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Journal of Integrative Plant Biology 2014年第1期56卷 51-62页

作者： Caiping Cai Wenxue Ye Tianzhen Zhang wangzhen guo State Key Laboratory of Crop Genetics & Germplasm Enhancement Hybrid Cotton R & D Engineering Research Center Ministry of EducationNanjing Agricultural University

Exploring the elite al eles and germplasm acces-sions related to fiber quality traits wil accelerate the breeding of cotton for fiber quality improvement. In this study, 99 Gossypium hirsutum L. accessions with diverse origins were used to perform association analysis of fiber quality traits using 97 polymorphic microsatel ite marker primer pairs. A total of 107 significant marker-trait associations were detected for three fiber quality traits under three different environments, with 70 detected in two or three environments and 37 detected in only one environment. Among the 70 significant marker-trait associations, 52.86% were reported previously, implying that these are stable loci for target traits. Furthermore, we detected a large number of elite al eles associated simulta-neously with two or three traits. These elite al eles were mainly from accessions col ected in China, introduced to China from the United States, or rare al eles with a frequency of less than＆amp;nbsp;5%. No one cultivar contained more than half of the elite al eles, but 10 accessions were col ected from China and the two introduced from the United States did contain more than half of these al eles. Therefore, there is great potential for mining elite al eles from germplasm accessions for use in fiber quality improvement in modern cotton breeding.

关键词： Association mapping elite allele fiber quality populationstructure Upland cotton

Fine mapping of the red plant gene R_1 in upland cotton (Gossypium hirsutum)

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Chinese Science Bulletin 2009年第9期54卷 1529-1533页

作者： ZHAO Liang CAI CaiPing ZHANG TianZhen guo wangzhen National Key Laboratory of Crop Genetics & Germplasm Enhancement Cotton Research Institute Nanjing Agricultural University Nanjing 210095 China

Sub 16 is a substitution line with G. hirsutum cv. TM-1 genetic background except that the 16th chro-mosome (Chr. 16) is replaced by the corresponding homozygous chromosome of G. barbadense cv. 3-79, and T586 is a G. hirsutum multiple gene marker line with 8 dominant mutation genes. The R1 gene for anthocyanin pigmentation was tagged in Chr. 16 in T586. The objective of this research was to screen SSR markers tightly linked with R1 by using the F2 segregating population containing 1259 plants derived from the cross of Sub 16 and T586 and the backbone genetic linkage map from G. hir-sutum×G. barbadense BC1 newly updated by our laboratory. Genetic analysis suggested that the seg-regation ratio of red plants in the F2 population fit Mendelian 1:2:1 inheritance, confirming that the red plant trait was controlled by an incomplete dominance gene. Preliminary mapping of R1 was conducted using 237 randomLy selected F2 individuals and JoinMap v3.0 software. Then, a fine map of R1 was constructed using the F2 segregating population containing 1259 plants, and R1 was located between NAU4956 and NAU6752, with only 0.49 cM to the nearest maker loci (NAU6752). These results pro-vided a foundation for map-based cloning of R1 and further development of cotton cultivars with red fibers by transgenic technology.

关键词：植物基因棉花品种陆地棉精细定位遗传连锁图谱基因标记 SSR标记转基因技术

Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton

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Chinese Science Bulletin 1998年第1期43卷 52-54页

作者： wangzhen guo Tianzhen Zhang Jiaju Pan Russell J. Kohel Nanjing Agr Univ Cotton Genet & Breeding Res Lab Nanjing 210095 Peoples R China USDA ARS So Crops Res Lab College Stn TX 77845 USA

Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC\-3 segregating population of （06132R×Simian No. 3）. Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of （Zhong 12A1×06132R）F\-2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV15 300 was linked with the fertilityrestoring gene （R f \-1） with a recombination value of 13.0±2.57%.

关键词： Gossypium hirsutum \% upland cotton fertility_restoring gene RAPD marker.

Variation induced by DNA rearrangement in a transgenic Bt+CpTI cotton strain

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Chinese Science Bulletin 2001年第7期46卷 559-562页

作者： wangzhen guo Tianzhen Zhang Xiefei Zhu Xiaoling Yuan Key Laboratory of Crop Germplasm and Breeding Ministry of Agriculture Department of Agronomy Nanjing Agriculture University Nanjing China

In the development, of transgenic Bt + CpTI cotton cultivars, one male and female sterile mutant has been found in a homozygous T4 strain in our laboratory. The mutant plant, as well as its leaves, buds and flowers, is only 1/2-1/3 as large as that of the wild transgenic Bt + CpTI bivalant cotton plants. Cytological observation found that the chromosome number of the mutant is 2n=52; however, there are 4-8 univalents observed in meiosis I of pollen mother cells. Laboratory bioassay indicated that the mutant was highly resistant to bollworm as the wild plants. PCRamplification revealed that Bt and CpTl genes in the mutant were still intactly inserted. However, small deletion of flanked area had been observed in the mutant by Southern blotting analysis. So it is proposed that the mutant pheno-type might result from either the DNA deletion or T-DNA transferring in plant genome. No such report has been presented that the rearrangement of chromosome structure in a homozygous transgenic line occurred.

关键词： transgenic insect-resistant cotton mutant variation DNA rearrangement.

Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton

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The Crop Journal 2024年第2期12卷 411-421页

作者： Yujia Duan Xiaoguang Shang Ruiping Tian Weixi Li Xiaohui Song Dayong Zhang wangzhen guo State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Nanjing Agricultural UniversityNanjing 210095JiangsuChina Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application Nanjing Agricultural UniversityNanjing 210095JiangsuChina

Mitochondrial calcium uniporter(MCU)is a conserved calcium ion(Ca^(2+))transporter in the mitochondrial inner membrane of eukaryotic *** MCU proteins regulate Ca^(2+)flow and modulate plant cell development remain largely ***,we identified the gene GhMCU4 encoding a MCU protein that negatively regulates plant development and fiber elongation in cotton(Gossypium hirsutum).GhMCU4expressed constitutively in various tissues with the higher transcripts in elongating fiber *** of GhMCU4 in cotton significantly elevated the plant height and root *** calcium signaling pathway was significantly activated and calcium sensor genes,including Ca^(2+)dependent modulator of interactor of constitutively active ROP(GhCMI1),calmodulin like protein(GhCML46),calciumdependent protein kinases(GhCPKs),calcineurin B-like protein(GhCBLs),and CBL-interacting protein kinases(GhCIPKs),were dramatically upregulated in GhMCU4-silenced *** processes were preferentially enriched,and genes related to regulation of transcription were upregulated in GhMCU4-silenced *** contents of Ca^(2+)and H_(2)O_(2)were significantly increased in roots and leaves of GhMCU4-silenced *** length and Ca^(2+)and H_(2)O_(2)contents in fibers were significantly increased in GhMCU4-silenced *** study indicated that GhMCU4 plays a negative role in regulating cell elongation in cotton,thus expanding understanding in the role of MCU proteins in plant growth and development.

关键词： Calcium signaling Hydrogen peroxide Metabolic processed Gossypium hirsutum

The R2R3-MYB transcription factor GaPC controls petal coloration in cotton

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The Crop Journal 2023年第5期11卷 1319-1330页

作者： Caiping Cai Fan Zhou Weixi Li Yujia Yu Zhihan Guan Baohong Zhang wangzhen guo State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Nanjing Agricultural UniversityNanjing 210095JiangsuChina Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application Nanjing Agricultural UniversityNanjing 210095JiangsuChina Department of Biology East Carolina UniversityGreenvilleNC 27858USA

Although a few cases of genetic epistasis in plants have been reported, the combined analysis of genetically phenotypic segregation and the related molecular mechanism remains rarely studied. Here, we have identified a gene(named GaPC) controlling petal coloration in Gossypium arboreum and following a heritable recessive epistatic genetic model. Petal coloration is controlled by a single dominant gene,GaPC. A loss-of-function mutation of GaPC leads to a recessive gene Gapc that masks the phenotype of other color genes and shows recessive epistatic interactions. Map-based cloning showed that GaPC encodes an R2R3-MYB transcription factor. A 4814-bp long terminal repeat retrotransposon insertion at the second exon led to GaPC loss of function and disabled petal coloration. GaPC controlled petal coloration by regulating the anthocyanin and flavone biosynthesis pathways. Expression of core genes in the phenylpropanoid and anthocyanin pathways was higher in colored than in white petals. Petal color was conferred by flavonoids and anthocyanins, with red and yellow petals rich in anthocyanin and flavonol glycosides, respectively. This study provides new insight on molecular mechanism of recessive epistasis,also has potential breeding value by engineering GaPC to develop colored petals or fibers for multifunctional utilization of cotton.

关键词： Cotton Petal color R2R3-MYB transcription factor LTR-RT insertion Flavonoid/anthocyanin biosynthesis Recessive epistasis

Mining elite loci and candidate genes for root morphology-related traits at the seedling stage by genome-wide association studies in upland cotton(Gossypium hirsutum L.)

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Journal of Integrative Agriculture 2024年第10期23卷 3406-3418页

作者： Huaxiang Wu Xiaohui Song Muhammad Waqas-Amjid Chuan Chen Dayong Zhang wangzhen guo State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization/Engineering Research Center of Cotton Germplasm Enhancement and Application Ministry of Education/Nanjing Agricultural UniversityNanjing 210095China

Root system architecture plays an essential role in water and nutrient acquisition in plants,and it is significantly involved in plant adaptations to various environmental *** this study,a panel of 242 cotton accessions was collected to investigate six root morphological traits at the seedling stage,including main root length(MRL),root fresh weight(RFW),total root length(TRL),root surface area(RSA),root volume(RV),and root average diameter(AvgD).The correlation analysis of the six root morphological traits revealed strong positive correlations of TRL with RSA,as well as RV with RSA and AvgD,whereas a significant negative correlation was found between TRL and ***,a genome-wide association study(GWAS)was performed using the root phenotypic and genotypic data reported previously for the 242 accessions using 56,010 single nucleotide polymorphisms(SNPs)from the CottonSNP80K array.A total of 41 quantitative trait loci(QTLs)were identified,including nine for MRL,six for RFW,nine for TRL,12 for RSA,12 for RV and two for *** them,eight QTLs were repeatedly detected in two or more *** these results with a transcriptome analysis,we identified 17 candidate genes with high transcript values of transcripts per million(TPM)≥30 in the ***,we functionally verified the candidate gene GH_D05G2106,which encodes a WPP domain protein 2in root development.A virus-induced gene silencing(VIGS)assay showed that knocking down GH_D05G2106significantly inhibited root development in cotton,indicating its positive role in root system architecture ***,these results provide a theoretical basis and candidate genes for future studies on cotton root developmental biology and root-related cotton breeding.

关键词： cotton root-morphology traits quantitative trait loci candidate genes GWAS

Genome-wide analysis of the WRKY transcription factor gene family in Gossypium raimondii and the expression of orthologs in cultivated tetraploid cotton

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The Crop Journal 2014年第Z1期2卷 87-101页

作者： Caiping Cai Erli Niu Hao Du Liang Zhao Yue Feng wangzhen guo State Key Laboratory of Crop Genetics & Germplasm Enhancement Hybrid Cotton R&D Engineering Research CenterMOENanjing Agricultural University

WRKY proteins are members of a family of transcription factors in higher plants that function in plant responses to various physiological *** identified 120 candidate WRKY genes from Gossypium raimondii with corresponding expressed sequence tags in at least one of four cotton species,Gossypium hirsutum,Gossypium barbadense,Gossypium arboreum,and *** WRKY members were anchored on 13 chromosomes in *** with uneven *** analysis showed that WRKY candidate genes can be classified into three groups,with 20 members in group I,88 in group II,and 12 in group ***88 genes in group II were further classified into five subgroups,groups IIa–e,containing 7,16,37,15,and 13 members,*** characterized diversity in amino acid residues in the WRKY domain and/or other zinc finger motif regions in the WRKY *** expression patterns of WRKY genes revealed their important roles in diverse functions in cotton developmental stages of vegetative and reproductive growth and stress *** and expression analyses show that WRKY proteins are a class of important regulators of growth and development and play key roles in response to stresses in cotton.

关键词： Expression pattern Gossypium Phylogenetic relationship Stress WRKY transcription factors

Gene cloning and molecular breeding to improve fiber qualities in cotton

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Chinese Science Bulletin 2003年第8期48卷 709-717页

作者： guo wangzhen, SUN Jing & ZHANG Tianzhen National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China Correspondence should be addressed to guo wangzhen (e-mail: cotton @***) National Key Laboratory of Crop Genetics & Germplasm Enhancement Cotton Research Institute Nanjing Agricultural University China

Cotton fiber is one of known natural resources comprising the highest purity cellulose. It plays an important role worldwide in the textile industry. With the acceleration of spinning speeds and the improvement of the peoples liv-ing level, the demand of improving cotton fiber qualities is getting stronger and stronger. So, making clear the develop-mental model of fiber cell and elucidating systematically the molecular mechanisms of cotton fiber development and regulation will produce a great significance to make full use of cotton gene resources, raise cotton yield and improve fiber quality, and even develop man-made fiber. In the paper, the status of the gene cloning and the molecular breeding to im-prove cotton fiber quality were reviewed, the importance and potential of gene cloning related with cotton fiber quality were put forward and the proposal and prospect on fiber quality improvement were made. Using national resources available and through the creative exploration in corre-sponding research, some international leading patents in genes or markers linked with cotton fiber development hav-ing Chinese own intellectual property should be licensed quickly. And they can be used to improve cotton fiber quality in cotton breeding practice.

关键词：基因克隆技术棉花纤维质量分子育种技术

Inheritance of resistance to Helicoverpa armigera of 3 kinds of transgenic Bt strains available in upland cotton in China

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Chinese Science Bulletin 2000年第4期45卷 363-367页

作者： TANG Canming SUN Jing ZHU Xiefei guo wangzhen ZHANG Tianzhen SHEN Jinliang GAO Congfen ZHOU Weijun CHEN Zhixian guo Sandui Key Laboratory of Crop Germplasm and Breeding Ministry of Agriculture Department of Agronomy Nanjing Agricultural UniversityNanjing 210095China Department of Plant Protection Nanjing Agricultural UniversityNanjing 210095China Cotton Research Institute Shanxi Academy of Agricultural SciencesYuncheng 044000China Biotechnology Research Centre Chinese Academy of Agricultural SciencesBeijing 100081China

There are 3 kinds of transgenic Bt strains, Shanxi 94-24, Zhongxin 94, and R19, in upland cotton in China. Their transgenic Bt insect-resistance cultivars or hybrids have been developed and grown by farmers. Genetic studies indicate that the resistance of the 3 transgenic Bt cotton strains to Helicoverpa armigera is controlled by one pair of non-allelic dominant genes. Linkage relationship between the resistant genes of R19 and Shanxi 94-24 transgenic Bt strains shows that they may be inserted in the same chromosome. FT hybrids crossed among the 3 strains show that high levels of protection from feeding damage are the same as that of their parents. Therefore, there is no co-suppression phenomenon in many transgenic plants. The results presented here afford a fundamental reliance in developing transgenic Bt insect-resistant cultivars and exploiting the heterosis of hybrids in upland cotton.

关键词： upland cotton transgenic Bt plant inheritance insect-resistance Helicoverpa armigera.