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Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Journal of Oceanology and Limnology 2019年第1期37卷 210-222页

作者： YIN Qunjian ZHANG Weijia LI Xuegong ZHOU Lihong qi Xiaoqing ZHANG Chan wu Long-Fei Laboratory of Deep-sea Microbial Cell Biology Institute of Deep-sea Science and Engineering Chinese Academy of Sciences University of Chinese Academy of Sciences International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms CNRS-Marseille/CAS-Beijing-Qingdao-Sanya CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions Institute of Deep-sea Science and Engineering Chinese Academy of Sciences AMU LCB UMR 7283 CNRS-Marseille

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.

关键词： marine bacteria Trimethylamine N-oxide(TMAO) high hydrostatic pressure(HHP) pressure tolerance phenotype

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黄河三角洲中深层地下淡水资源可持续保护探讨

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环境生态学 2024年第4期6卷 39-42,49页

作者：逯春民吴晓霞隋海波东营市生态环境局山东东营257300 南京晓庄学院环境科学学院江苏南京211171 山东省地矿工程勘察院山东济南250014

21世纪以来,黄河三角洲地区工农业经济快速发展,造成南部区域中深层地下水超量开采,对地下淡水资源的可持续利用构成较大压力。基于当前黄河流域生态保护和高质量发展的需求,为保障地下淡水的环境安全,结合该区域中深层地下水环境现状,... 详细信息

21世纪以来,黄河三角洲地区工农业经济快速发展,造成南部区域中深层地下水超量开采,对地下淡水资源的可持续利用构成较大压力。基于当前黄河流域生态保护和高质量发展的需求,为保障地下淡水的环境安全,结合该区域中深层地下水环境现状,提出了控制中深层地下淡水合理开采、优化水资源配置等地下淡水开发利用保护的建议。

关键词：地下淡水可持续保护黄河三角洲

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江西南昌及浙江衢州发现东亚水鼠耳蝠

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动物学杂志 2024年第1期59卷 121-128页

作者：邓瑾何向阳郭敏罗鹏飞吴诗宝张礼标华南师范大学生命科学学院广州510631 广东省科学院动物研究所广东省动物保护与资源利用重点实验室广州510260

2020年5至9月,在江西省南昌市向塘机场七里港火车桥底、高访立交桥底、横岗铁路桥底和浙江省衢州市衢州机场西山村涵洞分别捕获到1号(雌性)、4号(2雄性、2雌性)、2号(2雄性)和1号(雌性)鼠耳蝠。上述鼠耳蝠体型较小,头体长42.89~51.41 mm... 详细信息

2020年5至9月,在江西省南昌市向塘机场七里港火车桥底、高访立交桥底、横岗铁路桥底和浙江省衢州市衢州机场西山村涵洞分别捕获到1号(雌性)、4号(2雄性、2雌性)、2号(2雄性)和1号(雌性)鼠耳蝠。上述鼠耳蝠体型较小,头体长42.89~51.41 mm,前臂长40.18~42.22 mm;头骨较小,脑颅略低,顶部平缓,矢状脊不明显,吻长较短,吻宽较长,超过眶间宽,颧弓纤细且发达,宽度明显超过后头宽,明显区别于该地区已报道的鼠耳蝠物种。通过外部形态鉴定以及Cyt b、COI序列比对和系统发育关系结果,鉴定上述标本为东亚水鼠耳蝠(Myotis petax),且该种是在江西省和浙江省翼手目分布新记录种。上述标本保存于广东省科学院动物研究所。

关键词：东亚水鼠耳蝠翼手目江苏省浙江省分布新记录

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Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats

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Journal of Integrative Agriculture 2013年第2期12卷 319-326页

作者： LIU Da-cheng ZHOU Xiang-li ZHAO Pei-ting GAO min HAN Hao-qi HU Hong-lian College of Veterinary Medicine Inner Mongolia Agricultural University Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of ministry of Agriculture Animal Nutrition Institute of Agriculture and Animal Husbandry Academy of Inner Mongolia

Six rumen-cannulated lactating Guanzhong goats were used to investigate changes in ruminal fermentation pattern and the microbiota following a subacute rtmainal acidosis （SARA） inducing procedure. Induction of SARA was performed by increasing dietary non-fiber carbohydrate （NFC） to neutral detergent fiber （NDF） ratio from 1.02 （stage 1） to 1.24 （stage 2）, 1.63 （stage 3） and 2.58 （stage 4）. A dynamic pH monitoring system, real-time fluorescent quantitative PCR and conventional anaerobic culture were used to assess changes in ruminal pH and microbiota. Results indicated that rumen fermentation patterns changed significantly with increased NFC：NDF ratio. The decline in ruminal pH was caused by increased ruminal total volatile fatty acids （TVFA）, which was mainly attributed to a significant increase in ruminal butyrate, rather than the accumulation of ruminal lactic acid. In addition, in the course of SARA, the number of rumen microoganisms altered significantly, with increases in ruminal amylolytic bacteria, Lactobacilli, Streptococcus bovis and Megasphaera elsdenii, the latter particularly dramatically indicating that it may be the main factor responsible for the increase in butyrate, and decrease in protozoa.

关键词： rumen fermentation rumen microorganisms subacute ruminal acidosis goat

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罐式煅烧炉产能因素分析及应用

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云南冶金 2024年第1期53卷 168-172页

作者：段斌李春民李自田吴建茂云南源鑫炭素有限公司云南建水654300

针对罐式煅烧炉生产运行中出现的首二层温度提升难、频繁“放炮”、料罐硅砖使用寿命短、达不到设计产能等问题,采取了预热空气通道优化、含尘废气收集、石油焦精准配料等一系列改造措施,并规范了罐式炉日常操作方式,使首二层温度提升5... 详细信息

针对罐式煅烧炉生产运行中出现的首二层温度提升难、频繁“放炮”、料罐硅砖使用寿命短、达不到设计产能等问题,采取了预热空气通道优化、含尘废气收集、石油焦精准配料等一系列改造措施,并规范了罐式炉日常操作方式,使首二层温度提升50℃以上,“放炮”现象基本消除,单罐产能突破设计值100 kg/h。

关键词：煅烧炉产量温度放炮硫含量硅砖使用寿命

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Process study of atomic layer deposition Al2O3in industryapplication for solar cells

Process study of atomic layer deposition Al2O3in industryapp...

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第八届中国功能材料及其应用学术会议

作者： qiU Hong-bo LI Hui-qi LIU Bang-wu XIA Yang School of Materials Science and Engineering Shandong university of science and technology Key Laboratory of Microelectronics Devices & Integrated Technology Instituteof MicroelectronicsChinese Academy of Sciences

ISBN: (纸本)9783037859698

The rear surface of multi-silicon has been passivated by Atomic Layer Deposition(ALD) AlOand Plasma Enhanced Chemical Vapor Deposition(PECVD) *** results of the effective lifetime of the silicon before and after firing show that the thickness of AlO thinfilms has a significant effect on the passivation *** effective lifetime of the silicon can reach up to 40.64μs and show a better passvation quality when the thickness of AlO film is18 ***,the impact of spacing of the laser opening-line contact on open-circuit voltage and fillfactor has been furthermore *** cells have a better performance when the spacing of the opening-line is *** result of the local topography of the opening-line contact tested by Scanning Electron Microscopy(SEM) shows that the existing of the void in the interface between Al and Si is the main reason for the bad performance of the cells.

关键词： Atomic Layer Deposition Al2O3,Multi-silicon minority carrier lifetime Laser Opening-line contact

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人工智能在胸部骨折CT诊断中的应用

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广东医学 2024年第8期45卷 993-997页

作者：刘玉蒙吴若岱陆超吕云罡叶海陈靓周敏敏李光耀吴松雄吴光耀深圳大学总医院放射科广东深圳518055

目的探讨人工智能骨疾病诊断系统(AI)在胸部骨折中的诊断效能及应用价值。方法回顾性分析深圳大学总医院因创伤急诊就诊行胸部CT并经3~6周复查胸部CT证实的726例胸部骨折,计算AI和两名放射科医师以及放射科医师在AI辅助下对胸部骨折诊... 详细信息

目的探讨人工智能骨疾病诊断系统(AI)在胸部骨折中的诊断效能及应用价值。方法回顾性分析深圳大学总医院因创伤急诊就诊行胸部CT并经3~6周复查胸部CT证实的726例胸部骨折,计算AI和两名放射科医师以及放射科医师在AI辅助下对胸部骨折诊断的召回率、精确率和F1分数。结果AI检测肋骨骨折的召回率和F1分数分别为0.91和0.92,均高于放射科医师1(0.77与0.85)和放射科医师2(0.84与0.90),AI的精确率(0.92)低于放射科医师1(0.95)和放射科医师2(0.96)。放射科医师1和2在AI辅助下肋骨骨折检测的召回率、精确率和F1分数分别为0.94、0.95、0.94和0.97、0.98、0.97。AI检测胸部其他骨折的召回率和F1分数(0.90与0.90)高于放射科医师1(0.62与0.74)和放射科医师2(0.73与0.81),放射科医师1和2在AI辅助下,胸部其他骨折检测的召回率、精确率和F1分数分别为0.94、0.95、0.94和0.97、0.97、0.97。结论AI能高效高敏感地检测急诊创伤患者胸部CT中的胸部骨折,有望优化急诊创伤患者的诊疗流程。

关键词：体层摄影术胸部深度学习创伤急诊人工智能

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A "turn-off" luminescence resonance energy transfer aptamer sensor based on near-infrared upconverting NaYF_4:Yb^(3+),Tm^(3+) nanoparticles as donors and gold nanorods as acceptors

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Chinese Chemical Letters 2013年第1期24卷 79-81页

作者： Hong-qi Chen Juan Xu Fei Yuan Yong wu Yi-Yan Zhang Lun Wang Anhui Key Laboratory of Chemo-Biosensing College of Chemistry and Materials ScienceAnhui Normal University

Near-infrared upconverting NaYF4：Yb3＋, Tm3＋ nanophosphors modified with poly（acrylic acid） were prepared and characterized by transmission electron microscopy and luminescence spectroscopy. Based on the observed overlap between the emission spectrum of the NaYF4：Yb3＋, Tm3＋ nanophosphors and the absorption spectrum of the gold nanorods, we believe that a new “turn-off” luminescence resonance energy transfer aptamer sensor was constructed for sensing thrombin in near-infrared region.

关键词： NaYF4:Yb3+ Tm3+ nanophosphorsGold nanorodsThrombinNear-infrared

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ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and PI4KB to Assist the Assembly of Replication Organelles

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Virologica Sinica 2021年第6期36卷 1387-1399页

作者： qiuhan Zhang Siliang Li Ping Lei Zixian Li Feifei Chen qi Chen Yulu Wang Jiami Gong qi Tang Xinjin Liu Ke Lan Shuwen wu State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan430072China Medical Research Institute Wuhan UniversityWuhan430072China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan430072China

Similar to that of other enteroviruses, the replication of enterovirus 71(EV71) occurs on rearranged membranous structures called replication organelles(ROs). Phosphatidylinositol 4-kinase Ⅲ(PI4KB), which is required by enteroviruses for RO formation, yields phosphatidylinositol-4-phosphate(PI4P) on ROs. PI4P then binds and induces conformational changes in the RNA-dependent RNA polymerase(Rd Rp) to modulate Rd Rp activity. Here, we targeted 3D polymerase, the core enzyme of EV71 ROs, and found that the host factor Annexin A2(ANXA2) can interact with 3 D polymerase and promote the replication of EV71. Then, an experiment showed that the annexin domain of ANXA2, which possesses membranebinding capacity, mediates the interaction of ANXA2 with EV71 3 D polymerase. Further research showed that ANXA2 is localized on ROs and interacts with PI4KB. Overexpression of ANXA2 stimulated the formation of PI4P, and the level of PI4P was decreased in ANXA2-knockout cells. Furthermore, ANXA2, PI4KB, and 3D were shown to be localized to the viral RNA replication site, where they form a higher-order protein complex, and the presence of ANXA2 promoted the PI4 KB-3D interaction. Altogether, our data provide new insight into the role of ANXA2 in facilitating formation of the EV71 RNA replication complex.

关键词： Enterovirus 71(EV71) Annexin A2(ANXA2) 3D polymerase Phosphatidylinositol 4-kinaseⅢ(PI4KB)

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Behavior recognition based on the fusion of 3D-BN-VGG and LSTM network

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High Technology Letters 2020年第4期26卷 372-382页

作者： wu Jin min Yu Shi qianwen Zhang Weihua Zhao Bo School of Electronic and Engineering Xi’an University of Posts and TelecommunicationsXi’an 710121P.R.China

In order to effectively solve the problems of low accuracy,large amount of computation and complex logic of deep learning algorithms in behavior recognition,a kind of behavior recognition based on the fusion of 3 dimensional batch normalization visual geometry group(3D-BN-VGG)and long short-term memory(LSTM)network is *** this network,3D convolutional layer is used to extract the spatial domain features and time domain features of video sequence at the same time,multiple small convolution kernels are stacked to replace large convolution kernels,thus the depth of neural network is deepened and the number of network parameters is *** addition,the latest batch normalization algorithm is added to the 3-dimensional convolutional network to improve the training *** the output of the full connection layer is sent to LSTM network as the feature vectors to extract the sequence *** method,which directly uses the output of the whole base level without passing through the full connection layer,reduces the parameters of the whole fusion network to 15324485,nearly twice as much as those of ***,it reveals that the proposed network achieves 96.5%and 74.9%accuracy in the UCF-101 and HMDB-51 respectively,and the algorithm has a calculation speed of 1066 fps and an acceleration ratio of 1,which has a significant predominance in velocity.

关键词： behavior recognition deep learning 3 dimensional batch normalization visual geometry group(3D-BN-VGG) long short-term memory(LSTM)network

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