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ATP-citrate lyase regulates stemness and metastasis in hepatocellular carcinoma via the Wnt/β-catenin signaling pathway

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Hepatobiliary & Pancreatic Diseases International 2021年第3期20卷 251-261页

作者： Qin Han Ci-An Chen Wen Yang Dong Liang hong-wei lv Gui-Shuai lv Qian-Ni Zong hong-Yang Wang International Co-operation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghai 200438China National Center for Liver Cancer Research Shanghai 200000China Mengchao Hepatobiliary Hospital of Fujian Medical University Fuzhou 350108China State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteRenji HospitalShanghai Jiao Tong UniversityShanghai 200032China

Background: Hepatocellular carcinoma(HCC) is one of the most highly malignant tumors. Liver tumor-initiating cells(LTICs) have been considered to contribute to HCC progression and metastasis. ATP-citrate lyase(ACLY), as a key enzyme for de novo lipogenesis, has been reported to be upregulated in various tumors. However, its expression and role in HCC and LTICs remain unknown. Methods: The expressions of ACLY in HCC tissues were detected by quantitative real-time PCR(q RT-PCR), Western blotting and immunohistochemistry. Kaplan-Meier curves and Chi-square test were used to determine the clinical significance of ACLY expression in HCC patients. A series of assays were performed to determine the function of ACLY on stemness, migration and invasion of HCC cells. Luciferase reporter assay, Western blotting and immunoprecipitation were used to study the regulation of the Wnt/β-catenin signaling by ACLY. Rescue experiments were performed to investigate whether β-catenin was the mediator of ACLY-regulated stemness and migration in HCC cells. Results: ACLY was highly expressed in HCC tissues and LTICs. Overexpression of ACLY was significantly correlated with poor prognosis, progression and metastasis of HCC patients. Knockdown of ACLY remarkably suppressed stemness properties, migration and invasion in HCC cells. Mechanistically, ACLY could regulate the canonical Wnt pathway by affecting the stability of β-catenin, and Lys49 acetylation of β-catenin might mediate ACLY-regulated β-catenin level in HCC cells. Conclusions: ACLY is a potent regulator of Wnt/β-catenin signaling in modulating LTICs stemness and metastasis in HCC. ACLY may serve as a new target for the diagnosis and treatment of HCC.

关键词： Hepatocellular carcinoma ATP-citrate lyase Liver tumor-initiating cells Metastasis β-catenin

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Effectiveness of a hydroxynaphthoquinone fraction from Arnebia euchroma in rats with experimental colitis

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World Journal of Gastroenterology 2013年第48期19卷 9318-9327页

作者： Hua-Ying Fan Zi-Liang Zhang Ke Liu Ming-Yan Yang wei-hong lv Xin Che Hui Xu wei-wei Song School of Pharmacy Yantai University College of Life Science Jilin University Shandong Target Drug Research Co. Ltd.

AIM:To evaluate the potential effectiveness of hydroxynaphthoquinone mixture(HM)in rats with 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced ***:Colitis was induced by intracolonic administration of TNBS(80 mg/kg,dissolved in 50%ethanol).Rats were treated daily for 7 d with HM(2.5,5,10 mg/kg)and mesalazine 100 mg/kg 24 h after TNBS *** progression was monitored daily by observation of clinical signs and body weight *** the end of the experiment,macroscopic and histopathologic lesions of rats were scored,and myeloperoxidase(MPO)activity was *** also determined inflammatory cytokine tumor necrosis factor(TNF)-αlevel by ELISA,Western blotting and immunochemistry to explore the potential mechanisms of ***:After intracolonic instillation of TNBS,animals developed colitis associated with soft stool,diarrhea and marked colonic *** of HM significantly attenuated clinical and histopathologic severity of TNBS-induced colitis in a dose-dependent *** abrogated body weight loss,diarrhea and inflammation,decreased macroscopic damage score,and improved histological signs,with a significant reduction of inflammatory infiltration,ulcer size and the severity of goblet cell depletion(all P<0.05 vs TNBS alone group).HM could reduce MPO *** addition,it also decreased serum TNF-αlevel and down-regulated TNF-αexpression in colonic *** reduction was statistically significant when the dose of HM was 10 mg/kg(P<0.05 vs TNBS alone group),and the effect was comparable to that of mesalazine and showed no apparent adverse *** underlying mechanism may be associated with TNF-α***:These findings suggest that HM possesses favourable therapeutic action in TNBS-induced colitis,which provides direct pharmacological evidence for its clinical application.

关键词： Arnebia euchroma(Royle) Johnst Hydroxynaphthoquinones Inflammatory bowel disease 2,4,6-trinitrobenzene sulfonic acid-induced colitis Tumor necrosis factor

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Puerarin enhances superoxide dismutase activity and inhibits RAGE and VEGF expression in retinas of STZ-induced early diabetic rats

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Asian Pacific Journal of Tropical Medicine 2012年第11期5卷 891-896页

作者： Fang Chen hong-Quan Zhang Jun Zhu Kai-Yang Liu hong Cheng Guo-Li Li Shan Xu wei-hong lv Zheng-Gao Xie Department of Ophthalmology Clinical Medicine CollegeYangzhou University Institute of Pharmacy School of MedicineYangzhou University Department of Biochemistry School of MedicineYangzhou University Department of Pathology School of MedicineYangzhou University

Objective:To investigate the effects of puerarin on the activity of superoxide dismutase(SOD), and expressions of advanced glycation end-product(AGE) receptor(RAGE) and vascular endothelial growth factor(VEGF) in retinas of streptozotocin(STZ)-induced early diabetic rats. Methods:Diabetic rat models were established by inducing diabetes via intra-peritoneal injection of *** were randomly divided into normal(control),diabetic(DM),and DM+ puerarin *** intra-gastric administration of puerarin(500 mg/kg/day for 4 weeks),levels of SOD and malondialdehyde(MDA) were determined in serum and *** and protein expression levels of RAGE and VEGF in retinas were determined by real-lime polymerase chain reaction(RT-PCR)(mRNA) and Western blot analysis(protein levels).Results:There was significantly lower SOD activity and significantly higher MDA in serum and retinas of the DM group compared with the two other groups(P<0.05).After treatment with puerarin,SOD activity increased and MDA content decreased in this group(P<0.05).mRNA and protein expression levels of RACE and VECF in the DM group were significantly higher than those of the other groups (P<0.05),and decreased after puerarin treatment(P<0.05).Conclusions:Puerarin is able to enhance SOD activity,and inhibit RAGE and VEGF expressions in retinas of STZ-induced early diabetic ruts.

关键词： Diabetic retinopathy Puerarin Oxidative stress Receptor for advanced glycation end products Vascular endothelial growth factor

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Protein Templated Au-CuO Bimetallic Nanoclusters toward Neutral Glucose Sensing

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Chinese Journal of Chemical Physics 2022年第3期35卷 570-577,I0003页

作者： hong-wei lv Quan-fu Li Hui-ling Peng College of Electronic Engineering Guangxi Normal UniversityGuilin 541004China

In this study,the application of bovine serum albumin(BSA)as a carrier to glucose-sensitive materials for the detection of glucose was ***-Cu O bimetallic nanoclusters(Au-Cu O/BSA)were prepared using BSA as a template,the new sensing material(Au-Cu O/BSA/MWCNTs)was synthesized by mixing with multi-walled carbon nanotubes(MWCNT)and applied to non-enzymatic electrochemical sensors to detect glucose stably and effectively under neutral *** scanning electron microscopy was used to investigate the morphology of the synthesized *** electrochemical properties of the sensor were studied by cyclic *** detection experiments show that Au-Cu O/BSA/MWCNTs/Au electrode has good glucose detection ability,stability,accuracy,repeatability,and high selectivity in neutral *** existing glucose-sensitive materials,due to the use of BSA,the composite material is firmly fixed to the electrode surface without a Nafion solution,which reduces the current blocking effect on the modified *** composite materials can be effectively preserved for extremely long periods,higher than 80%activity is maintained at room temperature in a closed environment for 3 to 4 months,due to the special effects of *** addition,the feasibility of using BSA in glucose-sensitive materials is confirmed.

关键词： Bovine serum albumin Non-enzymatic sensor Glucose detection Bimetallic nanocluster Neutral condition

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Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori

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World Journal of Gastroenterology 2012年第48期18卷 7212-7224页

作者： Fen Wang Li-Dan Luo Jian-Hua Pan Li-Hua Huang hong-wei lv Qin Guo Can-Xia Xu Shou-Rong Shen Department of Gastroenterology The Third Xiangya HospitalCentral South University Department of Gastroenterology Hunan Provincial Hospital

AIM:To identify genes potentially involved in Helicobacter pylori(***)-induced gastric ***:GES-1 cells were co-cultured with *** strains isolated from patients with gastric carcinoma(GC,n = 10) or chronic gastritis(CG,n = 10) for in vitro proliferation and apoptosis assays to identify the most and least virulent *** two strains were cagA-genotyped and used for further in vivo carcinogenic virulence assays by infecting Mongolian gerbils for 52 wk,respectively;a broth free of *** was lavaged as *** profiles of GES-1 cells cocultured with the most and least virulent strains were determined by microarray *** most differentially expressed genes were further verified using quantitative real-time polymerase chain reaction in GES-1cells infected with the most and least virulent strains,and by immunohistochemistry in *** positive CG,precancerous diseases,and GC biopsy specimens in an independent ***:GC-derived *** strains induced a potent proliferative effect in GES-1 cells in co-culture,whereas CG-derived strains did *** most(from a GC patient) and least(from a CG patient) virulent strains were cagA-positive and negative,*** week 52,CG,atrophy,metaplasia,dysplasia,and GC were observed in 90.0%,80.0%,80.0%,90%,and 60.0%,respectively,of the animals lavaged with the most virulent ***,only mild CG was observed in 90% of the animals lavaged with the least virulent *** microarray analysis,800 differentially expressed genes(49 up-and 751 down-regulated),involving those associated with cell cycle regulation,cell apoptosis,cytoskeleton,immune response,and substance and energy metabolisms,were identified in cells co-cultured with the most virulent strain as compared with those co-cultured with the least virulent *** six most differentially expressed genes(with a betweenness centrality of 0.1-0.2) were identified among the significant differential gene prof

关键词： Helicobacter pylori Gastric carcinoma Proliferation Genomic profiles

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A mild, configurable, flexible CoNi-LDH（v）/Zn battery based on H-vacancy-induced reversible Zn2+ intercalation

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Journal of Energy Chemistry 2025年第1期100卷 498-508页

作者： Xinyue Cheng Dingsheng Wu Hao Gao Qingqing Wang Pengfei lv Sam S.Yoon Qufu wei Key Laboratory of Eco-textiles Ministry of Education Jiangnan University School of Mechanical Engineering Korea University

Flexible Zn-based batteries have attracted increasing research interest as essential components of wearable energy storage ***,the advancement of flexible aqueous Zn-based batteries based on Co-Ni layered double hydroxide （CoNi-LDH） as the cathode material is hampered by their poor cycling stability and the corrosiveness of alkaline ***,CoNi-LDH nanosheets enriched with H vacancies (CoNi-LDH（v）) were constructed on a flexible carbon cloth （CC） substrate via electrochemical deposition and *** Zn-based battery comprising CoNi-LDH（v）@CC as the cathode exhibited highly reversible conversion reactions and stable operation in 3 M ZnSO4electrolyte （pH=4）.The battery delivered an excellent specific capacity (225 mA h g-1,0.26 mA h cm-2),acceptable cycling stability（53.9%,900 cycles）,and high discharging *** abundant H vacancies served as active sites for the reversible intercalation of Zn2+and the extravasation of NO3-generated channels and space for Zn2+transport and storage,together enabling an excellent Zn2+storage ***,a sandwich-structured solid-state CoNi-LDH（v）@CC//Zn@CC battery was fabricated and was found to exhibit a noteworthy electrochemical performance and mechanical *** a proof of concept,the unencapsulated battery powered a digital watch under various deformation conditions and operated stably for 80 ***,the flexible battery displayed outstanding customizability,maintaining an open-circuit voltage of 1.42 V even after being cut *** proposed engineering strategy contributes to the realization of textiles with truly wearable energy-storage devices.

关键词： CoNi/Zn battery H vacancies Mild aqueous electrolyte Tailorable battery Wearable energy storage device

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Recent progress in constructing fluorinated solid-electrolyte interphases for stable lithium metal anodes

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 270-291页

作者： Di Zhang Pengfei lv wei Qin Xin He Yuanhua He Civil Aircraft Fire Science and Safety Engineering Key Laboratory of Sichuan Province College of Civil Aviation Safety EngineeringCivil Aviation Flight University of ChinaGuanghan 618307China School of Chemical Engineering Sichuan UniversityChengdu 610065China

Lithium metal batteries(LMBs)are emerging as a promising energy storage solution owing to their high energy density and specific ***,the non-uniform plating of lithium and the potential rupture of the solid-electrolyte interphase(SEI)during extended cycling use may result in dendrite growth,which can penetrate the separator and pose significant short-circuit *** a stable SEI is essential for the long-term operation of the ***-rich SEI has garnered significant attention for its ability to effectively passivate electrodes,regulate lithium deposition,and inhibit electrolyte *** the structural components and preparation methods of existing fluorinated SEI is crucial for optimizing lithium metal anode *** paper reviews the research on optimizing LiF passivation interfaces to protect lithium metal *** focuses on four types of compositions in fluorinated SEI that work synergistically to enhance SEI *** instance,combining compounds with LiF can further enhance the mechanical strength and ionic conductivity of the *** metals with LiF significantly improves electrochemical performance at the SEI/anode interface,with a necessary focus on reducing electron tunneling ***,incorporating polymers with LiF offers balanced improvements in interfacial toughness and ionic conductivity,though maintaining structural stability over long cycles remains a critical area for future *** alloys combined with LiF increase surface energy and lithium affinity,challenges such as dendrite growth and volume expansion *** summary,this paper emphasizes the crucial role of interfacial structures in LMBs and offers comprehensive guidance for future design and development efforts in battery technology.

关键词： LiF lithium metal anodes solid-electrolyte interphase interface cycling stability

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Blood diagnostic and prognostic biomarkers in amyotrophic lateral sclerosis

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Neural Regeneration Research 2025年第9期20卷 2556-2570页

作者： Yongting lv hongfu Li Department of Neurology Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina Department of Medical Genetics and Center for Rare disease Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina Nanhu Brain-Computer Interface Institute HangzhouZhejiang Province

Amyotrophic lateral sclerosis is a devastating neurodegenerative disease for which the current treatment approaches remain severely *** principal pathological alterations of the disease include the selective degeneration of motor neurons in the brain,brainstem,and spinal cord,as well as abnormal protein deposition in the cytoplasm of neurons and glial *** biological markers under extensive scrutiny are predominantly located in the cerebrospinal fluid,blood,and even *** these biomarke rs,neurofilament proteins and glial fibrillary acidic protein most accurately reflect the pathologic changes in the central nervous system,while creatinine and creatine kinase mainly indicate pathological alterations in the peripheral nerves and *** light chain levels serve as an indicator of neuronal axonal injury that remain stable throughout disease progression and are a promising diagnostic and prognostic biomarker with high specificity and ***,there are challenges in using neurofilament light chain to diffe rentiate amyotrophic lateral sclerosis from other central nervous system diseases with axonal *** fibrillary acidic protein predominantly reflects the degree of neuronal demyelination and is linked to non-motor symptoms of amyotrophic lateral sclerosis such as cognitive impairment,oxygen saturation,and the glomerular filtration *** DNA-binding protein 43,a pathological protein associated with amyotrophic lateral sclerosis,is emerging as a promising biomarker,particularly with advancements in exosome-related *** is currently lacking for the value of creatinine and creatine kinase as diagnostic markers;however,they show potential in predicting disease *** the vigorous progress made in the identification of amyotrophic lateral sclerosis biomarkers in recent years,the quest for definitive diagnostic and prognostic biomarke rs remains a formidable *** review summarizes the lat

关键词： amyotrophic lateral sclerosis biomarker blood biomarkers diagnosis glial fibrillary acidic protein neurofilament light chain prognosis TAR DNA-binding protein 43

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Rhenium-boosted electrocatalytic activity and durability of pyrolytic IrO2 for acidic oxygen evolution

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Rare Metals 2024年第12期 6758-6764页

作者： hong-wei lv hong-Bin Zhao Xin-Yuan Peng Zhi-Guo Ye Quan-Bo Huang Xue-Tao Yuan Duo-Sheng Li Zhong Jin School of Materials Science and Engineering Nanchang Hangkong University General Research Institute for Nonferrous Metals China GRINM Group Co. Ltd. National Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals GRINM Resources and Environment Tech.Co.Ltd.Beijing Engineering Research Center of Strategic Nonferrous Metals Green Manufacturing Technology State Key Laboratory of Coordination Chemistry MOE Key Laboratory of Mesoscopic ChemistryMOE Key Laboratory of High Performance Polymer Materials and TechnologyJiangsu Key Laboratory of Advanced Organic MaterialsSchool of Chemistry and Chemical EngineeringNanjing University

IrO2,renowned for its exceptional stability in acidic media,suffers from limited electrocatalytic activity for the oxygen evolution reaction （OER）.To enhance the intrinsic OER activity while preserving ultrahigh stability,here we report the synthesis of rhenium （Re） heteroatom incorporated pyrolytic IrO2,using the pyrolytic decomposition method.

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The pivotal role of microglia in injury and the prognosis of subarachnoid hemorrhage

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Neural Regeneration Research 2025年第7期20卷 1829-1848页

作者： Wenjing Ning Shi lv Qian Wang Yuzhen Xu Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina Department of Central Laboratory The Affiliated Taian City Central Hospital of Qingdao UniversityTaianShandong ProvinceChina

Subarachnoid hemorrhage leads to a series of pathological changes,including vascular spasm,cellular apoptosis,blood–brain barrier damage,cerebral edema,and white matter ***,which are the key immune cells in the central nervous system,maintain homeostasis in the neural environment,support neurons,mediate apoptosis,participate in immune regulation,and have neuroprotective *** evidence has shown that microglia play a pivotal role in the pathogenesis of subarachnoid hemorrhage and affect the process of injury and the prognosis of subarachnoid ***,microglia play certain neuroprotective roles in the recovery phase of subarachnoid *** approaches aimed at modulating microglia function are believed to attenuate subarachnoid hemorrhage *** provides new targets and ideas for the treatment of subarachnoid ***,an in-depth and comprehensive summary of the role of microglia after subarachnoid hemorrhage is still *** review describes the activation of microglia after subarachnoid hemorrhage and their roles in the pathological processes of vasospasm,neuroinflammation,neuronal apoptosis,blood–brain barrier disruption,cerebral edema,and cerebral white matter *** also discusses the neuroprotective roles of microglia during recovery from subarachnoid hemorrhage and therapeutic advances aimed at modulating microglial function after subarachnoid ***,microglia in subarachnoid hemorrhage are targeted with TLR inhibitors,nuclear factor-κB and STAT3 pathway inhibitors,glycine/tyrosine kinases,NLRP3 signaling pathway inhibitors,Gasdermin D inhibitors,vincristine receptorαreceptor agonists,ferroptosis inhibitors,genetic modification techniques,stem cell therapies,and traditional Chinese ***,most of these are still being evaluated at the laboratory *** clinical studies and data on subarachnoid hemorrhage are required to improve the treatment of subarachnoid hemor

关键词： apoptosis blood–brain barrier brain edema microglia neuroinflammation neuron neuroprotection subarachnoid hemorrhage vasoconstriction white matter injury

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