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Efficacy of Shouzhangshen(Rhizoma Gymnadeniae Crassinervidis)extract against acute high altitude hypoxia-induced brain injury in mice

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Journal of Traditional Chinese Medicine 2021年第4期41卷 546-555页

作者： ZHANG Yongcang LIU Tonghua LIU Lan ZONG Yonghua LI Yu XIONG Hai wu lili Medical College Tibet UniversityLhasa 850000China Department of Chemical Biology School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Tibetan Traditional Medical College Lhasa 850000China Key Laboratory of Health Cultivation of the Ministry of Education Beijing University of Chinese MedicineBeijing 100029China College of Life Sciences Shandong Normal UniversityJinan 250014China

OBJECTIVE:To evaluate the protective effect of Shouzhangshen(Rhizoma Gymnadeniae Crassinervidis)extract against acute high altitude hypoxia-induced brain injury in ***:Sixty C57BL/6J mice were selected and assigned to six groups(n=10):normal control group,low-pressure hypoxia group,positive control group(dexamethasone 500 mg/kg),and three groups treated with Shouzhangshen extract(250,500,and 750 mg/kg,respectively).The Morris water maze test was performed to evaluate alterations in spatial learning and memory *** staining was performed to detect Nissl bodies and neuron ***-inducible factor(HIF)-1α,interleukin(IL)-1β,tumor necrosis factor(TNF)-α,vascular endothelial growth factor(VEGF),and malondialdehyde(MDA)expression in brain tissue and serum,as well as superoxide dismutase(SOD)and glutathione(GSH)activity in brain tissues were measured by enzyme-linked immunosorbent assays,quantitative real-time-polymerase chain reaction and western ***:The Morris water maze test results showed that Shouzhangshen extract can significantly reduce the latency and swimming distance to escape onto a visible platform,increase neuron density and hierarchy and the number of pyramidal neurons,and decrease the expression of HIF-1α,IL-1β,TNF-α,and VEGF mRNAs and proteins in both brain tissue and serum(P0.05).CONCLUSION:Our findings suggest that Shouzhangshen extract may have a significant effect on acute high altitude hypoxia-induced brain injury in mice.

关键词： altitude sickness neuro-protective effect Hypoxia-Inducible Factor 1 alpha Subunit interleukin-1beta tumor necrosis factor-alpha vascular endothelial growth factors Shouzhangshen

Characteristics of fecal microbiota in non-alcoholic fatty liver disease patients

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Science China(Life Sciences) 2018年第7期61卷 770-778页

作者： Fan Li Gang Sun Zikai Wang Wenming wu He Guo Lihua Peng lili wu Xu Guo Yunsheng Yang Department of Gastroenterology and Hepatology Chinese PLA General Hospital Department of Liver Disease PLA Army General Hospital Department of Gastroenterology General Hospital of Jinan Military Region

This study was designed to investigate the gut microbiota of patients with non-alcoholic fatty liver disease. The inclusive and exclusive criteria for NAFLD patients and healthy subjects were formulated, and detailed clinical data were collected. The genomic DNA of stool samples were extracted for 16 S r DNA sequencing, and the amplified V4-region was sequenced on the Illumina Miseq platform. Metastats analysis was performed to identify the differential taxa between the groups. Redundancy analysis was used to evaluate the association between gut microbial structure and clinical variables. Thirty NAFLD patients and37 healthy controls were involved. The 16 S r DNA sequencing showed that there was a dramatic variability of the fecal microbiota among all the individuals. Metastats analysis identified eight families and 12 genera with significant differences between the two groups. When some clinical parameters, such as waist-to-hip ratio(WHR) and homeostasis model assessment of insulin resistance(HOMA-IR), were enrolled in Redundancy analysis, the distribution of the two group of samples was obviously changed. The compositional shifts in fecal bacterial communities of NAFLD patients from the healthy controls were mainly at family or genus levels. According to our Redundancy analysis, insulin resistance and obesity might be closely related to both NAFLD phenotype and intestinal microecology.

关键词： non-alcoholic fatty liver disease bacterial microbiota 16S rDNA

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天然气供应紧张背景下德国的经济适应及启示

中国能源 2024年第4期46卷 90-99页

作者：吴丽丽田磊郭海涛李林郑威中国石油大学(北京)经济管理学院北京102249 中国宏观经济研究院能源研究所北京100038

2022年,乌克兰危机引爆欧洲天然气危机。俄气供给中断后,德国天然气供给缺口迅速扩大。面对全球天然气价格暴涨和俄气断供多重压力,为重新恢复天然气市场供需平衡,德国供给侧快速做出调整,与此同时其需求侧也展现出较大潜力。德国为摆... 详细信息

2022年,乌克兰危机引爆欧洲天然气危机。俄气供给中断后,德国天然气供给缺口迅速扩大。面对全球天然气价格暴涨和俄气断供多重压力,为重新恢复天然气市场供需平衡,德国供给侧快速做出调整,与此同时其需求侧也展现出较大潜力。德国为摆脱对俄气的依赖付出了巨大成本,上至政府下到企业居民都采取了一系列措施以降低此次危机的冲击,并调整经济再平衡:家庭通过调低恒温器来减少天然气需求,而企业则转为使用其他燃料作为天然气的替代品,并进口能源密集型产品。尽管2022年天然气市场深度震荡,但德国通过需求侧调节和替代供应双侧发力,大多数德国工业和家庭天然气用户顺利度过了此次危机。德国成功应对危机,不仅得益于强大的经济适应能力,也得益于欧盟层面的团结与合作,以及各类政策的协调。

关键词：乌克兰危机德国,能源安全经济适应需求侧应对

Effects of GBE50 on hippocampal CA1 synaptic plasticity,learning and memory in an experimental rat model of aging

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Neural Regeneration Research 2011年第12期6卷 892-897页

作者： lili wu Xianwen Dong Gaiying He Zhixiong Zhang Ying Xu Xingyu Wang Yun Li Department of Physiology Shanghai University of Traditional Chinese Medicine Shanghai 201203 China

The content of total flavonoids in an extract of Ginkgo biloba, called GBE50, is 44% by weight. This is significantly greater than that in a standard extract of Ginkgo biloba, designated EGB761. To date, the mechanisms by which GBE50 and EGB761 function remain poorly understood. In the present study, an experimental rat model of aging was induced by intraperitoneal injection of D-galactose, followed by intragastric perfusion of GBE50 （30, 60 mg/kg）, or EGB761 （60 mg/kg）. The water maze scores and hippocampal CA1 synaptic plasticity were evaluated. In the place navigation test, the GBE50 group rats did better than EGB761, while similar scores were obtained in the spatial probe test, and in the platform-switched test. In addition, long-term potentiation was significantly enhanced following high-frequency stimulation in the GBE50 and EGB761 groups, compared with the model group. These results demonstrate that GBE50 and EGB761 improved the learning and memory of aging rats. In particular, GBE50 administered at the 60 mg/kg dose exhibited superior effects over EGB761 at the same 60 mg/kg dose. Furthermore, the enhancement of hippocampal synaptic plasticity may be an underlying mechanism.

关键词： aging GBE 50 memory impairment hippocampus long-term potentiation neural regeneration

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阐述中国传统家具设计的精神内涵

鞋类工艺与设计 2024年第13期4卷 153-155页

作者：吴莉莉张兰英陆艳香桂林电子科技大学艺术与设计学院广西桂林541004 广西培贤国际职业学院广西平果531400

中国传统家具文化历久弥新,从古至今都散发着独特的文化吸引力,同时中国传统家具之美的表现深入渗透到中华民族文化和审美中,具有强烈的美学特征。时代性审美内涵、审美特征以及丰富的语言内涵,都给当代产生了巨大的影响力,而中国传统... 详细信息

中国传统家具文化历久弥新,从古至今都散发着独特的文化吸引力,同时中国传统家具之美的表现深入渗透到中华民族文化和审美中,具有强烈的美学特征。时代性审美内涵、审美特征以及丰富的语言内涵,都给当代产生了巨大的影响力,而中国传统家具是中华民族的历史文脉和重要情节,与社会的各个方面的审美精神与趣味相互促进交融。

关键词：传统家具传统文化精神内涵

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脑CT灌注成像诊断抑郁症患者的价值

辽宁医学杂志 2024年第2期38卷 10-13页

作者：刘亚洲李筠赵志博吴利利周口市中医院河南周口466000

目的探讨脑CT灌注成像诊断抑郁症患者的价值。方法将我院2021年3月至2022年12月期间收治的124例抑郁症患者为观察组,同期选取123例健康体检者作为对照组。所有受试者均进行脑CT灌注成像检查,比较两组颅脑各脑叶CT值、不同脑区脑血流量(r... 详细信息

目的探讨脑CT灌注成像诊断抑郁症患者的价值。方法将我院2021年3月至2022年12月期间收治的124例抑郁症患者为观察组,同期选取123例健康体检者作为对照组。所有受试者均进行脑CT灌注成像检查,比较两组颅脑各脑叶CT值、不同脑区脑血流量(rCBF)、平均通过时间(MTT)及曲线到达峰值时间(TTP)。结果两组左右顶、颞、枕叶增强扫描CT值对比差异无统计学意义(P>0.05),观察组左右额叶CT值低于对照组(P0.05),观察组左右额叶rCBF低于对照组(P0.05),观察组左右额叶MTT长于对照组(P0.05),观察组左右额叶TTP长于对照组(P<0.05)。结论脑CT灌注成像可准确检出抑郁症患者大脑额叶病变,以初步评估患者脑部病变异常,为疾病诊治疗提供一定依据。

关键词：脑CT 灌注成像抑郁症 CT值脑血流量

Studies of new EST-SSRs derived from Gossypium barbadense

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Chinese Science Bulletin 2007年第18期52卷 2522-2531页

作者： ZHANG YanXin LIN ZhongXu LI wu TU lili NIE YiChun ZHANG XianLong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China

Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hir-sutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3-79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3-79) × Emian22), 24 polymor- phic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes. This study effectively proved that EST-SSRs from G. barbadense are valuable for genetic diversity analysis and genetic mapping.

关键词：棉花基因表达基因序列分析遗传多样性基因图谱

稳定“海岛”结构诱导高强度PS/PPO开孔泡沫制备与性能研究

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中国塑料 2024年第3期38卷 49-53页

作者：姜帅华李卓伦芦浩凡孙乙博王向东陈士宏武丽丽北京工商大学轻工科学与工程学院北京100048

采用熔融共混法制备了聚苯乙烯(PS)/聚苯醚(PPO)共混材料。通过控制加工温度,使得PPO以刚性粒子形式存在于共混物中,形成稳定“海岛”结构。以超临界CO_(2)为发泡剂进行了釜压发泡,得到了高开孔率的PS开孔泡沫。研究了PPO含量对PS流变... 详细信息

采用熔融共混法制备了聚苯乙烯(PS)/聚苯醚(PPO)共混材料。通过控制加工温度,使得PPO以刚性粒子形式存在于共混物中,形成稳定“海岛”结构。以超临界CO_(2)为发泡剂进行了釜压发泡,得到了高开孔率的PS开孔泡沫。研究了PPO含量对PS流变性能、热力学行为的影响以及稳定“海岛”结构对形成PS开孔泡沫的影响,对所得PS/PPO泡沫制品的泡孔尺寸、发泡倍率、开孔率及泡沫压缩性能进行了分析。结果表明,当发泡温度为158℃、压力为20 MPa、PPO含量为10份(质量份,下同)时,可以制备得到发泡倍率为37.5倍、开孔率为96.9%的PS开孔泡沫。

关键词：聚苯乙烯聚苯醚超临界二氧化碳发泡材料开孔泡沫

血红素加氧酶⁃1通过调控bHLH亮氨酸拉链转录因子E3/高尔基体应激反应减轻小鼠内毒素性急性肺损伤

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国际麻醉学与复苏杂志 2024年第1期45卷 11-17页

作者：张野李香云黄炎吴丽丽武丽娜余剑波天津医科大学南开临床学院(天津市南开医院)麻醉与重症医学科天津300100

目的探讨在小鼠内毒素性急性肺损伤(ALI)中血红素加氧酶⁃1(HO⁃1)对bHLH亮氨酸拉链转录因子E3(TFE3)表达与核转位以及高尔基体应激反应的影响。方法将24只雄性C57BL/6小鼠按随机数字表法分为4组(每组6只):空白对照组(Ctrl组)、内毒素[脂... 详细信息

目的探讨在小鼠内毒素性急性肺损伤(ALI)中血红素加氧酶⁃1(HO⁃1)对bHLH亮氨酸拉链转录因子E3(TFE3)表达与核转位以及高尔基体应激反应的影响。方法将24只雄性C57BL/6小鼠按随机数字表法分为4组(每组6只):空白对照组(Ctrl组)、内毒素[脂多糖(lipopolysaccharide,LPS)]急性肺损伤组(LPS组)、内毒素性急性肺损伤+HO⁃1激动剂氯高铁血红素(Hemin)组(LPS+Hemin组)和Hemin组。Ctrl组尾静脉注射生理盐水0.5 ml;LPS组尾静脉注射LPS 10 mg/kg建立小鼠内毒素性ALI模型;LPS+Hemin组腹腔注射Hemin 50 mg/kg,1 h后尾静脉注射LPS 10 mg/kg建立内毒素性ALI模型;Hemin组腹腔注射Hemin 50 mg/kg。造模12 h后对小鼠进行断颈处死,收取肺组织。苏木精⁃伊红染色(H⁃E染色)观察小鼠肺组织病理学变化并行肺损伤评分;计算肺组织湿重/干重(W/D)值;脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测肺组织细胞凋亡指数;酶联免疫吸附测定(ELISA)法检测肺组织白细胞介素(IL)⁃6、肿瘤坏死因子(TNF)⁃α含量;流式细胞仪检测肺组织活性氧(ROS)的含量;免疫荧光染色观察TFE3核转位情况;蛋白质免疫印迹法(Western blot)测定HO⁃1、TFE3、高尔基体基质蛋白130(GM130)、高尔基体重组和堆叠蛋白65(GRASP65)、囊泡转运蛋白小GTP酶20(RAB20)、突触融合蛋白3(STX3A)、WD重复结构域与磷酸肌醇相互作用蛋白1(WIPI1)的表达水平。结果与Ctrl组比较,LPS组小鼠肺组织病理学损伤加重,肺损伤评分、W/D值及细胞凋亡指数升高,ROS、IL⁃6及TNF⁃α含量明显升高,TFE3表达及核转位增多,HO⁃1、GRASP65、RAB20、STX3A的表达水平增加,WIPI1、GM130表达水平减少(均P0.05)。与LPS组比较,LPS+Hemin组小鼠肺组织病理学损伤减轻,肺损伤评分、W/D值及细胞凋亡指数下降,ROS、IL⁃6及TNF⁃α含量减少,TFE3表达及核转位减少,HO⁃1、WIPI1、GM130表达水平增加,GRASP65、RAB20、STX3A表达水平减少(均P<0.05)。结论HO⁃1能够减轻内毒素诱导的小鼠ALI,其机制可能与其调控TFE3表达、核转位及高尔基体应激反应有关。

关键词：内毒素血症急性肺损伤血红素加氧酶⁃1 高尔基体 bHLH亮氨酸拉链转录因子E3