Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced antiviral therapeutic effects
作者单位：Beijing transfusion institute
会议名称：《第十一届全国免疫学学术大会》
会议日期：2016年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：DCs cryopreservation migration bioluminescence imaging anti-viral effects
摘 要：Objective: Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell(DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy;however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DC matured from cryopreserved immature DCs(cryoim-m DCs) remain to be ***: In the present study, we compared cryoim-m DCs with DCs matured from fresh immature DCs(fm DCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. Results: The results show that cryopreservation impair the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8 T cell response induced by cryoim-m DCs was much weaker than that induced by fm DCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. Conclusions: In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DC matured from frozen immature DCs were hindered to some extent.