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A secretory function of human insulin-producing cells in vivo

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Hepatobiliary & Pancreatic Diseases International 2009年第3期8卷 255-260页

作者： Hu, Yan-Hua wu, de-quan Gao, Feng Li, Guo-Dong Yao, Lei Zhang, Xin-Chen Harbin Med Coll Dept Gen Surg Affiliated Hosp 2 Harbin 150086 Peoples R China

BACKGROUND: Mesenchymal stem cells derived from human umbilical cord blood (UCB-MSCs) have good research and application prospects in the treatment of diabetes. We once induced UCB-MSCs to differentiate into insulin-producing cells (IPCs) in vitro, but we did not know the functions of these cells in vivo. The aim of this study was to assess the functional effects of IPCs on insulin secretion and their role in the treatment of diabetes in vivo. METHODS: UCB-MSCs were induced to IPCs by an inducing protocol with extracellular matrix gel. BALB/C nude mice were made hyperglycemic by intraperitoneal injection of streptozotocin. The diabetic mice were transplanted with 1x10(7) IPCs under the renal capsule or with phosphate-buffered saline as a control. After transplantation, the grafts were analyzed by immunocytochemistry for the expression of human insulin; the serum human insulin levels were measured; and blood glucose and body weight status were monitored. RESULTS: Immunofluorescence showed that numerous IPCs under the kidney capsule were insulin-positive. On day 14 after transplantation, the serum human insulin level of the treatment group (n=9) averaged 0.44 +/- 0.12 mU/L, which was higher than that of the control group (n=9) that did not express insulin (t=10.842, Pded to make further use of them to regulate hyperglycemia and body weight in vivo. (Hepatobiliary Pancreat Dis Int 2009; 8: 255-260)

关键词： human umbilical cord blood mesenchymal stem cells insulin-producing cells diabetes cell transplantation

Extracellular matrix gel is necessary for in vitro cultivation of insulin producing cells from human umbilical cord blood derived mesenchymal stem cells

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Chinese Medical Journal 2008年第9期121卷 811-818页

作者： GAO Feng wu de-quan HU Yan-hua JIN Guang-xin department of General Surgery the Second Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150086 China

Background Pancreatic islet cell transplantation is an effective approach to treat type 1 diabetes. However, this therapy is not widely used because of the severe shortage of transplantable donor islets. This study investigated whether mesenchymal stem cells （MSCs） derived from human umbilical cord blood （UCB） could be transdifferentiated into insulin producing cells in vitro and the role of extracellular matrix （ECM） gel in this procedure. Methods Human UCB samples were collected and MSCs were isolated. MSCs specific marker proteins were analyzed by a flow cytometer, The capacities of osteoblast and adipocyte to differentiate were tested. Differentiation into islet like cell was induced by a 15-day protocol with or without ECM gel. Pancreatic characteristics were evaluated with immunofluorescence, reverse transcription polymerase chain reaction （RT-PCR） and flow cytometry. Insulin content and release in response to glucose stimulation were detected with chemiluminescent immunoassay system. Results Sixteen MSCs were isolated from 42 term human UCB units （38%）. Human UCB-MSCs expressed MSCs specific markers and could be induced in vitro into osteoblast and adipocyte. Islet like cell clusters appeared about 9 days after pancreatic differentiation in the inducing system with ECM gel. The insulin positive cells accounted for （25.2~3.4）% of the induced cells. The induced cells expressed islet related genes and hormones, but were not very responsive to glucose challenge. When MSCs were induced without ECM gel, clusters formation and secretion of functional islet proteins could not be observed, Conclusions Human UCB-MSCs can differentiate into islet like cells in vitro and ECM gel plays an important role in pancreatic endocrine cell maturation and formation of three dimensional structures.

关键词： umbilical cord blood mesenchymal stem cells induction insulin producing cells extracellular matrix

Notch signaling： a novel regulating differentiation mechanism of human umbilical cord blood-derived mesenchymal stem cells into insulin-producing cells in vitro

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Chinese Medical Journal 2010年第5期123卷 606-614页

作者： HU Yan-hua wu de-quan GAO Feng LI Guo-dong ZHANG Xin-chen department of General Surgery Second Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150086 China

Background Human umbilical cord blood-derived mesenchymal stem cells （UCB-MSCs） could be induced to differentiate into insulin producing cells （IPCs） in vitro, which have good application potential in the cell replacement treatment of type-1 diabetes. However, the mechanisms regulating this differentiation have remained largely unknown. Notch signaling is critical in cell differentiation. This study investigated whether Notch signaling could regulate the IPCs differentiation of human UCB-MSCs. Methods Using an interfering Notch signaling protocol in vitro, we studied the role of Notch signaling in differentiation of human UCB-MSCs into IPCs. In a control group the induction took place without interfering Notch signaling. Results Human UCB-MSCs expressed the genes of Notch receptors （Notch 1 and Notch 2） and ligands （Jagged 1 and deltalike 1）. Human UCB-MSCs with over-expressing Notch signaling in differentiation resulted in the down-regulation of insulin gene level, proinsulin protein expression, and insulin-positive cells percentage compared with the control group. These results showed that over-expressing Notch signaling inhibited IPCs differentiation. Conversely, when Notch signaling was attenuated by receptor inhibitor, the induced cells increased on average by 3.06-fold （n=-4, P 〈0.001） in insulin gene level, 2.60-fold （n=-3, P 〈0.02） in proinsulin protein expression, and 1.62-fold （n=-6, P 〈0.001） in the rate of IPCs compared with the control group. Notch signaling inhibition significantly promoted IPCs differentiation with about 40% of human UCB-MSCs that converted to IPCs, but these IPCs were not responsive to glucose challenge very well both in vitro and in vivo. Hence, further research has to be carried out in the future. Conclusions Notch signaling may be an important mechanism regulating IPCs differentiation of human LICB-MSCs in vitro and Notch signaling inhibition may be an efficient way to increase the number of IPCs, which may resolve the shortage of

关键词： Notch signaling mesenchymal stem cells differentiation insulin producing cells diabetes

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复发性腹膜后脂肪肉瘤诊治分析

中国实用外科杂志 2014年第4期34卷 336-338页

作者：吴德全哈尔滨医科大学附属第二医院普外科黑龙江哈尔滨150086

目的探讨复发性腹膜后脂肪肉瘤的临床特点、复发因素及诊治方法。方法回顾性分析哈尔滨医科大学附属第二医院2002—2012年收治的37例腹膜后脂肪肉瘤病人13例22次复发的临床资料。结果复发病例较初发者更容易引发临床症状和侵袭周围组织... 详细信息

目的探讨复发性腹膜后脂肪肉瘤的临床特点、复发因素及诊治方法。方法回顾性分析哈尔滨医科大学附属第二医院2002—2012年收治的37例腹膜后脂肪肉瘤病人13例22次复发的临床资料。结果复发病例较初发者更容易引发临床症状和侵袭周围组织或脏器,手术完全切除率低;随着复发次数的增加,复发间期逐渐缩短,恶性度增加,生存期明显缩短。结论腹膜后间隙的特殊性和肿瘤形态结构的多样性是导致腹膜后脂肪肉瘤不易被彻底切除和术后复发的重要因素,强调首次完全完整和彻底的切除是防止复发、改善预后的关键。

关键词：腹膜后脂肪肉瘤复发临床特征无瘤术

根因分析法在急诊急救护理不良事件管理中应用的效果评价

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中国实用护理杂志 2013年第28期29卷 72-74页

作者：吴德全安徽医科大学第二附属医院护理部合肥230601

目的减少急诊急救护理不良事件的发生，提高护理安全。方法成立质量控制（qualitycontrol，QC）小组，运用根本原因分析法（rootcauseanalysis，RCA）分析2008年12月至2011年12月急诊急救中发生的护理不良事件近端原因与根本原因，并... 详细信息

目的减少急诊急救护理不良事件的发生，提高护理安全。方法成立质量控制（qualitycontrol，QC）小组，运用根本原因分析法（rootcauseanalysis，RCA）分析2008年12月至2011年12月急诊急救中发生的护理不良事件近端原因与根本原因，并对根本原因提出整改措施。通过加强规章制度执行规范督查，改变护理排班模式，护士风险意识的强化教育以及加强护士的沟通技巧培训，重视日常护理工作中的细节管理等根本原因的改进。结果2012年12月进行追踪分析，急诊急救护理不良事件的发生由2011年占全院护理不良事件的9．81％下降为2012年的2．75％。结论完善相关流程与制度督查，加强护士急诊急救能力与护理危机意识培养，实行弹性排班，重视细节管理是减少急诊急救护理不良事件发生的关键所在。

关键词：安全管理护理不良事件根本原因分析急诊急救

Splenic hamartoma: case report and review of literature

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Chinese Medical Journal 2006年第16期119卷 1403-1408页

作者： JIA Hong-bo LI Ying-ping HAN de-en LIU Yao ZHANG Bin wu de-quan CHEN Xi JIANG Ying ZHENG Long-xian DU Jin-rong JIANG Xue-hai department of Surgery Second Affiliated Hospital of HarbinMedical University Harbin 150086 China Extracorporeal Shock Wave Lithotripsy Center Second AffiliatedHospital of Harbin Medical University Harbin 150086 China department of Pathology Second Affiliated Hospital of HarbinMedical University Harbin 150086 China

Splenic hamartoma is a benign malformation composed of an anomalous mixture of normal splenic elements.1 Approximately half of the patients with the tumor are asymptomatic and diagnosed accidentally by autopsy or splenectomy. The tumor-related renal hematological and dermatological abnormalities can be treated by removal of the tumor. Splenic hamartoma which is related to renal diseases including extramembranous glomerulonephritis, membranoproliferative glomerulonephritis and renal adenocarcinoma has been reported previously.

关键词： hamartoma splenic neoplasm kidney calculi splenectomy

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao de-En Xu Ming-Lei wu Ji-Chuan Liu Zi-Ling Shi quan-Hong Ma department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

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急诊室创伤复苏研究进展

中华创伤杂志 2011年第2期27卷 189-192页

作者：高峰吴德全哈尔滨医科大学附属第二医院肝脏外科 150086

根据实施的时间与地点，对创伤患者的急救与复苏可分为以下三个阶段：院前复苏、急诊室复苏、后期复苏。近年来，创伤复苏的策略与观念取得了较大发展。

关键词：院前复苏创伤患者急诊室

稀土对金属纳米粒子-介质复合薄膜(Ag-BaO)光电发射性能的增强

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物理学报 2001年第5期50卷 977-980页

作者：许北雪吴锦雷刘惟敏杨海邵庆益刘盛薛增泉吴全德北京大学电子学系北京100871

用真空蒸发沉积的方法制备了掺杂稀土的金属纳米粒子介质复合薄膜 (Ag BaO薄膜 ) .与不掺杂稀土的Ag BaO薄膜相比 ,其光电发射能力提高了近 40 % .透射电镜分析表明 ,掺杂稀土后 ,Ag BaO薄膜中的Ag纳米粒子明显细化、球化、密度增大 ... 详细信息

用真空蒸发沉积的方法制备了掺杂稀土的金属纳米粒子介质复合薄膜 (Ag BaO薄膜 ) .与不掺杂稀土的Ag BaO薄膜相比 ,其光电发射能力提高了近 40 % .透射电镜分析表明 ,掺杂稀土后 ,Ag BaO薄膜中的Ag纳米粒子明显细化、球化、密度增大 .这表明Ag纳米粒子的细化 ,使得其在光作用下 ,光电子更容易通过隧道效应穿过界面位垒逸出。

关键词：光电发射稀土纳米粒子真空蒸发沉积 Ag-BaO薄膜