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The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

Food Science and Human Wellness 2022年第5期11卷 1240-1251页

作者： wei-kuang lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang Graduate Institute of Natural Products Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Graduate Institute of Integrated Medicine China Medical UniversityTaichung 404TaiwanChina Chinese Medicine Research and Development Center China Medical University HospitalTaichung 404TaiwanChina Department of Food Science and Nutrition Meiho UniversityPingtung 912TaiwanChina Department of Food Science National Chiayi UniversityChiayi City 600TaiwanChina Drug Development and Value Creation Research Center Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Medical Research Kaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Marine Biotechnology and Resources National Sun Yat-sen UniversityKaohsiung 804TaiwanChina

This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two *** protoplasts of *** were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm,and the protoplasts of *** were inactivated by UV radiation to invalidate their genome function,then they were fused under optimal conditions to get a fusion rate as(7.42±0.8)×10^(-6) fusants/mL;the new fusants were abbreviated as *** the other hand,when *** and *** were treated with heat and UV oppositely using similar experiments,the fusion rate was(9.70±0.68)×10^(-5) fusants/mL,and the new fusants were abbreviated as *** selected each of two best-growing fused colonies Ac-Cm-1,Ac-Cm-2,Cm-Ac-1,and Cm-Ac-2,together with parental *** and ***,and studied their morphology,growth antagonism tests,and genetic relationships by 18 S rRNA *** comparison with the initial cultures of 4 fusants,the yields of adenosine,biomass,cordycepic acid,cordycepin,total polysaccharide,and total triterpenoids were increased up 1.305-50.1563 times in the optimal medium *** gene stability tests,those of the four fusants and their outputs were stabilized within 10 generations.

关键词： Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite

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An improved imaging algorithm for spaceborne MAPs sliding spotlight SAR with high-resolution wide-swath capability

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Chinese Journal of Aeronautics 2015年第4期28卷 1178-1188页

作者： kuang Hui Chen Jie Yang wei Liu wei School of Electronic and Information Engineering Beihang University Electronic and Electrical Engineering Department University of Sheffield

Conventional synthetic aperture radar（SAR） systems cannot achieve both highresolution and wide-swath imaging *** problem can be mitigated by employing multiple-azimuth-phases（MAPs） technology for spaceborne sliding spotlight SAR ***, traditional imaging algorithms have met challenges to process the data accurately, due to range model error, MAPs data reconstruction problem, high-order cross-coupling phase error and variation of Doppler parameters along the azimuth ***, an improved imaging algorithm is proposed for solving the above ***, a modified hyperbolic range equation（MHRE） is proposed by introducing a cubic term into the traditional hyperbolic range equation（THRE）.And two curved orbit correction methods are derived based on the proposed range ***, a MAPs sliding spotlight data reconstruction method is introduced, which solves the spectral aliasing problem by a de-rotation ***, high-order cross-coupling phases and variation of Doppler parameters are analyzed and the corresponding compensation methods are *** results for point-target scene are provided to verify the effectiveness of the proposed algorithm.

关键词： High resolution Imaging Multiple-azimuth-phasesSliding spotlight Synthetic aperture radarWide swath

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Persistent alterations in gray matter in COVID-19 patients experiencing sleep disturbances:a 3-month longitudinal study

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Neural Regeneration Research 2025年第10期20卷 3013-3024页

作者： Kaixuan Zhou Gaoxiong Duan Ying Liu Bei Peng Xiaoyan Zhou Lixia Qin Lingyan Liang Yichen wei Qingping Zhang Xiaocheng Li Haixia Qin Yinqi lai Yian Lu Yan Zhang Jiazhu Huang Jinli Huang Yinfei Ouyang Bolin Bin Mingming Zhao Jun Liu Jianrong Yang Demao Deng Guangxi Key Laboratory of Special Biomedicine School of MedicineGuangxi UniversityNanningGuangxi Zhuang Autonomous RegionChina Department of Radiology the People’s Hospital of Guangxi Zhuang Autonomous RegionGuangxi Academy of Medical SciencesNanningGuangxi Zhuang Autonomous RegionChina Department of Sleep Medicine the People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxi Zhuang Autonomous RegionChina Department of Radiology The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Guangxi Clinical Research Center for Sleep Medicine the People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxi Zhuang Autonomous RegionChina

Sleep disturbances are among the most prevalent neuropsychiatric symptoms in individuals who have recovered from severe acute respiratory syndrome coronavirus 2 *** studies have demonstrated abnormal brain structures in patients with sleep disturbances who have recovered from coronavirus disease 2019(COVID-19).However,neuroimaging studies on sleep disturbances caused by COVID-19 are scarce,and existing studies have primarily focused on the long-term effects of the virus,with minimal acute phase *** a result,little is known about the pathophysiology of sleep disturbances in the acute phase of *** address this issue,we designed a longitudinal study to investigate whether alterations in brain structure occur during the acute phase of infection,and verified the results using 3-month follow-up data.A total of 26 COVID-19 patients with sleep disturbances(aged 51.5±13.57 years,8 women and 18 men),27 COVID-19 patients without sleep disturbances(aged 47.33±15.98 years,9 women and 18 men),and 31 age-and gender-matched healthy controls(aged 49.19±17.51 years,9 women and 22 men)were included in this *** COVID-19 patients with sleep disturbances were included in a longitudinal *** found that COVID-19 patients with sleep disturbances exhibited brain structural changes in almost all brain *** cortical thicknesses of the left pars opercularis and left precuneus were significantly negatively correlated with Pittsburgh Sleep Quality Index ***,we observed changes in the volume of the hippocampus and its subfield regions in COVID-19 patients compared with the healthy *** 3-month follow-up data revealed indices of altered cerebral structure(cortical thickness,cortical grey matter volume,and cortical surface area)in the frontal-parietal cortex compared with the baseline in COVID-19 patients with sleep *** findings indicate that the sleep disturbances patients had altered morphology in the cortical an

关键词： brain structure cortical gray matter volume cortical surface area cortical thickness hippocampus magnetic resonance imaging Pittsburgh Sleep Quality Index severe acute respiratory syndrome coronavirus 2 infections sleep disturbances sub-cortical volume

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Quantum parametric amplification of phonon-mediated magnon-spin interaction

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Science China(Physics,Mechanics & Astronomy) 2023年第11期66卷 92-105页

作者： Yan Wang Hui-lai Zhang Jin-Lei Wu Jie Song Kun Yang wei Qin Hui Jing Le-Man kuang School of Electronics and Information Zhengzhou University of Light IndustryZhengzhou450001China Academy for Quantum Science and Technology Zhengzhou University of Light IndustryZhengzhou450001China School of Physics and Microelectronics Zhengzhou UniversityZhengzhou450001China School of Physics Harbin Institute of TechnologyHarbin150001China Theoretical Quantum Physics Laboratory RIKEN Cluster for Pioneering ResearchWako-shiSaitama351-0198Japan Key Laboratory of Low-Dimensional Quantum Structures and Quantum Control of Ministry of Education Department of Physics and Synergetic Innovation Center for Quantum Effects and ApplicationsHunan Normal UniversityChangsha410081China

The recently developed hybrid magnonics provides new opportunities for advances in both the study of magnetism and the development of quantum information ***,engineering coherent quantum state transfer between magnons and specific information carriers,in particular,mechanical oscillators and solid-state spins,remains challenging due to the intrinsically weak interactions and the frequency mismatch between different ***,we show how to strongly couple the magnon modes in a nanomagnet to the quantized mechanical motion(phonons)of a micromechanical cantilever in a hybrid tripartite *** coherent and enhanced magnon-phonon coupling is engineered by introducing the quantum parametric amplification of the mechanical *** experimentally feasible parameters,we show that the mechanical parametric drive can be adjusted to drive the system into the strong-coupling regime and even the ultrastrong-coupling ***,we show the coherent state transfer between the nanomagnet and a nitrogen-vacancy center in the dispersive-coupling regime,with the magnon-spin interaction mediated by the virtually-excited squeezed *** amplified mechanical noise can hardly interrupt the coherent dynamics of the system even for low mechanical quality factors,which removes the requirement of applying additional engineered-reservoir *** work opens up prospects for developing novel quantum transducers,quantum memories and high-precision measurements.

关键词： mechanical parametric amplification strong-coupling regime hybrid quantum system

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Adaptive thermo-fluid moving boundary computations for interfacial dynamics

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Acta Mechanica Sinica 2012年第4期28卷 999-1021页

作者： Chih-kuang Kuan Jaeheon Sim wei Shyy University of Michigan Ann ArborMIUSA Hong Kong University of Science and Technology

In this study, we present adaptive moving boundary computation technique with parallel implementation on a distributed memory multi-processor system for large scale thermo-fluid and interfacial flow computations. The solver utilizes Eulerian-Lagrangian method to track moving （Lagrangian） interfaces explicitly on the stationary （Eulerian） Cartesian grid where the flow fields are computed. We address the domain decomposition strategies of Eulerian- Lagrangian method by illustrating its intricate complexity of the computation involved on two different spaces inter- actively and consequently, and then propose a trade-off ap- proach aiming for parallel scalability. Spatial domain decomposition is adopted for both Eulerian and Lagrangian do- main due to easy load balancing and data locality for mini- mum communication between processors. In addition, parallel cell-based unstructured adaptive mesh refinement （AMR） technique is implemented for the flexible local refinement and even-distributed computational workload among processors. Selected cases are presented to highlight the computa- tional capabilities, including Faraday type interfacial waves with capillary and gravitational forcing, flows around varied geometric configurations and induced by boundary conditions and/or body forces, and thermo-fluid dynamics with phase change. With the aid of the present techniques, large scale challenging moving boundary problems can be effectively addressed.

关键词： Moving boundary Eulerian-Lagrangian method Parallel adaptive mesh refinement

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珠江三角洲城市群留鸟多样性格局及其驱动机制

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中山大学学报（自然科学版）（中英文） 2025年第1期64卷 218-227页

作者：叶俊宏刘珍环杨帆魏莱周义广东省粤北岩溶区碳水耦合野外科学观测研究站/中山大学地理科学与规划学院广东广州510006

鸟类是反映城市生境和生物多样性变化的重要指标,城市鸟类多样性分布格局模拟与预测是当前城市生物多样性保护研究中重点关注的难题。珠江三角洲城市群经历了快速的城市化过程,景观变化显著,给城市生境和生物多样性保护带来了巨大挑战... 详细信息

鸟类是反映城市生境和生物多样性变化的重要指标,城市鸟类多样性分布格局模拟与预测是当前城市生物多样性保护研究中重点关注的难题。珠江三角洲城市群经历了快速的城市化过程,景观变化显著,给城市生境和生物多样性保护带来了巨大挑战。为解析城市鸟类多样性与城市生境之间的空间关联机制,构建“单物种分布预测-物种多样性聚类-多样性的影响归因”的空间综合途径,开展珠江三角洲城市群留鸟空间格局与形成机制研究。结果表明:1)珠江三角洲城市群的87种留鸟构成的多样性与适宜性具有高度的城-乡梯度分布模式;将留鸟的空间适宜性聚类可分为8类,总体上留鸟的中高适宜区占比小于30%。2)结构方程模型的解析可以发现,人类活动显著地正向影响了鸟类多样性的空间分布格局,影响系数介于0.28~1.94;而植被、气候、地形的正向或负向效应差异明显。研究可为城市生物多样性保护,调控鸟类多样性的生境影响因素,开展城市生态建设与管理提供科学依据。

关键词：城市生物多样性鸟类 MaxEnt模型结构方程模型

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Electrochemical hydrogen evolution efficiently boosted by interfacial charge redistribution in Ru/MoSe_(2) embedded mesoporous hollow carbon spheres

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Journal of Energy Chemistry 2023年第10期85卷 447-454,I0012页

作者： Yubin kuang wei Qiao Fulin Yang Ligang Feng School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002JiangsuChina

The strong metal-support interaction inducing combined effect plays a crucial role in the catalysis reaction. Herein, we revealed that the combined advantages of MoSe_(2), Ru, and hollow carbon spheres in the form of Ru nanoparticles(NPs) anchored on a two-dimensionally ordered MoSe_(2) nanosheet-embedded mesoporous hollow carbon spheres surface(Ru/MoSe_(2)@MHCS) for the largely boosted hydrogen evolution reaction(HER) performance. The combined advantages from the conductive support, oxyphilic MoSe_(2), and Ru active sites imparted a strong synergistic effect and charge redistribution in the Ru periphery which induced high catalytic activity, stability, and kinetics for HER. Specifically, the obtained Ru/MoSe_(2)@MHCS required a small overpotential of 25.5 and 38.4 mV to drive the kinetic current density of 10 mA cm^(-2)both in acid and alkaline media, respectively, which was comparable to that of the Pt/C catalyst. Experimental and theoretical results demonstrated that the charge transfer from MoSe_(2) to Ru NPs enriched the electronic density of Ru sites and thus facilitated hydrogen adsorption and water dissociation. The current work showed the significant interfacial engineering in Ru-based catalysts development and catalysis promotion effect understanding via the metal-support interaction.

关键词： Hydrogen evolution reaction Ruthenium Electrocatalyst MoSe_(2) Mesoporous hollow carbon spheres

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User centric dynamic web information visualization

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Science China(Information Sciences) 2013年第5期56卷 36-49页

作者： SALEHEEN Shibli lai wei Faculty of Information and Communication Technologies Swinburne University of TechnologyHawthorn 3122VictoriaAustralia

Visualization of information after retrieval from the World Wide Web is a very challenging task because of the huge amount of data in *** on rules,information is retrieved through mining techniques and then visualized in different *** web-graph is one way for users to visualize web data and its *** because of its huge size,the web-graph lacks simplicity for end *** clustering plays a very important role in reducing the complexity of the web graph by making it compact and simple,existing clustering techniques are insufficient to provide personalized visualization for ***,these clustering techniques end up showing the same clustered graph to every user,despite their varying *** work aims to make information visualization personalized based on user interests;to provide a good first impression of web information to end users;to make web navigation easier;and to create scope for further ***,we develop a system for clustering which first considers user interests and then visualizes the clustered web graph to make the information more useful to the end *** this paper,we present some experimental examples that show the improvements achieved by our approach over existing ones.

关键词： information retrieval user profile clustering personalization visualization

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Cauda equina syndrome with urinary retention as a postoperative complication of lumbar spine surgery:A case report

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World Journal of Clinical Cases 2025年第10期13卷 40-45页

作者： Kai-Wu Yang wei-Hong lai Da-wei Huang Department of Urology Chiayi Christian HospitalChiayi 600566Taiwan Department of Neurosurgery Chiayi Christian HospitalChiayi 600566Taiwan

BACKGROUND Cauda equina syndrome(CES)is characterized by a group of symptoms that may be caused by inflammation,spinal cord compression,venous congestion,or *** syndrome is commonly an indication for surgical intervention but has not been determined as a postoperative complication following surgery for lumbar spine *** SUMMARY To report the case of a 54-year-old male patient who had CES following spinal surgery,with no obvious compression lesions found during re-exploration,suggesting that vascular insufficiency may have contributed to the ***,a series of urodynamic studies on bladder recovery patterns in such complications have also been *** Postoperative CES requires urgent imaging and exploration to rule out compression;noncompressive cases,including vascular insufficiency may performed conservative management.

关键词： Cauda equina syndrome Lumbar spine surgery Urinary retention Urodynamic study Postoperative complication Case report

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Experimental and numerical study of tip injection in a subsonic axial flow compressor

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Chinese Journal of Aeronautics 2017年第3期30卷 907-917页

作者： wei WANG Wuli CHU Haoguang ZHANG Haiyang kuang School of Power and Energy Northwestern Polytechnical University Xi'an 710072 China Faculty of Water Resources and Hydroelectric Engineering Xi'an University of Technology Xi'an 710048 China Collaborative Innovation Center of Advanced Aero-Engine Beijing 100083 China

Parametric study of tip injection was implemented experimentally on a subsonic axial flow compressor to understand the underlying flow mechanisms of stability improvement of the compressor with discrete tip *** throat height varied from 2 to 6 times the height of rotor tip clearance,and circumferential coverage percentage ranged from 8.3% to 25% of the *** pressure fluctuations over the rotor tip were measured with fast-response pressure ***-passage time-accurate simulations were also carried out to help us understand the flow *** combinations of tip injection with traditional casing treatments were experimentally studied to generate an engineering-acceptable method of compressor stall *** results indicate that the maximum stability improvement is achieved when injectors are choked despite their different *** effect of circumferential coverage percentage on compressor stability depends on the value of injector throat height for un-choked injectors,and vice *** blockage in the blade passage is greatly reduced by the choked injectors,which is the primary reason for stability *** accomplishment of blockage diminishment is maintained in the circumferential direction with the unsteady effect of tip injection,which manifests as a hysteresis between the recovery of tip blockage and the recovery of tip leakage *** unsteady effect is primarily responsible for the effectiveness of tip injection with a partial circumferential *** injection cannot enhance the stability of the rotor with axial slots significantly,but it can improve the stability of the rotor with circumferential grooves *** combined structure of tip injection with circumferential grooves is an alternative for engineering application.

关键词： Axial flow compressor Casing treatments Compressor stability Tip blockage Tip injection Tip leakage vortex

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