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Gene heterogeneity of hepatitis B virus isolates from patients with severe hepatitis B

Hepatobiliary & Pancreatic diseases International 2005年第4期4卷 530-534页

作者： wei wu, yu chen, bing ruan and lan-juan li hangzhou, china department of infectious diseases, first affiliated hospital, zhejiang university school of medicine, key laboratory of infectious diseases of health ministry of china, hangzhou 310003, china department of infectious diseases First Affiliated Hospital Zhejiang University School of Medicine Key Laboratory of Infectious Diseases of Health Ministry of China Hangzhou 310003 China.

BACKGROUND: The pathogenesis of severe hepatitis B remains unknown. Reports have indicated that hepatitis B virus (HBV) mutations are important factors in the pathogenesis of this disease. This study was to investigate the genetic heterogeneity of HBV strains from serum samples of patients with fulminant hepatitis B. METHODS: Full-length HBV genomes from 4 patients with severe hepatitis B were cloned and sequenced to observe mutations in every open reading-frame ( ORF). Serum samples of another 25 patients with severe hepatitis B, 30 patients with chronic hepatitis B, and 25 HBV carriers were collected for sequencing and comparison of mutations in preS2, preC and core promoter regions. RESULTS: Of 4 HBV full-length genome sequences, 3 had a G to A mutation at nucleotide A1896 in the preC region and 1 had double mutations of T1762-A1764 in the core promoter region. The 4 sequences showed mutations in the known B or T cell epitopes of the preS2 and C regions. For the other 3 groups, more mutations were seen in the preS2 region in the HBV isolates from the patients with severe hepatitis B than those from the patients with chronic hepatitis B and HBV carriers (P <0.01). There was a significant difference of mutations in the T cell epitope region of preS2 between the patients with severe hepatitis B and those with chronic hepatitis B or HBV carriers (P <0.01). In the preC and core promoter regions, the mutation frequencies of T1653 and C1753 were 48.0% and 24.0% respectively in the patients with severe hepatitis B, but none of these mutations were observed in the patients with chronic hepatitis B group or HBV carriers (P <0.01). The mutation frequency of T1762-A1764 was 76.0% in the patients with severe hepatitis B, 40.0% in the patients with chronic hepatitis B (P <0. 01) , and 16. 0% in the HBV carriers ( P < 0. 01). There was a significant difference in A1896 mutation between the patients with severe hepatitis B and the patients with chronic hepatitis B (P < 0. 05 ) o

关键词： hepatitis B virus severe hepatitis virus genome gene heterogeneity preS2 preC core promoter

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Hepatitis C virus infection and biological falsepositive syphilis test:a single-center experience

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Hepatobiliary & Pancreatic diseases International 2011年第4期10卷 399-402页

作者： wei-Fang Zhu,Shui-Ying Lei and lan-juan li State key laboratory for Diagnosis and Treatment of infectious diseases key laboratory of infectious diseases,zhejiang Province department of infectious diseases,first affiliated hospital,zhejiang university school of medicine,hangzhou 310003,china State key laboratory for Diagnosis and Treatment of infectious diseases key laboratory of infectious diseases Zhejiang Province department of infectious diseases First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Rapid plasma regain positive and/or treponema pallidum hemagglutination negative [RPR(+)/TPHA(-)] results were designated as biologic false-positive (BFP).There are limited data about BFP reactions against syphilis in patients with hepatitis C virus (HCV) *** study aimed to determine the prevalence of BFP reactions for syphilis in patients with HCV infection in a large sample and assess the relationship between BFP reactions and HCV ***:A total of 2656 patients with positive anti-HCV and 5600 healthy control subjects were enrolled in this *** C serology was determined by a second generation EliSA test for HCV *** serology was determined by the RPR *** subjects with reactive RPR positive underwent the TPHA *** and laboratory data were collected by trained ***:Among 2656 patients,111 (4.2%) had a reactive RPR *** the 111 patients who were subjected to reactive RPR test,30 (27.0%) showed HCV(+)/RPR(+).Of 5600 healthy controls,80 (1.4%) had a reactive RPR *** (17.5%) controls with HCV(-)/RPR(+) had a non-reactive TPHA *** represented 1.1% of all HCV-positive and 0.3% of all HCV-negative subjects (Plis was observed in elderly HCV-seropositive ***-HCV positive group had a higher prevalence of *** eosinophil abnormality was compared between the patients and controls (66.7% vs 21.4%,P=0.0043).No significant results were observed in antinuclear antibodies,antiphospholipid and complement (C3,C4) (P>0.05).CONCLUSIONS:The data of this study demonstrate that HCV infection is associated with a false-positive RPR *** this study BFPs were significantly more common in HCV positive patients compared to HCV-negative *** abnormality can be considered as a predictor for *** BFPs must be considered in assessing the frequency of syphilis in a

关键词： hepatitis C virus biological false-positive syphilis

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Safety and efficacy of omalizumab for antihistamine-resistant chronic urticaria in children:a case series and literature review

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World Journal of Pediatrics 2024年第3期20卷 294-298页

作者： wei-Fang Zhu chen Wang Jian-Jun Qiao lan-juan li department of Dermatology First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China State key laboratory for Diagnosis and Treatment of infectious diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Chronic urticaria(CU)is a common skin disease characterized by the occurrence of wheals,with or without angioedema,lasting more than 6 *** prevalence of CU is about 0.7%[1],but higher in children,with an incidence of 1.38%[1].The occurrence of severe pruritus and unpredictable wheals may severely impact patients'quality of life,cause psychological problems,including sleep disorders and depression,and lead to economic burden due to limitation on daily life and work[2,3].

关键词： edema prevalence children

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Effects of Salvia miltiorrhiza on intestinal microflora in rats with ischemia/reperfusion liver injury

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Hepatobiliary & Pancreatic diseases International 2005年第2期4卷 274-280页

作者： Hui-Chun Xing, lan-juan li, Kai-Jin Xu, Tian Shen, yun-Bo chen, yu chen, Su-Zhen Fu, Ji-Fang Sheng, Chun-Lei chen, Jian-Guo Wang, Dong Yan, Fang-wei Dai and Xiao-Ying Sha hangzhou, china key laboratory of infectious diseases, ministry of Public health of china, and Institute of infectious diseases, department of infectious diseases, first affiliated hospital, zhejiang university school of medicine, hangzhou 310003, china zhejiang Academy of Medical Sciences, hangzhou 310012, china department of infectious diseases First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China.

BACKGROUND: Hepatic ischemia/reperfusion injury may induce intestinal microflora imbalance. Salvia miltiorrhiza is effective in promoting blood circulation and counteracting peroxidation in tissues. The aim of the present study was to determine the effects of Salvia miltiorrhiza on intestinal mi- croflora, endotoxemia, and bacterial translocation in rats with hepatic I/R injury. METHODS: Sprague-Dawley rats in specific pathogen free grade were divided into 3 groups: group I(n =6) for sham operation: groups ( n = 7) for liver ische- mia for 20 minutes and reperfusion for 22 hours. Group was also pretreated with 4 ml/day of Salvia miltiorrhiza solu- tion (250 mg/kg) by daily gavage for 7 days. The levels of serum alanine aminotransferase (ALT), aspartate amino- transferase (AST), malondialdehyde ( MDA) and supero- xide dismutase ( SOD ) in liver tissues, serum endotoxin, intestinal bacterial counts, intestinal mucosal histology and bacterial translocation were studied. RESULTS: The levels of ALT, AST, plasma endotoxin and MDA in liver tissues were decreased more markedly in group (57.57 ± 18.08 U/L, 147.57 ±40.84 U/L, 0.42 ± 0.144 EU/ml and 0. 52 ±0.19 nmol/mg-prot respectively) in group 295.9±216.92 U/L, 0.80± 0.262 EU/ml and 0.72±0.12 nmol/mg-prot; P liver SOD activity was increased more sig- nificantly in group (318.47±64.62 U/mg-prot) than in group U/mg-prot, Pliorated in group as compared with group CONCLUSIONS: Salviae miltiorrhiza could partially restore intestinal microflora balance, improve intestinal mucosal integrity, and reduce bacterial translocation and plasma en- dotoxin in rats with hepatic is

关键词： Salviae miltiorrhiza hepatic ischemia/reperfusion microflora endotoxin bacterial translocation

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High frequency of thrombocytopenia in patients with acute-on-chronic liver failure treated with linezolid

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Hepatobiliary & Pancreatic diseases International 2015年第3期14卷 287-292页

作者： Yi-Min Zhang wei yu Ning Zhou Jian-Zhou li li-chen Xu Zhong-Yang Xie Ying-Feng Lu lan-juan li State key laboratory for Diagnosis and Treatment of infectious diseases Department of Infectious Diseases the First Affiliated Hospital Zhejiang University School of Medicine Collaborative Innovation Center for Diagnosis and Treatment of infectious diseases

BACKGROUND： linezolid is an effective antibiotic reagent for Gram-positive bacterial infection; its most common side effect is thrombocytopenia. However, the incidence of throm- bocytopenia in patients with acute-on-chronic liver failure （ACLF） who underwent linezolid therapy was unclear. The present study was to evaluate the incidence of thrombocyto- penia in ACLF and non-ACLF patients treated with linezolid and the risk factors of thrombocytopenia in these patients.

关键词： acute-on-chronic liver failure linezolid thrombocytopenia

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai wujun Cai Yijian Cai Yizhou Cai yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang yue Chang Zhe Chang Xinyuan Chang wei Chao Auttakit Chatrabhuti Yimin Che yuzhi Che Bin chen Danping chen Fuqing chen Fusan chen Gang chen Guoming chen Hua-Xing chen Huirun chen Jinhui chen Ji-yuan chen Kai chen Mali chen Mingjun chen Mingshui chen Ning chen Shanhong chen Shanzhen chen Shao-Long chen Shaomin chen Shiqiang chen Tianlu chen wei chen Xiang chen Xiaoyu chen Xin chen Xun chen Xurong chen Ye chen Ying chen yukai chen Zelin chen Zilin chen Gang chen Boping chen Chunhui chen Hok Chuen cheng Huajie cheng Shan cheng Tongguang cheng yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi ling-yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH university of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui university HefeiAnhui Applied Physics department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu university Institute of ScienceVaranasi Bandirma Onyedi Eylul university Bandirma Beihang university Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal university Beijing Brown university ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad university Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo university Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central china Normal university WuhanHubei Central South university ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan china academy of engineering physics MianyangSichuan china building materials academy Beijing china Center of Advanced Science and Technology Beijing china Institute of Atomic Energy Beijing china Jiliang university HangzhouZhejiang china Nuclear Instrument CO. LTDBeijing china university of Geosciences Beijing china university of Geosciences WuhanHubei china university of Mining and Technology XuzhouJiangsu china West Normal university NanchongSichuan Chongqing university Department of PhysicsChongqing Chulalongkorn university Bangkok City university of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell university IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu university DalianLiaoning Dalian university of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East china university Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by china,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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Effects of plasma exchange combined with continuous renal replacement therapy on acute fatty liver of pregnancy

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Hepatobiliary & Pancreatic diseases International 2014年第2期13卷 179-183页

作者： cheng-Bo yu Jia-Jia chen wei-Bo Du Ping chen Jian-Rong Huang yue-Mei chen Hong-Cui Cao lan-juan li State key laboratory for Diagnosis and Treatment of infectious diseases the First Affiliated HospitalZhejiang University School of Medicine Collaborative Innovation Center for Diagnosis and Treatment of infectious diseases

BACKGROUND: Acute fatty liver of pregnancy (AFLP) in the third trimester or early postpartum period can lead to fatal liver damage. Its traditional therapy is not very effective in facilitating hepatic recovery. The safety and effect of plasma exchange (PE)in combination with continuous renal replacement therapy(CRRT) (PE+CRRT) for AFLP still needs ***: Five AFLP patients with hepatic encephalopathy and renal failure were subjected to PE+CRRT in our department from 2007 to 2012. Their symptoms, physical signs and results were observed, and all relevant laboratory tests were compared before and after PE+***: All the 5 patients were well tolerated to the therapy. Four of them responded to the treatment and showed improvement in clinical symptoms/signs and laboratory results and they were cured and discharged home after the treatment One patient succeeded in bridging to transplantation for slowing down hepatic failure and its complications process after2 treatment sessions. Intensive care unit stay and hospital stay were 9.4 (range 5-18) and 25.0 days (range 11-42), ***: PE+CRRT is safe and effective and should be used immediately at the onset of hepatic encephalopathy and/or renal failure in patients with AFLP.

关键词： plasma exchange continuous renal replacement therapy acute fatty liver pregnancy liver failure

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Immunological effects of a 10-μg dose of domestic hepatitis B vaccine in adults

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2012年第11期13卷 948-954页

作者： Jing-jing REN Xue-wei DAI Zheng-gang JIANG ling-zhi SHEN Yong-di chen Qian li Wen REN Ying liU Jun YAO lan-juan li State key laboratory for Diagnosis and Treatment of infectious Disease Key Laboratory of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China department of Epidemiology and Statistics School of Medicine Ningbo University Ningbo 315000 China zhejiang Center for Disease Control and Prevention Hangzhou 310003 China department of Family medicine the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Objective:To evaluate the immunological effects of three types of domestic 10-μg/dose hepatitis B vaccines in adults compared with a foreign vaccine, and to provide scientific evidence in support of adult hepatitis B vaccination. Methods:Adults from five counties (Deqing, Changxing, Nanxun, wuxing, Anji) in Huzhou City, Shaoxing County and Tongxiang County, zhejiang Province, china were selected. Blood samples were taken to assess serum HBsAg, anti-HBs, and anti-HBc using a chemiluminescence immunoassay. Adults, aged 16 to 49 years and who were anti-HBs negative at baseline, received hepatitis B immunizations at 0, 1, and 6 months. Anti-HBs levels were assessed one month after the third and final vaccination. Results:A total of 1 872 adults were immunized and the average positive rate was 89.5%. Four types of hepatitis B vaccine were used, including three from Chinese companies (Shenzhen Kangtai, Dalian High-Tech, and North china Pharmaceutical) and one from a UK company (GlaxoS-mithKline). Their seroconversion rates were 81.67%, 95.05%, 89.64%, and 86.81%, respectively. There was a significant difference between the anti-HBs positive conversion rates of the four types (P<0.005) but the seroconversion rates among the different vaccines were not significantly different (χ 2 =2.123, P=0.145). The average anti-HBs geometric mean titers (GMTs) of non-immune adults immunized with each of the four vaccines were 177.28, 473.23, 246.13, and 332.20 mIU/ml, respectively. There were no sta- tistically significant differences in the GMTs between the three types of domestic vaccine and the foreign vaccine (t= 1.575, P=0.116). Conclusions:Domestic recombinant hepatitis B vaccines can achieve immunization effects comparable to those of a foreign vaccine.

关键词： Hepatitis B Vaccines Immunization Geometric mean titer

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Plasma exchange-centered artificial liver support system in hepatitis B virus-related acute-onchronic liver failure:a nationwide prospective multicenter study in china

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Hepatobiliary & Pancreatic diseases International 2016年第3期15卷 275-281页

作者： Jia-Jia chen Jian-Rong Huang Qian Yang Xiao-wei Xu Xiao-li liu Shao-Rui Hao Hui-Fen Wang Tao Han Jing Zhang Jian-He Gan Zhi-liang Gao yu-Ming Wang Shu-Mei lin Qing Xie chen Pan lan-juan li State key laboratory for Diagnosis and Treatment of infectious diseases and Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesFirst Affiliated HospitalZhejiang University School of Medicine liver Failure Therapy and Research Center 302 Hospital of People’s Liberation Army department of Hepatology Third Central Clinical College of Tianjin Medical University Artificial liver Center You’an HospitalCapital Medical University department of infectious diseases First Affiliated Hospital of Suzhou University department of infectious diseases Third Affiliated Hospital of Sun Yat-Sen University Institute of infectious diseases Southwest HospitalThird Military Medical University department of infectious diseases First Affiliated Hospital of Xi’an Jiaotong University department of infectious diseases Ruijin HospitalShanghai Jiaotong University School of Medicine department of infectious diseases Infectious Diseases Hospital Affiliated to Fujian Medical University

BACKGROUND： Plasma exchange （PE）-centered artificial liver support system reduced the high mortality rate of hepa titis B virus （HBV）-related acute-on-chronic liver failure （ACLF）. But the data were diverse in different medical centers. The present prospective nationwide study was to evaluate the effects of PE on patients with HBV-ACLF at different stages.

关键词： liver failure artificial liver support plasma exchange acute-on-chronic liver failure

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Accuracy of pipeline blood glucose monitoring in patients with severe liver injury undergoing artificial liver support system treatment

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Hepatobiliary & Pancreatic diseases International 2019年第5期18卷 484-487页

作者： Hua-Fen Zhang Zhong-Yang Xie Xiao-Xi Ouyang Xiao-wei Xu Xiao-Yan Wang Jian-Rong Huang lan-juan li State key laboratory for Diagnosis and Treatment of infectious diseases The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China department of infectious diseases The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Collaborative Innovation Center for Diagnosis and Treatment of infectious diseases The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Severe deterioration of liver function in patients can be characterized by coagulation disorders, jaundice, hepatic encephalopathy, ascites, and other symptoms. Severe liver injury can develop as acute liver failure, ... 详细信息

关键词： pipeline blood glucose monitoring patients liver injury

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