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Silencing Bmi-1 enhances the senescence and decreases the metastasis of human gastric cancer cells

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World Journal of Gastroenterology 2013年第46期19卷 8764-8769页

作者： Feng-Lan Gao wei-shan li Chun-ling liu Guo-Qiang Zhao Pathological Examination and Research Center Luohe Medical College Basic Medical Science Institute Zhengzhou University

AIM:To evaluate the impact of Bmi-1 on cell senescence and metastasis of human gastric cancer cell line ***:Two pairs of complementary small hairpin RNA(shRNA)oligonucleotides targeting the Bmi-1gene were designed,synthesized,annealed and cloned into the pRNAT-U6.2 *** DNA sequencing to verify the correct insertion of the shRNA sequences,the recombinant plasmids were transfected into BGC823 *** expression of Bmi-1 mRNA and protein was examined by reverse transcription-polymerase chain reaction(RT-PCR)and Western *** effects of Bmi-1 knockdown on cell senescence and metastasis were determined by theβ-Gal activity assay and Boyden chamber assay,***:The double-stranded oligonucleotide fragments of Bmi-1 short interfering RNA(siRNA)cloned into pRNAT-U6.2 vector conformed to the inserted ***-PCR and Western blotting indicated that the expression levels of Bmi-1 gene mRNA and protein were markedly decreased in transfected BGC823 cells with pRNAT-U6.2-si1104 and pRNATU6.2-si1356,especially in transfected BGC823 cells with pRNAT-U6.2-si1104,compared with two control groups(empty vector and blank group).In particular,Bmi-1 protein expression was almost completely abolished in cells transfected with the recombinant vector harboring shRNA targeting the sequence GGAGGAGGTGAATGATAAA(nt1104-1122).Compared with untransfected cells and cells transfected with the empty vector,the mean percentage of senescent cells increased and the number of cells passing through the Matrigel decreased in cells transfected with the recombinant ***:Silencing Bmi-1 by RNA interference can increase the senescent cell rate and effectively reduce the metastasis of gastric cancer cells.

关键词： Bmi-1 Gastric cancer Senescence Metas-tasis

Synthesis of dandelion-like TiO_2 microspheres as anode materials for lithium ion batteries with enhanced rate capacity and cyclic performances

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International Journal of Minerals,Metallurgy and Materials 2012年第11期19卷 1058-1062页

作者： Jin Yi Yan-lin liu Yuan Wang Xiao-ping li She-jun Hu wei-shan li School of Chemistry and Environment South China Normal University Guangzhou 510006 China Key Laboratory of Electrochemical Technology on Energy Storage and Power Generation of Guangdong Higher Education Institutes Guangzhou 510006 China Engineering Research Center of Materials and Technology for Electrochemical Energy Storage Ministry of Education Guangzhou 510006 China

Dandelion-like TiO2 microspheres consisting of numerous rutile single-crystalline nanorods were synthesized for the first time by a hydrothermal method. Their crystal structure, morphology and electrochemical properties were characterized by X-ray diffraction （XRD）, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, and galvanostatic charge and discharge tests. The results show that the synthesized TiO2 microspheres exhibit good rate and cycle performances as anode materials of lithium ion batteries. It can be found that the dandelion-like structure provides a larger specific surface area and the single-crystalline nanorod provides a stable structure and fast pathways for electron and lithium ion transport, which contribute to the rate and cycle performances of the battery.

关键词： titanium dioxide microspheres nanorods anodes lithium batteries

Therapeutic effect of autologous concentrated growth factor on lower-extremity chronic refractory wounds:A case report

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World Journal of Clinical Cases 2021年第18期9卷 4797-4802页

作者： Po liu Yang liu Chang-Neng Ke wei-shan li Yue-Ming liu Shi Xu Department of Burn and Plastic Surgery Shenzhen Longhua District Central HospitalShenzhen 518110Guangdong ProvinceChina

BACKGROUND Management of chronic refractory wounds is one of the toughest clinical challenges for *** of poor blood supply,less tissue coverage,and easy exposure,the lower leg is a common site for chronic refractory *** current therapeutic regimens often lead to prolonged hospital stay and higher healthcare *** growth factor(CGF)is a novel blood extract that contains various growth factors,platelets,and fibrins to promote wound healing ***,there has been little research reported on the treatment of lower extremity wounds with *** SUMMARY A 37-year-old man,without any past medical history,presented an ulcerated chronic wound on his right lower *** skin defect exhibited clear boundaries,with a size of 2.0 cm×3.5 *** depth of wound was up to the layer of deep *** aureus was detected by bacterial *** final diagnosis was right lower extremity ulcers with ***,silver sulfadiazine,and mupirocin cream were applied to control the *** gel was prepared from the patient’s blood sample,and was used to cover the wound after thorough *** skin wound was successfully healed after three times of CGF *** CGF displays an excellent wound healing promoting effect in patients with lowerextremity chronic refractory wounds.

关键词： Concentrated growth factors Chronic refractory wounds Lower extremity Ulcer Wound healing Case report

Synthesis and antiviral activities of novel 1,4-pentadien-3-one derivatives bearing an emodin moiety

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Chinese Chemical Letters 2016年第6期27卷 948-952页

作者： Jian Wu Yun-Ying Zhu Yong-Hui Zhao wei-li shan De-Yu Hu Ji-Xiang Chen Deng-Yue liu Xiang-Yang li Song Yang State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of Education Research and Development Center for Fine Chemicals Guizhou University School of Chemical Engineering Guizhou Institute of Technology Institute for the Control of Agrochemicals Ministry of Agriculture

A series of 1,5-diaryl-1,4-pentadien-3-one derivatives bearing an emodin group were designed and synthesized by the combination of natural products. The antiviral activities against tobacco mosaic virus（TMV） and cucumber mosaic virus（CMV） in vivo were evaluated. Some of the derivatives displayed promising curative effect and protective activity against TMV. Compound D5 showed appreciable curative bioactivity on TMV approximately of 50% at 306.2 mg/m L, which was superior to ningnanmycin（409.3 mg/m L）.

关键词： 1 4-Pentadien-3-one derivatives Emodin Synthesis Antiviral activity

Acute and chronic excitotoxicity in ischemic stroke and late-onset Alzheimer's disease

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Neural Regeneration Research 2025年第7期20卷 1981-1988页

作者： shan Ping Yu Emily Choi Michael QJiang ling wei Department of Anesthesiology Emory University School of MedicineAtlantaGAUSA Center for Visual and Neurocognitive Rehabilitation Atlanta Veterans Affairs Medical CenterDecaturGAUSA

Stroke and Alzheimer's disease are common neurological disorders and often occur in the same *** comorbidity of the two neurological disorders represents a grave health threat to older *** review presents a brief background of the development of novel concepts and their clinical *** activity of glutamatergic N-methyl-D-aspartate receptors and N-methyl-D-aspartate receptor-mediated Ca^(2+)influx is critical for neuronal *** ischemic insult induces prompt and excessive glutamate release and drastic increases of intracellular Ca^(2+)mainly via N-methyl-D-aspartate receptors,particularly of those at the extrasynaptic *** Ca^(2+)-evoked neuronal cell death in the ischemic core is dominated by necrosis within a few hours and days known as acute ***,mild but sustained Ca^(2+)increases under neurodegenerative conditions such as in the distant penumbra of the ischemic brain and early stages of Alzheimer's disease are not immediately toxic,but gradually set off deteriorating Ca^(2+)-dependent signals and neuronal cell loss mostly because of activation of programmed cell death *** on the Ca^(2+)hypothesis of Alzheimer's disease and recent advances,this Ca^(2+)-activated“silent”degenerative excitotoxicity evolves from years to decades and is recognized as a unique slow and chronic *** N-methyl-D-aspartate receptor subunit GluN3A,primarily at the extrasynaptic site,serves as a gatekeeper for the N-methyl-D-aspartate receptor activity and is neuroprotective against both acute and chronic *** stroke and Alzheimer's disease,therefore,share an N-methyl-D-aspartate receptor-and Ca^(2+)-mediated mechanism,although with much different time *** is thus proposed that early interventions to control Ca^(2+)homeostasis at the preclinical stage are pivotal for individuals who are susceptible to sporadic late-onset Alzheimer's disease and Alzheimer's disease-related

关键词： Ca^(2+)hypothesis cognitive deficits hyperactivity late-onset Alzheimer's disease neurodegeneration N-methyl-D-aspartate receptors N-methyl-D-aspartate receptor subunits pathogenesis preventive treatment

钼酸盐的电化学行为及其对甲醇氧化的催化作用

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电源技术 2001年第2期25卷 72-74,83页

作者：田立朋李伟善华南师范大学化学系广东广州510631

用循环伏安和线性电位扫描法研究了硫酸溶液中多晶铂电极上Na2 MoO4 的电化学行为及其对甲醇氧化的催化作用。结果表明 ,Na2 MoO4 在 3 .7mol/LH2 SO4 溶液中 ,一步还原生成不稳定的有色产物钼青铜 (HxMoO3) ,还原峰电位为0 .0V(相对于... 详细信息

用循环伏安和线性电位扫描法研究了硫酸溶液中多晶铂电极上Na2 MoO4 的电化学行为及其对甲醇氧化的催化作用。结果表明 ,Na2 MoO4 在 3 .7mol/LH2 SO4 溶液中 ,一步还原生成不稳定的有色产物钼青铜 (HxMoO3) ,还原峰电位为0 .0V(相对于饱和甘汞电极 ) ,并有一对应氧化峰。多晶铂电极因钼酸盐的还原与氧化而得以修饰。这种修饰电极对甲醇的氧化有很强的催化作用。修饰多晶铂电极上甲醇的氧化电流远大于未修饰多晶铂上的氧化电流。在 1mol/LCH3OH + 0 .0 5mol/LNa2 MoO4 + 3 .7mol/LH2 SO4 溶液中 ,正向扫描电位超过还原钼酸盐氧化峰电位后 ,氧化电流大于相应甲醇和钼酸盐硫酸溶液中氧化电流和的 2倍以上。甲醇在未修饰多晶铂电极上由于吸附一氧化碳中毒引起的电流下降现象 ,也因还原钼酸盐的修饰作用而消失。

关键词：甲醇氧化催化多晶铂电极钼酸盐电化学行为燃料电池

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锂离子电池凝胶聚合物隔膜的研究进展

物理化学学报 2017年第8期33卷 1533-1547页

作者：廖友好李伟善华南师范大学化学与环境学院广州510006 电化学储能材料与技术教育部工程研究中心广东省动力与储能电池材料工程技术研究中心广东省高能动力与储能电池有机高分子功能材料工程实验室广东高校电化学储能与发电技术重点实验室广州市动力与储能电池材料与器件产业技术创新平台广州510006

作为锂离子电池重要组分,隔膜由多孔聚烯烃高分子材料组成;电解质体系由有机碳酸酯和六氟磷酸锂混合组成,虽具有高离子电导率,但因液态碳酸酯的易燃特性给锂离子电池带来了安全隐患。利用能够将液态电解质体系凝胶化的聚合物制备得到的... 详细信息

作为锂离子电池重要组分,隔膜由多孔聚烯烃高分子材料组成;电解质体系由有机碳酸酯和六氟磷酸锂混合组成,虽具有高离子电导率,但因液态碳酸酯的易燃特性给锂离子电池带来了安全隐患。利用能够将液态电解质体系凝胶化的聚合物制备得到的凝胶聚合物隔膜,结合了液态电解质体系高电导率和固态电解质高安全性的优点。凝胶聚合物隔膜的研究从简单微孔凝胶聚合物隔膜开始,经历了引入少量纳米无机颗粒的掺杂凝胶聚合物隔膜,到引入大量纳米颗粒的凝胶陶瓷隔膜的发展历程。本文详细介绍这三种类型凝胶聚合物隔膜的物理化学特性,最后展望凝胶聚合物隔膜的发展趋势。

关键词：锂离子电池微孔凝胶聚合物隔膜掺杂凝胶聚合物隔膜凝胶陶瓷隔膜

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多孔阵列TiO_2/Ti的光电催化性能

物理化学学报 2009年第6期25卷 1173-1178页

作者：雷建飞李伟善华南师范大学化学与环境学院广州510006 广东高校电化学储能与发电技术重点实验室广州510006

以聚苯乙烯微球(PS)阵列为模板,溶胶浸渍法制得负载于钛基的多孔阵列二氧化钛材料TiO2/Ti,用SEM和XRD分析其表面形貌和晶相组成.将此多孔材料用作电极,研究其间歇光照开路电位谱和光电流谱,催化降解甲基橙以及催化析氢反应等光电催化性... 详细信息

以聚苯乙烯微球(PS)阵列为模板,溶胶浸渍法制得负载于钛基的多孔阵列二氧化钛材料TiO2/Ti,用SEM和XRD分析其表面形貌和晶相组成.将此多孔材料用作电极,研究其间歇光照开路电位谱和光电流谱,催化降解甲基橙以及催化析氢反应等光电催化性能.结果表明,在光电协同作用下多孔阵列TiO2/Ti具有很高的催化活性,2.5h内对甲基橙的降解率达到87.1%,5.0h高达97.9%,反应表观速率常数为0.77h-1;光照作用可以使析氢电位降低0.13V.

关键词：二氧化钛多孔阵列光催化降解甲基橙析氢

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中国入境旅游客源的文化分析

广西民族大学学报（哲学社会科学版） 2007年第1期29卷 140-144页

作者：李伟山广西民族大学管理学院

从文化学角度分析指出中国入境旅游客源市场呈现出随文化距离的增大而递减的总体特征;港澳台同胞、外国游客因文化背景的差异及其与中国内地文化关系的不同,在旅游需求结构上表现出“同中求异”与“异中求同”的文化特征。指出旅游市场... 详细信息

从文化学角度分析指出中国入境旅游客源市场呈现出随文化距离的增大而递减的总体特征;港澳台同胞、外国游客因文化背景的差异及其与中国内地文化关系的不同,在旅游需求结构上表现出“同中求异”与“异中求同”的文化特征。指出旅游市场细分和选择、旅游产品开发要以文化及其保护为基点,注意分销渠道的文化选择,以文化为媒进行宣传促销。

关键词：旅游文化学旅游市场国际旅游入境旅游