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Diagnosis of cytomegalovirus encephalitis using metagenomic nextgeneration sequencing of blood and cerebrospinal fluid:A case report

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World Journal of Clinical Cases 2022年第14期10卷 4601-4607页

作者： Chang-Qing Xu Xia-Ling Chen Dong-Sheng Zhang Jia-wei Wang Hong Yuan wei-Fan Chen Han Xia Zhong-Yin Zhang Fu-hua Peng Department of Neurology Dongguan Kanghua HospitalDongguan 523080Guangdong ProvinceChina Department of Scientific Affairs Hugobiotech Co.Ltd.Beijing 100176China Department of Neurology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina

BACKGROUND Cytomegalovirus(CMV)infections in the population are mostly subclinical,inapparent,or ***,it is rare in brain *** reported CMV encephalitis cases were patients with *** diagnosis and detection rate of CMV encephalitis in patients with normal immune function needs to be further *** SUMMARY An 86-year-old male was admitted due to a sudden onset of unconsciousness for 3 *** patient developed status epilepticus and was relieved after antiepileptic *** was considered due to the high signals of diffusionweighted imaging sequences in the right central region by magnetic resonance *** next-generation sequencing(mNGS)of blood and cerebrospinal fluid revealed CMV,with unique reads number being 614 and 1,*** quantitative PCR results showed CMV positive in blood samples and negative in cerebrospinal fluid *** patient was finally diagnosed as CMV encephalitis with status *** the antiviral,hormonal,andγ-globulin pulse therapy,the patient’s condition improved,and he was finally *** mNGS could be a reliable approach for the diagnosis of CMV encephalitis,with high efficiency,sensitivity,and specificity.

关键词： Cytomegalovirus encephalitis Metagenomic next-generation sequencing Quantitative real-time PCR Diagnosis Case report

Chinese Medicines in Diabetic Retinopathy Therapies

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Chinese Journal of Integrative Medicine 2019年第4期25卷 316-320页

作者： song wei ZHU Yong-wei Department of Ophthalmology Jiaxing Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical UniversityZhejiang Province(314000)China

Diabetic retinopathy(DR),a chronic microvascular retinal disorder leading to retinal nonperfusion and ischemia,is one of the leading causes of blindness among individuals of working ***?ammation and neovascularization play important roles in the development of DR,especially proliferative DR(PDR).Therapies with Chinese medicines(CMs) that improve microcirculation complementary to conventional treatments increase the chances of delaying PDR development and improving visual acuity in diabetes *** review aimed to introduce promising CMs targeting DR patients in clinical practice,together with their underlying molecular mechanisms.

关键词： diabetic retinopathy Chinese medicine inflammation neovascularization

NLRP3炎症小体在认知功能障碍相关疾病防治中的作用及中药干预研究进展

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中国中药杂志 2024年第4期49卷 902-911页

作者：王彩红魏晓涛赵永强王东红薛建军宋子晴汤峰刘荣鑫王春爱甘肃中医药大学第一临床医学院甘肃兰州730000 甘肃省中医院泌尿外科甘肃兰州730000 甘肃省中医院麻醉科甘肃兰州730000 甘肃省中西医结合麻醉临床研究中心甘肃兰州730000 甘肃省中医药研究院中西医结合麻醉与循证医学研究所甘肃兰州730000

阿尔茨海默病(Alzheimer′s disease,AD)、血管性痴呆(vascular dementia,VD)及创伤性脑损伤(traumatic brain injury,TBI)作为认知功能障碍疾病中较常见的疾病,具有高致残率、高致死率的特点,给个体家庭带来了沉重的负担。AD、VD、TBI... 详细信息

阿尔茨海默病(Alzheimer′s disease,AD)、血管性痴呆(vascular dementia,VD)及创伤性脑损伤(traumatic brain injury,TBI)作为认知功能障碍疾病中较常见的疾病,具有高致残率、高致死率的特点,给个体家庭带来了沉重的负担。AD、VD、TBI虽具体发病机制不同,但其发病均与核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3)息息相关。NLRP3炎症小体通过参与神经炎症反应、介导小胶质细胞极化、调控减少β淀粉样蛋白(amyloidβ-protein,Aβ)沉积、神经纤维缠结(neurofibrillary tangles,NFTs)形成、调控细胞自噬、维持大脑内稳态、维持突触稳态等方式,参与AD、VD、TBI发生发展。同时既往研究证实,中药可通过干预NLRP3炎症小体发挥减轻神经炎症、促进小胶质细胞向M2极化、减少Aβ沉积和NFTs形成、调控细胞自噬、维持大脑内稳态的作用,起到防治认知功能障碍相关疾病的同时减轻患者心理及经济压力、提高患者生活质量的作用。故该文拟通过阐述NLRP3炎症小体在AD、VD、TBI中的作用,详细总结中药干预NLRP3炎症小体防治AD、VD、TBI的最新研究成果,以期传承中医药精华,为中医药临床防治认知功能障碍相关疾病提供参考和依据,同时为后续中医药防治认知功能障碍相关疾病的深入研究提供思路和方向。

关键词： NLRP3炎症小体阿尔茨海默病血管性痴呆创伤性脑损伤认知功能障碍中医药(TCM)

The formation of crystalline lithium sulfide on electrocatalytic surfaces in lithium-sulfur batteries

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Journal of Energy Chemistry 2022年第1期31卷 568-573,I0015页

作者： Yun-wei song Jin-Lei Qin Chang-Xin Zhao Meng Zhao Li-Peng Hou Yan-Qi Peng Hong-Jie Peng Bo-Quan Li Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengduSichuan 611731China

Lithium-sulfur(Li-S)battery is highly regarded as a promising next-generation energy storage device but suffers from sluggish sulfur redox *** the behavior and mechanism of the sulfur species on electrocatalytic surface is the first step to rationally introduce polysulfide electrocatalysts for kinetic promotion in a working ***,crystalline lithium sulfide(Li_(2)S)is exclusively observed on electrocatalytic surface with uniform spherical morphology while Li_(2)S on non-electrocatalytic surface is amorphous and *** characterization indicates the crystalline Li_(2)S preferentially participates in the discharge/charge process to render reduced interfacial resistance,high sulfur utilization,and activated sulfur redox ***,crystalline Li_(2)S is proposed with thermodynamic and kinetic advantages to rati on alize the superior performances of Li-S *** evoluti on of solid Li_(2)S on electrocatalytic surface not only addresses the polysulfide electrocatalysis strategy,but also inspires further investigation into the chemistry of energy-related processes.

关键词： Lithium-sulfur batteries Polysulfide electrocatalysis Lithium sulfide Framework porphyrin Sulfur redox reactions

Polydopamine-modified chitin conduits with sustained release of bioactive peptides enhance peripheral nerve regeneration in rats

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Neural Regeneration Research 2022年第11期17卷 2544-2550页

作者： Ci Li song-Yang Liu Li-Ping Zhou Tian-Tian Min Meng Zhang wei Pi Yong-Qiang Wen Pei-Xun Zhang Department of Orthopedics and Trauma Peking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural Regeneration Ministry of EducationBeijingChina National Center for Trauma Medicine BeijingChina Beijing Key Laboratory for Bioengineering and Sensing Technology School of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijingChina

The introduction of neurotrophic factors into injured peripheral nerve sites is beneficial to peripheral nerve ***,neurotrophic facto rs are rapidly degraded in vivo and obstruct axonal regeneration when used at a supraphysiological dose,which limits their clinical *** mimetic peptides have been developed to be used in place of neurotrophic factors because they have a similar mode of action to the original growth fa ctors and can activate the equivalent receptors but have simplified sequences and *** this study,we created polydopamine-modified chitin conduits loaded with brain-derived neurotrophic factor mimetic peptides and vascular endothelial growth fa ctor mimetic peptides(Chi/PDA-Ps).We found that the Chi/PDA-Ps conduits were less cytotoxic in vitro than chitin conduits alone and provided sustained release of functional *** this study,we evaluated the biocompatibility of the Chi/P DA-Ps ***-derived neurotrophic factor mimetic peptide and vascular endothelial growth fa ctor mimetic peptide synergistically promoted prolife ration of Schwann cells and secretion of neurotrophic factors by Schwann cells and attachment and migration of endothelial cells in *** Chi/P DA-Ps conduits were used to bridge a 2 mm gap between the nerve stumps in rat models of sciatic nerve *** found that the application of Chi/PDA-Ps conduits could improve the motor function of rats and reduce gastrocnemius *** electrophysiological results and the microstructure of regenerative nerves showed that the nerve conduction function and re myelination was further resto *** findings suggest that the Chi/PDA-Ps conduits have great potential in peripheral nerve injury repair.

关键词： angiogenesis bioactive peptides nerve repair neurotrophic factor peripheral nerve injury peripheral nerve regeneration polydopamine surface modification synergistic effects tissue engineering

西安市鄠邑区设施西瓜根结线虫种类鉴定及药剂防治研究

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中国农学通报 2024年第1期40卷 83-90页

作者：魏佩瑶潘嵩刘晨陈志杰张锋李英梅陕西省生物农业研究所/陕西省植物线虫学重点实验室西安710043

为明确陕西省西安市鄠邑区设施西瓜根结线虫发生危害情况以及病原种类,提出根结线虫病安全高效的药剂防治技术,实现陕西西瓜产业健康高效发展目标。于2022年5月在西安市鄠邑区主要设施西瓜种植基地,对根结线虫病害发生率、病情指数等进... 详细信息

为明确陕西省西安市鄠邑区设施西瓜根结线虫发生危害情况以及病原种类,提出根结线虫病安全高效的药剂防治技术,实现陕西西瓜产业健康高效发展目标。于2022年5月在西安市鄠邑区主要设施西瓜种植基地,对根结线虫病害发生率、病情指数等进行调查研究,并采集根部样品进行根结线虫分离,运用形态学及分子生物学技术鉴定不同产区设施西瓜根结线虫的种类,并对不同化学药剂防治效果进行比较。结果表明,鄠邑区西瓜主产区平均病田率达92.9%,病株率88.7%,病情指数73.1。2龄幼虫、雌成虫形态特征及形态测量指标和分子生物学鉴定结果均表明该地区根结线虫病病原为南方根结线虫。药剂试验表明,98%棉隆微粒剂防治效果显著,每公顷土壤施用150 kg为适宜的使用剂量,30 d的防效100%,70 d的防效92.1%;同时在西瓜移栽前用2250 mL/hm^(2)的41.7%氟吡菌酰胺进行土壤处理即可有效抑制南方根结线虫侵染,70 d后相对防效75.7%,效果优于药剂灌根。

关键词：根结线虫西瓜发生情况病原鉴定药剂防治鄠邑区

多房棘球蚴感染诱导肝窦内皮细胞发生毛细血管化的动态研究

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中国血吸虫病防治杂志 2024年第1期36卷 34-43页

作者：张仁杰谢俊卫繁娜莫筱瑾宋鹏蔡玉春卢艳孙家辉周魇林琳张颋陈木新中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)、传染病溯源预警与智能决策全国重点实验室、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、WHO热带病合作中心、科技部国家级热带病国际联合研究中心上海200025 海南热带病研究中心(国家热带病研究中心海南分中心) 海南海口571199 内蒙古大学生命科学学院广东省深圳市疾病预防控制中心广东深圳518073

目的观察泡型棘球蚴病发展过程中肝窦内皮细胞(liver sinusoidal endothelial cells,LSEC)毛细血管化现象及与肝纤维化的关系,为探索LSEC在泡型棘球蚴病所致肝损伤和肝纤维化发生和转归中的作用机制奠定基础。方法取40只C57BL/6小鼠(6~... 详细信息

目的观察泡型棘球蚴病发展过程中肝窦内皮细胞(liver sinusoidal endothelial cells,LSEC)毛细血管化现象及与肝纤维化的关系,为探索LSEC在泡型棘球蚴病所致肝损伤和肝纤维化发生和转归中的作用机制奠定基础。方法取40只C57BL/6小鼠(6~8周龄),随机分为对照组和感染1、2、4周组,每组各10只。感染1、2、4周组每只小鼠经肝脏直视穿刺注射接种2000个多房棘球蚴原头节,对照组用相同方法注射等量磷酸盐缓冲液。在感染后1、2、4周,取小鼠肝脏,经苏木精-伊红(hematoxylin-eosin,HE)染色后观察肝脏病理变化,经Masson三色染色后对染色阳性区域面积进行半定量分析以判断肝纤维化程度,采用免疫组织化学染色法评价α-平滑肌动蛋白(α-smooth muscle actin,α-SMA)和Ⅰ型胶原蛋白α1(collagen typeⅠalpha 1,COL1A1)表达水平以判断肝星状细胞(hepatic stellate cell,HSC)活化和细胞外基质(extracellular matrix,ECM)沉积,用扫描电子显微镜观察LSEC表面窗孔。从小鼠肝脏中分离原代LSEC,用实时荧光定量PCR(real-time fluorescence quantitative PCR,qPCR)法检测LSEC标志基因稳定素(Stabilin)-1、Stabilin-2、Ehd3、CD209b、GATA4、Maf mRNA表达水平。结果多房棘球蚴感染2周后,可见小鼠肝脏局部肝小叶结构被破坏;感染4周后,小鼠肝脏可见多房棘球蚴囊泡,周围有肉芽肿组织包绕。Masson三色染色半定量分析结果显示,4组小鼠肝脏内胶原纤维比例差异有统计学意义(F=26.060,P<0.001),感染4周组小鼠肝脏内胶原纤维比例[(11.29±2.58)%]较对照组增加(P<0.001)。免疫组织化学染色结果显示,感染1、2周组小鼠肝脏内见少量HSC活化和ECM沉积;感染4周组小鼠肝组织中可见大量棕黄色α-SMA和COL1A1聚集沉积在病变区域,并向周围扩散。半定量分析结果显示,4组小鼠肝脏内α-SMA和COL1A1表达水平差异均有统计学意义(F=7.667、6.530,P均<0.05),感染4周组小鼠肝脏内α-SMA[(7.13±3.68)%]和COL1A1[(13.18±7.20)%]表达水平均较对照组增加(P均<0.05)。扫描电子显微镜结果显示,4组小鼠LSEC开窗频率和孔隙率差异均有统计学意义(F=37.730、16.010,P均<0.001),感染1[分别为(1.22±0.48)个/μm^(2)、(3.05±0.91)%]、2周组[分别为(3.47±0.10)个/μm^(2)、(7.57±0.23)%]小鼠LSEC开窗频率和孔隙率均较对照组降低(P均<0.001)。4组小鼠LSEC平均窗孔直径差异有统计学意义(F=15.330,P<0.001),感染1[(180.80±16.42)nm]、2周组[(161.70±3.85)nm]小鼠LSEC平均窗孔直径均较对照组增加(P均<0.05)。4组小鼠LSEC中Stabilin-1、Stabilin-2、Ehd3、CD209b、GA TA4、Maf mRNA表达水平差异均有统计学意义(F=153.100、57.010、31.700、177.400、17.740、72.710,P均<0.001);与对照组相比,感染1、2、4周组小鼠上述基因相对表达量均降低(P均<0.001)。结论多房棘球蚴感染可诱导小鼠LSEC发生毛细血管化,并使LSEC功能和表型标志基因表达水平降低;LSEC毛细血管化早于HSC活化及肝纤维化发生。

关键词：多房棘球蚴泡型棘球蚴病肝窦内皮细胞毛细血管化肝纤维化

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Drip fertigation and plant hedgerows significantly reduce nitrogen and phosphorus losses and maintain high fruit yields in intensive orchards

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Journal of Integrative Agriculture 2023年第2期22卷 598-610页

作者： song Ke QIN Qin YANG Ye-feng SUN Li-juan SUN Ya-fei ZHENG Xian-qing Lü wei-guang XUE Yong Eco-Environmental Protection Research Institute Shanghai Academy of Agricultural SciencesShanghai 201403P.R.China Pudong New District Agro-Technology Extension Service Center Shanghai 201300P.R.China

A field experiment was carried out to evaluate the effects of drip fertigation combined with plant hedgerows on nitrogen and phosphorus runoff losses in intensive pear orchards in the Tai Lake *** and phosphorus runoff over a whole year were measured by using successional runoff water collection *** four experimental treatments were conventional fertilization(CK),drip fertigation(DF),conventional fertilization combined with plant hedgerows(C+H),and drip fertigation combined with plant hedgerows(D+H).The results from one year of continuous monitoring showed a significant positive correlation between precipitation and surface runoff *** runoff discharge under the treatments without plant hedgerows totaled 15.86%of precipitation,while surface runoff discharge under the treatments with plant hedgerows totaled 12.82%of *** hedgerows reduced the number of runoff events and the amount of surface *** is the main driving force for the loss of nitrogen and phosphorus in surface runoff,and fertilization is an important factor affecting the losses of nitrogen and *** CK,approximately 7.36%of nitrogen and 2.63%of phosphorus from fertilization entered the surface water through *** fertigation reduced the accumulation of nitrogen and phosphorus in the surface soil and lowered the runoff loss concentrations of total nitrogen(TN)and total phosphorus(TP).Drip fertigation combined with plant hedgerows significantly reduced the overall TN and TP losses by 45.38 and 36.81%,respectively,in comparison to the CK *** fertigation increased the vertical migration depth of nitrogen and phosphorus nutrients and reduced the accumulation of nitrogen and phosphorus in the surface soil,which increased the pear *** promotion of drip fertigation combined with plant hedgerows will greatly reduce the losses of nitrogen and phosphorus to runoff and maintain the high fruit yields in the intensive orchards of

关键词： drip fertigation plant hedgerows surface runoff nitrogen and phosphorus losses fruit yields

Secondary in-the-bag intraocular lens implantation combined with 120-and 360-degree goniotomy in glaucoma following congenital cataract surgery:a case report

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International Journal of Ophthalmology(English edition) 2023年第4期16卷 659-662页

作者： Yu Zhang Yue Zhou Yun-He song Xiu-Lan Zhang wei-Rong Chen State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina

Dear Editor,Glaucoma following cataract surgery(GFCS)is one of the most sight-threatening postoperative complications of pediatric cataract surgery,and often becomes refractory to medical treatment[1].Goniotomy has be... 详细信息

关键词： glaucoma surgery cataract