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Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families

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science china(Life sciences) 2008年第8期51卷 687-692页

作者： WANG YuePing1,2, CheN XiongTing2 & qiu lijuan1 1 national key facility for crop gene resources and genetic improvement, key Laboratory of crop Germplasm & Biotechnology(MOA)/institute of crop science, chinese academy of agricultural sciences, beijing 10008, china 2 institute of Tropical Biotechnology, chinese academy of Tropical agricultural sciences, Haikou 571101, china 1. national key facility for crop gene resources and genetic improvement Key Laboratory of Crop Germplasm & Biotechnology (MOA)/Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 10008 China 2. institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China

Trypsin inhibitors have been found in various animals, plants and *** were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors(BBI) and Kunitz in-hibitors(KTI).The different BBI genes from wild soybean(***) and cultivated soybean(***) formed a multigene *** constructed a cDNA library of cultivar 'SuiNong 14' seed at the R7 growth stage using the SMART *** contigs or singletons were highly homologous to soy-bean protease *** of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, *** analyses showed there were novel allelic varia-tions among the 4 BBI members in SuiNong *** on the comparison of soybean seed cDNA li-braries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in *** analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common pro-genitor.

关键词： soybean trypsin inhibitor Bowman-Birk proteinase inhibitor Kunitz trypsin inhibitor allelic variation

Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses

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science china(Life sciences) 2010年第11期53卷 1315-1321页

作者： GAO Fei1,2, SU Qi1, FAN YunLiu1& WANG Lei1 1Biotechnology Research institute/national key facility for crop gene resources and genetic improvement, chinese academy of agricultural science, beijing 100081, china 2College of Life and Environmental sciences, Minzu University of china, beijing 100081, china 1. Biotechnology Research institute/national key facility for crop gene resources and genetic improvement Chinese Academy of Agricultural Science Beijing 100081 China2. College of Life and Environmental Sciences Minzu University of China Beijing 100081 China

The protein kinase AtMPK3,a component of the MAP kinase cascade,plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3 is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3 gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3 promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3 promoter, and introduced the intact and truncated promoter sequences fused to the β-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between-188 and-62 upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3 promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli.

关键词： Arabidopsis thaliana AtMPK3 promoter stress

MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

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science Bulletin 2012年第Z2期 3804-3810页

作者： LUO ZhongQin,JIN LongGuo & qiu lijuan * The national key facility for crop gene resources and genetic improvement(NFCRI),institute of crop science,chinese academy of agricultural sciences,beijing 100081,china

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5’RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词： soybean miR1511 miR1511 * GmRPL4a

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

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science china(Life sciences) 2008年第3期51卷 280-285页

作者： LI Ang1,2, CheN LiangLiang1,3, REN HaiYun3, WANG XueChen2, ZHANG HaiWen1,4 & HUANG Rong- Feng1,4 1 Biotechnology Research institute, chinese academy of agricultural sciences, beijing 100081, china 2 national Laboratory of Plant Physiology and Biochemistry, College of Biological sciences, china agricultural University, beijing 100094, china 3 College of Life sciences, beijing Normal University, beijing 100875, china 4 national key facility of crop gene resources and genetic improvement, beijing 100081, china Biotechnology Research institute Chinese Academy of Agricultural Sciences Beijing 100081 China national Laboratory of Plant Physiology Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China College of Life sciences Beijing Normal University Beijing 100875 China national key facility of crop gene resources genetic improvement Beijing 100081 China

In rice, the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further de- termine the crucial sequences responsible for MeJA induction, we generated a series of deletion pro- moters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is po- sitioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (?1127), which is distinct from the essential region containing ERE (?562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

关键词： OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves

Proteomics Investigation of Wheat-Stripe Rust Fungus Interactions

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Proteomics Investigation of Wheat-Stripe Rust Fungus Interac...

第三届全国植物蛋白质组学大会

作者： Pan Yinghong institute of crop science,chinese academy of agricultural sciences(CAAS) /The national key facility for crop gene resources and genetic improvement(NFCRI)

Stripe rust of wheat is one of the most important diseases of wheat worldwide,but the mechanism of interaction between wheat and stripe rust fungus is *** using proteomics technologies,we identified some different pro...

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QTL for grain yield and yield parameters in winter wheat(Triticum aestivum L.)

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湖南农业大学学报（自然科学版） 2007年第S1期33卷 86-91页

作者： LIN Hai,GUO Hui-jun,ZHANG Xiu-ying,WANG Rui-xia,XIAO Shi-he(The national key facility for crop gene resources and genetic improvement,institute of crop science,12 Zhongguancun South Street,beijing,100081,P.R)

Field trials with a set of 108 doubled haploid lines(DHs) derived from a cross between the chinese winter wheat ***9613 and H1488 were run at beijing(china).Phenotypic data were recorded for major agronomic yield traits,*** weight per ear,grain number per ear and thousand grain weight(Tgw) in two field trials at *** on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci(QTL) was carried out for yield and yield parameters using the composite interval mapping(CIM) approach.A total of 14 QTL were detected for these traits across two *** of these QTL located on chromosomes 1A and 2B,respectively,exhibited pleiotropic *** showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate("pyramide") favorable alleles at different loci.

关键词： Doubled haploids Quantitative trait loci(QTL) Grain yield wheat(Triticum aestivum L.)

Diversity of waxy gene in chinese Rice Germplasm Resource

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Diversity of waxy Gene in Chinese Rice Germplasm Resource

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Chen You-Tao,Qiao wei-Hua,Yang Qin-Wen~* The national key facility for crop gene resources and genetic improvement institute of crop science,chinese academy of agricultural sciences,beijing 100081 *Author for correspondence

Waxy gene;Diversity;SNP;Granule-bound starch synthase;Domestication;Rice

关键词： Waxy gene Diversity SNP Granule-bound starch synthase Domestication Rice

Mining the candidate genes related to SCN based on the pedigree analysis

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Mining the candidate genes related to SCN based on the pedig...

第十三届全国植物基因组学大会

作者： Shan-shan Zhang,Yin-hui Li,Li-juan qiu~* The national key facility for crop gene resources and genetic improvement(NFCRI), institute of crop science,chinese academy of agricultural sciences,beijing 100081,china

Soybean cyst nematode(SCN) was one of the most destructive pests for soybean production which could easily cause significant agricultural *** discovering the new SCN related genes or markers,17 cultivars from the pedi...

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Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558

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Journal of Integrative Agriculture 2013年第3期12卷 398-405页

作者： ZheNG Yong-sheng MA Xiao-gang CHI De-zhao GAO Ai-nong LI Li-hui LIU wei-hua national key facility for crop gene resources and genetic improvement/institute of crop sciences Chinese Academy of Agricultural Sciences Qinghai academy of Agriculture and Forestry

Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis （2-DE） and nano- scale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins （DEPs）, which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-1ike proteins and proliferating cell nuclear antigen （PCNA）, involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidation- reduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.

关键词： wheat spike-development inhibition 2-DE differentially expressed proteins