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Gynoecy instability in cucumber(Cucumis sativus L.)is due to unequal crossover at the copy number variation-dependent Femaleness(F)locus

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Horticulture Research 2020年第1期7卷 2342-2356页

作者： Zheng Li Yonghua Han Huanhuan Niu Yuhui Wang Biao Jiang yiqun weng Horticulture Department University of WisconsinMadisonWI 53706USA College of Horticulture Northwest A&F UniversityYanglingShaanxi 712100China Institute of Integrative Plant Biology School of Life SciencesJiangsu Normal UniversityXuzhou 221116China Vegetable Research Institute Guangdong Academy of Agricultural ScienceGuangzhouGuangdong 510640China USDA-ARS Vegetable Crops Research UnitMadisonWI 53706USA

Cucumber,Cucumis sativus is an important vegetable crop,and gynoecy has played a critical role in yield increase of hybrid cucumber *** has a unique genetic system for gynoecious sex expression,which is determined by the copy number variation(CNV)-based,dominant,and dosage-dependent femaleness(F)***,this gynoecy expression system seems unstable since monecious plants could often be found in Fdependent gynoecious cucumber *** hypothesized that gynoecy instability(gynoecy loss)may be due to unequal crossing over(UCO)during meiosis among repeat units of the *** this study,using high throughput genome resequencing,fiber-FISH and genomic qPCR analyses,we first confirmed and refined the structure of the F locus,which was a CNV of a 30.2-kb tandem *** plants contained three genes:CsACS1,CsACS1G,and CsMYB,of which CsACS1G is a duplication of CsACS1 but with a recombinant distal promoter that may contribute to gynoecy sex *** two large populations from self-pollinated gynoecious inbred lines,‘gynoecy loss’mutants were identified with similar mutation rates(~0.12%).We show that these monecious mutants have lost *** addition,we identified gynoecious lines in natural populations that carry two copies of *** proposed a model to explain gynoecy instability in F-dependent cucumbers,which is caused by UCO among CSACS1/G units during *** findings present a convincing case that the phenotypic variation of an economically important trait is associated with the dynamic changes of copy numbers at the F *** work also has important implications in cucumber breeding.

关键词： fir number dosage

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Horticulture Research 2020年第1期7卷 434-448页

作者： Hu Wang Huanhuan Niu Chuang Li Guoyan Shen Xiaofeng Liu yiqun weng Tao Wu Zheng Li State Key Laboratory of Crop Stress Biology in Arid Areas College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China Department of Vegetable Sciences Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsChina Agricultural UniversityBeijing 100193China USDA-ARS Vegetable Crops Research UnitHorticulture DepartmentUniversity of WisconsinMadisonWI 53706USA College of Horticulture and Landscape Architecture Hunan Agricultural UniversityChangshaHunan 410128China

In plants,WUSCHEL-related homeobox1(WOX1)homologs promote lamina mediolateral ***,the downstream components linking WOX1 and lamina development remain *** this study,we revealed the roles of WOX1 in palmate leaf expansion in cucumber(Cucumis sativus).A cucumber mango fruit(mf)mutant,resulting from truncation of a WOX1-type protein(CsWOX1),displayed abnormal lamina growth and defects in the development of secondary and smaller ***1 was expressed in the middle mesophyll and leaf margins and rescued defects of the Arabidopsis wox1 prs double *** analysis revealed that genes involved in auxin polar transport and auxin response were highly associated with leaf *** of the cucumber mf rl(round leaf)double mutant revealed that CsWOX1 functioned in vein development via PINOID(CsPID1)-controlled auxin *** of CsWOX1 in cucumber(CsWOX1-OE)affected vein patterning and produced‘butterfly-shaped’***1 physically interacted with CsTCP4a,which may account for the abnormal lamina development in the mf mutant line and the smaller leaves in the CsWOX1-OE *** findings demonstrated that CsWOX1 regulates cucumber leaf vein development by modulating auxin polar transport;moreover,CsWOX1 regulates leaf size by controlling CIN-TCP genes.

关键词： size WUS shaped

A Sequencing-Based Linkage Map of Cucumber

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Molecular Plant 2015年第6期8卷 961-963页

作者： Zhou, Qian Miao, Han Li, Shuai Zhang, Shengping Wang, Ye weng, yiqun Zhang, Zhonghua Huang, Sanwen Gu, Xingfang Chinese Acad Agr Sci Key Lab Biol & Genet Improvement Hort Crops Inst Vegetables & Flowers Minist AgrSino Dutch Joint Lab Hort Gen Beijing 10081 Peoples R China Chinese Acad Agr Sci Agr Genom Inst Shenzhen Shenzhen 518120 Peoples R China Univ Wisconsin Vegetable Crops Res Unit Dept Hort USDAARS Madison WI 53706 USA

Cucumber （Cucumis sativus L., 2n = 2x = 14） is an economically important vegetable worldwide and has served as a model sys- tem for plant vascular biology and sex determination studies. We reported the draft genome assembly of the North China type cucumber line 9930 in 2009 （Huang et al., 2009a） and constructed a single-base resolution map of cucumber genomic variation by resequencing of the genomes of 115 core germplasm lines （Qi et al., 2013）, which enabled genome-wide association studies of important traits in cucumber （Shang et al., 2014）.

关键词： Sequencing-Based Linkage Map

Efficient Transposition of the Retrotransposon Tnt1 in Cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2018年第3期4卷 111-116页

作者： ZHANG Qi DU Hui LV Duo XIAO Tingting PAN Jian HE Huanle WANG Gang CAI Run weng yiqun PAN Junsong Plant Science Department School of Agriculture and BiologyShanghai Jiao Tong University USDA-ARS Vegetable crops Research UnitHorticulture DepartmentUniversity of Wisconsin

Tnt1 is an active retrotransposon originally identified in tobacco(Nicotiana tabacum L.)(Grandbastien et al.,1989),but its transposition activity could be activated through tissue culture in other plant *** insertions are stable and inheritable in the progeny,which has made it a valuable and versatile tool for developing insertional mutagenesis libraries in several plant ***,we explored its utility for mutagenesis in cucumber(Cucumis sativus L.).T_3 Tnt1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated *** PCR and analyses and Southern hybridization,we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this *** genome walking,some flanking sequences of Tnt1 insertions were recovered in regenerated *** results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber and that it is feasible to use for developing an insertional mutagenesis library for cucumber.

关键词： cucumber Tnt1 retrotransposon tissue culture mutagenesis library

维普期刊数据库

Melon short internode(CmSi)encodes an ERECTA-like receptor kinase regulating stem elongation through auxin signaling

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Horticulture Research 2020年第1期7卷 269-282页

作者： Sen Yang Kaige Zhang Huayu Zhu Xiaojing Zhang Wenkai Yan Nana Xu Dongming Liu Jianbin Hu Yufeng Wu yiqun weng Luming Yang College of Horticulture Henan Agricultural University63 Nongye Road450002 ZhengzhouChina State Key Laboratory of Crop Genetics and Germplasm Enhancement Bioinformatics CenterNanjing Agricultural University210095 NanjingChina USDA-ARS Vegetable Crops Research UnitHorticulture DepartmentUniversity of Wisconsin–MadisonMadisonWI 53706USA

Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic *** least four dwarf/semidwarf genes have been identified in different melon varieties,but none of them have been cloned,and little is known about the molecular mechanisms underlying internode elongation in ***,we report map-based cloning and functional characterization of the first semidwarf gene short internode(Cmsi)in melon,which encodes an ERECTA-like receptor kinase regulating internode ***-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode *** expression level of CmSI was positively correlated with stem length in the different melon varieties *** expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both *** quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant,including several well-known auxin transporters,such as members of the ABCB family and PINFORMED genes.A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin ***,CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in *** findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling,which can directly affect polar auxin transport.

关键词： species dwarf traits

A Truncated F-Box Protein Confers the Dwarfism in Cucumber

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Journal of Genetics and Genomics 2016年第4期43卷 223-226页

作者： Tao Lin Shenhao Wang Yang Zhong Dongli Gao Qingzhi Cui Huiming Chen Zhonghua Zhang Huolin Shen yiqun weng Sanwen Huang Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural GenomicsInstitute of Vegetables and FlowersChinese Academy of Agricultural Sciences Department of Horticulture China Agricultural University Agricultural Genome Institute at Shenzhen Chinese Academy of Agricultural Sciences Hunan Vegetable Research Institute Hunan Academy of Agricultural Sciences USDA-ARS Vegetable Crops Research UnitDepartment of HorticultureUniversity of Wisconsin

Dwarfism is an important plant architecture trait in crop breeding（Peng et al.,1999;Sasaki el al.,2002）.In *** compact plant type was proposed to develop new varieties for the once-over mechanical harvest for concen... 详细信息

关键词： gene SNPs A Truncated F-Box Protein Confers the Dwarfism in Cucumber length

维普期刊数据库

Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature

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Horticulture Research 2020年第1期7卷 2678-2697页

作者： Yuhui Wang Kailiang Bo Xingfang Gu Junsong Pan Yuhong Li Jinfeng Chen Changlong Wen Zhonghai Ren Huazhong Ren Xuehao Chen Rebecca Grumet yiqun weng Department of Horticulture University of WisconsinMadisonWI 53706USA Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China Department of Plant Sciences Shanghai Jiaotong UniversityShanghai 200240China Horticulture College Northwest A&F UniversityYangling 712100China Horticulture College Nanjing Agricultural UniversityNanjing 210095China Beijing Vegetable Research Center Beijing Academy of Agricultural and Forestry SciencesBeijing 100097China College of Horticulture Science and Engineering Shandong Agricultural UniversityTai’an 271018China College of Horticulture China Agricultural UniversityBeijing 100193China College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China Department of Horticulture Michigan State UniversityEast LansingMI 48824USA USDAARS Vegetable Crops Research Unit 1575 Linden Dr.MadisonWI 53706USA

Cucumber,Cucumis sativus L.(2n=2x=14),is an important vegetable crop *** was the first specialty crop with a publicly available draft *** relatively small,diploid genome,short life cycle,and selfcompatible mating system offers advantages for genetic *** recent years,significant progress has been made in molecular mapping,and identification of genes and QTL responsible for key phenotypic traits,but a systematic review of the work is ***,we conducted an extensive literature review on mutants,genes and QTL that have been molecularly mapped or characterized in *** documented 81 simply inherited trait genes or major-effect QTL that have been cloned or fine *** each gene,detailed information was compiled including chromosome locations,allelic variants and associated polymorphisms,predicted functions,and diagnostic markers that could be used for marker-assisted selection in cucumber *** also documented 322 QTL for 42 quantitative traits,including 109 for disease resistances against seven *** alignment of these QTL on the latest version of cucumber draft genomes,consensus QTL across multiple studies were inferred,which provided insights into heritable correlations among different *** collaborative efforts among public and private cucumber researchers,we identified 130 quantitative traits and developed a set of recommendations for QTL nomenclature in *** is the first attempt to systematically summarize,analyze and inventory cucumber mutants,cloned or mapped genes and QTL,which should be a useful resource for the cucurbit research community.

关键词： trait traits cucumber

Chromosome-specific painting and pachytene mapping of homoeologous chromosome pairing in Cucumis species using bulked oligonucleotides

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Chromosome-specific painting and pachytene mapping of homoeo...

江苏省遗传学会第九次会员代表大会暨学术研讨会

作者： Yonghua Han Tao Zhang Paradee Thammapichai yiqun weng Jiming Jiang Department of Horticulture University of Wisconsin-Madison The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province Jiangsu Normal University US Department of Agriculture/Agricultural Research Service Vegetable Crops Research Unit

Chromosome-specific painting is a powerful technique in molecular cytogenetic and genome research. We developed an oligonucleotide（oligo） chromosome-painting technique in Cucumis species that will be applicable in any plant species with a sequenced genome. A bioinformatic pipeline was developed to select oligos specific to a single chromosome of cucumber（Cucumis sativus）. A massively parallel de novo synthesis approach was used to synthesize all selected oligos. The synthesized oligos were then amplified and labeled by biotin or digoxigenin for fluorescence in situ hybridization. We developed three probes with each containing 23,000 to 27,000 oligos. Each of these three probes hybridized specifically to a single cucumber chromosome and can be used to paint the homoeologous chromosomes in other Cucumis species that diverged from cucumber for up to 12 million years. The bulked oligo probes can effectively paint and track a single chromosome in early meiotic stages and can be used to map the pairing of a single pair of homoeologous chromosomes in a hybrid between cucumber and its wild relative species Cucumis hystrix.

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黄瓜黑色果刺基因染色体定位及候选基因分析

中国农业科学 2014年第1期47卷 122-132页

作者：刘书林顾兴芳苗晗王烨 yiqun weng Todd C Wehner 张圣平中国农业科学院蔬菜花卉研究所 Department of Horticulture Science North Carolina State University USDA-ARS Vegetable Crops Research Unit Horticulture Department University of Wisconsin

【目的】黄瓜作为重要的果菜类蔬菜,果实品质一直是黄瓜育种研究的重点。果实品质包括内在品质和外观品质,其中外观品质对黄瓜的商品性具有重要影响。果刺颜色作为黄瓜重要的品质性状之一,对其进行遗传分析和基因定位将有助于了解果刺... 详细信息

【目的】黄瓜作为重要的果菜类蔬菜,果实品质一直是黄瓜育种研究的重点。果实品质包括内在品质和外观品质,其中外观品质对黄瓜的商品性具有重要影响。果刺颜色作为黄瓜重要的品质性状之一,对其进行遗传分析和基因定位将有助于了解果刺颜色遗传的分子机理,为黄瓜果实性状改良提供理论依据和技术支撑,同时也可为刺色基因的精细定位及克隆奠定基础。【方法】研究利用黄瓜白色果刺自交系GY14(P1)和黑色果刺自交系NC76(P2)为亲本构建遗传群体,进行黄瓜果刺颜色的遗传分析。以F2分离群体为试材,应用分离群体分组分析法(BSA)和2 112对SSR引物进行SSR分析,结合9 930黄瓜全基因组序列信息和100份核心种质重测序结果开发新标记,对初定位区域进行标记加密。采用JoinMap 4.0作图软件和MapInspect软件构建连锁群并完成连锁群与染色体的对应,实现黄瓜黑色果刺性状的基因定位。运用生物信息学,在定位区域进行候选基因分析。利用包含156个株系的重组自交系(RILs)群体,对黑色果刺基因紧密连锁的两侧翼分子标记进行验证,确定标记用于分子标记辅助选择(MAS)育种的准确性。【结果】研究表明,黄瓜自交系NC76的黑色果刺符合质量性状遗传特点,由显性单基因B控制,黑色对白色为显性。初步定位筛选获得了与B基因连锁的8对SSR引物,将B定位于黄瓜4号染色体(Chr.4)上,最近的连锁标记为SSR22231,遗传距离为10.8 cM;根据初定位区域的序列信息,设计合成了新的SSR引物212对和Indel引物25对,利用这些引物对双亲和F2群体DNA进行分析,最终构建了一个包含14个SSR标记和1个InDel标记的分子标记连锁群,获得与B连锁的两侧翼标记SSRB-181和SSRB-130,遗传距离分别为2.0 cM和1.6 cM,该区段物理距离为422.1 kb,有60个预测候选基因,推测Csa4G003095和Csa4G001690是与黑色果刺形成相关性较大的候选基因。与B紧密连锁的两侧翼标记用于MAS育种的准确率分别为96.8%和96.2%,其中SSRB-181对黑色果刺植株鉴定的准确率达到100%,将在MAS育种发挥重要作用。【结论】黄瓜自交系NC76的黑色果刺性状由显性单基因控制,该基因位于Chr.4上422.1 kb范围内,两侧翼标记为SSRB-181和SSRB-130,遗传距离为3.6 cM。本研究结果为黑色果刺基因的精细定位和克隆及MAS育种奠定了良好基础。

关键词：黄瓜黑色果刺染色体定位 SSR标记候选基因