Studies of new EST-SSRs derived from Gossypium barbadense

作     者：ZHANG YanXin LIN ZhongXu LI Wu TU LiLi NIE YiChun ZHANG XianLong 

作者机构：National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2007年第52卷第18期

页      面：2522-2531页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Support from the Ministry of Science and Technology of China (973 Project, Grant No. 2004 CB117301) the National Natural Science Foundation of China (Grant No. 30600396) 

主　　题：棉花 基因表达 基因序列分析 遗传多样性 基因图谱 

摘      要：Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hir-sutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3-79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3-79) × Emian22), 24 polymor- phic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes. This study effectively proved that EST-SSRs from G. barbadense are valuable for genetic diversity analysis and genetic mapping.