Cloning and Characterization of Porcine TSARG7 Gene and Analysis of Its Tissue-Specific Expression

作     者：LI Mei-li LI Gui-qiang FANG Wei WANG Wei SONG Xiao-guang LI Er-lin JIA Chao XU Yin-xue 

作者机构：College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2009年第8卷第11期

页      面：1384-1391页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0901[农学-作物学] 0902[农学-园艺学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National High Technology Research and Development Program of China(2006AA10Z136) 

主　　题：TSARG7 cDNA cloning pig bioinformatic analysis gene expression 

摘      要：TSARG7 is a novel member of the acyltransferase family since its sequence possesses the highly conserved phosphate acyltransferase （PlsC） domain existing in all acyltransferase-like proteins. The porcine TSARG7 had been identified by cloning in silico but had not been confirmed experimentally. The full-length mRNA of porcine TSARG7 gene was sequenced and two splice variants were discovered. The full-length cDNA of TSARG7 variant 1 was 2 513 bp and variant 2 was 2 634 bp. The putative porcine TSARG7 proteins, which were located in the cytoplasm, encoded 458 and 456 amino acids, respectively. Real-time PCR analysis showed that TSARG7 gene was expressed in various tissues, but at different levels. The expression levels of this gene were higher in the skeletal muscle, heart, and testis than that in other tissues, suggesting that the TSARG7 gene played a role in procine skeletal muscle, heart, and testis functions.