The Role of DNA-PKcs Subunits in Radiosensitization by Hyperthermia:A Query into the Current Determination of DNA-PKcs
作者单位：Jr.M.D.Department of Radiation Oncology of Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphiaPa 19107 Department of Radiation Oncology of Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphiaPa 19107
会议名称：《2000全国肿瘤学术大会》
会议日期：2000年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
摘 要：正Objects:Double-strand breaks（DSBs）can be efficiently re- moved from the DNA of higher eukaryotes by nonhomologous end-joining （NHEJ）.The exact mechanisn and the regulation of NHEJ remain to be elu- cidated,genetic and biochemical evidence implicate DNA-dependent protein kinase（DNA-PKcs）in the process.A possible mechanism for thermo-ra- diosensitization is mediated through the heat lability of Ku subunits of DNA- PKcs,however,the contradictory results showed that DNA-PKcs is heat re- *** understand the role of DNA-PKcs in radiosensitization by hyper- thermia,we compared rejoining of DNA DSBs and survival fraction in the cells of DNA-PKcs-proficiency and-deficiency in the combination with or without hyperthermia.