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Heparanase-1 is downregulated in chemoradiotherapy orbital rhabdomyosarcoma and relates with tumor growth as well as angiogenesis

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 31-39页

作者： wei-qiang tang Yan Hei Jing Lin Department of Ophthalmology the Fourth Medical CentreChinese PLA General HospitalBeijing 100048China Department of Ophthalmology the Third Medical CentreChinese PLA General HospitalBeijing 100039China Department of Clinical Laboratory the Fourth Medical CentreChinese PLA General HospitalBeijing 100048China

AIM:To determine the role of heparanase-1(HPSE-1)in orbital rhabdomyosarcoma(RMS),and to investigate the feasibility of HPSE-1 targeted therapy for ***:Immunohistochemistry was performed to analyze HPSE-1 expression in 51 cases of orbital RMS patients(including 28 cases of embryonal RMS and 23 cases of alveolar RMS),among whom there were 27 treated and 24 untreated with preoperative *** vitro,studies were conducted to examine the effect of HPSE-1 silencing on RMS cell proliferation and tube formation of human umbilical vein endothelial cells(HUVECs).RD cells(an RMS cell line)and HUVECs were infected with HPSE-1 sh RNA lentivirus at a multiplicity of infection(MOI)of 10 and 30 ***-time PCR and Western blot were applied to detect the m RNA and protein expression levels of *** viability of treated or control RD cells was evaluated by cell counting kit-8(CCK-8)*** tube formation assay was used to evaluate the effect of HPSE-1 RNAi on the tube formation of ***:Immunohistochemistry showed that the expression rate of HPSE-1 protein was 92.9%in orbital embr yonal RMS and 91.3%in orbital alveolar *** from alveolar orbital RMS did not show relatively stronger staining than that from the embryonal orbital ***,despite the types of RMS,comparing the cases treated chemoradiotherapy with those untreated,we have observed that chemoradiotherapy resulted in weaker staining in patients’*** expression levels of HPSE-1 declined significantly in both the m RNA and protein levels in HPSE-1 sh RNA transfected RD *** CCK-8 assay showed that lentivirus-mediated HPSE-1 silencing resulted in significantly reduced RD cells viability in *** HPSE-1 expression also inhibited VEGF-induced tube formation of HUVECs in ***:HPSE-1 silencing may be a promising therapy for the inhibition of orbital RMS progression.

关键词： heparanase-1 rhabdomyosarcoma CCK-8 tube formation chemoradiotherapy

Primary Sjgren's syndrome related optic neuritis

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International Journal of Ophthalmology(English edition) 2013年第6期6卷 888-891页

作者： wei-qiang tang Shi-Hui wei Department of Ophthalmology the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army General Hospital of Chinese People's Liberation Army

AIM:To determine the clinical features,diagnosis and treatment of the primary Sjogren syndrome(SS)related optic ***:The clinical data of 8 patients(12 eyes)with primary SS related optic neuritis were analyzed ***:Eight of 128 consecutive patients with optic neuritis resulted from varied causes fulfilled the diagnostic criteria for the primary *** presented initially with the signs and symptoms of non-specific optic neuritis,and 5 patients presenting without dryness showed a chronic inflammation of submandibular gland or parotid gland,and lymphocyte infiltration was demonstrated by labial gland biopsy in 2 *** were serum positive titers for anti-Sjogren syndrome A(SSA)in 7 patients and anti-Sjogren syndrome B(SSB)in 8 ***-aquaporin-4(AQP4)antibody was negative in all the 8 *** glucocorticoids and immunosuppressive agent were administered,and visual acuity elevated in 8 eyes(66.7%),3 patients(37.5%)recurred in the ***:Primary SS related optic neuritis is less common and easily *** conventional therapies for optic neuritis could not control the recurrence.

关键词： optic neuritis primary Sjgren's syndrome anti-aquaporin-4

Integration of perioperative features and intragraft TCF7L2 expression to predict lipid metabolic disorder in liver transplant recipients

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Science China Life Sciences 2025年第1期 297-299页

作者： Junbin Zhou Rongli wei Guanghan Fan Zhengxing Lian Xuanyu Zhang Linsong tang Zhetuo Qi Haiyang Xie Shusen Zheng qiang wei Xiao Xu Department of Hepatobiliary and Pancreatic Surgery People's Hospital of LishuiAffiliated Lishui Hospital of Wenzhou Medical UniversityThe First Affiliated Hospital of Lishui University NHFPC Key Laboratory of Combined Multi-Organ Transplantation Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province Department of Hepatobiliary and Pancreatic SurgeryHangzhou First People's Hospital Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of Medicine Department of Urology The Second Affiliated HospitalZhejiang University School of Medicine Department of Hepatobiliary and Pancreatic Surgery Department of Liver TransplantationShulan (Hangzhou) Hospital Hangzhou Medical College Zhejiang University School of Medicine

Dear Editor, Liver transplantation(LT) is the only curative therapy for patients with endstage liver disease(He et al., 2023; Ling et al., 2022). Post-transplant hypertriglyceridemia(PTHT), which has an incidence of up to 32.8% in 6 months after LT, is one of the major post-transplant complications(Toshima et al., 2020).

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基于位移的输电塔地震易损性分析

钢结构 2016年第12期31卷 47-52页

作者：张伟周强唐可人广西大学土木建筑工程学院南宁530004 广西大学工程防灾与结构安全教育部重点实验室南宁530004

针对输电塔地震易损性分析中极限状态和震害等级划分较粗、难以准确预测各级震害等问题,以直线酒杯型输电塔为例,选取最大塔顶水平位移作为工程需求参数,采用非线性静力分析确定了对应输电塔发生轻微损坏、构件屈服、结构屈服和结构倒塌... 详细信息

针对输电塔地震易损性分析中极限状态和震害等级划分较粗、难以准确预测各级震害等问题,以直线酒杯型输电塔为例,选取最大塔顶水平位移作为工程需求参数,采用非线性静力分析确定了对应输电塔发生轻微损坏、构件屈服、结构屈服和结构倒塌等4个性能水平的极限状态,将输电塔震害划分为5个等级,进一步全面考虑风荷载、导地线荷载和自重荷载等,结合增量动力分析法系统提出了输电塔的地震易损性分析方法。

关键词：输电塔易损性分析位移极限状态震害等级

运动与吸烟对健康青壮年男性心率变异性的影响

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中国康复医学杂志 2017年第8期32卷 916-922页

作者：艾磊罗维朱晓梅汤强刘凌江苏省体育科学研究所南京体育学院南京210014

目的:探讨每周训练时长与吸烟两个潜在的自主神经功能影响因子对健康青壮年男性心率变异性(HRV)的交互影响,为改善青壮年男性人群自主神经功能、降低心血管疾病发病率提供相应参考。方法:本研究共选取了149例受试者,依据受试者吸烟指数... 详细信息

目的:探讨每周训练时长与吸烟两个潜在的自主神经功能影响因子对健康青壮年男性心率变异性(HRV)的交互影响,为改善青壮年男性人群自主神经功能、降低心血管疾病发病率提供相应参考。方法:本研究共选取了149例受试者,依据受试者吸烟指数的大小分为不吸烟组(N组,n=66例)、吸烟多组(M组,n=45例)以及吸烟少组(F组,n=38例),其中,N组、M组和F组依据每周训练时间的长短又都分为运动多和运动少两个亚组,分别为:不吸烟运动少组(NF组,n=28例)、不吸烟运动多组(NM组,n=38例)、吸烟多运动多组(MM组,n=22例)、吸烟多运动少组(MF组,n=23例)、吸烟少运动多组(FM组,n=18例)和吸烟少运动少组(FF组,n=20例)。HRV测试包括时域指标有SDNN、RMSSD、SDSD以及PNN50等,频域指标有TP、HF、LF、HFnu、LFnu、LF/HF以及VLF等。结果:(1)不吸烟时,NM和NF组心率变异性的时域及频域各指标相差不大(P>0.05);吸烟相对较少时,FM组的TP、HF和LF等指标的值均大于FF组对应指标的值(P<0.05);吸烟相对较多时,MM组的LF/HF值大于MF组的LF/HF值(P<0.05)。(2)运动相对较少时,NF组的LF值大于MF组的LF值(P<0.05);运动相对较多时,NM组和FM组各时域及频域指标中除LF/HF和LFnu以外的其他各项指标的均值均大于MM组对应各指标的均值,其中,FM组的RMSSD和SDSD等的值与MM组对应两指标值的差异具有显著性意义(P<0.05)。结论:(1)吸烟和缺乏规律性的健身活动(每周训练时间少于300min)都是影响心率变异性的风险因素。(2)吸烟指数少于200者,规律性的健身活动(每周总时长超过300min)对于心率变异性存在明显的改善作用。(3)吸烟指数大于200者,规律性的健身活动(每周总时长超过300min)未能干预吸烟对心率变异性形成的不良影响,必须戒烟。(4)运动强度作为影响心率变异性的一个重要因子,建议后续研究运动对于心率变异性影响时应作重点具体化考量。(5)吸烟引起心率变异性的变化是个急性效应,建议后续研究吸烟对于心率变异性的影响可以将每日吸烟支数作为影响因子。

关键词：吸烟运动心率变异性自主神经迷走神经

乙酰肝素酶抑制剂对体外培养视网膜色素上皮细胞增殖的影响

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国际眼科杂志 2012年第8期12卷 1463-1466页

作者：唐维强张岩李晓艳解放军总医院第一附属医院眼科中国北京市100048

目的:探讨乙酰肝素酶(heparanase-1,HPA-1)抑制剂应用对体外培养的视网膜色素上皮(retinal pigment epithelial,RPE)细胞增殖的影响。方法:采用DMEM培养基(Dulbecco's modified eagles medium,DMEM)体外培养人RPE细胞,选择第5代细胞用于实验。倒置显微镜下直接观察不同质量浓度硫代磷酸甘露醇戊糖(phosphomannopentaose sulfate,PI-88)对人RPE细胞体外生长的干预效果,应用四甲基偶氮唑盐[3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny1 tetrazolium bromide,MTT]比色法检测RPE细胞A570值;免疫组织化学方法检测人RPE细胞角蛋白和HPA-1表达。结果:体外增生的RPE细胞胞浆和细胞核HPA-1呈强阳性表达,以细胞浆内表达为主,PI-88干预后细胞浆表达减弱。PI-88对体外RPE细胞的增生有明显的时效和量效抑制关系,药物干预72h细胞A570值随质量浓度不同表现明显下降趋势(P<0.05);而同一质量浓度,药物质量浓度达到100mg/L及以上时,48～72h才表现A570值减小的趋势。结论:HPA-1抑制剂可抑制体外培养RPE细胞的增生,并呈现剂量和时间依赖关系。

关键词：硫代磷酸甘露醇戊糖乙酰肝素酶视网膜色素上皮

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金属主链高分子的量子化学计算

高分子学报 2023年第4期54卷 413-417页

作者：张义锋曾凯雯杨依蓓唐伟强彭慧胜聚合物分子工程国家重点实验室、复旦大学高分子科学系、先进材料实验室上海200438 华东理工大学化学工程学院、化学工程国家重点实验室上海200237

金属主链高分子是一类完全以金属原子作为主链的新材料,在光电、传感、电化学储能等领域都具有重要的应用前景.但是,如何获得具有较高分子量的金属主链高分子,仍然是一个挑战.本文通过密度泛函理论计算方法,对镍金属主链高分子进行了结... 详细信息

金属主链高分子是一类完全以金属原子作为主链的新材料,在光电、传感、电化学储能等领域都具有重要的应用前景.但是,如何获得具有较高分子量的金属主链高分子,仍然是一个挑战.本文通过密度泛函理论计算方法,对镍金属主链高分子进行了结构优化和稳定性分析,发现随着聚合物分子量的增加,镍金属主链高分子的单点能逐渐降低,说明高分子量的金属主链高分子具有稳定的分子构型.但是,随着分子量的进一步提高,计算量显著增加,密度泛函理论计算方法收敛困难.为此,本文进一步通过Hartree-Fock计算方法对该系列镍金属主链高分子进行了分子构型优化,得到了与密度泛函理论计算结果相似的规律,并且发现当分子量超过1×10^(4)时,金属主链高分子仍然具有较高的结构稳定性.此外,这种电子离域在整个金属主链上的独特金属-金属的相互作用有利于实现电荷的高效传输;以金属原子为重复单元的分子结构,有望实现金属和高分子材料性能的有效结合,可以获得较高的热失重温度和熵弹性.

关键词：金属主链镍原子高分子理论计算

应用彩色多普勒血流成像技术对后部缺血性视神经病变的研究

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国际眼科杂志 2009年第8期9卷 1516-1518页

作者：皮裕琍张晶唐维强周昀董莹中国人民解放军总医院第一附属医院眼科中国北京市100048 中国人民解放军总医院第一附属医院超声科中国北京市100048

目的:探讨后部缺血性视神经病变(posterior ischenic optic neuropathy,PION)患者颈动脉及眼血流动力学的改变。方法:应用彩色多普勒血流成像技术(color Doppler flow imaging,CDFI)观察14例19眼PION患者,检测颈总动脉和颈内动脉影像学... 详细信息

目的:探讨后部缺血性视神经病变(posterior ischenic optic neuropathy,PION)患者颈动脉及眼血流动力学的改变。方法:应用彩色多普勒血流成像技术(color Doppler flow imaging,CDFI)观察14例19眼PION患者,检测颈总动脉和颈内动脉影像学特征、睫状后动脉(posterior ciliary arteries,PCAs)和视网膜中央动脉(central retinal artery,CRA)血流动力学变化,与患者未受累一侧眼进行比较。结果:14例PION患者中12例(86%)表现为双侧颈总动脉和颈内动脉内膜粗糙、增厚,9例(64%)探查到血管壁斑块回声,硬斑5例,混合斑5例,软斑3例;PCAs的收缩期峰值速度(peaksy stolic velocity,PSV)为27.60±16.7cm/s,阻力指数(resistance index,RI)为0.84±0.038,与对照眼比较PSV无显著降低,RI明显增高(t=2.116,P<0.05);CRA的PSV为11.51±3.47cm/s,RI为0.75±0.036,与对照眼比较PSV无差异,RI增高非常明显(t=2.862,P<0.01)。结论:PION的发生主要与颈动脉粥样硬化导致的颈动脉系统血流变化有关,CDFI对明确PION诊断有重要的应用价值。

关键词：后部缺血性视神经病变颈动脉眼血流彩色多普勒血流成像技术

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing Zhang Xue Fang Hongmei Luo Chuanhai Fu Zhiyi wei Ai-Hui tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1