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Antioxidative Effect of Luteolin Pretreatment on Simulated Ischemia/Reperfusion Injury in Cardiomyocyte and Perfused Rat Heart

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Chinese Journal of Integrative Medicine 2017年第7期23卷 518-527页

作者： ZHANG rui-qu li Dong-ye XU tong-da ZHU Sha-sha PAN Huan-jun FANG Fang WU Xin sun hong Institute of Cardiovascular Disease Research Xuzhou Medical College Xuzhou Jiangsu Province 221002 China Department of Physiology Xuzhou Medical College XuzhouJiangsu Province 221002 China

Objective：To investigate the antioxidative effect and mechanism of luteolin on rat cardiomyocytes and isolated hearts fol owed by simulated ischemia/reperfusion（SI/R） injury. Methods：The left ventricular cardiomyocytes and the isolated hearts from adult rats were subjected to SI/R injury. The experiment groups included control, SI/R, luteolin ＋ SI/R（Lut ＋ SI/R）, vitamin E（Vit E）＋ SI/R, and LY294002 ＋ luteolin ＋ SI/R（LY ＋ Lut ＋ SI/R） groups. Cell viability, shortening amplitude, lactate dehydrogenase（LDH） release, superoxide dismutase（SOD） activity, the production of reactive oxygen species（ROS） and malondialdehyde（MDA）, expression levels of Akt, phosphorylated Akt, NOX2（gp91phox）, NOX2 m RNA, mitogen-activated protein kinase（p38 MAPK） and phosphorylated p38 MAPK were al measured after 3-h simulated ischemia and 2-h simulated reperfusion procedure in cardiomyocytes. Vit E was used as a standard control. The contractile function of isolated hearts was further observed after they were subjected to 30-min global ischemia and 120-min reperfusion. Results：Pretreatment with 8-μmol/L luteolin substantially increased cel viability and shortening amplitude, while reducing evidence of oxidative stress-induced damage in the cel s. In addition, the expression of NOX2, NOX2 m RNA and phosphorylation of p38 MAPK were al downregulated. Furthermore, pretreatment with 40-μmol/L luteolin improved the recovery of myocardial contractile function fol owing SI/R-induced injury, and luteolin markedly increased phosphorylation of Akt. However, all of the above effects were partially inhibited by the phosphatidylinositol 3-kinase（PI3K） inhibitor, LY294002. Conclusions：Luteolin prevents SI/R-induced myocardial damage by reducing oxidative stress-induced injury in isolated rat hearts and cardiomyocytes, and the cardioprotection induced by luteolin was partial y mediated by the PI3K/Akt pathway.

关键词： luteolin cardiomyocyte ischemia/reperfusion cardioprotection

An Indoor Environment Sensing and Localization System via mmWave Phased Array

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Journal of Communications and Information Networks 2022年第4期7卷 383-393页

作者： Yifei sun Jie li tong Zhang rui wang Xiaohui Peng Xiao Han Haisheng Tan Department of Electronic and Electrical Engineering Southern University of Science and TechnologyShenzhen 518055China Department of Computer Science the University of Hong KongHong Kong 999077China Huawei Technologies Co. Ltd.Shenzhen 518129China liNKE Lab School of Computer Science and Technologythe University of Science and Technology of ChinaHefei 230026China

In this paper,an indoor layout sensing and localization system with testbed in the 60-GHz millimeter wave(mmWave)band,named mmReality,is *** mmReality system consists of one transmitter and one mobile receiver,both with a phased array and a single radio frequency(RF)*** reconstruct the room layout,the pilot signal is delivered from the transmitter to the receiver via different pairs of transmission and receiving beams,so that multipath signals in all directions can be *** spatial smoothing and the two-dimensional multiple signal classification(MUSIC)algorithm are applied to detect the angle-of-departures(AoDs)and angle-of-arrivals(AoAs)of propagation ***,the technique of multi-carrier ranging is adopted to measure the path ***,with the measurements of the receiver in different locations of the room,the receiver and virtual transmitters can be pinpointed to reconstruct the room *** show that the reconstructed room layout can be utilized to localize a mobile device via the AoA spectrum.

关键词： millimeter wave indoor sensing and localization MUSIC algorithm multi-input multi output(MIMO) room layout integrated sensing and communication(ISAC) environment sensing orthogonal frequency division multiplexing(OFDM)

Polycystic Ovary Syndrome is not Associated with Offspring Birth Weight: A Mendelian Randomization Study

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Biomedical and Environmental Sciences 2021年第2期34卷 170-174页

作者： WU Peng Fei li rui Zhuo ZHANG wan SU Zhong Zhen liN Yu hong School of life Sciences Central South UniversityChangsha 410008HunanChina Department of Neurology Beth Israel Deaconess Medical CenterBoston 02115MAUSA Department of Ultrasonography Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000GuangdongChina Department of Biology Boston UniversityBoston 02215MAUSA

Polycystic ovary syndrome(PCOS) is a common metabolic and hormonal disorder afflicting approximately 5%–20% of all women of reproductive age~([1]). PCOS is characterized with hyperandrogenism,oligo-anovulation, and a polycystic ovarian morphology. The syndrome features heterogeneous manifestations, such as hirsutism, menstrual dysfunction, and obesity. Women with PCOS are at higher risk of developing multiple metabolic comorbidities and subsequent cardiovascular complications even beyond childbearing age.

关键词： cardiovascular cystic obesity

Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses

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Current Medical Science 2020年第4期40卷 614-617页

作者： Ze-gang WU hong-yun ZHENG Jian GU Feng li rui-long LV Ya-yun DENG wan-zhou XU Yong-qing tong Department of Clinical Laboratory Rentnin Hospital of Wuhan UniversityWuhan 430060China

Summary:The novel coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan,Hubei province of China in January *** study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for *** patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13,2020 and throat swabs were *** swabs were stored at room tempcrature(20-25℃),then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses(56℃for 30,45,60 min;65,70,80℃for 10,15,20 min).Control aliquots were stored at room temperature for 60 *** all aliquots were tested in a real-time fluorescence PCR using primers against *** of inactivation temperature and time,7 of 12 cases(58.3%)tested were positive for SARS-CoV-2 by PCR,and cycle threshold values were *** results suggest that virus inactivation parameters exert minimal infuence on PCR test *** at 65℃for 10 min may be sufficient to ensure safe,reliable testing.

关键词： SARS-CoV-2 COVID-19 throat swabs real-time fluorescence PCR

Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L.

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第2期12卷 135-142页

作者： Jie GAO Cui-rong sun Jie-hong YANG Jian-mei SHI Yue-guang DU Yu-yan ZHANG Jin-hui li Hai-tong wan Department of Chemistry Zhejiang University Hangzhou 310027 China Institute of Cardio-Cerebrovascular Disease Zhejiang Chinese Medical University Hangzhou 310053 China

The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. （RPL）, a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepatoprotective effects against carbon tetrachloride （CCI4）-induced hepatic injury in mice, which was at least partially attributed to its strong antioxidant capacity. Treatment with RPL extract markedly attenuated the increases in serum alanine ami- notransferase （ALT） and aspartate aminotransferase （AST） levels caused by CCI4 intoxication. It also significantly prevented the decrease in superoxide dismutase （SOD） activity and the increase in malondialdehyde （MDA） content of liver tissue. Meanwhile, histopathological changes of hepatic damage were also remarkably ameliorated. Phytochemical analysis based on high-performance liquid chromatography/tandem mass spectrometry （HPLC-MS/MS） revealed the presence of various phenolic compounds, including caffeic acid conjugates, ellagic acid glycosides, and flavonol glycosides, which might be responsible for the hepatoprotective and antioxidant activities of RPL.

关键词： Rubus parvifofius L. High-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) Hepatoprotective activity Antioxidant activity

Protective effects of organic extracts of Alpinia oxyphylla against hydrogen peroxide-induced cvtotoxicitv in PC12 cells

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Neural Regeneration Research 2020年第4期15卷 682-689页

作者： li-hong Duan Meng li Chun-Bao wang Qing-Mei wang Quan-Quan liu wan-Feng Shang Ya-Jin Shen Zhuo-Hua lin tong-Yang sun Zheng-Zhi Wu Ying-hong li Yu-Long wang Xun Luo Shenzhen Institute of Geriatrics ShenzhenGuangdong ProvinceChina Department of Rehabilitation the Second People’s Hospital of ShenzhenShenzhenGuangdong ProvinceChina Stroke Biological Recovery Laboratory Spaulding Rehabilitation HospitalHarvard Medical SchoolCharlestownMAUSA Department of Mechanical and Biomedical Engineering City University of Hong KongHong Kong Special Administrative RegionChina Department of Rehabilitation the First Affiliated Hospital of Shenzhen UniversityShenzhenGuangdong ProvinceChina School of Mechanical and Automotive Engineering South China University of TechnologyGuangzhouGuangdong ProvinceChina School of Mechanical Engineering Guangxi University of Science and TechnologyLiuzhouGuangxi Zhuang Autonomous RegionChina Mingkai Smart Medical Robot Co. Ltd.ShenzhenGuangdong ProvinceChina Kerry Rehabilitation Medicine Research Institute ShenzhenGuangdong ProvinceChina

Alpinia oxyphylla,a traditional herb,is widely used for its neuroprotective,antioxidant and memory-improving ***,the neuroprotective mechanisms of action of its active ingredients are *** this study,we investigated the neuroprotective effects of various organic extracts of Alpinia oxyphylla on PC12 cells exposed to hydrogen peroxide-induced oxidative injury in *** oxyphylla was extracted three times with 95%ethanol(representing extracts 1–3).The third 95%ethanol extract was dried and resuspended in water,and then extracted successively with petroleum ether,ethyl acetate and n-butanol(representing extracts 4–6).The cell counting kit-8 assay and microscopy were used to evaluate cell viability and observe the morphology of PC12 *** protective effect of the three ethanol extracts(at tested concentrations of 50,100 and 200μg/mL)against cytotoxicity to PC12 cells increased in a concentration-dependent *** ethyl acetate,petroleum ether and n-butanol extracts(each tested at 100,150 and 200μg/mL)had neuroprotective effects as *** optimum effective concentration ranged from 50–200μg/mL,and the protective effect of the ethyl acetate extract was comparatively *** results demonstrate that organic extracts of Alpinia oxyphylla protect PC12 cells against apoptosis induced by hydrogen *** findings should help identify the bioactive neuroprotective components in Alpinia oxyphylla.

关键词： active ingredients Alpinia oxyphylla apoptosis ethanol crude extract fraction hydrogen peroxide nerve regeneration neuroprotective agent neuroprotective effects PC12 cells traditional herb

Evaluating modified effects of family factors on intervention to protect primary school students from the impact of heatwave

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Advances in Climate Change Research 2023年第6期14卷 866-872页

作者： rui ZHANG Bo sun Si-Yuan WU Shui-ling QU Yan wanG Yu-Jie MENG Song-wang wanG Xiao-Yuan YAO Shilu tong Yong-hong li Yi-Bin CHENG Chinese Center for Disease Control and Prevention Bejing 102206China National Institute of Environmental Health Chinese Center for Disease Control and PreventionBeijing 100021China China CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China Sprott School of Businss Carleton UniversityOttawa Ontario KIS 5B6Canada School of Public Health and Social Work Queensland University of Technology BrisbaneBrisbane 4006Australia

Identifying the factors that may impact intervention effectiveness of primary students is crucial for developing targeted intervention strategies on heatwave *** purpose of the study was to assess how the family factors could affect the intervention effects of primary school students to adapt heatwave,using the data from a previously conducted primary school-based intervention *** study recruited 648 students(9-13 years old)and their parents from two primary schools in Dongtai of Jiangsu province,*** May to September 2017,a 5-mon intervention for enhancing knowledge,attitude and practice(KAP)was implemented at the intervention *** students'KAP scores and the family-related information were measured by designed self-administered questionnaires at intervention school and control school separately before and after *** effects of the intervention were explored through the propensity score matching and difference-in-difference method(PSM-DID),and stratifed analysis were conducted by family *** overall KAP score increased by 2.391(95%CI:1.881,2.902)after intervention,which shows that the intervention was *** students whose parents with higher school educational attainment,parents with employment,and household number≤3 achieved significantly higher increment of KAP scores than their *** students whose parents in marriage achieved higher score increasing than parents not in marriage,the difference was *** findings indicated that parental education status,working status and the household size were the influence factors of heatwave adaption interventions for primary school *** interventions should take parental education status,working status and household size into account to improve the effectiveness of heatwave adaption intervention for primary school students.

关键词： Heatwave Intervention Family factors Primary students Parents

Space life Science of China

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空间科学学报 2018年第5期38卷 820-828页

作者： MA hong REN Hao li Xiaoqiong YANG Chunhua CHEN Yu wanG rui YING Zhang wanG Changyong ZHOU Jin li hong JIANG Xiaoxia ZHANG Fengzhi HAO tong ZHU Huimin DONG Xiaohui ZHENG Huiqiong wanG lihua sun Weining tong Guanghui ZHENG Weibo ZHANG Tao LONG Mian liU hong ZHUANG Fengyuan DENG Yulin School of life Science Beijing Institute of Technology Institute of Military Cognitive and Brain Sciences Academy of Military Medical Sciences Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and EcologyChinese Academy of Sciences Shanghai Institute of Technical Physics Chinese Academy of Sciences National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences School of Biological Science and Medical Engineering Beihang University

With the human space exploration activities, space life science is an emerging interdiscipline,which covers a wide range of researches. Based on our country's manned space station and recoverable satellite science experimental platform, the development of space life science research is very important to acquire new knowledge or new technological innovation, to give further services to the human space exploration activities,to improve the national economic and social development. Both ground-based and flight applied studies were continuously performed in the previous 2 years. Here, we review and summarize the researches on space life sciences contributed by Chinese scientists.

关键词：空间生命科学中国科学家探索活动科学研究国家经济太空站试验性开发

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SysFinder：A customized platform for search,comparison and assisted design of appropriate animal models based on systematic similarity

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Journal of Genetics and Genomics 2017年第5期44卷 251-258页

作者： Shuang Yang Guoqing Zhang wan liu Zhen wang Jifeng Zhang Dongshan Yang Y.Eugene Chen hong sun Yixue li Department of Bioinformatics and Biostatistics School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China Shanghai Center for Bioinformation Technology Shanghai 200235 China Key Lab of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Center for Advanced Models for Translational Sciences and Therapeutics University of Michigan Medical Center Ann Arbor M148109 USA Biomedical Information Research Center Children's Hospital of Shanghai Shanghai 200040 China Collaborative Innovation Center for Genetics and Development Fudan University Shanghai 200433 China

Animal models are increasingly gaining values by cross-comparisons of response or resistance to clinical agents used for ***,many disease mechanisms and drug effects generated from animal models are not transferable to *** address these issues,we developed SysFinder（http：//***/SysFinder）,a platform for scientists to find appropriate animal models for translational *** offers a ＂topic-centered＂ approach for systematic comparisons of human genes,whose functions are involved in a specific scientific topic,to the corresponding homologous genes of animal *** topic can be a certain disease,drug,gene function or biological *** calculates multi-level similarity indexes to evaluate the similarities between human and animal models in specified scientific ***,SysFinder offers species-specific information to investigate the differences in molecular mechanisms between humans and animal ***,SysFinder provides a userfriendly platform for determination of short guide RNAs（sgRNAs） and homology arms to design a new animal *** studies illustrate the ability of SysFinder in helping experimental *** is a useful platform for experimental scientists to carry out their research in the human molecular mechanisms.

关键词： Database Animal models Function similarity