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A chorismate mutase from Radopholus similis plays an essential role in pathogenicity

Journal of Integrative Agriculture 2024年第3期23卷 923-937页

作者： Sihua Yang Junyi Li Shuai Yang Shiqiao Tang huizhong wang Chunling Xu Hui Xie Laboratory of Plant Nematology andResearch Centerof Nematodes of Plant Quarantine Department of Plant Patholo of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China

In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study,based on transcriptome data, a chorismate mutase gene of Radopholus similis(RsCM) was identified and cloned,which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity(PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference(RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid(SA) pathway genes(PR1 and PR5) in roots infected by the nematode were significantly down-regulated,which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.

关键词： Radopholus similis chorismate mutase plant defense transgenic tomato

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基于改进YOLOv4算法的遥感图像飞机目标检测

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计算机与数字工程 2024年第2期52卷 416-422页

作者：王惠中文学兰州理工大学电气工程与信息工程学院兰州730050 兰州理工大学甘肃省工业过程先进控制重点实验室兰州730050

针对在遥感图像上对飞机目标检测的精度低问题,论文通过对PANet特征融合网络结构的加深使得YOLOv4算法对小目标的检测更加敏感,进而提高算法的平均检测精度;另外,利用K-means++算法产生了能够自适应与数据集的检测先验框以减少YOLOv4检... 详细信息

针对在遥感图像上对飞机目标检测的精度低问题,论文通过对PANet特征融合网络结构的加深使得YOLOv4算法对小目标的检测更加敏感,进而提高算法的平均检测精度;另外,利用K-means++算法产生了能够自适应与数据集的检测先验框以减少YOLOv4检测算法对边界框回归损失计算过程中的冗余。在RSOD(Remote Sensing Object Detection)数据集上的对比实验表明,综合改进后的YOLOv4算法AP值达到了80.25%。特别地,改进后的YOLOv4算法对小目标检测的置信度得分较高。

关键词：遥感图像目标检测 YOLOv4 特征融合

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基于深度学习的电机故障诊断

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计算机与数字工程 2024年第5期52卷 1536-1540页

作者：王晓兰马泽娟王惠中兰州理工大学兰州730050

故障诊断在保证电机的稳定运行中占据着非常重要的地位,因此,故障诊断在当前的研究中是一个热点。该研究利用短时傅里叶变换把一维的振动信号转换成二维的时频图,进而解决电机轴承的振动信号的非线性和不稳定性问题,并且作为卷积神经网... 详细信息

故障诊断在保证电机的稳定运行中占据着非常重要的地位,因此,故障诊断在当前的研究中是一个热点。该研究利用短时傅里叶变换把一维的振动信号转换成二维的时频图,进而解决电机轴承的振动信号的非线性和不稳定性问题,并且作为卷积神经网络的输入,通过对故障特征信号的直接提取,来形成样本数据集,通过卷积神经网络与softmax多分类器来建立故障诊断模型,在Python中验证该算法优化的准确性,证明了该算法可以提高电机故障诊断的准确率。

关键词：卷积神经网络 softmax多分类器故障诊断短时傅里叶变换

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Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation

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Frontiers of Environmental Science & Engineering 2016年第2期10卷 368-380页

作者： Min GOU Jing ZENG huizhong wang Yueqin TANG Toru SHIGEMATSU Shigeru MORIMURA Kenji KIDA College of Architecture and Environment Sichuan University Chengdu 610065 China Department of Food Science Faculty of Applied Life Sciences Niigata University of Pharmacy and Applied Life Sciences (NUPALS) Niigata 956-8603 Japan Graduate School of Science and Technology Kumamoto University Kumamoto 860-0862 Japan

The microbial community structures of two mesophilic anaerobic chemostats, one fed with glucose, the other with starch as sole carbon sources, were studied at various dilution rates （0.05-0.25 d-1 for glucose and 0.025-0.1 d-1 for starch） during two years continuous operation. In the glucose-fed chemostat, the aceticlastic methanogen Methanosaeta spp. and hydrogenotrophic methanogen Methanoculleus spp. predominated at low dilution rates, whereas Methanosaeta spp. and the hydrogenotrophic Methanobacterium spp. predominated together when dilution rates were greater than 0.1 d 1. Bacteria affiliated with the phyla Bacteroidetes, Spiro- chaetes, and Actinobacteria predominated at dilution rates of 0.05, 0.1, and 0.15 d-l, respectively, while Firmicutes predominated at higher dilution rates （0.2 and 0.25 d-l）. In the starch-fed chemostat, the aceticlastic and hydrogeno- trophic methanogens coexisted at all dilution rates. Although bacteria belonging to only two phyla were mainly responsible for starch degradation （Spirochaetes at the dilution rate of 0.08 d-1 and Firmicutes at other dilution rates）, different bacterial genera were identified at different dilution rates. With the exception of Archaea in the glucose-fed chemostat, the band patterns revealed by denaturing gradient gel electrophoresis （DGGE） of the microbial communities in the two chemostats displayed marked changes during long-term operation at a constant dilution rate. The bacterial community changed with changes in the dilution rate, and was erratic during long- term operation in both glucose-fed and starch-fed chemo- stats.

关键词： microbial community glucose degradation,starch degradation dilution rate continuous methanefermentation phylogenetic analysis

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Salt tolerance of transgenic rice (Oryza sativa L.) with mtlD gene and gutD gene

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Chinese Science Bulletin 2000年第18期45卷 1685-1690页

作者： wang huizhong HUANG Danian LU Ruifang LIU Junjun QIAN Qian PENG Xuexian Department of Biology Hangzhou Teacher’s CollegeHangzhou 310012China China National Rice Research Institute Hangzhou 310006China Institute of Microbiology Chinese Academy of SciencesBeijing 100080China

Southern blot analysis indicated that mtID gene (encoding mannitol-1 -phosphate dehydrogenase) and gutD gene (encoding glucitol-6-phosphate dehydrogenase) had been integrated into the rice genome mediated by Agrobacterium tumefaciens LBA4404(pBIGM). The expression of the above two genes in transgenic rice plants was demonstrated by Northern blot analysis and enzymatic activity assay. Analysis of sugar alcohol showed that transgenic rice plants could produce and accumulate mannitol and sorbitol. The salt tolerance of transgenic plants was much higher than that of their controls.

关键词： Oryza sativa L. Agrobacterium tumefaciens D-mannitol-1-phosphate dehydrogenase D-glucitol-6-phosphate dehydrogenase salt tolerance.

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DcMFC-AnMBR耦合系统处理含磺胺嘧啶养猪废水的性能研究

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工业水处理 2024年第10期44卷 68-75页

作者：司马聪张新波程衍斌王慧中杜青钟玲玲 Huu Hao NGO 天津城建大学环境与市政工程学院基础设施防护和环境绿色生物技术国际联合研究中心天津300384 天津城建大学天津市水质科学与技术重点实验室天津300384

构建了双室微生物燃料电池-厌氧膜生物反应器(DcMFC-AnMBR)耦合工艺系统,探究了耦合系统处理含磺胺嘧啶(SDZ)养猪废水的产电、污染物去除及产气性能,同时分析了SDZ影响下膜污染特征变化。结果表明:1.0 mg/L SDZ存在下DcMFC-AnMBR耦合系... 详细信息

构建了双室微生物燃料电池-厌氧膜生物反应器(DcMFC-AnMBR)耦合工艺系统,探究了耦合系统处理含磺胺嘧啶(SDZ)养猪废水的产电、污染物去除及产气性能,同时分析了SDZ影响下膜污染特征变化。结果表明:1.0 mg/L SDZ存在下DcMFC-AnMBR耦合系统平均输出电压和库仑效率分别下降了6.11%和7%,耦合系统最大功率密度为35.34 mW/m^(2),COD去除率始终保持在98%,平均生物气产量为542.59 mL/d。同时,SDZ的存在使污泥混合液Zeta电位绝对值升高了25.83%,污泥平均粒径减小了14~16μm,可溶性微生物产物(SMP)和胞外聚合物(EPS)平均浓度分别上升了7.76%和35.43%。耦合工艺系统运行期间跨膜压差(TMP)始终在0附近,其污染缓解原因可能是电活性微生物对SMP和EPS进行了有效的氧化降解。

关键词：双室微生物燃料电池厌氧膜生物反应器养猪废水磺胺嘧啶膜污染

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Genome-wide identification, expression analysis of auxin-responsive GH_3 family genes in maize(Zea mays L.) under abiotic stresses

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Journal of Integrative Plant Biology 2015年第9期57卷 783-795页

作者： Shangguo Feng Runqing Yue Sun Tao Yanjun Yang Lei Zhang Mingfeng Xu huizhong wang Chenjia Shen College of Life and Environmental Sciences Hangzhou Normal University Henan Academy of Agricultural Sciences Department of Plant Pathology Washington State University

Auxin is involved in different aspects of plant growth and development by regulating the expression of auxin-responsive family genes. As one of the three major auxin-responsive families, GH3 (Gretchen Hagen3) genes participate in auxin homeostasis by catalyzing auxin conjugation and bounding free indole-3-acetic acid (IAA) to amino acids. However, how GH3 genes function in responses to abiotic stresses and various hormones in maize is largely unknown. Here, the latest updated maize (Zea mays L.) reference genome sequence was used to characterize and analyze the ZmGH3 family genes from maize. The results showed that 13 ZmGH3 genes were mapped on five maize chromosomes (total 10 chromosomes). Highly diversified gene structures and tissue-specific expression patterns suggested the possibility of function diversification for these genes in response to environmental stresses and hormone stimuli. The expression patterns of ZmGH3 genes are responsive to several abiotic stresses (salt, drought and cadmium) and major stress-related hormones (abscisic acid, salicylic acid and jasmonic acid). Various environmental factors suppress auxin free IAA contents in maize roots suggesting that these abiotic stresses and hormones might alter GH3-mediated auxin levels. The respon-siveness of ZmGH3 genes to a wide range of abiotic stresses and stress-related hormones suggested that ZmGH3s are involved in maize tolerance to environmental stresses.

关键词： Abiotic stress auxin auxin responsive gene GH3 gene family maize

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The linkage maps of Dendrobium species based on RAPD and SRAP markers

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Journal of Genetics and Genomics 2010年第3期37卷 197-204页

作者： Dawei Xue Shangguo Feng Hongyan Zhao Hua Jiang Bo Shen Nongnong Shi Jiangjie Lu Junjun Liu huizhong wang College o fLife and Environment Sciences Hangzhou Normal University Hangzhou 310036 China Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Science Hangzhou 310021 China

Dendrobium plants are used commonly as tonic herbs and health food in many Asian countries, especially in China. Here we report the genetic map construction of two Dendrobium species with a double pseudo-testcross strategy using random amplified polymorphic DNA （RAPD） and sequence-related amplified polymorphism （SRAP） markers. A F1 mapping population of 90 individuals was developed from a cross between D. officinale and D. hercoglossum. A total of 307 markers, including 209 RAPD and 98 SRAP, were identified and used for genetic linkage group （LG） analysis. The D. officinale linkage map consisted of 11 major linkage groups and 3 doublets, which covered 629.4 cM by a total of 62 markers with an average locus distance of 11.2 cM between two adjacent markers. The D. hercoglos- sum linkage map contained 112 markers mapped on 15 major and 4 minor linkage groups, spanning a total length of 1,304.6 cM with an average distance of I 1.6 cM between two adjacent markers. The maps constructed in this study covered 92.7% and 82.7% of the D. hercoglossum and D. officinale genomes respectively, providing an important basis for the mapping of horticultural and medicinal traits and for the application of marker-assisted selection in Dendrobium breeding program.

关键词： Dendrobium genetic linkage map RAPD SRAP

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食管癌新辅助治疗研究新进展

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食管疾病 2024年第2期6卷 107-114,123页

作者：银嘉鑫王慧中贾国霞全强赵建夫暨南大学第一附属医院广东广州510632

食管癌作为全球常见肿瘤之一,其发病率及死亡率较高,发现时多为中晚期,实现有效治疗目标仍具挑战性。当前新辅助治疗策略,包括化疗、放疗、分子靶向、免疫治疗及综合治疗,在临床中的价值得到广泛认可,但不同方案的选择存在一定争议。本... 详细信息

食管癌作为全球常见肿瘤之一,其发病率及死亡率较高,发现时多为中晚期,实现有效治疗目标仍具挑战性。当前新辅助治疗策略,包括化疗、放疗、分子靶向、免疫治疗及综合治疗,在临床中的价值得到广泛认可,但不同方案的选择存在一定争议。本文结合相关文献对食管癌新辅助治疗方法的研究现状及进展进行了总结和概述,并对其应用前景和存在的问题进行了分析,为进一步优化临床诊疗策略提供参考。

关键词：食管肿瘤新辅助治疗新辅助放化疗新辅助靶向/免疫治疗

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植物m^(6)A修饰对转录本调控及影响机制的研究进展

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杭州师范大学学报（自然科学版） 2024年第5期23卷 512-522页

作者：唐银鞠李绍蕾冯泽地王慧中孟一君杭州师范大学生命与环境科学学院浙江杭州311121 浙江省药用植物种质改良与质量控制技术重点实验室浙江杭州311121

近年来植物中mRNA m^(6)A修饰及其对靶mRNA的调控研究备受关注.高通量测序、比色法及液相色谱质谱联用技术的发展加深了对m^(6)A修饰的分析.转录组范围内通过甲基转移酶、去甲基化酶和结合蛋白对mRNA修饰形成m^(6)A选择性标记,调控转录... 详细信息

近年来植物中mRNA m^(6)A修饰及其对靶mRNA的调控研究备受关注.高通量测序、比色法及液相色谱质谱联用技术的发展加深了对m^(6)A修饰的分析.转录组范围内通过甲基转移酶、去甲基化酶和结合蛋白对mRNA修饰形成m^(6)A选择性标记,调控转录本选择性剪接、稳定、翻译和衰变.m^(6)A修饰水平的变化对于维持植物毛状体形成、开花时间、胁迫响应和光合作用等生命活动也具有重要作用.文章综述近年来mRNA m^(6)A甲基化修饰类型,以及植物m^(6)A修饰在分子遗传和植物生长发育中的研究进展,尤其关注植物中m^(6)A修饰对mRNA命运和表达调控的不同作用.

关键词： m^(6)A mRNA修饰表观转录组学转录调控

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