Heparanase-1 is downregulated in chemoradiotherapy orbital rhabdomyosarcoma and relates with tumor growth as well as angiogenesis
作者机构:Department of Ophthalmologythe Fourth Medical CentreChinese PLA General HospitalBeijing 100048China Department of Ophthalmologythe Third Medical CentreChinese PLA General HospitalBeijing 100039China Department of Clinical Laboratorythe Fourth Medical CentreChinese PLA General HospitalBeijing 100048China
出 版 物:《International Journal of Ophthalmology(English edition)》 (国际眼科杂志(英文版))
年 卷 期:2022年第15卷第1期
页 面:31-39页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题:heparanase-1 rhabdomyosarcoma CCK-8 tube formation chemoradiotherapy
摘 要:AIM:To determine the role of heparanase-1(HPSE-1)in orbital rhabdomyosarcoma(RMS),and to investigate the feasibility of HPSE-1 targeted therapy for ***:Immunohistochemistry was performed to analyze HPSE-1 expression in 51 cases of orbital RMS patients(including 28 cases of embryonal RMS and 23 cases of alveolar RMS),among whom there were 27 treated and 24 untreated with preoperative *** vitro,studies were conducted to examine the effect of HPSE-1 silencing on RMS cell proliferation and tube formation of human umbilical vein endothelial cells(HUVECs).RD cells(an RMS cell line)and HUVECs were infected with HPSE-1 sh RNA lentivirus at a multiplicity of infection(MOI)of 10 and 30 ***-time PCR and Western blot were applied to detect the m RNA and protein expression levels of *** viability of treated or control RD cells was evaluated by cell counting kit-8(CCK-8)*** tube formation assay was used to evaluate the effect of HPSE-1 RNAi on the tube formation of ***:Immunohistochemistry showed that the expression rate of HPSE-1 protein was 92.9%in orbital embr yonal RMS and 91.3%in orbital alveolar *** from alveolar orbital RMS did not show relatively stronger staining than that from the embryonal orbital ***,despite the types of RMS,comparing the cases treated chemoradiotherapy with those untreated,we have observed that chemoradiotherapy resulted in weaker staining in patients’*** expression levels of HPSE-1 declined significantly in both the m RNA and protein levels in HPSE-1 sh RNA transfected RD *** CCK-8 assay showed that lentivirus-mediated HPSE-1 silencing resulted in significantly reduced RD cells viability in *** HPSE-1 expression also inhibited VEGF-induced tube formation of HUVECs in ***:HPSE-1 silencing may be a promising therapy for the inhibition of orbital RMS progression.