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Detection of seven phytohormones in peanut tissues by ultra-highperformance liquid chromatography-triple quadrupole tandem mass spectrometry

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Journal of Integrative Agriculture 2020年第3期19卷 700-708页

作者： wang hai-xia wang Ming-lun wang Xiu-zhong DING Yu-long College of Biology and the Environment Nanjing Forestry UniversityNanjing 210037P.R.China College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdao 266109P.R.China Co-innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037P.R.China

Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural *** this study,an ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry method was established for separation and quantification of trans-zeatin,trans-zeatin riboside,gibberellin A3,indol-3-acetic acid,salicylic acid,abscisic acid,and jasmonic acid(JA) without any *** sepa ration was performed on an Agilent Explus Plus C18 column by using methanol and water as mobile phases with gradient *** target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin,transzeatin riboside,indole-3-acetic acid,and via negative electrospray ionization for gibberellin3,salicylic acid,abscisic acid,and *** limits of detection ranged from 0.0127 ng L^-1 for gibberellin A3(GA3) to 33.26 ng L^-1 for JA and were lower than the currently reported values in *** proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and *** recoveries of the spiked phytohormones ranged from 80.20 to102.56%.The contents of seven endogenous hormones varied specifically in different development stages of *** study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit,which are controlled by seven endogenous hormones.

关键词： phytohormones simultaneous detection UHPLC-MS/MS peanut

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新质生产力对马克思生产力理论的守正创新

哈尔滨市委党校学报 2025年第01期 21-26页

作者：王海霞林颖谈小钰兰州理工大学马克思主义学院

新质生产力是在马克思生产力理论的基础上，结合现代社会和科技发展的实际情况，对生产力概念进行的深化和拓展，强调了人类自主活动的能力和其产生的实际成果，以及各种生产要素在生产实践中的相互作用。在新时代条件下，不断推动战略... 详细信息

新质生产力是在马克思生产力理论的基础上，结合现代社会和科技发展的实际情况，对生产力概念进行的深化和拓展，强调了人类自主活动的能力和其产生的实际成果，以及各种生产要素在生产实践中的相互作用。在新时代条件下，不断推动战略性新兴产业的培养与发展，是国家经济发展中的重要战略举措，与马克思生产力理论的内在逻辑关系紧密相连，体现了生产力发展的必然趋势与规律。新质生产力不仅是对马克思生产力理论的守正创新，更是指导中国在新时代背景下实现经济高质量发展的重要理论工具。

关键词：新质生产力马克思生产力理论守正创新

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Synthesis and anti-HBV activity of S-substituted 7-mercapto-4-methylcoumarin analogs

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Chinese Chemical Letters 2008年第8期19卷 925-927页

作者： Ying Chen Jin Zhou hai wang Yi xia Zheng Yu Yang Peng xia Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China

Twelve S-substituted 7-mercapto-4-methylcoumarin analogs were synthesized and evaluated for the inhibition to HBV in HepG2 2.***.1.5 cell. Among them, ten compounds exhibited potent inhibition to HBsAg and/or HBeAg with the IC50 values of sub μmol/L level. The ICso of anti-HBsAg activities of 5c and 5j reached 0.01 μmol/L respectively which were 16 fold more potent than that of 3TC. Compounds 3, 5e, 5g, 5h and 5i showed admirable inhibitory activity to both HBsAg and HBeAg. The bioassay results indicated the S-substituted 7-mercapto-4-methylcoumarin analogs merit attention as novel anti-HBV agents.

关键词： Synthesis S-Substituted 7-mercapto-4-methylcoumarin Anti-HBV

Evaluating the Nutritional Status of Oncology Patients and Its Association with Quality of Life

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Biomedical and Environmental Sciences 2018年第9期31卷 637-644页

作者： ZHANG Ya Hui XIE Fang Yi CHEN Ya Wen wang hai xia TIAN Wen xia SUN Wen Guang WU Jing Department of Clinical Nutrition International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University Department of Clinical Nutrition Shanghai Jiao Tong University Affiliated with Sixth People's Hospital Department of Clinical Nutrition Guangdong Medical University Affiliated Zhongshan Chenxinghai Hospital

Objective The primary aim of the study was to compare two nutritional status evaluation tools： the Patient-Generated Subjective Global Assessment（PG-SGA） and Nutritional Risk Screening（NRS-2002）. Using the European Organization for Research and Treatment of Cancer Quality of Life Core Questionnaire 30（EORTC QLQ-C30）, the second aim was to provide constructive advice regarding the quality of life of patients with malignancy. Methods This study enrolled 312 oncology patients and assessed their nutritional status and quality of life using the PG-SGA, NRS-2002, and EORTC QLQ-C30. Results The data indicate that 6% of the cancer patients were well nourished. The SGA-A had a higher sensitivity（93.73%） but a poorer specificity（2.30%） than the NRS-2002（69.30% and 25.00%, respectively） after comparison with albumin. There was a low negative correlation and a high similarity between the PG-SGA and NRS-2002 for evaluating nutritional status, and there was a significant difference in the median PG-SGA scores for each of the SGA classifications（P 〈 0.001）. The SGA-C group showed the highest PG-SGA scores and lowest body mass index. The majority of the target population received 2 points for each item in our 11-item questionnaire from the EORTC QLQ-C30. Conclusion The data indicate that the PG-SGA is more useful and suitable for evaluating nutritional status than the NRS-2002. Additionally, early nutrition monitoring can prevent malnutrition and improve the quality of life of cancer patients.

关键词： Malnutrition PG-SGA NRS-2002 EORTC QLQ-C30 Malignant patients Nutritional assessment

Effects of chebulinic acid on differentiation of human leukemia K562 cells

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Acta Pharmacologica Sinica 2004年第2期25卷 105-112页

作者： Zong-chun YI, Zhao wang2, hai-xia LI, Ming-jie LIU, Rong-cong WU, xiao-hui wang Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

AIM: To study effects of chebulinic acid on erythroid and megakaryocytic differentiation in K562 cells. METHODS: The benzidine staining method was used to evaluate hemoglobin synthesis; the expression of erythroid specific glycophorin A (GPA) protein and megakaryocytic surface marker CD61 was determined by flow cytometry using fluorescence labeled antibodies; erythroid and megakaryocytic mRNA expression was analyzed by RT-PCR. RESULTS: During erythroid differentiation induced by butyric acid (BA) or hemin, chebulinic acid not only inhib- ited the hemoglobin synthesis of BA- and hemin-treated K562 cells in concentration-dependant manner with IC50 of 4 μmol/L and 40 μmol/L respectively, but also inhibited another erythroid differentiation marker acetylcholinesterase at the concentration of 50 μmol/L in the cells either treated or untreated with each erythroid differentiation inducers, whereas chebulinic acid 50 μmol/L did not change GPA protein expression in these cells significantly. When K562 cells were treated with TPA 50 μg/L for 72 h to induce megakaryocytic differentiation, the presence of chebulinic acid 50 μmol/L slightly provoked the decrease of GPA protein expression induced by TPA. Chebulinic acid did not change the TPA-induced CD61 expression at the same concentration. Chebulinic acid also reduced the mRNA levels of erythroid relative genes including γ-globin, PBGD, NF-E2, and GATA-1 genes in K562 cells either treated or untreated with BA, whereas chebulinic acid upregulated the mRNA levels of GATA-2 transcription factor in these cells. CONCLUSION: Chebulinic acid had inhibitory effect on erythroid differentiation likely through changing transcriptional activation of differentiation relative genes, which suggests that chebulinic acid or other tannins might influence the efficiency of some anti-tumor drugs-induced differentiation or the hematopoiesis processes.

关键词： K562cells chebulinic acid butyrates hemin glycophorin CD61 antigen gene expression regulation celldifferentiation

The High Nitrogen Pressure Synthesis of Manganese Nitride

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Chinese Physics Letters 2012年第12期29卷 228-231页

作者： SI Ping-Zhan JIANG Wei wang hai-xia ZHONG Min GE Hong-Liang CHOI Chul-Jin LEE Jung-Goo School of Materials Science and Engineering China Jiliang UniversityHangzhou 3100182Korea Institute of Materials ScienceChangwonGyeongnam641-831R.Korea

We report on the structural transformation and unique magnetic properties of manganese nitrides prepared by nitriding Mn under N_(2) pressures of up to 25 MPa with varying *** N_(2) pressure not only makes nitridation more efficient at lower temperatures,but also enhances the N-content in the nitride lattices,which were expanded with increasing *** N-rich nitrides,includingε-Mn_(4)N,ζ-Mn6N_(2).58 andη-Mn_(3)N_(2),exhibit unique thermal *** N-richε-phase exhibits much larger coercivity and lower saturation magnetization in comparison with theε-phase prepared under ambient N_(2) *** coercivity of the N-richζ-phase reaches up to 45054 A/m.A saturation magnetization as large as 31 Am^(2)/kg is observed in the N-richη-*** are quite different from the conventional antiferromagneticζ-andη-phase obtained under ambient N_(2) *** ascribe the unconventional magnetic properties of the nitrides to the lattice distortion originating from the N-enrichment.

关键词： transformation magnetization saturation

The Bidirectional Effects of Arsenic on miRNA-21: A Systematic Review and Meta-analysis

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Biomedical and Environmental Sciences 2018年第9期31卷 654-666页

作者： LIU Jia Qing NIU Qing HU Yun Hua LI Yu wang hai xia XU Shang Zhi DING Yu Song LI Shu Gang MA Ru Lin Department of Public Health School of Medicine Shihezi University

Objective Arsenic is a metalloid environmental carcinogen involved in the occurrence and development of many cancers. miRNA-21 plays a crucial role in arsenic-induced carcinogenesis. We aimed to elucidate the mechanism by which miRNA-21 influences arsenic-induced cancer. Methods We used meta-analysis of published studies to determine how arsenic induces cancerous cells through miRNA-21. Results Low-dose arsenic exposure（≤ 5 μmol/L） can increase miRNA-21 and phosphorylated signal transducter and activator of transcription 3（pSTAT3） expression, and decrease programmed cell death protein 4（PDCD4） and protein sprouty homolog 1（Spry1） expression. High-dose arsenic exposure（〉 5 μmol/L）, can increase miRNA-21 expression, and decrease Spry1 and E-cadherin expression. Short-term arsenic exposure（≤ 24 h） can increase miRNA-21 and pS TAT3 expression, and decrease PDCD4 expression. Moreover, long-term arsenic exposure（〉 24 h） can increase the miRNA-21, STAT3, and pSTAT3 expression, and decrease PDCD4 expression. We found that activation of miRNA-21 and pSTAT3 were most pronounced following long-term arsenic exposure at low doses, and the effects on PDCD4 expression were most pronounced following short-term arsenic exposure at low doses. miRNA-21 inhibitors increased the expression of tumor suppressor genes PDCD4, PTEN, and Spry1 and miRNA-21-mimics suppressed the expression of these tumor suppressor genes. Conclusion Arsenic can cause cancer by activating miRNA-21 and inhibiting the expression of PDCD4, PTEN, and Spry1.

关键词： Arsenic miRNA-21 Meta-analysis PDCD4 PTEN Spry1

Research of Seed Testa Structure and Storage Material of Peanut Germplasm with Different Resistance to A. flavus

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Agricultural Sciences in China 2006年第6期5卷 478-482页

作者： SHAN Shi-hua wang hai-xia LI Chun-juan WAN Shu-bo LIU Hong-tao JIANG Guo-yong Shandong Peanut Research Institute Qingdao 266100 P.R.China Life Institute Laiyang Agricultural College Laiyang 265200 P.R. China

There was an obvious relationship between seed testa structure, storage material and resistance to A. flavus of peanut. Results showed that seed testa of peanut germplasm with high resistance （HR） to A. flavus infection had thicker wax layer, integrated and tight epidermis layer, regular vascular tissue range. However, the seed testa of peanut germplasm with high sensitivity （HS） to A. flavus had the reverse results, and results of those with medium resistance （MR） to A. flavus lay in between, but changes of testa thickness were not significant among different resistance kinds. Results also showed that some seed storage materials were closely related with resistance potential to A. flavus. It seemed that varieties with higher resistance to A. flavus had higher oleic acid and protein content, lower linoleic acid and fat content. Content of palm acid, total sugar and VE did not show positive relationship with the resistance to A. flavus.

关键词： peanut germplasm resistance to A. flavus seed testa structure and storage material

Grape Seed Procyanidin Extract Reduces Arsenic- Induced Renal Inflammatory Injury in Male Mice

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Biomedical and Environmental Sciences 2017年第7期30卷 535-539页

作者： wang Cheng LI Jun SONG Guan Ling NIU Qiang XU Shang Zhi FENG Gang Ling wang hai xia LI Yu LI Shu Gang LI Feng Department of Public Health and Key Laboratory of Xinjiang Endemic and Ethnic Diseases (Ministry of Education) Shihezi University School of Medicine Shihezi 832002 Xinjiang China Department of Medical Imageing of the First Affiliated Hospital Shihezi University School of Medicine Shihezi 832002 Xinjiang China Department of Pathology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases {Ministry of Education) Shihezi University School of Medicine Shihezi 832002 Xinjiang China Department of Pathology of Beijing chaoyang hospital the Capital Medical University Beijing 100020 China

The aim of the present study is to evaluate the ability and mechanism by which grape seed procyanidin extract （GSPE） relieves arsenic trioxide （As2O3）-induced renal inflammatory injury. Therefore, male Kunming mice were treated with As2O3 and/or GSPE by gavage for 5 weeks. Mice were then sacrificed and inflammatory cytokines of kidneys were examined by ELISA, whereas the expression levels of molecules involved in the nuclear factor （NF）-KB signaling pathway were evaluated by both qRT-PCR and Western blot. Our results indicate that GSPE prevents As2O3-mediated renal inflammatory injury by inhibiting activation of the NF-KB signaling pathway and inflammatory cytokine production, while promoting expression of anti-inflammatory cytokines.

关键词： in were Grape Seed Procyanidin Extract Reduces Arsenic-Induced Renal Inflammatory Injury in Male Mice GSPE IKK NF of Figure As