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Emerging approaches to probing ion channel structure and function

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Neuroscience Bulletin 2012年第4期28卷 351-374页

作者： wei-guang li Tian-Le Xu Neuroscience Division Department of Biochemistry and Molecular Cell BiologyInstitute of Medical SciencesShanghaiJiao Tong University School of MedicineShanghai 200025China

Ion channels,as membrane proteins,are the sensors of the *** act as the first line of communication with the world beyond the plasma membrane and transduce changes in the external and internal environments into unique electrical signals to shape the responses of excitable *** of their importance in cellular communication,ion channels have been intensively studied at the structural and functional ***,we summarize the diverse approaches,including molecular and cellular,chemical,optical,biophysical,and computational,used to probe the structural and functional rearrangements that occur during channel activation（or sensitization）,inactivation（or desensitization）,and various forms of *** emerging insights into the structure and function of ion channels by multidisciplinary approaches allow the development of new pharmacotherapies as well as new tools useful in controlling cellular activity.

关键词： ion channel structure and function mutagenesis covalent modification voltage-clamp fluorometry computational chemistry

Pyrolysis Characteristics and Kinetics of the Preparation Process of Sludge-Based Activated Carbon by ZnCl_2 Activation Method

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Journal of Harbin Institute of Technology(New Series) 2013年第6期20卷 29-36页

作者： Xin li guang-Zhi Wang wei-guang li Ping Wang School of Municipal and Environmental Engineering Harbin Institute of Technology State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

To obtain the pyrolysis characteristics and kinetics of preparation process of sludge-based activated carbon by ZnCl2 activation method （*** pyrolysis process of the sludge with ZnCl2 activation）,the characteristic of mass loss and gas products generated during pyrolysis of the sludge with ZnCl2 activation were analyzed by thermogravimetric analysis coupled with Fourier Transform Infrared Spectroscopy （TG-FTIR）.The kinetic parameters were calculated by the Coats-Redfem method and the mechanism models were *** role of ZnCl2 in the pyrolysis process of the sludge with ZnCl2 activation was also illustrated through the comparison of the pyrolysis characteristics and kinetics of the sludge with and without ZnCl2 *** results showed that the pyrolysis process of the sludge with ZnCl2 activation can be divided into four stages including the dehydration of sludge and initial depolymerization of a small portion of organics matters,the decomposition of large molecular organic matters into small molecular intermediates,the further degradation of intermediates and volatilization of ZnCl2,and the decomposition of inorganic minerals and undecomposed organic ***2,CO,CH4,H2O,some aldehydes and carboxylic acids are the major pyrolysis gaseous *** activation energies and pre-exponential factors are in the range of 28.84-206.42 kJ/mol and 9885.16-8.08× 1011 min-1,*** the pyrolysis of sludge,ZnC12 not only can function as a dehydration agent and inhibit the formation of tar,but also can peptize the organic matters in the sludge,making them easier to be decomposed.

关键词： sludge ZnCl2 activation pyrolysisCLC number:X705 Document code:AArticle ID:1005-9113(2013)06-0029-08

Memory Trace for Fear Extinction:Fragile yet Reinforceable

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Neuroscience Bulletin 2024年第6期40卷 777-794页

作者： Ying liu Shuai Ye Xin-Ni li wei-guang li Department of Rehabilitation Medicine State Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain ScienceHuashan HospitalInstitute for Translational Brain ResearchFudan UniversityShanghai200032China

Fear extinction is a biological process in which learned fear behavior diminishes without anticipated reinforcement,allowing the organism to re-adapt to ever-changing *** on the behavioral hypothesis that extinction is new learning and forms an extinction memory,this new memory is more readily forgettable than the original fear *** brain’s cellular and synaptic traces underpinning this inherently fragile yet reinforceable extinction memory remain *** questions are about the whereabouts of the engram neurons that emerged during extinction learning and how they constitute a dynamically evolving functional construct that works in concert to store and express the extinction *** this review,we discuss recent advances in the engram circuits and their neural connectivity plasticity for fear extinction,aiming to establish a conceptual framework for understanding the dynamic competition between fear and extinction memories in adaptive control of conditioned fear responses.

关键词： Fear extinction memory Memory trace Fear relapse Medial prefrontal cortex Basolateral amygdala Ventral hippocampus Insular cortex Synaptic adaptations

Radar target recognition based on micro-Doppler effect

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Optoelectronics Letters 2008年第6期4卷 456-459页

作者： DONG wei-guang li Yan-jun College of Astronautics Northwestern Polytechnical University Xi ＇an 710072 China

Mechanical vibration of target structures will modulate the phase function of radar backscattering,and will induce the frequency modulation of returned signals from the target. It generates a side bands of the target body Doppler frequency shift,which is helpful for target recognition. Based on this,a micro-Doppler atomic storehouse is built for the target recognition,and four kinds of common classifiers are used separately to perform the classified recognition. The simulation experimental results show that this method has high recognition rate above 90%.

关键词：雷达目标显微多普勒效应信息处理后向散射

Molecular and functional characterization of three odorant-binding proteins from the wheat blossom midge,Sitodiplosis mosellana

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Insect Science 2020年第4期27卷 721-734页

作者： wei-Ning Cheng Yu-Dong Zhang wei liu guang-wei li Keyan Zhu-Salzman Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau Ministry of AgricultureNorthwest A&F UniversityYang lingShaanxiChina Shaanxi Key Laboratory of Chinese Jujube Van'an UniversityVan'anShaanxiChina Department of Entomology Texas A&M UniversityCollege StationTXUSA

Sitodiplosis mosellana,a periodic but devastating wheat pest,relies on wheat spike volatiles as a cue in sclecing hosts for *** odorant-binding proteins(OBPs)are thought to play essential roles in filtering,binding and transporting hydropho-bic odorant molecules to specific *** date,the molecular mechanisms underlying *** olfaction are poorly ***,three *** antenna-specific OBP genes,SmosOBPII,16 and 21,were cloned and bacterially *** properties of the recombinant proteins to 28 volatiles emitted from wheat spikes were in-vestigated using fluorescence competitive binding *** analysis suggested that these SmosOBPs belong to the Classic OBP ***-binding analysis showed that all three SmosOBPs preferentially bound alcohol,ester and ketone com-pounds,and SmosOBP11 and 16 also selectively bound terpenoid *** par-ticular,the three SmosOBPs had high binding affinities(Kilicited strong electroantennogram(EAG)response fromfemale *** addition,SmosOBP11 displayed significantly higher binding(Kilicited significant EAG response,suggesting that SmosOBP11 plays a ma-jor role in recognition and transportation of these *** findings have provided important insight into the molecular mechanism by which *** specifically rec-ognizes plant volatiles for host selection,and have facilitated identification of effective volatile attractants that are potentially useful for pest monitoring and trapping.

关键词： binding assay electroantennogram response host selection odorant-binding protein Sitodiplosis mosellana wheat spike violates

Segregating memories: targeting microenvironment of neuronal ensembles

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Signal Transduction and Targeted Therapy 2022年第11期7卷 3995-3997页

作者： Ying liu Miao Xu wei-guang li Department of Rehabilitation Medicine Huashan HospitalInstitute for Translational Brain ResearchState Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain ScienceFudan University200032ShanghaiChina

A recent study by Shen et *** that a delayed augmentation in the neuronal expression of C–C chemokine receptor type 5(CCR5)—a well-known immune molecule in the peripheral inflammatory microenvironment—after the acquisition of a contextual memory determines the duration of the temporal window for linking with another memory,effectively elucidating mechanisms that shape the memory linking timescales.

关键词： linking ensemble dating

GPU-based cross-platform Monte Carlo proton dose calculation engine in the framework of Taichi

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Nuclear Science and Techniques 2023年第5期34卷 152-162页

作者： wei-guang li Cheng Chang Yao Qin Zi-Lu Wang Kai-Wen li li-Sheng Geng Hao Wu School of Physics Beihang UniversityBeijing 102206China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Radiation OncologyPeking University Cancer Hospital and Institute52 Fucheng RoadBeijing 100142China Medical Management Department CAS Ion Medical Technology Co.Ltd.Beijing 100190China Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang UniversityBeijing 102206China School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China Institute of Medical Technology Peking University Health Science Center38 Xueyuan RoadBeijing 100191China

In recent years,graphics processing units(GPUs)have been applied to accelerate Monte Carlo(MC)simulations for proton dose calculation in ***,current GPU platforms,such as Compute Unified Device Architecture(CUDA)and Open Computing Language(OpenCL),suffer from cross-platform limitation or relatively high programming ***,the Taichi toolkit,which was developed to overcome these difficulties,has been successfully applied to high-performance numerical *** on the class II condensed history simulation scheme with various proton-nucleus interactions,we developed a GPU-accelerated MC engine for proton transport using the Taichi *** distributions in homogeneous and heterogeneous geometries were calculated for 110,160,and 200 MeV protons and were compared with those obtained by full MC simulations using *** gamma passing rates were greater than 0.99 and 0.95 with criteria of 2 mm,2%and 1 mm,1%,respectively,in all the benchmark ***,the calculation speed was at least 5800 times faster than that of TOPAS,and the number of lines of code was approximately 10 times less than those of CUDA or *** study provides a highly accurate,efficient,and easy-to-use proton dose calculation engine for fast prototyping,beamlet calculation,and education purposes.

关键词： Proton therapy Monte Carlo dose calculation GPU acceleration Taichi

Cloning and Characterization of Porcine TSARG7 Gene and Analysis of Its Tissue-Specific Expression

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Agricultural Sciences in China 2009年第11期8卷 1384-1391页

作者： li Mei-li li Gui-qiang FANG wei WANG wei SONG Xiao-guang li Er-lin JIA Chao XU Yin-xue College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 P.R.China

TSARG7 is a novel member of the acyltransferase family since its sequence possesses the highly conserved phosphate acyltransferase （PlsC） domain existing in all acyltransferase-like proteins. The porcine TSARG7 had been identified by cloning in silico but had not been confirmed experimentally. The full-length mRNA of porcine TSARG7 gene was sequenced and two splice variants were discovered. The full-length cDNA of TSARG7 variant 1 was 2 513 bp and variant 2 was 2 634 bp. The putative porcine TSARG7 proteins, which were located in the cytoplasm, encoded 458 and 456 amino acids, respectively. Real-time PCR analysis showed that TSARG7 gene was expressed in various tissues, but at different levels. The expression levels of this gene were higher in the skeletal muscle, heart, and testis than that in other tissues, suggesting that the TSARG7 gene played a role in procine skeletal muscle, heart, and testis functions.

关键词： TSARG7 cDNA cloning pig bioinformatic analysis gene expression

High dose glargine alters the expression profiles of microRNAs in pancreatic cancer cells

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World Journal of Gastroenterology 2012年第21期18卷 2630-2639页

作者： wei-guang li Yao-Zong Yuan Min-Min Qiao Yong-Ping Zhang Department of Gastroenterology Ruijin HospitalSchoolof MedicineShanghai Jiaotong UniversityShanghai 200025China

AIM: To investigate the effect of high dose glargine on the expression profiles of microRNAs in human pancreatic cancer cells. METHODS: Real-time polymerase chain reaction array (RT-PCR) was applied to investigate miRNAs differentially expressed in Sw1990 cells treated with or without 100 IU/L glargine. Stem-loop RT-PCR was used to confirm the results of the array assay in Sw1990 and Panc-1 cells. The effects of miR-95 on cell growth, apoptosis, invasion and migration abilities were respectively examined by CCK8 assay, apoptosis assay, Matrigel invasion and migration assay in Sw1990 and Panc-1 cells. Nude mice xenograft models with Sw1990 cells were built to investigate pancreatic cancer growth in vivo after transfection by the lentivirus pGLV3-GFP-miR-95. RESULTS: Ten miRNAs were significantly up-regulated and 2 miRNAs down-regulated in glargine treated Sw1990 cells when compared with non-treated cells (2.48-fold changes on average, P liferation (Sw1990: 2.510 ± 0.129 vs 2.305 ± 0.187, P < 0.05; Panc-1: 2.439 ± 0.211 vs 2.264 ± 0.117, P < 0.05), invasion (Sw1990: 67.90 ± 12.33 vs 47.30 ± 5.89, P < 0.01; Panc-1: 37.80 ± 8.93 vs 30.20 ± 5.14, P < 0.01), migration (Sw1990: 101 ± 6.00 vs 51.20 ± 8.34, P < 0.01; Panc-1: 91.80 ± 9.22 vs 81.50 ± 7.47, P < 0.01) and inhibited cell apoptosis (Sw1990: 22.05% ± 1.92% vs 40.32% ± 1.93%, P < 0.05; Panc-1: 20.17% ± 0.85% vs 45.60% ± 1.43%, P < 0.05) when compared with paired negative controls, whereas knockdown of miR-95 obtained the opposite effect. Nude mice xenograft models confirmed that miR-95 promoted the

关键词： Glargine MicroRNAs Pancreatic cancer Lentivirus Cancer growth