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Involvement of sulfur assimilation in the low β subunit content of soybean seed storage protein revealed by comparative transcriptome analysis

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The crop Journal 2019年第4期7卷 504-515页

作者： Xi Zhang Ruixin Xu wei Hu Wan Wang Dezhi Han Fan Zhang Yongzhe Gu Yong Guo Jun Wang lijuan qiu College of Agriculture Yangtze University Jingzhou 434025 Hubei China national key facility for gene resources and genetic improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China Soybean Research Center Heihe Institute Heilongjiang Academy of Agricultural Sciences Heihe 100142 Helongjiang China

The β subunit of soybean [Glycine max (L.) Merr.] seed storage protein is of great significance in sulfur-containing amino acid balance and soybean processing properties. The objective of this study was to elucidate the relationship between the β subunit and sulfur-containing amino acid composition, and the potential regulatory mechanism. The β subunit was independently accumulated in comparison with other major subunits (α/α′, acidic, basic, and A3) during seed filling, and a low level of β subunit content (BSC) was formed during the accumulation process. In low-BSC mature seeds, crude protein, oil content, and fatty acid composition were not changed, but sulfur-containing amino acids (Cys + Met) in the low- BSC seeds increased significantly (by 31.5%), suggesting that an internal regulatory mechanism within seed might be responsible for the rebalance of seed protein composition and that sulfur assimilation might be deeply involved in β subunit accumulation. Transcriptomic analysis revealed that genes involved in anabolism of cysteine, methionine, and glutathione were up-regulated but those involved in the catabolism of these compounds were down-regulated, suggesting a relationship between the elevation of methionine and glutathione and low BSC. Our study sheds light on seed composition in low BSC lines and on the potential molecular regulatory mechanism of β subunit accumulation, broadening our understanding of soybean seed protein synthesis and its regulation.

关键词： Soybean β subunit Transcriptome analysis Sulfur-containing amino acids Sulfur assimilation

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genetic variation in a heat shock transcription factor modulates cold tolerance in maize

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Molecular Plant 2024年第9期17卷 1423-1438页

作者： Lei Gao Lingling Pan Yiting Shi Rong Zeng Minze Li Zhuoyang Li Xuan Zhang Xiaoming Zhao Xinru Gong wei Huang Xiaohong Yang Jinsheng Lai Jianru Zuo Zhizhong Gong Xiqing Wang weiwei Jin Zhaobin Dong Shuhua Yang State key Laboratory of Plant Environmental Resilience Frontiers Science Center for Molecular Design BreedingCollege of Biological SciencesCenter for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China State key Laboratory of Maize Bio-breeding National Maize Improvement CenterFrontiers Science Center for Molecular Design BreedingDepartment of Plant Geneticsand BreedingChinaAgricultural UniversityBeijingChina State key Laboratory of Plant Genomics and national Plant gene Research Center Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China

Understanding how maize(Zea mays)responds to cold stress is crucial for facilitating breeding programs of cold-tolerant *** extensive utilization of the genome-wide association study(GWAs)approach for exploring favorable natural alleles associated with maize cold tolerance,few studies have successfully identified candidate genes that contribute to maize cold *** this study,we used a diverse panel of inbred maize lines collected from different germplasm sources to perform a GWAS on var-iations in the relative injured area of maize true leaves during cold stress-a trait very closely correlated with maize cold *** identified HsF21,which encodes a B-class heat shock transcription factor(HSF)that positively regulates cold tolerance at both the seedling and germination *** varia-tions in the promoter of the cold-tolerant HSF21Hap1 allele led to increased HSF21 expression under cold stress by inhibiting binding of the basic leucine zipper bziP68 transcription factor,a negative regulator of cold *** integrating transcriptome deep sequencing,DNA affinity purification sequencing,and targeted lipidomic analysis,we revealed the function of HsF21 in regulating lipid metabolism homeo-stasis to modulate cold tolerance in *** addition,we found that HsF21 confers maize cold tolerance without incurring yield ***,this study establishes HsF21 as a key regulator that en-hances cold tolerance in maize,providing valuable genetic resources for breeding of cold-tolerant maize varieties.

关键词： heat shock factor HSF21 cold tolerance natural variation bZiP68 lipid metabolism maize

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Genomics-based plant germplasm research(GPGR)

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The crop Journal 2017年第2期5卷 166-174页

作者： Jizeng Jia Hongjie Li Xueyong Zhang Zichao Li lijuan qiu key Laboratory of crop Germplasm & Biotechnology Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural SciencesThe National Key Facility for Crop Gene Resources and Genetic ImprovementNFCRGI beijing key Laboratory of crop genetic improvement Key Laboratory of Crop Heterosis and UtilizationMinistry of EducationChina Agricultural University

Plant germplasm underpins much of crop genetic improvement. Millions of germplasm accessions have been collected and conserved ex situ and/or in situ, and the major challenge is now how to exploit and utilize this abundant resource. Genomics-based plant germplasm research （GPGR） or ＂Cenoplasmics＂ is a novel cross-disciplinary research field that seeks to apply the principles and techniques of genomics to germplasm research. We describe in this paper the concept, strategy, and approach behind GPGR, and summarize current progress in the areas of the definition and construction of core collections, enhancement of germplasm with core collections, and gene discovery from core collections. GPGR is opening a new era in germplasm research. The contribution, progress and achievements of GPGR in the future are predicted.

关键词： Plant germplasm Genomics Genomics-based plant germplasm research(GPGR) Genoplasmics

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大豆新品种黑农84品质性状的环境稳定性分析

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大豆科学 2024年第1期43卷 1-12页

作者：王婉刘鑫磊孙君明栾晓燕邱丽娟中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程/农业部北京大豆生物学重点实验室北京100081 黑龙江省农业科学院大豆研究所黑龙江哈尔滨150086

为明确黑农84的品质稳定性,为新品种的推广应用提供依据,本研究分析了黑农84不同年份、不同地点种植的品质性状变异。结果显示:黑农84是具备高蛋白、低脂肪、高亚麻酸、高异黄酮、高蔗糖和低水苏糖特性的优质功能型品种。在检测的27个... 详细信息

为明确黑农84的品质稳定性,为新品种的推广应用提供依据,本研究分析了黑农84不同年份、不同地点种植的品质性状变异。结果显示:黑农84是具备高蛋白、低脂肪、高亚麻酸、高异黄酮、高蔗糖和低水苏糖特性的优质功能型品种。在检测的27个品质性状中,16个性状(蛋白质和脂肪含量,蛋脂总量,棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸含量,维生素E含量,水溶性糖,γ-维生素E,δ-维生素E,果糖、葡萄糖、蔗糖和水苏糖含量)的变异系数小于10%,较为稳定。两年的遗传多样性指数变异范围分别为1.08~1.98和1.07~1.73,平均值分别为1.62和1.59,其中α-大豆苷的遗传多样性指数最高(1.98和1.73),棉子糖的遗传多样性指数均最小(1.08和1.07)。黑农84品质性状聚类均可以分为两类,第一类中蛋白质和α-维生素E含量较高,脂肪和异黄酮含量较低;第二类中大豆苷、m-大豆苷、α-大豆苷和m-染料木苷含量较低。黑农84优质性状的环境稳定性为该品种的遗传育种和加工应用等提供了理论依据。

关键词：黑农84 品质性状遗传多样性指数聚类分析环境稳定性

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Development and utilization of a new chemically-induced soybean library with a high mutation density

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Journal of Integrative Plant Biology 2017年第1期59卷 60-74页

作者： Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin lijuan Zhang Yinghui Li Yulong Ren wei he Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang lijuan qiu national key facility for gene resources and genetic improvement/key Laboratory of crop Germplasm Utilization Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural Science Soybean Research institute Heilongjiang Academy of Agricultural Sciences College of Agriculture Yangtze University

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was *** 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（ChlI1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

关键词： soybean mutant yellow chemically golden phenotype genomics mutation Glycine chloroplast

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QTL mapping and transcriptome analysis identify candidate genes influencing water–nitrogen interaction in maize

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The crop Journal 2023年第6期11卷 1872-1883页

作者： Kunhui he Yakun Zhang wei Ren Pengyun Chen Jianchao Liu Guohua Mi Fanjun Chen Qingchun Pan State key Laboratory of Nutrient Use and Management College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China College of Agronomy Northwest A&F UniversityYangling 712100ShaanxiChina Sanya institute of china agricultural University Sanya 572000HainanChina

Water and nitrogen fertilization are the key factors limiting maize *** genetic basis of interactions between maize genotype,water,and nitrogen is unclear.A recombinant inbred line(RIL)maize population was evaluated for seven yield and five agronomic traits under four water and nitrogen conditions:water stress and low nitrogen,water stress and high nitrogen,well-watered and low nitrogen,and well-watered and high *** eight,six,and six traits varied in response to genotype–water interactions,genotype–nitrogen interactions,and genotype–water–nitrogen *** a linkage map consisting of 896 single-nucleotide polymorphism markers and multipleenvironmental quantitative-trait locus(QTL)mapping,we identified 31 QTL,including 12 for genotype–water–nitrogen interaction,across the four treatments.A set of 8060 genes were differentially expressed among *** genetic analysis,gene co-expression,and functional annotation revealed two candidate genes controlling genotype–water–nitrogen interactions,affecting both leaf width and grain *** involved in abscisic acid biosynthesis and bZIP,NAC,and WRKY transcription factors participated in maize response to water and nitrogen *** results represent a step toward understanding the genetic regulatory network of maize that responds to water and nitrogen stress and provide a theoretical basis for the genetic improvement of both water-and nitrogen-use efficiency.

关键词： Quantitative trait loci Water Nitrogen Interaction Maize

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Differentially expressed miRNAs in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A

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Journal of Integrative Agriculture 2020年第7期19卷 1731-1742页

作者： DONG Yun WANG Yi JIN Feng-wei XING lijuan FANG Yan ZHANG Zheng-ying ZOU Jun-jie WANG Lei XU Miao-yun crop Research institute Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China national key facility for crop gene resources and genetic improvementBiotechnology Research institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Gansu Provincial key Lab of Aridland crop sciences/Gansu agricultural University Lanzhou 730070.P.R.China

In Brassica napus L.(rapeseed),complete genic male sterility(GMS)plays an important role in the utilization of *** microRNAs(miRNAs)play essential regulatory roles during bud development,knowledge of how GMS is regulated by miRNAs in rapeseed is rather *** this study,we obtained a novel recessive GMS system,*** sterile line CN12A has defects in tapetal diferentiation and *** sequencing was employed to examine the expression of miRNAs in the buds of CN12A and the fertile line *** identifed 85 known miRNAs and 120 novel miRNAs that were expressed during rapeseed anther *** comparing the expression levels of miRNAs between CN12A and CN12B,19 and 18 known miRNAs were found to be difrentially expressed in 0.5--1.0 mm buds and in 2.5--3.0 mm buds,*** these,the expression levels of 14 miRNAs were higher and the levels of 23 miRNAs were lower in CN12A compared with *** predicted target genes of these iferentially expressed miRNAS encode protein kinases,F-box domain-containing proteins,MADS-box family proteins,SBP-box gene family members,HD ZIP proteins,floral homeotic protein APETAL A 2(AP2)。and nuclear factor Y,subunit *** targets have previously been reported to be involved in pollen development and male sterility,suggesting that miRNAs might act as regulators of GMS in rapeseed ***,RT-qPCR data suggest that one of the dffrentially expressed miRNAs,bna-miR159,plays a role in tapetal differentiation by regulating the expression of transcription factor BnMYB101 and participates in tapetal degradation and infuences callose degradation by manipulating the expression of *** findings contibute to our understanding of the roles of miRNAs during anther development and the occurrence of GMS in rapeseed.

关键词： Brassica napus L. genic male sterility miRNAs targets molecular regulation network

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Functional analyses of an E3 ligase gene AIP2 from wheat in Arabidopsis revealed its roles in seed germination and pre‐harvest sprouting

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Journal of Integrative Plant Biology 2014年第5期56卷 480-491页

作者： Dong-Yao Gao Zhao-Shi Xu Yi he Yong-wei Sun You-Zhi Ma Lan-Qin Xia institute of crop sciences/The national key facility for crop gene resources and genetic improvement the Chinese Academy of Agricultural Sciences (CAAS) agricultural and Sideline Base Unit 65426 of the People’s Liberation Army

Pre‐harvest sprouting（PHS） seriously affects wheat yield and quality of the grain. ABI3 is a key factor in the activation of seed development and repression of germination in Arabidopsis. An ABI3‐interacting protein（AIP2） could polyubiquitinate ABI3, impair seed dormancy and promote seed germination in Arabidopsis. In this study,two wheat AIP2 genes, TaAIP2A and TaAIP2B, were *** localization assay and yeast two‐hybrid analysis revealed that TaAIP2A and TaAIP2B may function through interaction with wheat Viviporous‐1（TaVp1）. The transcripts TaAIP2A and TaAIP2B were more abundant in wheat PHS susceptible cultivars than that of resistant ones, and decreased gradually following seed development. Expression of TaAIP2A and TaAIP2B in Arabidopsis aip2‐1 mutant lines resulted in earlier flowering, promotion of seed germination,and reduced ABA sensitivity, respectively, somehow mimicking the phenotype of the wild type, with TaAIP2B having a stronger role in these aspects. Furthermore, the expression ofupstream genes ABI1 and ABI2 were upregulated, whereas that of downstream genes ABI3 and ABI5 were downregulated in both TaAIP2A and TaAIP2B complemented lines upon ABA treatment. These results suggested that wheat AIP2s could negatively regulate the ABA signaling pathway and play important roles in seed germination, and thus wheat PHS resistance finally.

关键词： ABA responsiveness AIP2 Arabidopsis E3 ligase functional analysis pre‐harvest sprouting Triticum aestivum L

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Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis

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chinese science Bulletin 2009年第19期54卷 3536-3545页

作者： ZHOU GuoAn GUAN RongXia LI YingHui CHANG RuZhen qiu lijuan national key facility for crop gene resources and genetic improvement (NFCRI)/key Lab of Germplasm & Biotechnology (MOA) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

关键词：植物耐盐基因工程盐胁迫离子分布植物组织 GmNHX2

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基于国内外大豆品种比较的中国大豆品种改良路径探索

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大豆科学 2024年第4期43卷 395-401页

作者：郭勇姜思琦于莉莉徐江源洪慧龙邱丽娟中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室/农作物基因资源与遗传改良国家重大科学工程/农业农村部北京大豆生物学重点实验室北京100081

我国的大豆生产落后于世界大豆主产国,从品种角度看,存在着单产水平低、油分含量低等问题。本文对主产国大豆品种研发应用情况以及美国大豆品种发展趋势进行分析,明确了美国在大豆种质的资源研究、育种技术研发、品种特性鉴定以及环境... 详细信息

我国的大豆生产落后于世界大豆主产国,从品种角度看,存在着单产水平低、油分含量低等问题。本文对主产国大豆品种研发应用情况以及美国大豆品种发展趋势进行分析,明确了美国在大豆种质的资源研究、育种技术研发、品种特性鉴定以及环境因素研究等方面对于大豆品种研发的促进作用。通过对中美大豆品种特性的全面比较分析,明确我国大豆品种在单产、含油量、种植密度等方面的差距,提出了快速提升我国大豆品种产量和品质的路径,即通过对高油大豆资源进行表型精准鉴定,研发全基因组选择、转基因等高效育种技术,聚合产量和其他优异性状,创制优异大豆种质,设计培育高油高产突破性品种,以期为中国大豆品种改良研究提供参考。

关键词：大豆品种改良高油高产路径

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