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Gene Transfer to Dendritic Cells Induced a Protective Immunity against Melanoma

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Cellular & Molecular Immunology 2005年第4期2卷 281-288页

作者： Pat Metharom Kay A.O. Ellem Ming q. wei queensland Institute of Medical Research Herston Department of Medicine University of Queensland Prince Charles Hospital Brisbane Australia

Lentiviral vectors have shown promises for efficient gene transfer to dividing as well as nondividing cells. In this study, we explored lentiviral vector-mediated, the entire mTRP-2 gene transfer and expression in dendritic cells （DCs）. Adoptive transfer of DCs-expressing mTRP-2 （DC-HR＇CmT2） into C57BL/6 mouse was also assessed. Dendritic cells were harvested from bone marrow and functional DCs were proved by allogeneic mixed lymphocyte reaction. Lentiviral vectors were produced by transient transfection of 293T cells. Transduction of DCs was proved by marker gene expression and PCR and RT-PCR amplification. Implantation of the transduced DCs, depletion of immune cells as well as the survival of the mice after tumour challenge were investigated. High efficiency of gene transfer into mature DCs was achieved. The high level expression of the functional antigen （TRP-2） and induction of protective immunity by adoptive transfer of TRP-2 gene modified DCs were demonstrated. In vivo study showed a complete protection of mice from further melanoma cell challenge. In comparison, only 83% of mice survived when mTRP-2 peptide-pulsed DCs were administered, suggesting the generation of specific protection. Together, these results demonstrated the usefulness of this gene transfer to DC approach for immunotherapy of cancer and indicated that using tumour associated antigens （TAAs） for gene transfer may be potentially beneficial for the therapy of melanoma.

关键词： lentiviral vector gene transfer dendritic cell tumour antigen melanoma

Search for“Weapons of Mass Destruction”for Cancer-Immuno/Gene Therapy Comes of Age

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Cellular & Molecular Immunology 2005年第5期2卷 351-357页

作者： Ming q. wei Pat Metharom Kay A.O. Ellem Stefan Barth Department of Medicine University of Queensland Prince Charles Hospital Brisbane Australia queensland Institute of Medical Research Herston Brisbane Australia Fraunhofer IME. 1st floor A106 Forckenbeckstr. 652074 Aachen Germany Corresponding to： Dr. Ming q. wei Head Gene Therapy Unit Department of Medicine University of Queensland Prince Charles Hospital Brisbane Qld. 4032 Australia.

The complexity of a cancer, such as cell heterogeneity, and the existence of hypoxia, stromai cells and stem cells has so far prevented successful development and treatment of patients suffering from the later stages of cancers. At present, the use of conventional therapies, such as chemo/radio therapy is limited, and only therapies that are focused on utilizing the patient＇s immune response to combat against the disease appear to be the most reliable and promising. Two decades ago, cytokines were discovered to be able to activate the immune systems and mount an anti-tumour response. Then, dendritic cells were hailed as the most significant regulators of immunity and are employed in a variety of cancer management schemes. This review introduces current development in the field, focusing on combination of the components of ＇he rapidly growing fields of immunotherapy and gene transfer/therapy, providing useful and significant detailed information for readers of cellular and molecular immunology.

关键词： cancer immunotherapy gene therapy dendritic cell

PARAMETRIC STUDY FOR THE PREPARATION OF ALIGNED SINGLE-WALLED CARBON NANOTUBES BY ANODE-ARC DISCHARGE METHOD

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Acta Metallurgica Sinica(English Letters) 2005年第3期18卷 261-268页

作者： J.F.Dai q.Wang W.X.Li Z.q.wei G.J.Xu StateKeyLaboratoryofNewNonferrousMetalMaterials DepartmentofPhysicsLanzhouUniversityofTechnologyLanzhou730050China StateKeyLaboratoryofNewNonferrousMetalMaterials InstituteofMaterialsLanzhouUniversityofTechnologyLanzhou730050China

Well aligned quasi-straight single-walled carbon nanotubes (SWCNTs) and straight SWCNTs bundle have been prepared in large scale by anode-arc vaporization of gr aphite with metallic catalysts. Various parameters such as the catalyst preparat ion, the kinds and pressure of the buffer gases, the quantity of anode-arc curre nt intensity, and the method of purification have been examined. The influence o f these parameters on the deposited carbon yield is reported, together with obse rvations of the produced material. Improvement in synthetic techniques has resul ted in the optimal conditions for the production of large quantities of high qua lity SWCNTs in our semi-continuous synthesis method. The formation of carbon nan otubes (CNTs) was studied briefly in this paper. Owing to the magnetic pinching effect of arc current, the CNTs arrange in parallel lines along the arc current direction.

关键词： carbon nanotubes anode-arc preparation catalyst

PARTICLE SIZE AND MICROSTRUCTURE OF Ni NANOPOWDERS PREPARED BY ANODIC ARC PLASMA

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Acta Metallurgica Sinica(English Letters) 2005年第3期18卷 209-216页

作者： Z.q.wei H.X.qiao J.F.Dai W.J.Feng q.Wang P.X.Yan SchoolofSciences LanzhouUniversityofTechnologyLanzhou730050China CollegeofCivilEngineering LanzhouUniversityofTechnologyLanzhou730050China SchoolofPhysicalScienceandTechnology LanzhouUniversityLanzhou730000China

Pure Ni nanopowders were successfully prepared by the method of anodic arc disch arged plasma with homemade experimental apparatus. The particle size, mircostruc ture and morphology of the particles by this process were characterized via X-ra y powder diffraction (XRD), transmission electron microscopy (TEM) and the corre sponding selected area electron diffraction (SAED); The specific surface area an d pore parameters were investigated by multi-point full analysis of nitrogen ads orption-desorption isotherms at 77K by Brunauer- Emmett-Teller (BET) surface are a analyzer; The chemical composition were determined by X-ray energy dispersive spectrometry (XEDS) equipped in SEM and element analyze instrument. The experime nt results indicate that the samples by this method with high purity,the crystal structure of the particles is as same as the bulk material, is fcc structure, w ith average particle sizes about 47nm, ranging from 20 to 70nm, and distributed uniformly in spherical chain shapes, the specific surface areavis 14.23m2/g, po re volume of pore is 0.09cm3/g and average pore diameter is 23nm.

关键词： anodic arc plasma Ni nanopowder particle size structure composition.

NANOSIZE EFFECT IN GRAIN BOUNDARY MIGRATION OF COPPER

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Acta Metallurgica Sinica(English Letters) 2004年第1期17卷 11-15页

作者： L.Zhou X.q.wei N.G.Zhou D.G.Li School of Materials Science and Engineering Nanchang UniversityNanchang 330047ChinaSchool of Materials Science and EngineeringNanchang UniversityNanchang 330047ChinaSchool of Materials Science and EngineeringNanchang UniversityNanchang 330047ChinaCentral Iron and Steel Research InstituteBeijing 100083China

Molecular dynamics simulations of high temperature annealing of copper bicrystals have been carried out. The bicrystals have planar grain boundaries, and the gain size varies in nano range. An EAM (embedded atom method) potential of FS type is used for calculating the interatomic forces. The results show that in nanocrystalline copper, GB migration driven by inter-GB reaction can take place. A critical grain size is identified, below which the inter-GB reaction becomes strong enough to trigger GB motion, which accelerates rapidly and leads to annihilation of the grain boundaries. The critical size is found to be 16 atomic radii. A "through intermediate grain mechanism" is identified for the nano-grain boundary motion observed, which is never reported for GB migrations of conventional polycrystalline metals.

关键词： grain boundary migration nanocrystal copper

mRNA vaccines:a new era in vaccine development

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Oncology Research 2024年第10期32卷 1543-1564页

作者： SHUBHRA CHANDRA JENNIFER C.WILSON DAVID GOOD MING q.wei School of Pharmacy&Medical Sciences Gold Coast campusGriffith UniversityBrisbaneQLD-4222Australia Menzies Health Institute queensland(MHIq) Gold Coast CampusGriffith UniversityBrisbaneQLD-4215Australia School of Allied Health Australian Catholic UniversityBrisbaneQLD-4014Australia

The advent of RNA therapy,particularly through the development of mRNA cancer vaccines,has ushered in a new era in the field of *** article provides a concise overview of the key principles,recent advancements,and potential implications of mRNA cancer vaccines as a groundbreaking modality in cancer *** cancer vaccines represent a revolutionary approach to combatting cancer by leveraging the body’s innate immune *** vaccines are designed to deliver specific mRNA sequences encoding cancer-associated antigens,prompting the immune system to recognize and mount a targeted response against malignant *** personalized and adaptive nature of mRNA vaccines holds immense potential for addressing the heterogeneity of cancer and tailoring treatments to individual *** breakthroughs in the development of mRNA vaccines,exemplified by the success of COVID-19 vaccines,have accelerated their application in *** mRNA platform’s versatility allows for the rapid adaptation of vaccine candidates to various cancer types,presenting an agile and promising avenue for therapeutic *** trials of mRNA cancer vaccines have demonstrated encouraging results in terms of safety,immunogenicity,and *** candidates,such as BioNTech’s BNT111 and Moderna’s mRNA-4157,have exhibited promising outcomes in targeting melanoma and solid tumors,*** successes underscore the potential of mRNA vaccines to elicit robust and durable anti-cancer immune *** the field holds great promise,challenges such as manufacturing complexities and cost considerations need to be addressed for widespread *** development of scalable and cost-effective manufacturing processes,along with ongoing clinical research,will be pivotal in realizing the full potential of mRNA cancer ***,mRNA cancer vaccines represent a cutting-edge therapeutic approach that holds the promise of transforming cancer treatment.

关键词： Cancer immunotherapy Immune checkpoint Preventive&therapeutic vaccine Delivery system mRNA

EFFECTS OF Zn CONCENTRATION ON WETTABILITY OF Sn-Zn ALLOY ON Cu AND ON THE INTERFACIAL MICROSTRUCTURE BETWEEN Sn-Zn ALLOY AND Cu

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Acta Metallurgica Sinica(English Letters) 2006年第4期19卷 251-257页

作者： H.Z. Huang X.q. wei L. Zhou X.D. Liu G.L. Guo School of Materials Science and Engineering Nanchang University Nanchang 330047 China East China Institute of Technology Fuzhou 344000 China

The potential of using a hypoeutectic, instead of eutectic, Sn-Zn alloy as a lead-free solder has been discussed. The nonequilibrium melting behaviors of a series of Sn-Zn alloys were examined by differential thermal analysis. It was found that at a heating rate of 5℃/min, *** exhibited no melting range. Dipping and spreading tests were carried out to characterize the wettability of Sn-Zn alloys on Cu. Both tests exhibited that Sn-6.5Zn has significantly better wettability on Cu than Sn-9Zn. The reaction layers formed during the spreading tests were examined. When the Zn concentration fell between 2.5wt%-9wt%, two reaction layers were formed at the interface, a thick and flat Cu5Zn8 adjacent to Cu and a thin and irregular Cu-Zn-Sn layer adjacent to the alloy. Only a Cu0Sn5 layer was formed when the Zn concentration decreased to 0.5wt%. The total thickness of the reaction layer（s） between the alloy and Cu was found to increase linearly with the Zn concentration.

关键词： lead-free solder Sn-Zn alloy wettability interface intermetallic compound

Announcing Signal Transduction and Targeted Therapy

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Signal Transduction and Targeted Therapy 2016年第1期1卷 140-140页

作者： CM Croce K Zhang Y-q wei Institute of Genetics The Ohio State UniversityColumbusOHUSA Shiley Eye Institute Institute for Engineering in MedicineInstitute for Genomic MedicineUniversity of California San DiegoSan DiegoCAUSA State Key Laboratory of Biotherapy West China HospitalSichuan University/Collaborative Innovation Center of BiotherapySichuanChina

Since the concept of signal transduction was introduced in the 1970s,various signal transduction pathways and signaling molecules have been identified.A major driving force in this field is the potential for signaling... 详细信息

关键词： signal Signal driving

Acute and chronic excitotoxicity in ischemic stroke and late-onset Alzheimer's disease

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Neural Regeneration Research 2025年第7期20卷 1981-1988页

作者： Shan Ping Yu Emily Choi Michael qJiang Ling wei Department of Anesthesiology Emory University School of MedicineAtlantaGAUSA Center for Visual and Neurocognitive Rehabilitation Atlanta Veterans Affairs Medical CenterDecaturGAUSA

Stroke and Alzheimer's disease are common neurological disorders and often occur in the same *** comorbidity of the two neurological disorders represents a grave health threat to older *** review presents a brief background of the development of novel concepts and their clinical *** activity of glutamatergic N-methyl-D-aspartate receptors and N-methyl-D-aspartate receptor-mediated Ca^(2+)influx is critical for neuronal *** ischemic insult induces prompt and excessive glutamate release and drastic increases of intracellular Ca^(2+)mainly via N-methyl-D-aspartate receptors,particularly of those at the extrasynaptic *** Ca^(2+)-evoked neuronal cell death in the ischemic core is dominated by necrosis within a few hours and days known as acute ***,mild but sustained Ca^(2+)increases under neurodegenerative conditions such as in the distant penumbra of the ischemic brain and early stages of Alzheimer's disease are not immediately toxic,but gradually set off deteriorating Ca^(2+)-dependent signals and neuronal cell loss mostly because of activation of programmed cell death *** on the Ca^(2+)hypothesis of Alzheimer's disease and recent advances,this Ca^(2+)-activated“silent”degenerative excitotoxicity evolves from years to decades and is recognized as a unique slow and chronic *** N-methyl-D-aspartate receptor subunit GluN3A,primarily at the extrasynaptic site,serves as a gatekeeper for the N-methyl-D-aspartate receptor activity and is neuroprotective against both acute and chronic *** stroke and Alzheimer's disease,therefore,share an N-methyl-D-aspartate receptor-and Ca^(2+)-mediated mechanism,although with much different time *** is thus proposed that early interventions to control Ca^(2+)homeostasis at the preclinical stage are pivotal for individuals who are susceptible to sporadic late-onset Alzheimer's disease and Alzheimer's disease-related

关键词： Ca^(2+)hypothesis cognitive deficits hyperactivity late-onset Alzheimer's disease neurodegeneration N-methyl-D-aspartate receptors N-methyl-D-aspartate receptor subunits pathogenesis preventive treatment