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Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea

International Journal of Ophthalmology(English edition) 2016年第3期9卷 325-331页

作者： Xian-Ning Liu Xiu-ping Zhu Jie wu Zheng-Jie wu Yong Yin Xiang-HuaXiao Xin Su Bin Kong Shi-Yin Pan Hua Yang Yan Cheng Na An Sheng-Li Mi Shaanxi Institute of Ophthalmology Xi'an 710002 Shaanxi Province China Shaanxi Key Laboratory of Eye Xi'an 710002 Shaanxi Province China Xi'an First Hospital Xi'an 710002 Shaanxi Province China Biomanufacturing Engineering Laboratory Graduate School at Shenzhen Tsinghua University Shenzhen 518055Guangdong Province China

AIM： To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS： A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy （TEM） and hematoxylin and eosin （H＆E） staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype, RESULTS： The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process, in vitro, the methyl thiazolyl tetrazoUum results revealed that extracts of the acellular ostrich corneas （AOCs） had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes, The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea （APC） transplantation, The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer, CONCLUSION： We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft.

关键词： ostrich acellular corneal stroma tissue engineering cornea

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Maturation of induced pluripotent stem cell-derived cardiomyocytes and its therapeutic effect on myocardial infarction in mouse

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Bioactive Materials 2023年第2期20卷 286-305页

作者： Peng wu Xiyalatu Sai Zhetao Li Xing Ye Li Jin Guihuan Liu Ge Li pingzhen Yang Mingyi Zhao Shuoji Zhu Nanbo Liu ping Zhu The Second School of Clinical Medicine Southern Medical UniversityGuangzhou510515China Department of Cardiac Surgery Guangdong Cardiovascular InstituteGuangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou510100China Guangdong Provincial Key Laboratory of Pathogenesis Targeted Prevention and Treatment of Heart DiseaseGuangzhou510100China Zhujiang Hospital Southern Medical UniversityGuangzhou510280China Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesSchool of MedicineSouth China University of TechnologyGuangzhou510080China

Induced pluripotent stem cell-derived cardiomyocytes(iPSC-CMs)have an irreplaceable role in the treatment of myocardial infarction(MI),which can be injected into the transplanted area with new cardiomyocytes(Cardiomyocytes,CMs),and improve myocardial ***,the immaturity of the structure and function of iPSC-CMs is the main bottleneck at *** collagen participates in the formation of extracellular matrix(ECM),we synthesized nano colloidal gelatin(Gel)with collagen as the main component,and confirmed that the biomaterial has good biocompatibility and is suitable for cellular in vitro ***,we combined the PI3K/AKT/mTOR pathway inhibitor BEZ-235 with Gel and found that the two combined increased the sarcomere length and action potential amplitude(APA)of iPSC-CMs,and improved the Ca^(2+)processing ability,the maturation of mitochondrial morphological structure and metabolic *** only that,Gel can also prolong the retention rate of iPSC-CMs in the myocardium and increase the expression of Cx43 and angiogenesis in the transplanted area of mature iPSC-CMs,which also provides a reliable basis for the subsequent treatment of mature iPSC-CMs.

关键词： Induced pluripotent stem cell Cardiomyocytes Mature Myocardial infarction treatment

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Development of bone marrow mesenchymal stem cell culture in vitro

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Chinese Medical Journal 2012年第9期125卷 1650-1655页

作者： ZHANG Li PENG Li-pan wu Nan LI Le-ping Department of Gastrointestinal Surgery Provincial Hospital Affiliated to Shandong UniversityJinanShandong 250021China

Objective To review the in vitro development of bone marrow mesenchymal stem cells culture （BM-MSC）.Data sources The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were “bone marrow mesenchymal stem cell” and “cell culture”.Study selection Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional （2D） vs. 3D.Results Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems.Conclusions Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.

关键词： bone marrow mesenchymal stem cells cell culture cell differentiation in vitro

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氢氧化铝有利生长基元[Al_6(OH)_(18)(H_2O)_6]_n微观叠合的DFT计算

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中南大学学报（自然科学版） 2009年第3期40卷 592-600页

作者：吴争平尹周澜陈启元李洁中南大学化学化工学院化学工程与技术博士后流动站湖南长沙410083

以氢氧化铝有利生长基元叠合模型为研究对象,用基于密度泛函理论(DFT)的DMol3程序计算各种典型基元[Al6(OH)18(H2O)6]_n(n=3,4,5,6,7,8)叠合体系的总能量、结合能、基元团间相互作用能及轨道能量等,获得氢氧化铝有利生长基元分子叠合的... 详细信息

以氢氧化铝有利生长基元叠合模型为研究对象,用基于密度泛函理论(DFT)的DMol3程序计算各种典型基元[Al6(OH)18(H2O)6]_n(n=3,4,5,6,7,8)叠合体系的总能量、结合能、基元团间相互作用能及轨道能量等,获得氢氧化铝有利生长基元分子叠合的键力特征等微观图像。研究结果表明,当叠合基元数n相同时,"正面正对叠合"模型的能量稳定性最高,结合力最强;整体上,"正面型"基元团能量的稳定性较高,结合力较强;叠合基元数n对不同叠合方式构建的少基元叠合模型的影响趋势是不同的,对于"侧棱"型叠合,n越大,体系的稳定性越低;对于"正面"型叠合,n越大,体系的稳定性越高;随叠合基元数n的增大,"正面"叠合少基元体系相互吸引的作用力增强,而"侧棱"叠合体系的基元间相互作用能E(VDW)相互排斥的作用力更强;"正面"型叠合是有利于形成较稳定的新相生成基元团的叠合方式,以"正面"叠合为主形成的基元团更有可能形成晶体生长过程中的原始晶胚。

关键词：氢氧化铝有利生长基元微观叠合密度泛函理论

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The expression and binding properties of the rice WRKY68 protein in the Xa21-mediated resistance response to Xanthomonas oryzae pv. Oryzae

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Journal of Integrative Agriculture 2016年第11期15卷 2451-2460页

作者： YANG Shuo ZHOU Liang MIAO Liu-yang SHI Jia-nan SUN Cai-qiang FAN Wei lan Jin-ping CHEN Hao LIU Li-juan DOU Shi-juan LIU Guo-zhen LI Li-yun College of Life Sciences Hebei Agricultural University Baoding 071001 P.R. China Quantum Design China Beijing 100027 P.R.China Beijing Protein Innovation Co. Ltd. Beijing 101318 P.R.China

Plant WRKY transcription factors are involved in various physiological processes, including biotic and abiotic stress responses, as well as developmental processes. In this study, the expression patterns of the WRKY68 protein during interactions between rice 4021 containing the bacterial blight resistance gene Xa21 and Xanthomonas oryzae pv. oryzae（Xoo） were investigated. A possible modified form of the WRKY68 protein appeared in the Xa21-mediated disease resistance response, and its expression levels were similar in compatible and incompatible responses, but differed significantly from that of the mock control treatment, suggesting that WRKY68 may be involved in the bacterial blight response in rice. To further understand WRKY68＇s roles in the resistance signaling pathway, WRKY68 recombinant protein was expressed in Escherichia coli and a microscale thermophoresis analysis was performed to investigate the interactions between WRKY68 and cis-elements in crucial pathogenesis-related（PR） genes. The results showed that the WRKY68 protein binds to W-boxes in the PR1 b promoter region, with an apparent dissociation constant of 25 nmol L–1, while the binding between WRKY68 and PR10 a was W-box independent. The results suggested that a possible modified form of the WRKY68 protein was induced during the interaction between rice and Xoo, which then regulated the activity of the downstream PR genes by binding with the W-boxes in the PR1 b gene＇s promoter region. Moreover, the constitutive transcription of the WRKY68 gene in dozens of rice tissues and the expression of the WRKY68 protein in leaves during all growth stages suggests that WRKY68 plays important roles in rice during normal growth processes.

关键词： rice bacterial blight WRKY transcription factor W-box Western blot microscale thermophoresis

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Changes in the relationship between species richness and belowground biomass among grassland types and along environmental gradients in Xinjiang, Northwest China

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Journal of Arid land 2019年第6期11卷 855-865页

作者： YANG Yuling LI Minfei MA Jingjing CHENG Junhui LIU Yunhua JIA Hongtao LI Ning wu Hongqi SUN Zongjiu FAN Yanmin SHENG Jiandong JIANG ping'an Xinjiang Key Laboratory of Soil and Plant Ecological Processes College of Grassland and Environmental SciencesXinjiang Agricultural UniversityUrumqi 830052China Xinjiang Institute of Ecology and Geography Chinese Academy of SciencesUrumqi 830011China

The association between biodiversity and belowground biomass(BGB) remains a central debate in *** this study, we compared the variations in species richness(SR) and BGB as well as their interaction in the top(0–20 cm), middle(20–50 cm) and deep(50–100 cm) soil depths among 8 grassland types(lowland meadow, temperate desert, temperate desert steppe, temperate steppe desert, temperate steppe, temperate meadow steppe, mountain meadow and alpine steppe) and along environmental gradients(elevation, energy condition(annual mean temperature(AMT) and potential evapotranspiration(PET)), and mean annual precipitation(MAP)) based on a 2011–2013 survey of 379 sites in Xinjiang, Northwest *** SR and BGB varied among the grassland *** alpine steppe had a medium level of SR but the highest BGB in the top soil depth, whereas the lowland meadow had the lowest SR but the highest BGB in the middle and deep soil *** SR and BGB in the different soil depths were tightly associated with elevation, MAP and energy condition;however, the particular forms of trends in SR and BGB depended on environmental factors and soil *** relationship between SR and BGB was unimodal in the top soil depth, but SR was positively related with BGB in the middle soil *** elevation, MAP, energy condition and SR had significant effects on BGB, the variations in BGB in the top soil depth were mostly determined by elevation, and those in the middle and deep soil depths were mainly affected by energy *** findings highlight the importance of environmental factors in the regulations of SR and BGB as well as their interaction in the grasslands in Xinjiang.

关键词： species richness belowground biomass unimodal pattern energy condition soil depths mean annual precipitation grasslands in Xinjiang

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Lower abundance of Bacteroides and metabolic dysfunction are highly associated with the post-weaning diarrhea in piglets

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Science China(Life Sciences) 2022年第10期65卷 2062-2075页

作者： Wen Ren Bing Yu Jie Yu ping Zheng Zhiqing Huang Junqiu Luo Xiangbing Mao Jun He Hui Yan Jinlong wu Daiwen Chen Yuheng Luo Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry of Agriculture of ChinaKey Laboratory of Animal Disease-resistant Nutrition of Sichuan Provinceand Animal Nutrition InstituteSichuan Agricultural UniversityChengdu61l130China DSM(China)Animal Nutrition Research Center Co. LtdBazhou 065799China

Growing evidences show a direct link between diarrhea and disorders of gut microbiota in ***,whether there are microbial markers associated with post-weaning diarrhea remains *** the current study,we compared the microbial community,functions and metabolites between healthy weaned piglets(group H,n=7)and piglets with post-weaning diarrhea(group D,n=7),in order to find out diarrhea associated microbial *** of 7 fecal samples was collected from H and D piglets(weaned at 21 d and sampled at 26 d).The metagenomic and untargeted metabolomic analysis revealed that the microbial composition,function and metabolic profile in D pigs was considerably reshaped,including the reduced abundance and number of Bacteroides,which significantly correlated with the diarrhea status of *** carbohydrate metabolism,biosynthesis and metabolism,lipid metabolism,amino acid metabolism,and the activity of glycan and carbohydrates digestion related enzymes showed extensively down-regulated in D pigs compared with H *** significantly changed the metabolic profiles of fecal microbiota,and most of the altered metabolites were negatively or positively correlated with the change in the abundance of *** conclusion,the lower abundance of Bacteroides and its associated metabolic dysfunction may be regarded as microbial markers of physiological post-weaning diarrhea in piglets.

关键词： post-weaning piglet diarrhea Bacteroides metabolic dysfunction commensal microbiota

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ON INDEFINITE SUBLINEAR ELLIPTIC EQUATIONS

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Systems Science and Mathematical Sciences 2000年第1期13卷 48-58页

作者： YANG Hai tao wu Shao ping (Department of Mathematics, Zhejiang University, Hangzhou 310027, China) 浙江大学数学系浙江杭州 310027 浙江大学数学系浙江杭州

***,N23,beasmoothboundeddomainandconsiderthesemilinearellipticequationswhereatbELoc(Q),***eaction-diffusionprocessess(seethereferencesof[1]).Bytheresultsof[... 详细信息

***,N23,beasmoothboundeddomainandconsiderthesemilinearellipticequationswhereatbELoc(Q),***eaction-diffusionprocessess(seethereferencesof[1]).Bytheresultsof[...

关键词： Sublinear elliptic equation critical point theory sub-sup-solution.

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Study　on　the　damage　of　the　“91．7”　rainstorm　in　Sihu　drainage　area　with　remote　sensing　application　technique

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Journal of Environmental Sciences 1994年第2期6卷 155-163页

作者： Yu Guangming(Department of Geography, Central China Normal Univerrity, wuhan 430070, China)Chen ping wu Xuejin Wang Chaonan(Department of Geography, Xianning Teachero’ College, Xianning 437005, Hubei Province, China)

In this paper, the water equilibrium and ground truth are analyzed for '91.7' rainstormin Sihu drainage area, Hubei Province, China. The flood and waterlogging situation of ' 91.7'rainstorm in this area are investigated using remote sensing, especially the estimation of inundatedarea, the analysis of temporal and spatial characters of this flood and waterlogging damage. Andthe way of flood-trace using remote sensing is explored. At last, the disastroiis conditions are evalu-ated.

关键词： flood and waterlogging damage inundated area remote sensing, rainstorm.

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Association Between Geranylgeranyl Pyrophosphate Synthase Gene Polymorphisms and Bone Phenotypes and Response to Alendronate Treatment in Chinese Osteoporotic Women

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Chinese Medical Sciences Journal 2016年第1期31卷 8-16页

作者： lan-wen Han Dou-dou Ma Xiao-jie Xu Fang Lu Yi Liu Wei-bo Xia Yan Jiang Ou Wang Xiao-ping Xing Mei Li Department of Endocrinology Key Laboratory of Endocrinology of Ministry of HealthPeking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College 100730 Beijing China

Objective To investigate the relationship between geranylgeranyl pyrophosphate synthase （GGPPS） gene polymorphisms and bone response to alendronate in Chinese osteoporotic women. Methods A total of 639 postmenopausal women with osteoporosis or osteopenia were included and randomly received treatment of low dose （70 mg per two weeks） or standard dose （70 mg weekly） of alendronate for one year. The six tag single nucleotide polymorphisms of GGPPS gene were identified. Bone mineral density （BMD）, serum cross-linked C-telopeptide of type I collagen （β-CTX）, and total alkaline phosphatase （ALP） were measured before and after treatment. GGPPS gene polymorphisms and the changes of BMD and bone turnover markers after treatment were analyzed. Results rs10925503 polymorphism of GGPPS gene was correlated to serumβ-CTX levels at baseline, and patients with TT genotype had significantly higher serum β-CTX level than those with TC or CC genotype （all P〈0.05）. No correlation was found between polymorphisms of GGPPS gene and serum total ALP levels, as well as BMD at baseline. After 12 months of treatment, lumbar spine and hip BMD increased and serum bone turnover markers decreased significantly （P〈0.01）, and without obvious differences between the low dose and standard dose groups （all P〉0.05）. However, GGPPS gene polymorphisms were uncorrelated to percentage changes of BMD, serum total ALP, and β-CTX levels （all P〉0.05）. Conclusion GGPPS gene polymorphisms are correlated to osteoclasts activity, but all tag single nucleotide polymorphisms of GGPPS gene have no influence on the skeletal response to alendronate treatment.

关键词： geranylgeranyl pyrophosphateosteoporosis alendronatesynthase tag single nucleotide polymorphisms

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