One-step cell biomanufacturing platform:porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo

作     者：Lin Feng Da Li Yao Tian Chengshun Zhao Yun Sun Xiaolong Kou Jun Wu Liu Wang Qi Gu Wei Li Jie Hao Baoyang Hu Yukai Wang Lin Feng;Da Li;Yao Tian;Chengshun Zhao;Yun Sun;Xiaolong Kou;Jun Wu;Liu Wang;Qi Gu;Wei Li;Jie Hao;Baoyang Hu;Yukai Wang

作者机构：State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of ZoologyChinese Academy of SciencesBeijingChina Institute for Stem Cell and Regenerative MedicineChinese Academy of SciencesBeijingChina National Stem Cell Resource CenterChinese Academy of SciencesBeijingChina Savaid Medical SchoolUniversity of Chinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative MedicineBeijingChina State Key Laboratory of Membrane BiologyInstitute of ZoologyChinese Academy of SciencesBeijingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2024年第19卷第2期

页      面：458-464页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China,Nos.2017YFE0122900(to BH),2019YFA0110800(to WL),2019YFA0903802(to YW),2021YFA1101604(to LW),2018YFA0108502(to LF),and 2020YFA0804003(to JW) the National Natural Science Foundation of China,Nos.31621004(to WL,BH)and 31970821(to YW) CAS Project for Young Scientists in Basic Research,No.YSBR-041(to YW) Joint Funds of the National Natural Science Foundation of China,No.U21A20396(to BH) 

主　　题：axonal integrity cell cryopreservation cellular environment cellular niche cell replacement therapy dopaminergic progenitors human pluripotent stem cell mechanical damage neuronal cell delivery Parkinson’s disease small-aperture gelatin microcarriers 

摘      要：Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s *** of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s ***,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation ***,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor *** beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in ***,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell ***,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.