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Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis

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Synthetic and Systems Biotechnology 2018年第4期3卷 236-243页

作者： Jiang Zhu dongbo Cai Haixia Xu Ziwei Liu Bowen Zhang Fei wu Junhui Li Shouwen Chen Environmental Microbial Technology Center of Hubei Province Hubei Collaborative Innovation Center for Green Transformation of Bio-ResourcesState Key Laboratory of Biocatalysis and Enzyme EngineeringCollege of Life SciencesHubei UniversityWuhan430062PR China Lifecome Biochemistry Co.Ltd Nanping353400PR China

Bacitracin,a new type of cyclic peptide antibiotic,is widely used as the feed additive in feed *** chain amino acids(BCAAs)are the key precursors for bacitracin *** this research,soybean meal was served as the raw material to supply precursor amino acids for bacitracin synthesis,and enhanced production of bacitracin was attempted by engineering BCAA transporter BrnQ and its regulator Lrp in the bacitracin industrial production strain Bacillus licheniformis ***,our results confirmed that Lrp negatively affected bacitracin synthesis in DW2,and deletion of lrp improved intracellular BCAA accumulations,as well as the expression level of BCAA transporter BrnQ,which further led to a 14.71%increase of bacitracin yield,compared with that of *** the contrary,overexpression of Lrp decreased bacitracin yield by 12.28%.Secondly,it was suggested that BrnQ acted as a BCAA importer in DW2,and overexpression of BrnQ enhanced the intracellular BCAA accumulations and 10.43%of bacitracin ***,the bacitracin yield decreased by 18.27%in the brnQ deletion strain DW2△***,BrnQ was further overexpressed in lrp deletion strain DW2△lrp,and bacitracin yield produced by the final strain DW2△lrp::BrnQ was 965.34 U/mL,increased by 22.42%compared with that of DW2(788.48 U/mL).Collectively,this research confirmed that Lrp affected bacitracin synthesis via regulating the expression of BCAA transporter BrnQ and BCAA distributions,and provided a promising strain for industrial production of bacitracin.

关键词： Bacillus licheniformis Bacitracin Regulator Lrp BCAA transporter BrnQ Branched chain amino acid

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Smart Substation Integration Technology and Its Application in Distribution Power Grid

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CSEE Journal of Power and Energy Systems 2016年第4期2卷 31-36页

作者： Qipeng Song Wanxing Sheng Lingfeng Kou dongbo Zhao Ziping wu Hengfu Fang Xiaolong Zhao China Electric Power Research Institute Beijing 100192China Eaton Corporation Eden PrairieMN55344USA University of Denver DenverCOUSA

At present,smart substations use the IEC61850 standard based on the architectural framework of three layers and two networks to realize information digitization and advanced *** the smart substation offers many improvements in design,equipment manufacturing,and construction,the intelligent devices used in smart substations are costly,and are also difficult to maintain since they are dispersed within a single *** optimization and device integration,thus,have become important issues in smart substation *** paper presents an integrated solution and implementation process for a smart substation *** the process layer,an integrated intelligence component is developed that functions both as an intelligent terminal and a merging *** the bay layer,an integrated station-area protection measurement and control master device is designed to achieve such functions as protection,monitoring,control,fault recorder,and power quality ***,in the station control layer,an integrated information platform is established to bring together various system functions and to promote interactive *** technology improves the economy and practicality of the smart substation,especially in a distribution power grid.

关键词： Equipment integration integrated intelligent components smart substation substation-area monitoring and protection three layers and two networks

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Vitamin B3 inhibits apoptosis and promotes autophagy of isletβcells under high glucose stress

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BIOCELL 2023年第4期47卷 859-868页

作者： YU ZHANG XI’AN ZHOU CHUNYAN ZHANG DENGNI LAI dongbo LIU YANYANG wu Key Laboratory for Food Science and Biotechnology of Hunan Province College of Food Science and TechnologyHunan Agricultural UniversityChangsha410128China Horticulture and Landscape College Hunan Agricultural UniversityChangsha410128China Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients Changsha410128China State Key Laboratory of Subhealth Intervention Technology Changsha410128China Hunan Agricultural Products Processing Institute Hunan Food Test and Analysis CenterChangsha410004China

Background:Hyperglycemia is a typical symptom of *** glucose induces apoptosis of isletβ*** autophagy functions in cytoprotection and autophagic cell *** interaction between autophagy and apoptosis is important in the modulation of the function of isletβ*** B3 can induce autophagy and inhibit isletβ***:The mechanism of vitamin B3-mediated protective effect on the function of isletβcells was explored by the method of western blot,immunofluorescence and flow ***:In the present study,high glucose stress increased the apoptosis rate,while vitamin B3 reduced the apoptosis *** effect of vitamin B3 on autophagy flux under normal and high glucose stress was also *** B3 increased the number of autophagosomes and increased the light chain(LC)3-II/LC3-I *** contrast,vitamin B3 decreased sequestosome 1(SQSTM1)/p62 protein expression and inhibited the phosphorylation of mammalian ribosomal protein S6 kinaseβ-1(p70S6K/S6K1),which was a substrate of mammalian target of rapamycin(mTOR)under normal and high glucose *** further verify the protective effect of vitamin B3 on apoptosis,we treated isletβcell RIN-m5F with autophagy inhibitor 3-methyladenine(3-MA).Vitamin B3 decreased the apoptosis rate under high glucose stress,while the inhibition of apoptosis by vitamin B3 was blocked after adding ***:Our data suggested that vitamin B3 reduced the apoptosis rate ofβcells,possibly through inducing autophagy under high glucose stress.

关键词： Vitamin B3 High glucose Autophagy Apoptosis

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定位针引导下腹腔镜精准局部肝切除应用探索

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中华腔镜外科杂志（电子版） 2024年第2期17卷 125-128页

作者：陈政叶庆旺赵东波石鑫吴建强余德才南京大学医学院附属鼓楼医院普通外科肝胆与肝移植外科江苏210008 南京鼓楼医院集团宿迁医院、徐州医科大学附属宿迁医院肝胆外科江苏223800

目的探索定位针引导腹腔镜下深部肝肿瘤局部切除的安全性与可行性。方法回顾分析2021年6月至2022年12月6例经术前超声或CT定位深部肝肿瘤行腹腔镜下肝肿瘤局部切除患者的病历资料,评估穿刺针定位的安全性、荷瘤靶域切除的精准性和R0切... 详细信息

目的探索定位针引导腹腔镜下深部肝肿瘤局部切除的安全性与可行性。方法回顾分析2021年6月至2022年12月6例经术前超声或CT定位深部肝肿瘤行腹腔镜下肝肿瘤局部切除患者的病历资料,评估穿刺针定位的安全性、荷瘤靶域切除的精准性和R0切除率。结果1例患者2个病灶行超声定位、5例患者行CT定位。6例患者7个病灶定位确切、锚定可靠,无松脱、移位,定位引起出血5~30(13.2±9.8)ml;切缘0.9~2.5(1.9±0.5)cm,R0切除率100%。结论定位针定位准确可靠、安全可行,能起到良好的定位、引导作用,是特定条件下术中超声和荧光腔镜的有益补充。

关键词：定位针腹腔镜肝切除术肝细胞癌肿瘤定位影像学

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新辅助放化疗联合全直肠系膜切除术治疗局部进展期直肠癌的疗效及安全性分析

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临床普外科电子杂志 2022年第1期10卷 72-76页

作者：尚小文吴东波宋学民谢德智徐君毅汤阳阳罗文于鹏程广西医科大学第四附属医院胃肠外科广西柳州545000

目的分析新辅助放化疗联合全直肠系膜切除术治疗局部进展期直肠癌的疗效及安全性。方法回顾性分析广西医科大学第四附属医院2017年6月至2020年6月收治并行全直肠系膜切除术的局部进展期直肠癌患者的临床资料,共100例,按是否行新辅助... 详细信息

目的分析新辅助放化疗联合全直肠系膜切除术治疗局部进展期直肠癌的疗效及安全性。方法回顾性分析广西医科大学第四附属医院2017年6月至2020年6月收治并行全直肠系膜切除术的局部进展期直肠癌患者的临床资料,共100例,按是否行新辅助放化疗(neoadjuvant chemoradiotherapy,nCRT)分为nCRT组(50例)和非n CRT组(50例)。比较两组患者治疗后的临床指标、术后并发症及对比新辅助放化疗前后肿瘤TNM分期。结果新辅助放化疗后肿瘤分期明显下降。nCRT组患者新辅助放化疗后的术中出血量、保护性造口率高于非nCRT组,手术时间、术后住院时间长于非nCRT组,淋巴结清扫数及保肛率低于非nCRT组,差异均有显著性(P 0.05)。结论新辅助放化疗联合全直肠系膜切除术治疗虽然增加直肠癌患者的保护性造口率、延长手术时间、增加手术出血,但可明显降低肿瘤分期。

关键词：新辅助放化疗全直肠系膜切除术局部进展期直肠癌临床疗效安全性

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基于知识图谱的伤寒论课程学习资源推荐模型

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现代信息科技 2024年第16期8卷 182-188页

作者：楚龙翔陶涛陈国龙易宇翔吴豪威刘东波湖南中医药大学信息科学与工程学院湖南长沙410208

结合知识图谱和个性化资源推荐算法,实现伤寒论课程学习资源的个性化推荐,帮助学习者更有效地学习。利用前期研究的知识图谱,融合课程本体知识,构建伤寒论课程知识图谱。在此基础上,结合协同过滤和基于路径的推荐算法,设计混合推荐算法... 详细信息

结合知识图谱和个性化资源推荐算法,实现伤寒论课程学习资源的个性化推荐,帮助学习者更有效地学习。利用前期研究的知识图谱,融合课程本体知识,构建伤寒论课程知识图谱。在此基础上,结合协同过滤和基于路径的推荐算法,设计混合推荐算法,按相似度排序推荐学习资源给用户。提供形式化的知识表示方法和组织模型,满足个性化学习需求,为学习者提供符合其学习路线的资源,实现精准推送。该模型能够有效地解决伤寒论课程教学中存在的“晦涩难读懂、枯燥难专注、教学难互动”的问题,有助于学习者更好地学习伤寒论课程。

关键词：课程知识图谱伤寒论资源推荐算法个性化学习

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基于领域大语言模型的古籍分词研究

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科技情报研究 2024年第2期6卷 11-20页

作者：朱丹浩赵志枭吴娜王希羽孙光耀王东波江苏警官学院刑事科学技术系南京210031 南京农业大学信息管理学院南京210095

[目的/意义]文章以古籍自动分词为切入点,引入“荀子”系列大语言模型,对大语言模型在古籍文本分词任务上的表现进行了探讨。[方法/过程]文章基于《左传》分词语料,进行了数据清洗和整理,构建了指令数据集,在此基础上,从数据集中抽取了1... 详细信息

[目的/意义]文章以古籍自动分词为切入点,引入“荀子”系列大语言模型,对大语言模型在古籍文本分词任务上的表现进行了探讨。[方法/过程]文章基于《左传》分词语料,进行了数据清洗和整理,构建了指令数据集,在此基础上,从数据集中抽取了1 000条作为测试数据,并分别使用500、1 000、2 000、5 000条数据作为训练数据进行指令微调,并测试其性能。[结果/结论]实验结果表明,只需要少量的数据,大语言模型就可以有较为理想的表现,在微调数据量达到5 000条数据时,Xunzi-Qwen-7B模型表现出了最优性能,F1值达到84.54%。

关键词： “荀子”大模型《左传》分词指令微调

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Monovalent cation perm-selective membranes(MCPMs)"New developments and perspectives

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Chinese Journal of Chemical Engineering 2017年第11期25卷 1606-1615页

作者： Liang Ge Bin wu dongbo Yu Abhishek N.Mondal Linxiao Hou Noor U1 Afsar Qiuhua Li Tingting Xu Jibin Miao Tongwen Xu CAS Key Laboratory of Soft Matter Chemistry Collaborative Innovation Center of Chemistry for Energy MaterialsSchool of Chemistry and Materials ScienceUniversity of Science and Technology of ChinaHefei 230026China

As one of the most typical and promising membrane processes, electrodialysis(ED) technique plays a more and more significant role in industrial separation. Especially, the separation of monovalent cations and multivalent cations is currently a hot topic, which is not only desirable for many industries but also challenging for academic explorations. The main aim of the present contribution is to view the advances of a wide variety of monovalent cation perm-selective membranes(MCPMs) and their preparation technologies including(1) covalent crosslinking,(2) surface modification,(3) polymer blending,(4) electrospinning,(5) nanofiltration alike membrane,and(6) organic–inorganic hybrid. The relevant advantages and disadvantages with respect to some specific cases have been discussed and compared in detail. Furthermore, we elaborately discuss the opportunities and challenges of MCPMs, the fabricating strategies to take and the future perspectives.

关键词： Ion exchange membranes Monovalent cation perm-selective membranes Electrodialysis Ion separation Electro-nanofiltration

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Metabolic engineering of Bacillus amyloliquefaciens for efficient production ofα-glucosidase inhibitor1-deoxynojirimycin

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Synthetic and Systems Biotechnology 2023年第3期8卷 378-385页

作者： Xujie Li Meng Zhang Yu Lu Ningyang wu Jian’gang Chen Zhixia Ji Yangyang Zhan Xin Ma Junyong Chen dongbo Cai Shouwen Chen State Key Laboratory of Biocatalysis and Enzyme Engineering Environmental Microbial Technology Center of Hubei ProvinceCollege of Life SciencesHubei UniversityWuhan430062China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan430070China Department of Urology Zhuhai People’s Hospital(Zhuhai Hospital Affiliated to Jinan University)Zhuhai519000China wuhan Jun’an Biotechnology Co. Ltd.Wuhan430070China

Owing to the feature of strongα-glucosidase inhibitory activity,1-deoxynojirimycin(1-DNJ)has broad application prospects in areas of functional food,biomedicine,etc.,and this research wants to construct an efficient strain for 1-DNJ production,basing on Bacillus amyloliquefaciens ***,using the temperature-sensitive shuttle plasmid T2(2)-Ori,gene ptsG in phosphotransferase system(PTS)was weakened by homologous recombination,and non-PTS pathway was strengthened by deleting its repressor gene iolR,and 1-DNJ yield of resultant strain HZ-S2 was increased by 4.27-fold,reached 110.72 mg/***,to increase precursor fructose-6-phosphate(F-6-P)supply,phosphofructokinase was weaken,fructose phosphatase GlpX and 6-phosphate glucose isomerase Pgi were strengthened by promoter replacement,moreover,regulator gene nanR was deleted,1-DNJ yield was further increased to 267.37 mg/L by ***,promoter of 1-DNJ synthetase cluster was optimized,as well as 5′-UTRs of downstream genes in synthetase cluster,and 1-DNJ produced by the final strain reached 478.62 mg/*** but not the least,1-DNJ yield of 1632.50 mg/L was attained in 3 L fermenter,which was the highest yield of 1-DNJ reported to *** together,our results demonstrated that metabolic engineering was an effective strategy for 1-DNJ synthesis,this research laid a foundation for industrialization of functional food and drugs based on 1-DNJ.

关键词： 1-Deoxynojirimycin Bacillus amyloliquefaciens Metabolic engineering Glucose transport pathway Fructose 6-phosphate 1-DNJ synthetase Gene cluster

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奥马珠单抗对过敏性鼻炎合并中度持续性支气管哮喘的疗效观察

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中国实用医刊 2024年第14期51卷 76-80页

作者：马东波王喜玲张辉伍冬冬史向蕊王梦瑶吴秋歌郑州大学第一附属医院呼吸与危重症医学科郑州450052

目的探讨奥马珠单抗对过敏性鼻炎合并中度持续性支气管哮喘的临床疗效。方法回顾性分析于郑州大学第一附属医院接受治疗的167例过敏性鼻炎合并中度持续支气管哮喘患者的临床资料。167例患者中,82例给予中剂量ICS/LABA类药物(布地奈德福... 详细信息

目的探讨奥马珠单抗对过敏性鼻炎合并中度持续性支气管哮喘的临床疗效。方法回顾性分析于郑州大学第一附属医院接受治疗的167例过敏性鼻炎合并中度持续支气管哮喘患者的临床资料。167例患者中,82例给予中剂量ICS/LABA类药物(布地奈德福莫特罗吸入粉雾剂或沙美特罗替卡松吸入气雾剂)联合奥马珠单抗治疗者为观察组,85例给予高剂量布地奈德福莫特罗吸入粉雾剂或沙美特罗替卡松吸入气雾剂治疗者为对照组。评估两组各时间点相关检测指标[血免疫球蛋白E(IgE)、外周血嗜酸性粒细胞(EOS),呼出气一氧化氮(FeNO)、哮喘控制测试评分(ACT)]、肺功能指标[第1秒用力呼气容积(FEV1)、第1秒用力呼气容积占预计值的百分比(FEV1%pred)、第1秒用力呼气容积占用力肺活量的百分比(FEV1/FVC)]及鼻部症状缓解情况。结果对照组治疗3个月IgE、FeNO水平低于治疗前,ACT、FEV1、FEV1/FVC水平高于治疗前(P<0.05),对照组治疗后12个月IgE、FeNO水平低于治疗前,ACT、FEV1、FEV1%pred、FEV1/FVC水平高于治疗前(P<0.05);对照组治疗12个月FeNO水平低于治疗3个月,FEV1、FEV1%pred、FEV1/FVC水平高于治疗3个月,鼻部症状缓解情况优于治疗3个月(P<0.05)。观察组治疗3、12个月IgE及FeNO水平低于治疗前,ACT、FEV1、FEV1%pred、FEV1/FVC水平高于治疗前(P<0.05);观察组治疗12个月IgE、FeNO水平低于治疗后3个月,ACT、FEV1、FEV1%pred、FEV1/FVC水平高于治疗后3个月,鼻部症状缓解优于治疗后3个月(P<0.05)。治疗3个月,观察组FeNO水平低于对照组,ACT、FEV1、FEV1/FVC水平高于对照组(P<0.05);治疗12个月,观察组IgE、FeNO水平低于对照组,ACT、FEV1、FEV1%pred、FEV1/FVC水平高于对照组,鼻部症状缓解优于对照组(P<0.05)。结论高剂量ICS/LABA类药物单独使用,以及中剂量ICS/LABA类药物联合奥马珠单抗于治疗3、12个月,患者呼吸功能及鼻部症状均有所缓解,但联合用药随着治疗时间的延长鼻部症状缓解、肺功能改善更明显。

关键词：奥马珠单抗过敏性鼻炎支气管哮喘临床疗效

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