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Molecular beacon-assisted isothermal amplification combined with nucleic acid lateral flow technology for the detection of multiple bacterial contaminations in platelets

Molecular beacon-assisted isothermal amplification combined ...

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2012全国临床微生物与感染免疫学术研讨会

作者： wang xiaohui,wang jidong,li yuan,zhan linsheng beijing institute of transfusion medicine

Bacterial infections still pose a major risk for the safety of the blood supply,especially for the platelet concentrates(PCs),as they are stored at room temperature for 5-7 days.A lot of works based on culture method have been done to test the sterility of the PCs,but most of them have to take at least 24h which is too long when compare to the short shift time of the *** species of bacteria could contaminate the PCs,it is really hard to identify a kind of antigen or protein that conserved in all species of bacteria serologically if it is not impossible. So,more and more researchers tend to use molecular diagnostic technologies to solve these problems. A trend evident in disease diagnosis field in the past two decades is that the molecular diagnostic technologies such as polymerase chain reaction(PCR) are edging out the serological method,because they are rapid,specific and most importantly *** the requirement of the expensive equipment and skillful workers restricted this method to only be applied in medical center or research ***,several isothermal amplification methods had been invented since *** typical ones include Strand Displacement Amplification(SDA), Nucleic Acid Sequence Based Amplification(NASBA) and Loop-Mediated Isothermal Amplification(LAMP) etc. Without of the need of precision instrument and skillful worker,these methods could detect as low as one copy of target genes at isothermal condition in about 2 *** our knowledge,the 16S rDNA sequence exists usually in most types of bacteria and often provides general information for organism *** 16S rDNA-based realtime PCR method is also considered as a useful tool for rapid screening of bacterial contaminations in blood ***,we employed the conserved 24-base fragment in it as a detection *** the mentioned isothermal amplification methods are sensitive and easy to use,they also have some disadvantages of which the primer design is the mo

关键词： Beacon A mplification Bacterial contamination Detection Platelets

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Research on the Effect of Stored Red Blood Cell on the Migration and Homing of Immature Dendritic Cell

Research on the Effect of Stored Red Blood Cell on the Migra...

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第十一届全国免疫学学术大会

作者： zhan linsheng wang xiaohui Zhao Man beijing transfusion institute

Objective:To investigate whether the migration model/patterns of immature dendritic cell vaccines(im DC)could be affected by stored red blood cell(RBC)in ***:We cultured im DC with fresh RBC,old RBC and PBS for 10h,and then detected IL-12p70,IL-1β,TNF-α,IL-6,IL-10 with commercially available EliSA kits,and analyzed the allostimulatory molecules and chemokine

关键词： Dendritic cells stored red blood cell migration Bioluminescence Imaging

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Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced antiviral therapeutic effects

Mature dendritic cell derived from cryopreserved immature de...

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第十一届全国免疫学学术大会

作者： zhan linsheng Zhou Qianqian zhang Yulong wang xiaohui beijing transfusion institute

Objective: Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell(DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy;however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DC matured from cryopreserved immature DCs(cryoim-m DCs) remain to be ***: In the present study, we compared cryoim-m DCs with DCs matured from fresh immature DCs(fm DCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. Results: The results show that cryopreservation impair the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8 T cell response induced by cryoim-m DCs was much weaker than that induced by fm DCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. Conclusions: In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DC matured from frozen immature DCs were hindered to some extent.

关键词： DCs cryopreservation migration bioluminescence imaging anti-viral effects

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全血在应急输血救治中的应用进展

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中国输血杂志 2019年第6期32卷 598-602页

作者：王政军张玉龙周欠欠王小慧詹林盛军事科学院军事医学研究院卫生勤务与血液研究所

输血救治是抢救重度出血伤员的重要环节。随着输血医学的发展和进步,人类输血历史从最初的全血输注逐渐发展为成分血输注。而近年来美、英、法等国家在战场上院前救治中使用全血得到更好损伤控制复苏效果的经验使全血重新受到了输血医... 详细信息

输血救治是抢救重度出血伤员的重要环节。随着输血医学的发展和进步,人类输血历史从最初的全血输注逐渐发展为成分血输注。而近年来美、英、法等国家在战场上院前救治中使用全血得到更好损伤控制复苏效果的经验使全血重新受到了输血医学界重视。本文主要通过全血输注优势、全血的采集与存储、全血的战时应用与临床疗效以及全血相关实验研究4个方面来介绍紧急状态下全血输注带给重度出血伤员的益处,它可以改善伤员的预后,提高伤员的生存率。

关键词：全血院前输血应急输血

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