Detection of seven phytohormones in peanut tissues by ultra-highperformance liquid chromatography-triple quadrupole tandem mass spectrometry

作     者：WANG Hai-xia WANG Ming-lun WANG Xiu-zhong DING Yu-long WANG Hai-xia;WANG Ming-lun;WANG Xiu-zhong;DING Yu-long

作者机构：College of Biology and the EnvironmentNanjing Forestry UniversityNanjing 210037P.R.China College of Chemistry and Pharmaceutical SciencesQingdao Agricultural UniversityQingdao 266109P.R.China Co-innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing 210037P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2020年第19卷第3期

页      面：700-708页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the earmarked fund for China Agriculture Research System (CARS-14) partly supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (RAPD) the Natural Science Foundation of Shandong Province, China (ZR2018MB030) 

主　　题：phytohormones simultaneous detection UHPLC-MS/MS peanut 

摘      要：Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural *** this study,an ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry method was established for separation and quantification of trans-zeatin,trans-zeatin riboside,gibberellin A3,indol-3-acetic acid,salicylic acid,abscisic acid,and jasmonic acid(JA) without any *** sepa ration was performed on an Agilent Explus Plus C18 column by using methanol and water as mobile phases with gradient *** target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin,transzeatin riboside,indole-3-acetic acid,and via negative electrospray ionization for gibberellin3,salicylic acid,abscisic acid,and *** limits of detection ranged from 0.0127 ng L^-1 for gibberellin A3(GA3) to 33.26 ng L^-1 for JA and were lower than the currently reported values in *** proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and *** recoveries of the spiked phytohormones ranged from 80.20 to102.56%.The contents of seven endogenous hormones varied specifically in different development stages of *** study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit,which are controlled by seven endogenous hormones.