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Horticulture Research 2014年第1期1卷 142-149页

作者： wan-ping fang Lyndel W Meinhardt Hua-Wei Tan Lin Zhou Sue Mischke Dapeng Zhang College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Sustainable Perennial Crops Laboratory USDA-ARSBeltsville Agricultural Research CenterBeltsvilleMD 20705USA

Apart from water,tea is the world’s most widely consumed *** is produced in more than 50 countries with an annual production of approximately 4.7 million *** market segment for specialty tea has been expanding rapidly owing to increased demand,resulting in higher revenues and profits for tea growers and the *** varietal identification is critically important to ensure traceability and authentication of premium tea products,which in turn contribute to on-farm conservation of tea genetic *** a set of single nucleotide polymorphism(SNP)markers developed from the expressed sequence tag(EST)database of Camilla senensis,we genotyped deoxyribonucleic acid(DNA)samples extracted from a diverse group of tea varieties,including both fresh and processed commercial loose-leaf *** validation led to the designation of 60 SNPs that unambiguously identified all 40 tested tea varieties with high statistical rigor(pping protocol,effectively established and verified specific DNA fingerprints for all tested tea *** method provides a powerful tool for variety authentication and quality control for the tea *** is also highly useful for the management of tea genetic resources and breeding,where accurate and efficient genotype identification is essential.

关键词： revenue breeding expanding

Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) *** Using cDNA Amplified Fragment Length Polymorphism Technique

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： wan-ping fang~(1,2) Chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia wanG~(1,3) 1 Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036,China 2 College Of Horticulture,Nanjing Agriculture University,Nanjing 210095,China 3 Auhui Institute of Education,Hefei 230036,China KeyLaboratoryofTeaBiochemistryandBiotechnology MinistryofAgricultureAnhuiAgriculturalUniversityHefei230036.China CollegeOfHorticulture NanjingAgricultureUniversityNanjing210095China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea *** sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other *** complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation *** deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male *** named this completecDNA *** nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession *** Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 *** blot analysis was used to identify the temporalexpression of Tual in tea *** studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP Camellia sinensis flower bud α-tubulin Western blot pollen

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电泳涂料加热减量试验方法的探讨

现代涂料与涂装 2013年第7期16卷 50-51页

作者：于金鑫宛萍芳陈良印奇瑞汽车股份有限公司安徽芜湖241009

通过比对不同烘烤温度和试验样板对电泳漆加热减量试验结果的影响,提出测试电泳漆加热减量的烘烤温度应为(105±2)℃,时间为3 h,试验样板为冷轧板。

关键词：电泳漆加热减量磷化

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汽车常见腐蚀问题分析及改进措施探讨

环境技术 2010年第6期28卷 37-41页

作者：陈拯宛萍芳于磊王宏伟奇瑞汽车股份有限公司试验技术中心

汽车是耐用消费品,服役环境较恶劣,经常会发生腐蚀问题;腐蚀不仅影响汽车美观,而且还直接影响汽车质量和使用寿命。本文主要就汽车腐蚀类型及常见腐蚀问题进行总结分析,并提出预防改进措施。

关键词：汽车腐蚀分析改进措施

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油漆涂层老化黄变分析

环境技术 2013年第6期31卷 29-31,35页

作者：宛萍芳于金鑫王正陈良印奇瑞汽车股份有限公司芜湖241009

汽车涂装后形成的涂层,既要具备较好的外观装饰效果,又必须具有优良的防护性能,而在实际使用过程中,常因涂层老化而发生的黄变,严重影响了其原有的性能要求。文章分析了涂层老化黄变的原因,对比分析了几种面漆的耐老化性能,并提出提高... 详细信息

汽车涂装后形成的涂层,既要具备较好的外观装饰效果,又必须具有优良的防护性能,而在实际使用过程中,常因涂层老化而发生的黄变,严重影响了其原有的性能要求。文章分析了涂层老化黄变的原因,对比分析了几种面漆的耐老化性能,并提出提高涂层耐黄变性能的措施。

关键词：油漆涂层老化黄变

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电泳漆边角覆盖缺陷研究

现代涂料与涂装 2010年第4期13卷 55-56页

作者：于金鑫陈拯宛萍芳陈淑君奇瑞汽车股份有限公司试验技术中心安徽芜湖241009

介绍了检验电泳漆边角覆盖性的试验方法、电泳及烘干过程中尖端部位涂层的变化,对引起电泳漆边角部腐蚀的原因进行分析,列举了提高电泳漆边角覆盖性的途径。

关键词：电泳涂装边角覆盖表面张力

BIFURCATION AND SPATIOTEMPORAL PATTERNS IN A HOMOGENEOUS DIFFUSION-COMPETITION SYSTEM WITH DELAYS

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International Journal of Biomathematics 2012年第6期5卷 19-41页

作者： JIA-fang ZHANG wan-TONG LI XIANG-ping YAN School of Mathematics and Information Sciences Henan University Kaifeng Henan 575001 P. R. China School of Mathematics and StatisticsLanzhou University Lanzhou Gansu 730000 P. R. China Department of MathematicsLanzhou Jiaotong University Lanzhou Gansu 730070 P. R. China

A competitive LotkaVolterra reactiondiffusion system with two delays subject to Neumann boundary conditions is considered. It is well known that the positive con stant steady state of the system is globally asymptotically stable if the interspecies competition is weaker than the intraspecies one and is unstable if the interspecies com petition dominates over the intraspecies one. If the latter holds, then we show that Hopf bifurcation can occur as the parameters （delays） in the system cross some critical val ues. In particular, we prove that these Hopf bifurcations are all spatially homogeneous if the diffusive rates are suitably large, which has the same properties as Hopf bifur cation of the corresponding delayed system without diffusion. However, if the diffusive rates are suitably small, then the system generates the spatially nonhomogeneous Hopf bifurcation. Furthermore, we derive conditions for determining the direction of spatially nonhomogeneous Hopf bifurcations and the stability of bifurcating periodic solutions. These results indicate that the diffusion plays an important role for deriving the complex spatiotemporal dynamics.

关键词： LotKa-Volterra competition system time delay spatial diffusion Hopf bifur-cation periodic solution.

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装饰性塑料电镀件CASS试验问题分析

环境技术 2011年第1期29卷 21-24页

作者：宛萍芳陈拯王宏伟于磊于金鑫奇瑞汽车股份有限公司试验技术中心

汽车用塑料电镀件是一种防护——装饰性镀层,既要具备较好的外观装饰效果,又必须具有优良的防护性能,CASS试验是其性能测试的重要项目,本文就塑料电镀件在CASS试验中出现的各种腐蚀缺陷形态进行了总结,分析了抗腐蚀性下降的原因。

关键词：塑料件电镀 CASS试验腐蚀

Plastic limit load analysis for pressure pipe with incomplete welding defects based on the extended Net Section Collapse Criteria

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2010年第6期11卷 440-448页

作者： Zhi-jiang JIN Cheng-hang JIANG Xian-ping wan Po CHEN Xiao-fang wanG Institute of Chemical Process Equipment Zhejiang University Hangzhou 310027 China

With von Mises yield criterion,the loading range of Net Section Collapse(NSC) Criteria is extended from combined tension and bending loadings to combined bending,torsion and internal pressure loadings.A new theoretical analyzing method of plastic limit load for pressure pipe with incomplete welding defects based on the extended NSC Criteria is presented and the correlative formulas are deduced,the influences of pipe curvature,circumferential length and depth of incomplete welding defects on the plastic limit load of pressure pipe are considered as well in this ***,according to the orthogonal experimental design method,the plastic limit loads are calculated by the finite element method and compared with the theoretical *** results show that the expressions of plastic limit load of pressure pipe with incomplete welding defects under bending,torsion and internal pressure based on extended NSC criteria are *** study provides an important theoretical basis for the establishment of safety assessment measure towards pressure pipe with incomplete welding defects.

关键词： Net Section Collapse(NSC) Criteria Incomplete welding defects Pressure pipe Plastic limit load Finite element

Relationship Between the Strengthening Effect and the Morphology of Precipitates in Al–7.4Zn–1.7Mg–2.0Cu Alloy

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Acta Metallurgica Sinica(English Letters) 2014年第6期27卷 1070-1077页

作者： Xiubo Yang Jizi Liu Jianghua Chen Caiyun wan Lei fang ping Liu Cuilan Wu College of Materials Science & Engineering Hunan University Hunan Province Key Laboratory for Spray Deposition Technology and Application Hunan University

The morphological evolution of the precipitates in Al–7.4Zn–1.7Mg–2.0Cu（wt%） alloy was studied by highresolution transmission electron microscopy（HRTEM）. Statistics reveal that the hardness of the alloy changes accordingly with the change of the average thickness–diameter ratio of precipitates. The GPII zones are mainly responsible for the first and also the highest hardness peak. They grow in diameter and keep 7-atomic-layer in thickness. Once the thickness changes, the phase transformation from GPII zone to g0 or g-precursor would occur. The resultant metastable g0 and g-precursor precipitates grow in both diameter and thickness, but much faster in the former. After the first hardness peak,the metastable g0 precipitates and g-precursor, coexisting with part of GPII zones, are counted as the main hardening precipitates.

关键词： Aluminum alloys Precipitates Age hardening Electron microscopy Morphology