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One-step cell biomanufacturing platform:porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo

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Neural Regeneration Research 2024年第2期19卷 458-464页

作者： Lin Feng Da Li Yao Tian Chengshun zhao Yun Sun xiaolong Kou Jun wu Liu Wang Qi Gu wei Li Jie Hao Baoyang Hu Yukai Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Institute for Stem Cell and Regenerative Medicine Chinese Academy of SciencesBeijingChina National Stem Cell Resource Center Chinese Academy of SciencesBeijingChina Savaid Medical School University of Chinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina

Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s *** of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s ***,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation ***,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor *** beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in ***,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell ***,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.

关键词： axonal integrity cell cryopreservation cellular environment cellular niche cell replacement therapy dopaminergic progenitors human pluripotent stem cell mechanical damage neuronal cell delivery Parkinson’s disease small-aperture gelatin microcarriers

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折光率仪快速测定盐渍土总盐含量的方法研究

中国建材科技 2024年第2期33卷 41-45页

作者：冯金凤李晓民赵静卓魏定邦魏强魏小龙武旭邵敏刚甘肃省交通规划勘察设计院股份有限公司甘肃兰州730030 兰州交通大学甘肃兰州730030

建筑面临的腐蚀现象中盐害问题最突出。为快速、准确测定总盐含量,研究了折光率仪检测盐渍土总盐含量的方法。选取甘肃河西地区58个土样为研究对象,制备了不同土水比浸出液,使用折光率仪测定浸出液的折光率。建立了土壤总盐含量与不同... 详细信息

建筑面临的腐蚀现象中盐害问题最突出。为快速、准确测定总盐含量,研究了折光率仪检测盐渍土总盐含量的方法。选取甘肃河西地区58个土样为研究对象,制备了不同土水比浸出液,使用折光率仪测定浸出液的折光率。建立了土壤总盐含量与不同土水比浸出液折光率之间的相关性曲线。结果表明,土壤总盐含量与不同土水比浸出液折光率之间呈显著正相关,其中,土壤总盐含量与土水比1:1浸出液折光率的相关性最紧密。同时,提出了一种通过测定浸出液折光率,由建立的相关性方程测定土壤总盐含量的方法。

关键词：总盐含量折光率浸出液相关性

One-year status of hepatic venous pressure gradient measurement from 85 hospitals in China

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Portal Hypertension & Cirrhosis 2024年第2期3卷 116-123页

作者： Jitao Wang Ting Cui Linpeng Zhang Zhiping Yan Lei Li Jinjun Chen Junhui Sun Hua Xiang He Zhu Hao wu Jiancuo Gengzang Feng Zhang wei wu Guohong Cao wuhua Guo Haibin Shi Hui Xue Guangjun Huang Qiang Yu Meng Niu Huiling Xiang Derun Kong wei Gou Xuefeng Luo Fuliang He Min Yuan wei Liu Yong Wang Hongjie Hu Xiaoli Zhu Tao Yu Qinxue Sun wei Qu Ting Lu Deqiang Ma Li Zhang Jun Ma Jun Yang Ketao Mu Xiaoliang Zhu Kai Xiong Huiguo Ding Shengjuan Yao Mingsheng Huang Fang Wang Zexin Wang Yong Huang Jianan Li Hongfeng Yi Birun Huang Zhongwei zhao Duiping Feng Yanming Lei Changlong Hou Wenbo Guo Shirong Liu Gaojun Teng Kangshun Zhu Fan wu Xiaogang Hu Wenfeng Zhang Shaoqi Yang Zhouchao Hu Pengfei Pang Shaowu Zhuang weidong Wang Qingliang Zhu Kunpeng Zhang Chengyu Liu Dongliang Li Chao Ma Hong Jiang Xingong zhao weixin Ren Zhiwei Wang Lei Yu Fuhuang Lin Chaoguang Yan Feng Wang Lei Yu Hui Huan Wenyong Shen Shaofei He Chengda Zhang Jinlun Bao Jiangtao Liu Jun wu Jianbo zhao Jian Zhang xiaolong Qi Hebei Provincial Key Laboratory of Cirrhosis&Portal Hypertension Xingtai People's HospitalXingtaiHebeiChina Center of Portal Hypertension Department of RadiologyZhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsuChina Department of Interventional Therapy Beijing Shijitan Hospital Affiliated to Capital Medical UniversityBeijingChina Department of Intervention Taiyuan Third People's HospitalTaiyuanShanxiChina Department of Intervention Zhongshan Hospital Affiliated to Fudan UniversityShanghaiChina Department of Interventional Medicine The First Hospital of Lanzhou UniversityLanzhouGansuChina Department of Infectious Diseases Nanfang Hospital of Southern Medical UniversityGuangzhouGuangdongChina Hepatobiliary and Pancreatic Intervention Center The First Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiangChina Interventional Vascular Surgery Hunan Provincial People's HospitalChangshaHunanChina Department of Intervention Sixth People's Hospital of ShenyangShenyangLiaoningChina Department of Gastroenterology West China Hospital of Sichuan UniversityChengduSichuanChina Department of Intervention The Fourth People's Hospital of Qinghai ProvinceXiningQinghaiChina Department of Gastroenterology Nanjing Drum Tower HospitalNanjingJiangsuChina Department of Gastroenterology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiangChina Department of Radiology Shulan(Hangzhou)HospitalHangzhouZhejiangChina Department of Intervention Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouFujianChina Department of Interventional Radiology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsuChina Department of Gastroenterology The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'anShaanxiChina Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandongChina Department of Interventional Radiology Fifth Medical Center

Aims:Surveys and research on the applications of the hepatic venous pressure gradient(HVPG)are important for understanding the current status and future development of this technology in *** article aimed to investigate the status of hepatic venous pressure gradient measurement in China in ***:We investigated the overall status of HVPG technology in China-including hospital distribution,hospital level,annual number of cases,catheters used,average cost,indications,and current challenges by using online *** counting the number and percentages of cases of these results,we hope to clarify the current status of HVPG measurements in ***:According to the survey,85 hospitals in China used HVPG technology in 2022 distributed across 29 provinces.A total of 4989 HVPG measurements were performed in all of the surveyed hospitals in 2022,of which 2813 cases(56.4%)were measured *** average cost of HVPG measurement was 5646.8±2327.9 *** the clinical teams who performed the measurements(sometimes multiple per hospital),94.3%(82/87)used the balloon method,and the majority of the teams(72.4%,63/87)used embolectomy ***:This survey clarified the clinical application status of HVPG in China and confirmed that some medical institutions in China have established a foundation for this *** is still necessary to continue promoting and popularizing this technology in the future.

关键词： application status hepatic vein pressure gradient questionnaire survey

原发性失眠患者奖赏网络关键核团在静息态fMRI功能连接的改变

维普期刊数据库评论

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海南医学院学报 2024年第16期30卷 1230-1235页

作者：孙曌冉彬艳阚丽娜黄禄宇吴琼王乙翔康晓娜李丹丹王晓彤王小龙靳丹阳沈巍海南医学院中医学院海南海口570100 海南医学院第一附属医院海南海口571199 中山市中医院广东中山528401

目的:利用功能性磁共振成像(fMRI)技术观察原发性患者奖赏网络区域中脑腹侧被盖(Ventral ttegmental area,VTA)以及伏隔核(Nucleus accumbens,NAcc)脑功能连接的改变。方法:28例原发性失眠比较26例健康对照组脑功能成像的改变,并分析中... 详细信息

目的:利用功能性磁共振成像(fMRI)技术观察原发性患者奖赏网络区域中脑腹侧被盖(Ventral ttegmental area,VTA)以及伏隔核(Nucleus accumbens,NAcc)脑功能连接的改变。方法:28例原发性失眠比较26例健康对照组脑功能成像的改变,并分析中脑腹侧被盖以及伏隔核功能连接的改变与失眠的情绪障碍障碍之间的关系。结果:比较原发性失眠患者对比健康对照组,VTA与NAcc全脑的功能连接均降低,与VTA-l功能连接增降低的脑区为双侧前扣带回、左侧眶额回,与VTA-r功能连接增降低的脑区为双侧前扣带回、左侧眶额回、右侧中央前回,与NAcc-l功能连接降低的脑区为双侧丘脑、辅助运动区,与NAcc-r功能连接降低的脑区为双侧丘脑、小脑。结论:睡眠障碍会显著降低奖赏网络中VTA与NAcc的功能连接,说明长期睡眠障碍可能影响在原发性失眠患者奖赏处理能力,导致失眠患者情感功能发生障碍。

关键词：功能性磁共振成像广泛性焦虑障碍脑功能前扣带回前额叶

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低温下苜蓿生理代谢及抗寒基因差异分析

中国饲料 2024年第11期 180-186页

作者：王晓龙杨曌柴华李莎莎徐艳霞吴玥高海娟王建丽王锐李伟王若丁张杨黑龙江省农业科学院畜牧兽医分院/黑龙江省牧草育种与种质资源利用工程技术研究中心黑龙江齐齐哈尔161005 黑龙江省农业科学院草业研究所黑龙江哈尔滨150086

本试验旨在筛选适宜黑龙江西部地区种植的耐寒苜蓿种质材料,探究低温胁迫下苜蓿幼苗的生理响应机制,为苜蓿耐寒种质资源鉴定提供理论依据。以4个苜蓿品种为试验材料,通过低温处理测定生理指标游离脯氨酸(Pro)、可溶性糖(SS)、丙二醛(MDA... 详细信息

本试验旨在筛选适宜黑龙江西部地区种植的耐寒苜蓿种质材料,探究低温胁迫下苜蓿幼苗的生理响应机制,为苜蓿耐寒种质资源鉴定提供理论依据。以4个苜蓿品种为试验材料,通过低温处理测定生理指标游离脯氨酸(Pro)、可溶性糖(SS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、相对电导率和基因NAC3、CAS18表达量,利用隶属函数评价筛选出耐寒苜蓿品种。结果表明:随低温胁迫程度加剧,供试苜蓿品种的Pro、SS、MDA和电导率呈现升高趋势,SOD和POD活性呈现先升后降的变化;重度胁迫时,肇东苜蓿的Pro、SS、SOD和POD均为最高,而电导率为最低;同时肇东苜蓿的NAC3、CAS18基因表达量显著高于其他品种(P皇后>420>赛迪。

关键词：紫花苜蓿幼苗期低温生理特性抗寒基因评价

汽轮机组TSI系统转速监控点调试和故障处理对策

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自动化应用 2024年第7期65卷 135-137页

作者：戚继厚陈伟吴晓龙王学军徐化冰厉飞赵勇伟华能山东如意(巴基斯坦)能源(私人)有限公司山东济南250000

针对现有汽轮机组TSI系统在转速监测与故障处理方面中存在的问题,如人工操作繁琐、转速信号丢失、转速测量不准确及缺乏自动化故障检测与报警等,阐述了TSI系统在汽轮机组中的作用与重要性,深入探讨TSI系统转速监控点调试,包括转速监控... 详细信息

针对现有汽轮机组TSI系统在转速监测与故障处理方面中存在的问题,如人工操作繁琐、转速信号丢失、转速测量不准确及缺乏自动化故障检测与报警等,阐述了TSI系统在汽轮机组中的作用与重要性,深入探讨TSI系统转速监控点调试,包括转速监控点的选择与布置、传感器选择与安装及自动化调试方法,在此基础上给出一些有效的转速监控点故障处理对策,以期提高汽轮机组的可靠性与运行效率。

关键词：汽轮机组 TSI系统转速监控点调试

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油莎豆快速繁殖体系构建研究

中国饲料 2024年第5期 122-128页

作者：王晓龙钟鹏杨曌柴华李莎莎徐艳霞吴玥高海娟王宏伟王建丽李伟王若丁孙蕊李莉张杨黑龙江省农业科学院畜牧兽医分院黑龙江齐齐哈尔161005 通辽市农牧科学研究所内蒙古通辽028000 黑龙江省农业科学院草业研究所黑龙江哈尔滨150086

为建立油莎豆(Cyperus esculentus)快速繁殖体系,本试验以黑油莎2号为试验材料,茎尖作为外植体,采用植物不同组织培养条件培养油莎豆无菌苗,以期筛选出最佳茎尖消毒方法、适宜茎尖分化培养基配方以及快速生根培养基配方。结果表明:随消... 详细信息

为建立油莎豆(Cyperus esculentus)快速繁殖体系,本试验以黑油莎2号为试验材料,茎尖作为外植体,采用植物不同组织培养条件培养油莎豆无菌苗,以期筛选出最佳茎尖消毒方法、适宜茎尖分化培养基配方以及快速生根培养基配方。结果表明:随消毒时间的增加,黑油莎2号茎尖污染率呈现降低的趋势,而存活率则呈现先升后降的变化;MS+0.9 mg/L 6-苄基腺膘呤(6-BA)+0.3 mg/L萘乙酸(NAA)处理时,黑油莎2号茎尖分化成苗数量显著高于其他处理(P <0.05),且茎尖分化成苗率最高,为62.67%;1/2MS+0.2 mg/L吲哚丁酸(IBA)处理时,黑油莎2号无菌苗生根数量(29.00)最高,显著高于其他处理(P <0.05),且根系粗壮,根长为0.9~3.0 cm。最后得出75%酒精30s+0.1%升汞4 min为黑油莎2号最佳茎尖消毒方法,MS+0.9 mg/L 6-BA+0.3 mg/L NAA为最佳茎尖分化培养基配方,MS+1.0 mg/L 6-BA和1/2MS+0.2 mg/L IBA分别是最佳快速繁殖和生根培养基配方。油莎豆快速繁殖体系构建为寒区高效繁育油莎豆优异种苗及种质资源创新提供依据。

关键词：油莎豆无菌苗茎尖分化快速繁殖生根

Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

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Frontiers of Medicine 2023年第2期17卷 240-262页

作者： Jing Feng Zheng zhao Yanfei wei zhaoshi Bao wei Zhang Fan wu Guanzhang Li Zhiyan Sun Yanli Tan Jiuyi Li Yunqiu Zhang Zejun Duan Xueling Qi Kai Yu Zhengmin Cong Junjie Yang Yaxin Wang Yingyu Sun Fuchou Tang Xiaodong Su Chuan Fang Tao Jiang xiaolong Fan Beijing Key Laboratory of Gene Resource and Molecular Development Laboratory of Neuroscience and Brain DevelopmentBeijing Normal UniversityBeijing 100875China Beijing Neurosurgical Institute Beijing 100070China Department of Neurosurgery Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Department of Pathology Affiliated Hospital of Hebei UniversityBaoding 071000China Gendya Biotechnology Ltd. Beijing 100176China Center of Growth Metabolism&Aging Key Laboratory of Bio-Resource and Eco-Environment of Ministry of EducationCollege of Life SciencesSichuan UniversityChengdu 610065China Department of Pathology Sanbo Brain HospitalCapital Medical UniversityBeijing 100093China Biodynamic Optical Imaging Center(BIOPIC) School of Life SciencesPeking UniversityBeijing 100871China Department of Neurosurgery Affiliated Hospital of Hebei UniversityBaoding 071000China Hebei Key Laboratory of Precise Diagnosis and Treatment of Glioma Baoding 071000China Chinese Glioma Genome Atlas Network(CGGA) Beijing 100070China College of Life Sciences Sichuan Normal UniversityChengdu 610101China

Detailed characterizations of genomic alterations have not identified subtype-specific vulnerabilities in adult gliomas. Mapping gliomas into developmental programs may uncover new vulnerabilities that are not strictly related to genomic alterations. After identifying conserved gene modules co-expressed with EGFR or PDGFRA (EM or PM), we recently proposed an EM/PM classification scheme for adult gliomas in a histological subtype- and grade-independent manner. By using cohorts of bulk samples, paired primary and recurrent samples, multi-region samples from the same glioma, single-cell RNA-seq samples, and clinical samples, we here demonstrate the temporal and spatial stability of the EM and PM subtypes. The EM and PM subtypes, which progress in a subtype-specific mode, are robustly maintained in paired longitudinal samples. Elevated activities of cell proliferation, genomic instability and microenvironment, rather than subtype switching, mark recurrent gliomas. Within individual gliomas, the EM/PM subtype was preserved across regions and single cells. Malignant cells in the EM and PM gliomas were correlated to neural stem cell and oligodendrocyte progenitor cell compartment, respectively. Thus, while genetic makeup may change during progression and/or within different tumor areas, adult gliomas evolve within a neurodevelopmental framework of the EM and PM molecular subtypes. The dysregulated developmental pathways embedded in these molecular subtypes may contain subtype-specific vulnerabilities.

关键词： glioma progression molecular classification EM PM subtyping intratumor heterogeneity

Catalytically active atomically thin cuprate with periodic Cu single sites

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National Science Review 2023年第1期10卷 226-235页

作者： Huimin Yang Shibo Xi Na Guo Mu Wang Lingmei Liu Pin Lyu xiaolong Yu Jing Li Haomin Xu Xiao Hai Zejun Li Xinzhe Li Tao Sun Xiaoxu zhao Yu Han wei Yu Jie wu Chun Zhang Honghan Fei Ming Joo Koh Jiong Lu Department of Chemistry National University of Singapore Institute of Chemical and Engineering Sciences Department of Physics National University of Singapore Advanced Membranes and Porous Materials Center King Abdullah University of Science and Technology SDU-ANU Joint Science College Shandong University School of Materials Science and Engineering Peking University Centre for Advanced 2D Materials and Graphene Research Centre National University of Singapore Department of Chemistry Tongji University

Rational design and synthesis of catalytically active two-dimensional(2D) materials with an abundance of atomically precise active sites in their basal planes remains a great ***,we report a ligand exchange strategy to exfoliate bulk [Cu4(OH)6] [O3S(CH2)4SO3] cuprate crystals into atomically thin 2D cuprate layers([Cu2(OH)3]+).The basal plane of 2D cuprate layers contains periodic arrays of accessible unsaturated Cu(Ⅱ) single sites(2D-CuSSs),which are found to promote efficient oxidative Chan-Lam *** me chanistic studies reveal that the reactions proceed via coordinatively unsaturated CuO4(Ⅰ) single sites with the formation of Cu(Ⅰ) species in the rate-limiting step,as corroborated by both operando experimental and theoretical *** robust stability of 2D-CuSSs in both batch and continuous flow reactions,coupled with their recyclability and good performance in complex molecule derivatization,render 2D-CuSSs attractive catalyst candidates for broad utility in fine chemical synthesis.

关键词： exfoliation nanosheet heterogeneous catalysis