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DNA-PKcs subunits in radiosensitization by hyperthermia on hepatocellular carcinoma hepG_2 cell line

World journal of Gastroenterology 2002年第5期8卷 797-803页

作者： walter j.curran George Iliakis Department of Radiation Oncology of Kimmel Cancer Center Thomas Jefferson UniversityPhiladelphiaPA 19107USA

AIM: To investigate the role of DNA-PKcs subunits inradiosensitization by hyperthermia on hepatocellularcarcinoma HepG2 cell ***: Hep G2 cells were exposed to hyperthermiaand irradiation. Hyperthermia was given at 45.5 ℃Cellsurvival was determined by an in vitro clonogenic assay forthe cells treated with or without hyperthermia at varioustime points. DNA DSB rejoining was measured usingasymmetric field inversion gel electrophoresis (AFIGE). TheDNA-PKcs activities were measured using DNA-PKcs enzymeassay ***: Hyperthermia can significantly enhanceirradiation-killing cells. Thermal enhancement ratio ascalculated at 10 % survival was 2.02. The difference inradiosensitivity between two treatment modes manifestedas a difference in the α components and the almost sameβ components, which α value was considerably higher inthe cells of combined radiation and hyperthermia ascompared with irradiating cells (1.07 Gy-1 versus 0.44 Gy1). Survival fraction showed 1 logarithm increase after an8-hour interval between heat and irradiation, whereas DNA-PKcs activity did not show any recovery. The cells wereexposed to heat 5 minutes only, DNA-PKcs activity wasinhibited at the nadir, even though the exposure time waslengthened. Whereas the ability of DNA DSB rejoining wasinhibited with the increase of the length of hyperthermictime. The repair kinetics of DNA DSB rejoining aftertreatment with Wortmannin is different from thehyperthermic group due to the striking high slow ***: Determination with the cell extracts andthe peptide phosphorylation assay, DNA-PKcs activity wasinactivated by heat treatment at 45.5 C, and could notrestore. Cell survival is not associated with the DNA-PKcsinactivity after heat. DNA-PKcs is not a unique factor affectingthe DNA DSB repair. This suggests that DNA-PKcs do notplay a crucial role in the enhancement of cellularradiosensitivity by hyperthermia.

关键词：肝癌放射治疗放射敏感性催化作用 DNA激酶

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The role of DNA-PK subunits in radiosensitization by hyperthermia on hepatoceUular carcinoma HepG2 cell line

The role of DNA-PK subunits in radiosensitization by hyperth...

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2000全国肿瘤学术大会

作者： walter j.curran George Iliakis Department of Radiation Oncology of Kimmel Cancer Center Thomas Jefferson UniversityPhiladelphiaPa 19107 Jr. Department of Radiation Oncology of Kimmel Cancer Center Thomas Jefferson UniversityPhiladelphiaPa 19107

＜正＞Purpose:Thermoradiotherapy currently offers the most significant advantages in the treatment of primary liver cancer and other types of cancer. The cause of this radiosensitization has not been firmly established,in part this sensitization is thought to be through inhibition of repair of radiation induced DNA ***-dependent protein kinase（DNA-PK）plays a central role in the repair of DNA double-strand break（dsb）.We investigated the role of DNA-PK subunits in rediosensitization by hyperthermia on hepatocel- lular carcinoma HepG2 cell *** and Materials:Hep G2 cells were exposed to hyperthermia and *** was given at 45.5(C. Cell survival was determined by an in vitro clonogenic assay for the cells treat- ed with irradiated with or without hyperthermia at various times.

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The Role of DNA-PKcs Subunits in Radiosensitization by Hyperthermia:A Query into the Current Determination of DNA-PKcs

The Role of DNA-PKcs Subunits in Radiosensitization by Hyper...

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2000全国肿瘤学术大会

作者： walter j.curran George Iliakis jr.M.D.Department of Radiation Oncology of Kimmel Cancer Center Thomas Jefferson UniversityPhiladelphiaPa 19107 Department of Radiation Oncology of Kimmel Cancer Center Thomas Jefferson UniversityPhiladelphiaPa 19107

＜正＞Objects:Double-strand breaks（DSBs）can be efficiently re- moved from the DNA of higher eukaryotes by nonhomologous end-joining （NHEj）.The exact mechanisn and the regulation of NHEj remain to be elu- cidated,genetic and biochemical evidence implicate DNA-dependent protein kinase（DNA-PKcs）in the process.A possible mechanism for thermo-ra- diosensitization is mediated through the heat lability of Ku subunits of DNA- PKcs,however,the contradictory results showed that DNA-PKcs is heat re- *** understand the role of DNA-PKcs in radiosensitization by hyper- thermia,we compared rejoining of DNA DSBs and survival fraction in the cells of DNA-PKcs-proficiency and-deficiency in the combination with or without hyperthermia.

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三种回收昆虫病原线虫方法其回收效率的比较研究初报

三种回收昆虫病原线虫方法其回收效率的比较研究初报

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全国生物防治学术讨论会

作者：简恒杨怀文 j.curran 中国农科院生防所 CSIRO Division of Entomology Canberra 2601

利用昆虫病原线虫防治农、林、果树害虫是近年来发展起来的一种新的生物防治手段,具有广泛的应用前景。采用喷施、浇注线虫悬浮液于害虫生活环境,通过线虫主动寻找、侵染寄主达到防虫目的。为准确评价经释放后的线虫在田间的存活情况,...

利用昆虫病原线虫防治农、林、果树害虫是近年来发展起来的一种新的生物防治手段,具有广泛的应用前景。采用喷施、浇注线虫悬浮液于害虫生活环境,通过线虫主动寻找、侵染寄主达到防虫目的。为准确评价经释放后的线虫在田间的存活情况,对害虫

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Intraspecific variation in shoot flammability in Dracophyllum rosmarinifolium is not predicted by habitat environmental conditions

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Forest Ecosystems 2022年第2期9卷 178-185页

作者： Xinglei Cui Adrian M.Paterson George LW.Perry Sarah V.Wyse Md Azharul Alam Congde Huang Shixing Zhou Lin Xiao Changhong Lai Fang He Dongyu Cao Kate Marshall Timothy j.curran Department of Pest-management and Conservation Lincoln UniversityLincoln7647New Zealand National Forestry and Grassland Administration Engineering Research Centre for Southwest Forest and Grassland Fire Ecological Prevention College of ForestrySichuan Agricultural UniversityChengdu611130China School of Environment University of AucklandAuckland1142New Zealand Bio-Protection Research Centre Lincoln UniversityLincoln7647New Zealand Sichuan Province Forestry and Grassland Environmental Monitoring Centre Chengdu610036China

Background:Flammability is a compound plant trait that can vary significantly across natural populations within *** intraspecific variation in flammability provides insights into the evolution of plant flam-mability and inform understanding of wildfire risk and behaviour in different ***:We measured four flammability variables,representing ignitibility(time to ignition),sustainability(total burning time),combustibility(maximum temperature during burning)and consumability(percentage of biomass consumed by fire)to assess the shoot-level flammability of Dracophyllum rosmarinifolium(***.)(Ericaceae),a polymorphic endemic species distributed throughout New *** examined the relationship between flammability components and a suite of climatic and geographic variables(elevation,latitude,mean annual temperature(MAT),mean annual rainfall(MAR)of the sample locations,etc.).Results:We measured shoot-level flammability components of 62 individuals across eight *** time,maximum temperature and burnt biomass were positively correlated with each other,while ignition score was independent of other flammability *** flammability components varied significantly across the eight *** habitat conditions we considered were not related to any of the shoot-level flammability components of ***:Intraspecific variation in flammability in *** may be a byproduct of selection on other functional traits,such as leaf size,shoot lipid content,indicating that plant flammability is an incidental result,rather than selected for,at least in ecosystems without fire as a selective force.

关键词： Dracophyllum Fire Flammability Habitat

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光敏电路在控制汞电极形成过程的应用

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厦门大学学报（自然科学版） 1993年第3期32卷 332-335页

作者：邓志威 curran D j 美国麻省州立大学化学系

在一个新设计的以汞为电极流动注入分析体系中,蠕动泵不能提供恒定流速,因而常用的时钟法已很难准确地控制体系,为此,采用了光敏耦台器来代替时钟控制整个分析体系,与时钟法比较,流动体系的流速对光敏电路的影响不大,因而系统控制过程... 详细信息

在一个新设计的以汞为电极流动注入分析体系中,蠕动泵不能提供恒定流速,因而常用的时钟法已很难准确地控制体系,为此,采用了光敏耦台器来代替时钟控制整个分析体系,与时钟法比较,流动体系的流速对光敏电路的影响不大,因而系统控制过程更接近该体系的设计要求。

关键词：光敏耦合器汞电极光敏电路

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应用染色体组DNA限制性片段长度差异鉴定线虫种

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兽医导刊 1987年第2期 62-64页

作者： j.curran 秦泽云内蒙古农牧学院兽医系

材料和方法一、染色体组DNA的提取采集湿重约100mg线虫,双蒸水冲洗3次,在微型离心机中离心形成小团块。将线虫小团块加于1.5ml微型离心管中,于浓度为2 mg/ml蛋白酶K液[0.1M三羟甲基氨基甲烷(Tris)PH8.5 0.05M乙二胺四乙酸(EDTA)1.2MNaCl... 详细信息

材料和方法一、染色体组DNA的提取采集湿重约100mg线虫,双蒸水冲洗3次,在微型离心机中离心形成小团块。将线虫小团块加于1.5ml微型离心管中,于浓度为2 mg/ml蛋白酶K液[0.1M三羟甲基氨基甲烷(Tris)PH8.5 0.05M乙二胺四乙酸(EDTA)1.2MNaCl和1%十二烷基硫酸钠]

关键词：限制性片段长度虫体 DNA 差异线虫蠕虫染色体组基因组

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Identifying contextual determinants of problems in tuberculosis care provision in South Africa: a theory-generating case study

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Infectious Diseases of Poverty 2021年第3期10卷 82-94页

作者： jamie Murdoch Robyn curran Andre jvan Rensburg Ajibola Awotiwon Audry Dube Max Bachmann Inge Petersen Lara Fairall School of Health Sciences University of East AngliaNorwich NR47TJUK. University of Cape Town Lung Institute Knowledge Translation UnitUniversity of Cape TownMowbray 7700South Africa Centre for Rural Health University of KwaZulu NatalDurbanSouth Africa Norwich Medical School University of East AngliaNorwich NR47TJUK. King's Global Health Institute King's College LondonLondon SE19NHUK. Knowledge Translation Unit Department of MedicineUniversity of Cape Town Lung InstituteUniversity of Cape TownMowbray 7700South Africa.

Background:Despite progress towards End TB Strategy targets for reducing tuberculosis(TB)incidence and deaths by 2035,South Africa remains among the top ten high-burden tuberculosis countries globally.A large challenge lies in how policies to improve detection,diagnosis and treatment completion interact with social and structural drivers *** understanding and theoretical development of the contextual determinants of problems inTB care is required for developing effective *** article reports findings from the pre-implementation phase of a study ofTB care in South Africa,contributing to HeAlth System StrEngThening in Sub-Saharan Africa(ASSET)-a five-year research programme developing and evaluating health system strengthening interventions in sub-Saharan *** study aimed to develop hypothetical propositions regarding contextual determinants of problems inTB care to inform intervention development to reduce TB deaths and incidence whilst ensuring the delivery of quality integrated,person-centred ***:Theory-building case study design using the Context and Implementation of Complex Interventions(CICI)framework to identify contextual determinants of problems in TB *** February and November 2019,we used mixed methods in six public-sector primary healthcare facilities and one public-sector hospital serving impoverished urban and rural communities in the Amajuba District of KwaZulu-Natal Province,South *** data included stakeholder interviews,observations and documentary *** data included routine data on sputum testing andTB *** were inductively analysed and mapped onto the seven CICI contextual ***:Delayed diagnosis was caused by interactions between fragmented healthcare provision;limited resources;verticalised care;poorTB screening,sputum collection and *** nurse responsible forTB care,with limited integration ofTB with other conditions,and policy focused o

关键词： Tuberculosis Health systems strengthening Primary healthcare Person-centred care Context

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纪录片也要讲故事

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作者： (美)希拉·柯伦·伯纳德(Sheila curran Bernard)

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读者来信：Brava非手术乳房增大系统的初期应用经验

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中国美容整形外科杂志 2012年第12期23卷 I0029-I0032页

作者： curran j. Smith 魏峰(译) 高景恒(译) 张晨(译) 美国华盛顿特区西雅图华盛顿大学医学院《中国美容整形外科杂志》编辑部大连大学附属新华医院

在过去的6个月中,我应用Brava乳房增大系统（BRAVA公司,美国佛罗里达州椰林）进行非手术隆乳术.我的初期应用结果与Khouri等报道的不一致,同时,我认为有必要与将要应用该产品的外科医师分享我的信息.我们机构中的许多资深成员都对Brava... 详细信息

在过去的6个月中,我应用Brava乳房增大系统（BRAVA公司,美国佛罗里达州椰林）进行非手术隆乳术.我的初期应用结果与Khouri等报道的不一致,同时,我认为有必要与将要应用该产品的外科医师分享我的信息.我们机构中的许多资深成员都对Brava这一产品表示认可,而且乳房非常小的患者施行隆乳术又因乳房假体的尺寸受限也一直是困扰我的问题,所以,当读到杂志报道的Khouri等应用Brava所取得的良好效果,我就被吸引住了.

关键词：乳房增大非手术应用读者来信系统佛罗里达州外科医师乳房假体

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