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Novel index for the prediction of significant liver fibrosis and cirrhosis in chronic hepatitis B patients in China

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World Journal of Gastroenterology 2022年第27期28卷 3503-3513页

作者： Min-Jun Liao Jun Li Wei Dang Dong-Bo Chen wan-ying qin Pu Chen Bi-Geng Zhao Li-ying Ren Ting-Feng Xu Hong-Song Chen Wei-Jia Liao Laboratory of Hepatobiliary and Pancreatic Surgery The Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology and Hepatology UnitNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Genetics and Precision Medicine Laboratory The Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease Peking University People’s HospitalBeijing 100044China

BACKGROUND Noninvasive,practical,and convenient means of detection for the prediction of liver fibrosis and cirrhosis in China are greatly *** To develop a precise noninvasive test to stage liver fibrosis and *** With liver biopsy as the gold standard,we established a new index,[alkaline phosphatase(U/L)+gamma-glutamyl transpeptidase(U/L)/platelet(109/L)(AGPR)],to predict liver fibrosis and *** addition,we compared the area under the receiver operating characteristic curve(AUROC)of AGPR,gammaglutamyl transpeptidase to platelet ratio,aspartate transaminase to platelet ratio index,and FIB-4 and evaluated the accuracy of these routine laboratory indices in predicting liver fibrosis and *** Correlation analysis revealed a significant positive correlation between AGPR and liver fibrosis stage(P<0.001).In the training cohort,the AUROC of AGPR was 0.83(95%CI:0.78-0.87)for predicting fibrosis(≥F2),0.84(95%CI:0.79-0.88)for predicting extensive fibrosis(≥F3),and 0.87(95%CI:0.83-0.91)for predicting cirrhosis(F4).In the validation cohort,the AUROCs of AGPR to predict≥F2,≥F3 and F4 were 0.83(95%CI:0.77-0.88),0.83(95%CI:0.77-0.89),and 0.84(95%CI:0.78-0.89),*** The AGPR index should become a new,simple,accurate,and noninvasive marker to predict liver fibrosis and cirrhosis in chronic hepatitis B patients.

关键词： Liver Fibrosis Cirrhosis Prediction Novel noninvasive marker Chronic hepatitis B

Radiomics model based on contrast-enhanced computed tomography to predict early recurrence in patients with hepatocellular carcinoma after radical resection

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World Journal of Gastroenterology 2023年第26期29卷 4186-4199页

作者： Shu-Qun Li Li-Li Su Ting-Feng Xu Li-ying Ren Dong-Bo Chen wan-ying qin Xuan-Zhi Yan Jia-Xing Fan Hong-Song Chen Wei-Jia Liao Laboratory of Hepatobiliary and Pancreatic Surgery The Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Department of Clinical Laboratory Nanxishan Hospital of Guangxi Zhuang Autonomous RegionGuilin 541002Guangxi Zhuang Autonomous RegionChina Peking University People’s Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseaseBeijing 100091China

BACKGROUND Radical resection remains an effective strategy for patients with hepatocellular carcinoma(HCC).Unfortunately,the postoperative early recurrence(recurrence within 2 years)rate is still *** To develop a radiomics model based on preoperative contrast-enhanced computed tomography(CECT)to evaluate early recurrence in HCC patients with a single *** We enrolled a total of 402 HCC patients from two centres who were diagnosed with a single tumour and underwent radical ***,the features from the portal venous and arterial phases of CECT were extracted based on the region of interest,and the early recurrence-related radiomics features were selected via the least absolute shrinkage and selection operator proportional hazards model(LASSO Cox)to determine radiomics scores for each ***,the clinicopathologic data were combined to develop a model to predict early recurrence by Cox ***,we evaluated the prediction performance of this model by multiple *** A total of 1915 radiomics features were extracted from CECT images,and 31 of them were used to determine the radiomics scores,which showed a significant difference between the early recurrence and nonearly recurrence *** and multivariate Cox regression analyses showed that radiomics scores and serum alphafetoprotein were independent indicators,and they were used to develop a combined model to predict early *** area under the receiver operating characteristic curve values for the training and validation cohorts were 0.77 and 0.74,respectively,while the C-indices were 0.712 and 0.674,*** calibration curves and decision curve analysis showed satisfactory accuracy and clinical ***-Meier curves based on recurrence-free survival and overall survival showed significant *** The preoperative radiomics model was shown to be effective for predicting early recurrence among HCC patients with a single tum

关键词： Hepatocellular carcinoma Contrast-enhanced computed tomography Radiomics Early recurrence

A Fluorescent Glucose Transport Assay for Screening SGLT2 Inhibitors in Endogenous SGLT2-Expressing HK-2 Cells

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Natural Products and Bioprospecting 2019年第1期9卷 13-21页

作者： Yan-Ting Lu Xiu-Li Ma Yu-Hui Xu Jing Hu Fang wang wan-ying qin Wen-Yong Xiong State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of the Chinese Academy of Sciences Beijing 100049China Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming 650201China

The sodium-dependent glucose transporters 2(SGLT2)plays important role in renal reabsorption of urinal glucose back to plasma for maintaining glucose *** approval of SGLT2 inhibitors for treatment of type 2 diabetes highlights the SGLT2 as a feasible and promising drug target in recent *** methods for screening SGLT2 inhibitors are complex,expensive and labor ***,these methods cannot directly measure nonradioactive glucose uptake in endogenous SGLT2-expressing kidney *** present work,human kidney cells,HK-2,was incubated with a fluorescent D-glucose derivant 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose(2-NBDG)and the fluorescent intensity of 2-NBDG was employed to measure the amount of glucose uptake into the *** optimizing the passages of HK-2 cells,2-NBDG concentration and incubation time,and by measuring glucose uptake treated by Dapagliflozin,a clinical drug of SGLT2 inhibitors,we successfully developed a new assay for measuring glucose uptake through *** nonradioactive microplate and microscope-based high-throughput screening assay for measuring glucose can be a new method for screening of SGLT2 inhibitors and implied for other cell assays for glucose measurement extensively.

关键词： SGLT2 inhibitor 2-NBDG HK-2 Glucoseuptake

Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest

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Natural Products and Bioprospecting 2020年第3期10卷 131-140页

作者： Rong-Fang Mu Yan-Fen Niu Qian wang Hui-Min Zhou Jing Hu wan-ying qin Wen-Yong Xiong State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanPeople’s Republic of China Key Laboratory of Medicinal Chemistry for Natural Resource Yunnan UniversityKunming 650500YunnanPeople’s Republic of China Biomedical Engineering Research Center Kunming Medical UniversityKunming 650500YunnanPeople’s Republic of China University of Chinese Academy of Sciences Beijing 100049People’s Republic of China

Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and ***,the function and mechanism of the compound in adipocyte differentiation is still *** we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets,triglycerides and the expressions of adipogenesis-related factors,including C/EBPβ,C/EBPα,PPARγ,and ***,we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation,most likely by suppress-ing mRNAs and proteins of CDK1,CDK2,Cyclin A and Cyclin ***,we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.

关键词： Eriocalyxin B Adipocyte differentiation Cell cycle

Adipogenesis Inhibitory Activity of Hypersampsone P from Hypericum subsessile

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Natural Products and Bioprospecting 2020年第3期10卷 163-170页

作者： Hui-Min Zhou Yan-Song Ye Na-Na Jiang Rong-Fang Mu Qian wang Jing Hu Xia Liu wan-ying qin Gang Xu Wen-Yong Xiong State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanPeople’s Republic of China University of Chinese Academy of Sciences Beijing 100049People’s Republic of China Department of Pharmacy Chongqing Traditional Chinese Medicine HospitalChongqing 400021China

Adamantane polycyclic polyprenylated acylphloroglucinols(PPAPs)with caged architecture,a special class of hybrid natural products,is specifically rich in the plant family Guttiferae,especially Hypericum or Garcinia *** P is one of Adamantane PPAPs compounds extracted from Hypericum *** we have chosen,screened ten PPAPs and identified one of them showed an activity in inhibiting of adipocytes ***,the compound,hypersamp-sone P,blunted the adipocyte differentiation ***,hypersampsone P down-regulated the expressions of several key regulators for adipogenesis,including PPARγand *** treatment of cells at the early stage of adipo-genesis by hypersampsone P induced the greatest blunting of adipocyte differentiation and the effect might be involved in the LKB1-AMPK signaling pathway.

关键词： Adipogenesis LKB1 AMPK Hypericum subsessile Hypersampsone P

Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng Zheng wang Zhou ying Mo Biao Zheng Miaomiao Han qin Zhong Wenwen Yang Peng Gao Lezhi Yang Jilei Liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater Bay Area Institute for Innovation Hunan University Changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the Na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more Na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced Na+storage performance.

关键词： X ray photoelectron spectroscopy

Nrf2/ARE通路与糖尿病相关动脉粥样硬化

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国际内分泌代谢杂志 2017年第3期37卷 203-206页

作者：缪莹万沁西南医科大学附属医院内分泌科泸州646000

氧化应激和炎性反应是糖尿病致动脉粥样硬化的两个主要危险因素,Nrf2/ARE通路是体内调控氧化应激的关键通路.激活Nrf2/ARE通路可保护血管内皮细胞、血管平滑肌细胞及胰岛β细胞免受氧化损伤,并可增加胰岛素分泌,改善胰岛素抵抗.Nrf2/AR... 详细信息

氧化应激和炎性反应是糖尿病致动脉粥样硬化的两个主要危险因素,Nrf2/ARE通路是体内调控氧化应激的关键通路.激活Nrf2/ARE通路可保护血管内皮细胞、血管平滑肌细胞及胰岛β细胞免受氧化损伤,并可增加胰岛素分泌,改善胰岛素抵抗.Nrf2/ARE通路对糖尿病动脉粥样硬化的防治具有重要意义.

关键词： Nrf2/ARE信号通路糖尿病动脉粥样硬化氧化应激

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Infiltration by monocytes of the central nervous system and its role in multiple sclerosis: reflections on therapeutic strategies

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Neural Regeneration Research 2025年第3期20卷 779-793页

作者： Guangyong Zhang qing Yao Chubing Long Pengcheng Yi Jiali Song Luojia Wu Wei wan Xiuqin Rao Yue Lin Gen Wei Jun ying Fuzhou Hua Department of Anesthesiology the Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Key Laboratory of Anesthesiology of Jiangxi Province NanchangJiangxi ProvinceChina

Mononuclear macrophage infiltration in the central nervous system is a prominent feature of neuroinflammation. Recent studies on the pathogenesis and progression of multiple sclerosis have highlighted the multiple roles of mononuclear macrophages in the neuroinflammatory process. Monocytes play a significant role in neuroinflammation, and managing neuroinflammation by manipulating peripheral monocytes stands out as an effective strategy for the treatment of multiple sclerosis, leading to improved patient outcomes. This review outlines the steps involved in the entry of myeloid monocytes into the central nervous system that are targets for effective intervention: the activation of bone marrow hematopoiesis, migration of monocytes in the blood, and penetration of the blood–brain barrier by monocytes. Finally, we summarize the different monocyte subpopulations and their effects on the central nervous system based on phenotypic differences. As activated microglia resemble monocyte-derived macrophages, it is important to accurately identify the role of monocyte-derived macrophages in disease. Depending on the roles played by monocyte-derived macrophages at different stages of the disease, several of these processes can be interrupted to limit neuroinflammation and improve patient prognosis. Here, we discuss possible strategies to target monocytes in neurological diseases, focusing on three key aspects of monocyte infiltration into the central nervous system, to provide new ideas for the treatment of neurodegenerative diseases.

关键词： blood–brain barrier macrophages monocytes multiple sclerosis neuroinflammation review therapy

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酶促重组等温扩增实时荧光法快速检测肺炎支原体方法的建立及应用

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生物技术通报 2022年第9期38卷 264-270页

作者：胡海洋应婉琴何军吕芷贤谢小平邓仲良南华大学衡阳医学院公共卫生学院卫生检验与检疫系衡阳421001 南华大学衡阳医学院附属南华医院衡阳421001 南华大学衡阳医学院附属第一医院衡阳421001

利用酶促重组等温扩增(enzymatic recombinase amplification,ERA)技术建立快速检测肺炎支原体的实时荧光检测方法。针对肺炎支原体P1基因设计特异性引物和探针,优化反应条件,分析其敏感性和特异性,并对临床样本进行验证。ERA实时荧光法... 详细信息

利用酶促重组等温扩增(enzymatic recombinase amplification,ERA)技术建立快速检测肺炎支原体的实时荧光检测方法。针对肺炎支原体P1基因设计特异性引物和探针,优化反应条件,分析其敏感性和特异性,并对临床样本进行验证。ERA实时荧光法在25-40℃均具有扩增能力,在35℃条件下对肺炎支原体的扩增效果最好,20 min内可完成扩增;该法对肺炎支原体的检出限为10^(3) copies/μL;并对其他6种呼吸道病原体进行检测,均无扩增曲线产生,有较好的特异性;以荧光定量PCR法检测结果为标准,ERA实时荧光法对34份临床样本的检测结果的诊断敏感度为96.15%、特异度为100%、阳性预测值为100%、阴性预测值为88.89%。本研究构建的ERA实时荧光法可以快速简单、灵敏和特异地检测出肺炎支原体,满足现场检测的需求。

关键词：酶促重组等温扩增技术实时荧光检测肺炎支原体

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