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Advances in Bioscience and Biotechnology 2013年第12期4卷 1079-1085页

作者： Rahayu Ahmad Najeeb Kaid Nasser Al-Shorgani Aidil Abdul Hamid wan mohtar wan yusoff Fauzi Daud School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Selangor Malaysia School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Selangor Malaysia Department of Applied Microbiology Faculty of Applied Sciences Taiz University Taiz Yemen

The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81;sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

关键词： Lentinus squarrosulus Response Surface Methodology (RSM) Exopolysaccharide Submerged Culture Central Composite Design (CCD)

Antioxidant Properties and Glucan Compositions of Various Crude Extract from Lentinus squarrosulusMycelial Culture

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Advances in Bioscience and Biotechnology 2014年第10期5卷 805-814页

作者： Rahayu Ahmad Sutha Muniandy Nur Iffah Abdullah Shukri Siti Mariatul Usyida Alias Aidil Abdul Hamid wan mohtar wan yusoff Sahidan Senafi Fauzi Daud School of Bioscience and Biotechnology Faculty Science and Technology National University of Malaysia Bangi Malaysia

Various crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth were prepared for antioxidant studies, glucan and carbohydrate compound analysis. Crude extract from ethanol, cold and hot water extraction was tested on several complementary test systems namely DPPH free radical scavenging, β-carotene linoleic acid oxidation, total phenolic compound, total flavonoid compound, total carbohydrate content and total glucan compositions. The antioxidant activity of the crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth was determined by 2,2’ diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and β-carotene linoleic acid assay. Crude extracted from culture broth EPS EE recorded the highest radical scavenging effects at 20 mg/mL (85%) which is closer to the control antioxidants (ascorbic acid 97%;BHT 92.4%;BHA 93.6%). Both the ethanol extract (IPS EE and EPS EE) exhibited the highest β-carotene linoleic acid oxidation by recording IC50 values of 0.6 and 0.65 mg/mL respectively. IPS EE (0.59 mg/g) and EPS WE (0.58 mg/g) showed high flavonoid content whereas total phenol content was high in EPS WE (0.85 mg/g) and IPS EE (0.84 mg/g). Total carbohydrate concentrations were high in IPS HWE and IPS CWE with 19.1 and 16.3 mg/mL respectively and these data correlate with the β-glucan content in both extracts. The highest β-glucan content found in IPS CWE and IPS HWE were 12.2 and 11.4% w/w respectively.

关键词： Lentinus squarrosulus β-Glucan Antioxidant Polysaccharides Submerged Fermentation

Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of Pleurotus flabellatusUsing Response Surface Methodology

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Advances in Bioscience and Biotechnology 2015年第6期6卷 419-426页

作者： Shaiful Azuar Mohamad Mat Rasol Awang Rosnani Abdul Rashid Liew Siew Ling Fauzi Daud Aidil Abdul Hamid Rahayu Ahmad wan mohtar wan yusoff Agrotechnology and Biosciences Division Malaysian Nuclear Agency Bangi Malaysia Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Malaysia

The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. The central composite design was applied to study the significant factors and the interactions between the chosen factors, if present. The Design Expert software generated 20 runs. The optimized conditions obtained were as follows: the agitation rate of 129.8 rpm, incubation temperature at 27.8°C, and initial pH of 6.06. The optimized conditions tripled the productivity at the range of 980 - 1040 mg/litre/day compared to the initial rate productivity at 310 mg/litre/day. From the quadratic equation,the agitation rate, temperature and the interaction between agitation rate and temperature were found to be significant (p < 0.05). At optimum conditions, the experimental data supported the theoretical estimate.

关键词： Submerged Culture Fermentation Response Surface Methodology Mushroom Production

Production of Endopolysaccharides from Malaysia’s Local Mushrooms in Air-Lift Bioreactor

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Advances in Bioscience and Biotechnology 2015年第7期6卷 456-462页

作者： Shaiful Azuar Mohamad Mat Rasol Awang Rusli Ibrahim Choong Yew Keong Mohd Yusof Hamzah Rosnani Abdul Rashid Sobri Hussein Khairuddin Abdul Rahim Fauzi Daud Aidil Abdul Hamid wan mohtar wan yusoff Agrotechnology and Biosciences Division Malaysian Nuclear Agency Bangi Malaysia Herbal Medicine Research Centre Institute For Medical Research Kuala Lumpur Malaysia Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Malaysia

Four local mushroom species, viz. Auricularis polytricha, Lentinus edodes, Agrocybe sp and Pleurotus flabellatus were grown under submerged culture and screened for endopolysaccharides. The fermentation was done in 250 ml working volume Erlenmeyer flask and the fermentation curves for all species were established. Pleurotus flabellatus has the highest rate of biomass production at the rate of 0.180 g/L/day, at 10 days hence chosen for further investigation. Two additional media, viz. Mushroom Complete Media (MCM) and Yeast Malt (YM) were selected to be compared with potato extract(PE) media used initially. MCM media produced the highest biomass productivity at the rate of 0.311 g/L/day. Pleurotus flabellatus biomass was extracted using modified Mizuno method and the endopolysaccharide obtained was tested for β-glucan. The yield of β-glucan was 7.70 ± 1.11 g/100g. The polysaccharides were purified using column chromatography to yield four fractions. The fourth fraction F4, gave the highest molecular weight at 3.058 × 106 Dalton (11.8%) and 1.282 × 104 Dalton (88.2%). The mushroom, P. flabbelatus was cultured using air-lift bioreactor, and the highest productivity was obtained at air-flowrate 2 L/min, yielding 2.25 g/L/day. The yield of biomass against substrate used (glucose consumption) Yb/s was 0.78 g/g.

关键词： Submerged Culture Fermentation Mushroom β-Glucan Column Chromatography Molecular Weight

Nutritional and therapeutic benefits of coconut milk and its potential as a plant-based functional yogurt alternative: a review

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Food Science and Human Wellness 2024年

作者： Gengghatarani Gengan Nurul Solehah Mohd Zaini Nazamid Saari Anis Shobirin Meor Hussin Ahmad Haniff Jaafar Hanan Hasan Elicia Jitming Lim wan Abd Al Qadr Imad wan-mohtar Muhamad Hafiz Abd Rahim Functional Omics and Bioprocess Development Laboratory， Institute of Biological Sciences， Faculty of Science， Universiti Malaya Faculty of Food Science and Technology， Universiti Putra Malaysia School of Life and Environmental Sciences， The University of Sydney

Plant-based milks are on the rise due to an increased awareness of their sustainability and health benefits. Currently， dairy milk is the most nutritionally complete beverage， but it suffers from the presence of indigestible lactose and allergenic proteins. Coconut milk has been around for a long time， but its application is limited due to a perceived lack of specific nutrients， high saturated fat levels， and low acceptability. Recent evidence indicates， however， that the saturated fat and other plant-based components found in coconut milk are good for metabolic outcomes and brain health. The conversion of coconut milk to yoghurt will further improve its functionality by boosting its existing nutritional qualities. In this article， the nutritional value of coconut milk， as well as its potential downsides， its application as yoghurt， and suggestions for enhancing its nutritional functionality will be examined.

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Oscillating-grid turbulence at large strokes: Revisiting the equation of Hopfinger and Toly

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Journal of Hydrodynamics 2016年第3期28卷 473-481页

作者： wan hanna melini wan mohtar Department of Civil and Structural Engineering Faculty Engineering and Built Environment Universiti Kebangsaan Malaysia Bandar BaruBangi 43600 Selangor Malaysia

A quasi-isotropic, quasi-homogeneous turbulence generated by an oscillating-grid, spatially decays according to power law of u ∝ Z-nM , where u is the root mean square （rms） horizontal velocity, Z is the vertical distance from the grid and nu = 1. However, the findings of Nokes and Yi indicate that as the stroke of oscillation increases, the power law n u= 1 and does not follow the established decay law equation of Hopfinger. This paper investigates the characteristics of the turbulence that are generated using larger strokes S/M = 1.6 and 2 and compares with that obtained using a S/M = 0.8, which is the stroke used when the equation was developed. Measurements of the grid-generated turbulence in a water tank were taken using particle image velocimetry （PIV）. The results showed that the homogeneity occurred at distance beyond 2.5 mesh spacings away from the grid midplane, independent of the stroke and the frequency of oscillation. Within this region, the turbulent kinetic energy distribution was quasi-homogeneous, and the secondary mean flow is negligible. The statistical characteristics of the measured turbulence confirmed that although nu decreases as stroke increases, the grid-turbulence generated at S/M = 1.6 and 2 obeys the universal decay law.

关键词： oscillating-grid turbulence large strokes decay power law

Ensuring water security by utilizing roof-harvested rainwater and lake water treated with a low-cost integrated adsorption-filtration system

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Water Science and Engineering 2017年第2期10卷 115-124页

作者： Riffat Shaheed wan Hanna Melini wan mohtar Ahmed El-Shafie Department of Civil and Structural Engineering Faculty of Engineering and Built Environment Universiti Kebangsaan MalaysiaBandar Baru Bangi 43600 Malaysia

Drinking water is supplied through a centralized water supply system and may not be accessed by communities in rural areas of *** study investigated the performance of a low-cost, self-prepared combined activated carbon and sand filtration(CACSF) system for roofharvested rainwater and lake water for potable use. Activated carbon was self-prepared using locally sourced coconut shell and was activated using commonly available salt rather than a high-tech procedure that requires a chemical reagent. The filtration chamber was comprised of local,readily available sand. The experiments were conducted with varying antecedent dry intervals(ADIs) of up to 15 d and lake water with varying initial chemical oxygen demand(COD) concentration. The CACSF system managed to produce effluents complying with the drinking water standards for the parameters p H, dissolved oxygen(DO), biochemical oxygen demand(BOD5), COD, total suspended solids(TSS), and ammonia nitrogen(NH_3-N). The CACSF system successfully decreased the population of Escherichia coli(E. coli) in the influents to less than 30 CFU/m L. Samples with a higher population of E. coli(that is, greater than 30 CFU/m L) did not show 100% removal. The system also showed high potential as an alternative for treated drinking water for roof-harvested rainwater and class II lake water.

关键词： Low-cost activated carbon Integrated adsorption-sand filtration Roof-harvested rainwater Lake water Water security

通过振荡网格实验研究初始泥沙运动和沉积物再悬浮（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2017年第11期18卷 882-894页

作者： wan Hanna Melini wan mohtar Department of Civil & Structural Engineering University Kebangsaan Malaysia

本文系统地研究了振荡网格生成的湍流效应对初始泥沙运动和沉积物再悬浮的影响,总结了影响泥沙运动和沉积的因素,给出了沉积物在宏观角度移动的基本概念和观点,并详细讨论了湍流对于沉降速度的影响。结果表明,湍流扰动对初始泥沙运动有... 详细信息

本文系统地研究了振荡网格生成的湍流效应对初始泥沙运动和沉积物再悬浮的影响,总结了影响泥沙运动和沉积的因素,给出了沉积物在宏观角度移动的基本概念和观点,并详细讨论了湍流对于沉降速度的影响。结果表明,湍流扰动对初始泥沙运动有重要影响。悬浮层内的浓度分布与沉积物特征和湍流结构有关。粒子沉降速度是一个关键参数,它主要受泥沙粒径分布和流体运动影响。

关键词：振荡网格湍流初始泥沙运动沉积物再悬浮

Incipient sediment motion based on turbulent fluctuations

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International Journal of Sediment Research 2020年第2期35卷 125-133页

作者： wan Hanna Melini wan mohtar Ji wang Lee Najwa Izzaty Mohammad Azha Nian-Sheng Cheng Department of Civil & Structural Engineering Faculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia School of Civil and Environmental Engineering College of EngineeringNanyang Technical University

The current study modifies the representation of the Shields parameter using turbulent strength,*** root-mean-square(rms) fluid *** were done under a steady,uniform flow using eight sediment sizes with particle Reynolds numbers(Rep) ranging between 1.0(fine sediment) and 183.4(coarse sediment).Utilising the peak rms horizontal(u) values,the critical Shields parameter,θ,was calculated and a trend similar to the well-established Shields curve was *** analysis was extended to the Shields curve obtained based on the critical shear velocity,Reynolds shear stress,and data extracted fro m the oscillating grid-turbulence *** show that turbulent fluctuations are crucial for the incipient sediment motion and are essentially better predictors than the commonly used critical shear velocity.A quadrant analysis to identify the role of turbulent bursting events in incipient sediment motion also was done where sweeps and ejections are dominant for finer and coarser sediment sizes,respectively.

关键词： Incipient sediment motion Root-mean-square fluid velocity Grid-turbulence Quadrant analysis Turbulent fluctuations

Roles of fermented plant-,dairy-and meat-based foods in the modulation of allergic responses

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Food Science and Human Wellness 2023年第3期12卷 691-701页

作者： Muhamad Hafi z Abd Rahim Nur Hazlin Hazrin-Chong Hanis Hazeera Harith wan Abd Al Qadr Imad wan-mohtar Rashidah Sukor Faculty of Food Science and Technology Universiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Biological Sciences and Biotechnology Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia43600 BangiSelangorMalaysia Faculty of Medicine and Health Sciences Universiti Putra Malaysia43400 SerdangSelangorMalaysia Functional Omics and Bioprocess Development Laboratory Institute of Biological SciencesFaculty of ScienceUniversiti Malaya50603 Kuala LumpurMalaysia Laboratory of Food Safety and Food Integrity Institute of Tropical Agriculture and Food SecurityUniversiti Putra Malaysia43400 SerdangSelangorMalaysia

This review attempts to delineate the effects and roles of fermented foods on allergic responses(AR),specifically from milk,plant,and meat *** for AR alleviation and aggravation were noted for many different fermented food *** outcomes on AR through fermented foods could be linked to microbial hydrolysis of food allergens,improvement in gut microbiota robustness,and modulation of the immune system that promotes a balance between T helper 1(Th1)and Th2 *** on plant-based,non-protein rich fermented foods tend to show more favourable results compared to those on meat-based or protein-rich *** usage of specific and known starter cultures are helpful in alleviating AR,as in the case for many yogurt,Kefir or Dahi *** fermentation time was also deemed important,exemplified in studies that showed inefficient AR reduction through consumption of fresh ***,formation of new allergens through fermentation of certain meat-based foods,or by using specific fermenting microbes(*** sp.),is ***,combination of starter cultures and food substrates must be considered in preventing or eliminating AR from intake of these *** review may aid consumers to make informed decision during the consumption of fermented food.

关键词： Allergy Fermented food Milk-based allergens Plant-based allergens Animal-based allergens