Varietal identification of tea(Camellia sinensis)using nanofluidic array of single nucleotide polymorphism(SNP)markers
使用单核苷酸多态性(SNP)标记纳流控阵品种鉴定的茶(茶树)作者机构:College of HorticultureNanjing Agricultural UniversityNanjing 210095China Sustainable Perennial Crops LaboratoryUSDA-ARSBeltsville Agricultural Research CenterBeltsvilleMD 20705USA
出 版 物:《Horticulture Research》 (园艺研究(英文))
年 卷 期:2014年第1卷第1期
页 面:142-149页
核心收录:
学科分类:0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学]
基 金:This work was partially supported by the National Natural Science Foundation of China(30800884,31370688) References to a company and/or product by the USDA are only for the purposes of information and do not imply approval or recommendation of the product to the exclusion of others that may also be suitable
主 题:revenue breeding expanding
摘 要:Apart from water,tea is the world’s most widely consumed *** is produced in more than 50 countries with an annual production of approximately 4.7 million *** market segment for specialty tea has been expanding rapidly owing to increased demand,resulting in higher revenues and profits for tea growers and the *** varietal identification is critically important to ensure traceability and authentication of premium tea products,which in turn contribute to on-farm conservation of tea genetic *** a set of single nucleotide polymorphism(SNP)markers developed from the expressed sequence tag(EST)database of Camilla senensis,we genotyped deoxyribonucleic acid(DNA)samples extracted from a diverse group of tea varieties,including both fresh and processed commercial loose-leaf *** validation led to the designation of 60 SNPs that unambiguously identified all 40 tested tea varieties with high statistical rigor(p0.0001).Varietal authenticity and genetic relationships among the analyzed cultivars were further characterized by ordination and Bayesian clustering *** SNP markers,in combination with a high-throughput genotyping protocol,effectively established and verified specific DNA fingerprints for all tested tea *** method provides a powerful tool for variety authentication and quality control for the tea *** is also highly useful for the management of tea genetic resources and breeding,where accurate and efficient genotype identification is essential.