acupuncture Institute of CACMS Acupuncture Hospital of CACMS

Based on the decision to set up a work group at the second plenary meeting of ISO/ TC 249 in Hague, the Netherlands from Nay 2 to 4, 2011, ISO/TC 249/WG 3 was created by ISO/CS on May 30, which would mainly develop standards on acupuncture needles. Registered as the convener of WG 3 in the ISO Global Directory by ISO/CS on July 5, Huang Longxiang, research fellow in the Institute of Acupuncture of China Academy of Chinese Medical Sciences （CACMS） chaired the first meeting of the work group from October 20 to 21.

关键词：国际标准化针灸工作组科学院研究员研究所 TC CS

Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq

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Molecular Plant 2021年第4期14卷 556-570页

作者： Mengjiao Chen weijuan Fan Feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling zhao Jianli Zheng Qi Zhang Jiaxin Chen Jie Qiu Xin Wei Ziru Chen Peng Zhang Dong Pei Jun Yang Xuehui Huang Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai Chenshan Botanical GardenShanghai 201602China State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Tai'an Academy of Agricultural Sciences Tai'an 271000ShandongChina National Genomics Data Center Bio-Med Big Data CenterCAS Key Laboratory of Computational BiologyCAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China CAS Center for Excellence of Molecular Plant Sciences Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200233China These authors contributed equally to this article.

Many important crops(e.g.,tuber,root,and tree crops)are *** these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid *** this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing *** applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic *** causative genes at these loci were detected based on functional *** together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient *** OutcrossSeq software and its instruction manual are available for downloading at ***/tool/***.

关键词： genotyping genetic mapping outcrossing polyploid

High-throughput screening identifies established drugs as SARS-CoV-2 PLpro inhibitors

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Protein & Cell 2021年第11期12卷 877-888页

作者： Yao zhao Xiaoyu Du Yinkai Duan Xiaoyan Pan Yifang Sun Tian You Lin Han Zhenming Jin weijuan Shang Jing Yu Hangtian Guo Qianying Liu Yan Wu Chao Peng Jun Wang Chenghao Zhu Xiuna Yang Kailin Yang Ying Lei Luke W.Guddat Wenqing Xu Gengfu Xiao Lei Sun Leike Zhang Zihe Rao Haitao Yang Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Laboratory of Structural Biology School of Life Sciences and School of MedicineTsinghua UniversityBeijing 100091China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China The Fifth People's Hospital of Shanghai Fudan University and Shanghai Key Laboratory of Medical EpigeneticsInstitutes of Biomedical SciencesFudan UniversityShanghai 200032China Zhangjiang Lab National Facility for Protein Science in ShanghaiShanghai Advanced Research InstituteChinese Academy of ScienceShanghai 201210China Taussig Cancer Center Cleveland ClinicClevelandOH 44195USA School of Chemistry and Molecular Biosciences The University of QueenslandBrisbaneQueensland 4072Australia

A new coronavirus(SARS-CoV-2)has been identified as the etiologic agent for the COVID-19 ***,effective treatment options remain very limited for this disease;therefore,there is an urgent need to identify new anti-COVID-19 *** this study,we screened over 6,000 compounds that included approved drugs,drug candidates in clinical trials,and pharmacologically active compounds to identify leads that target the SARS-CoV-2 papain-like protease(PLpro).Together with main protease(Mpro),PLpro is responsible for processing the viral replicase polyprotein into functional ***-fore,it is an attractive target for antiviral drug *** we discovered four compounds,YM155,cryptotanshinone,tanshinone I and GRL0617 that inhibit SARS-CoV-2 PLpro with IC50 values ranging from 1.39 to 5.63 pmol/*** compounds also exhibit strong antiviral activities in cell-based ***155,an anti-cancer drug candidate in clinical trials,has the most potent antiviral activity with an EC50 value of 170 nmol/*** addition,we have determined the crystal structures of this enzyme and its complex with YM155,revealing a unique binding ***155 simultaneously targets three"hot"spots on PLpro,including the substrate-binding pocket,the interferon stimulating gene product 15(ISG15)binding site and zinc finger *** results demonstrate the efficacy of this screening and repur-posing strategy,which has led to the discovery of new drug leads with clinical potential for COVID-19 treatments.

关键词： SARS-CoV-2 papain-like protease YM155 interferon stimulating gene product 15 drug repurposing

Comparative Antiviral Efficacy of Viral Protease Inhibitors against the Novel SARS-CoV-2 In Vitro

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Virologica Sinica 2020年第6期35卷 776-784页

作者： Leike Zhang Jia Liu Ruiyuan Cao Mingyue Xu Yan Wu weijuan Shang Xi Wang Huanyu Zhang Xiaming Jiang Yuan Sun Hengrui Hu Yufeng Li Gang Zou Min Zhang Lei zhao Wei Li Xiaojia Guo Xiaomei Zhuang Xing-Lou Yang Zheng-Li Shi Fei Deng Zhihong Hu Gengfu Xiao Manli Wang Wu Zhong State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China National Engineering Research Center for the Emergency Drug Beijing Institute of Pharmacology and ToxicologyBeijing 100850China CAS Key Laboratory of Molecular Virology and Immunology Institute Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China

The recent outbreak of novel coronavirus pneumonia(COVID-19)caused by a new coronavirus has posed a great threat to public *** safe and effective antivirals is of urgent demand to cure the huge number of *** proteases are considered potential drug *** human immunodeficiency virus protease inhibitors(lopinavir/ritonavir)has been recommended in the global Solidarity Trial in March launched by World Health ***,there is currently no experimental evidence to support or against its clinical *** evaluated the antiviral efficacy of lopinavir/ritonavir along with other two viral protease inhibitors in vitro,and discussed the possible inhibitory mechanism in *** in vitro to in vivo extrapolation was carried out to assess whether lopinavir/ritonavir could be effective in *** the four tested compounds,lopinavir showed the best inhibitory effect against the novel coronavirus ***,further in vitro to in vivo extrapolation of pharmacokinetics suggested that lopinavir/ritonavir could not reach effective concentration under standard dosing regimen[marketed as Kaletraò,contained lopinavir/ritonavir(200 mg/50 mg)tablets,recommended dosage is 400 mg/10 mg(2 tablets)twice daily].This research concluded that lopinavir/ritonavir should be stopped for clinical use due to the huge gap between in vitro IC50 and free plasma ***,the structure–activity relationship analysis of the four inhibitors provided further information for de novel design of future viral protease inhibitors of SARS-CoV-2.

关键词： Respiratory pharmacology COVID-19 SARS-CoV-2 Protease inhibitor

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保持一类正规特征值的可加映射

吉林大学学报（理学版） 2020年第4期58卷 798-802页

作者：赵旭史维娟吉国兴陕西师范大学数学与信息科学学院西安710119

设B(X)是无限维复Banach空间上全体有界线性算子组成的代数.利用算子谱的性质研究B(X)上双边保持部分正规特征值可加满射的结构,证明该映射是B(X)上的同构或反同构.

关键词：正规特征值可加保持谱算子