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Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis

Journal of Integrative Agriculture 2024年第11期23卷 3830-3842页

作者： Dan Chu Bin Chen bo weng Saina Yan Yanfei Yin Xiangwei Tang Maoliang Ran Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Xiangxi Vocational and Technical College for Nationalities Xiangxi 416007China

Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male *** a huge number of long non-coding RNAs(lncRNAs)are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis,their functions and regulatory mechanisms are still in ***,we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli *** results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli *** overexpression promoted cell cycle progression and cell proliferation,as well as inhibited cell apoptosis,whereas siRNA-induced lncFPFSC knockdown resulted in the opposite ***,lncFPFSC acted as a sponge for *** of miR-326 inhibited cell proliferation and induced cell apoptosis,which further abolished the effects of lncFPFSC *** euchromatic histone-lysine N-methyltransferase 2(EHMT2)gene was directly targeted by miR-326,and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli ***,siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 ***,lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 *** study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells,and the competing endogenous RNA(ceRNA)network,and provided new molecular markers to treat Sertoli cell disorder inducing male infertility.

关键词： porcine Sertoli cells proliferation apoptosis lncRNA FPFSC miR-326 EHMT2 gene

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Engineering versatile Au-based catalysts for solar-to-fuel conversion

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Journal of Energy Chemistry 2023年第8期83卷 341-362,I0010页

作者： Chunhua Wang Hongwen Zhang Feili Lai Zhirun Xie Yun Hau Ng bo weng Xuejiao Wu Yuhe Liao Department of Chemistry KU LeuvenCelestijinenlaan 200F3001 LeuvenBelgium cMACS Department of Microbial and Molecular SystemsKU LeuvenCelestijnenlaan 200F3001 LeuvenBelgium School of Energy and Environment City University of Hong Kong83 Tat Chee AvenueKowloonHong Kong 999077China Centre for Sustainable Catalysis and Engineering Faculty of Bioscience EngineeringKU Leuven3001 LeuvenBelgium Guangzhou Institute of Energy Conversion Chinese Academy of SciencesGuangzhou 510640GuangdongChina

Gold(Au) nanostructures(NSs) have been widely employed as cocatalysts to improve the photoactivity of semiconductor materials, while a systematic summary of the engineering approaches of Au NSs to maximize the solar-to-fuel conversion efficiency is still lacking. In this review, the recently developed strategies for elevating the overall photocatalytic performance of Au-based catalysts and the deep physical chemistry mechanisms are highlighted. Firstly, the synthetic approaches of Au NSs are summarized, followed by an elaboration on their multiple functions in improving photoactivity. Afterward, modification strategies of Au NSs used to enhance the photocatalytic efficiency of Au-semiconductor composites,including controlling the Au NSs morphology, size, crystal phase, defect engineering, alloying with different metals, modulating interfacial interaction, and introducing an external field, are summarized and discussed independently. Additionally, advanced characterization techniques that can provide insights into the charge dynamics of the photocatalysts are introduced. Finally, we share our opinion on the challenges and outline potentially promising opportunities and directions for efficient Au-based photocatalysis research moving forward. We sincerely look forward to this review can deliver insightful views to design efficient Au-based photocatalysts and spur certain innovations to other metal-based catalysts.

关键词： Au nanostructures Modification strategies Semiconductor Photocatalysis Solar-to-fuel conversion

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miR-34c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene

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Journal of Integrative Agriculture 2019年第2期18卷 449-459页

作者： RAN Mao-liang weng bo CAO Rong PENG Fu-zhi LUO Hui GAO Hu CHEN Bin College of Animal Science and Technology/Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal Hunan Agricultural UniversityChangsha 410128P.R.China

MicroRNAs(miRNAs) are implicated in swine spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation. Recent studies indicated that miR-34 c is indispensable in the late steps of spermatogenesis. However, whether miR-34 c plays similar important roles in immature porcine Sertoli cells remain unknown. In the present study, we conducted two experiments using a completely randomised design to study the function roles of miR-34 c. The results from experiment I demonstrated that the relative expression level of miR-34 c in swine testicular tissues increased(P=0.0017) quadratically with increasing age, while the relative expression level of SMAD family member 7(SMAD7) decreased(P=0.0009) with curve. Furthermore, miR-34 c expression levels showed a significant negative correlation(P=0.013) with SMAD7 gene expression levels. The results from experiment II indicated that miR-34 c directly targets the SMAD7 gene using a luciferase reporter assay, and suppresses(P<0.05) SMAD7 mRNA and protein expressions in immature porcine Sertoli cells. Overexpression of miR-34 c inhibited(P<0.05) proliferation and enhanced(P<0.05) apoptosis in the immature porcine Sertoli cells, which was supported by the results from the Cell Counting Kit-8(CCK-8) assay, the 5-Ethynyl-2′-deoxyuridine(EdU) assay, and the Annexin V-FITC/PI staining assay. Furthermore, knockdown of SMAD7 via small interfering RNA(siR NA) gave a similar result. It is concluded that miR-34 c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene.

关键词： immature porcine Sertoli cell cell proliferation apoptosis SMAD7 miR-34c

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Visualizing light-induced dynamic structural transformations of Au clusters-based photocatalyst via in situ TEM

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Nano Research 2021年第8期14卷 2805-2809页

作者： bo weng Youhong Jiang Hong-Gang Liao Maarten B.J.Roeffaers Feili Lai Haowei Huang Zichao Tang State Key Laboratory for Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy MaterialsCollege of Chemistry and Chemical Engineering Xiamen UniversityXiamen 361005China cMACS Department of Microbial and Molecular SystemsKU LeuvenCelestijnenlaan 200F3001 LeuvenBelgium Department of Chemistry KU LeuvenCelestijnenlaan 200F3001 LeuvenBelgium

Ultrasmall gold(Au)clusters have been regarded as one of the prototypes materials for solar energy conversion due to their unique strong molecular-like light absorption ***,the light-induced aggregation of Au clusters into nanoparticles is one of the most important factors that restricts its application in *** Au clusters aggregation has been widely demonstrated,the underlying mechanism for cluster fusion is still unclear due to the lack of experimental ***,we report the direct observation of Au clusters on TiO_(2) nanosheets aggregating when used as visible light photocatalysts for the reduction of *** in situ high-resolution transmission electron microscopy(TEM),the coexistence of two fusion mechanisms of Au clusters on TiO_(2) under ultraviolet-visible(UV-Vis)light irradiation in air is identified,i.e.,the migration and coalescence(MC)and Ostwald ripening(OR).Additionally,the correlation between the photostability of Au clusters and reaction atmospheres has been investigated,among which Au clusters have higher stability in an inert N_(2) atmosphere or vacuum than the oxidizing atmospheres(i.e.,air and O_(2)).These results indicate the inherent stability of Au cluster during photocatalysis,and instability comes from the consuming of ligand *** work not only discloses the underlying mechanism of Au cluster sintering but also provides guidelines for enhancing metal clusters-based photocatalysts stability.

关键词： photocatalysis dynamic structural transformation Au clusters in situ TEM

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Taxus chinensis ameliorates diabetic nephropathy through down-regulating TGF-β1/Smad pathway

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Chinese Journal of Natural Medicines 2018年第2期16卷 90-96页

作者： weng Hong-bo HAN Wen-Ke XIONG Yan-Wen JIN Zhou-Hui LAN Zhen LIU Cheng ZHANG Xue-Mei PENG Wen Department of Pharmacology School of Pharmacy Fudan University Department of Nephrology Putuo Hospital Shanghai University of Traditional Chinese Medicine Liqun Hospital Shanghai

Diabetic nephropathy(DN) is one of the common microvascular complications of diabetes mellitus. Renal fibrosis is closely related to the deterioration of renal function. The present study aimed to investigate protective effect of Taxus chinensis on high-fat diet/streptozotocin-induced DN in rats and explore the underlying mechanism of action. The rat DN model was established via feeding high fat diet for 4 weeks and subsequently injecting streptozotocin(30 mg·kg^(-1) body weight) intraperitoneally. The rats with blood glucose levels higher than 16.8 mmol·L^(-1) were selected for experiments. The DN rats were treated with Taxus chinensis orally(0.32, 0.64, and 1.28 g·kg^(-1)) once a day for 8 weeks. Taxus chinensis significantly improved the renal damage, which was indicated by the decreases in 24-h urinary albumin excretion rate, blood serum creatinine, and blood urea nitrogen. Histopathological examination confirmed the protective effect of Taxus chinensis. The thickness of glomerular basement membrane was reduced, and proliferation of mesangial cells and podocytes cells and increase in mesangial matrix were attenuated. Further experiments showed that Taxus chinensis treatment down-regulated the expression of TGF-β1 and α-SMA, inhibited phosphorylation of Smad2 and Smad3. These results demonstrated that Taxus chinensis alleviated renal injuries in DN rats, which may be associated with suppressing TGF-β1/Smad signaling pathway.

关键词： Diabetic nephropathy Taxus chinensis TGF-βl/Smad signaling pathway

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miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene

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Journal of Integrative Agriculture 2019年第8期18卷 1924-1935页

作者： weng bo RAN Mao-liang CAo Rong PENG Fu-zhi LUo Hui GAo Hu TANG Xiang-wei YANG An-qi CHEN Bin College of Animal Science and Technology/Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal Hunan Agricultural University

MicroRNAs(miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically(Pboth regulated(P0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted(P<0.05) porcine immature Sertoli cell proliferation by targeting DAZAP1 gene. Overall, these experiments demonstrated that miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene.

关键词： miR-10b DAZAP1 gene expression proliferation porcine immature Sertoli cell

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Houttuynia cordata polysaccharide alleviated intestinal injury and modulated intestinal microbiota in H1N1 virus infected mice

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Chinese Journal of Natural Medicines 2019年第3期17卷 187-197页

作者： CHEN Mei-Yu LI Hong LU Xiao-Xiao LING Li-Jun weng Hong-bo SUN Wei CHEN Dao-Feng ZHANG Yun-Yi Department of Pharmacology School of Pharmacy Fudan University Department of Pharmacognosy School of Pharmacy Fudan University

Houttuynia cordata polysaccharide(HCP) is extracted from Houttuynia cordata, a key traditional Chinese medicine. The study was to investigate the effects of HCP on intestinal barrier and microbiota in H1N1 virus infected mice. Mice were infected with H1N1 virus and orally administrated HCP at a dosage of 40 mg×kg^(–1)×d^(–1). H1N1 infection caused pulmonary and intestinal injury and gut microbiota imbalance. HCP significantly suppressed the expression of hypoxia inducible factor-1α and decreased mucosubstances in goblet cells, but restored the level of zonula occludens-1 in intestine. HCP also reversed the composition change of intestinal microbiota caused by H1N1 infection, with significantly reduced relative abundances of Vibrio and Bacillus, the pathogenic bacterial genera. Furthermore, HCP rebalanced the gut microbiota and restored the intestinal homeostasis to some degree. The inhibition of inflammation was associated with the reduced level of Toll-like receptors and interleukin-1β in intestine, as well as the increased production of interleukin-10. Oral administration of HCP alleviated lung injury and intestinal dysfunction caused by H1N1 infection. HCP may gain systemic treatment by local acting on intestine and microbiota. This study proved the high-value application of HCP.

关键词： H1N1 influenza virus Houttuynia cordata Inflammation Intestinal Barrier Microbiota Polysaccharide

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我国准市场模式的溃败与政府责任缺失

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东北大学学报（社会科学版） 2010年第2期12卷 135-140页

作者：翁博北京大学政府管理学院北京100871

日益成为全球政府治理主导范式的准市场模式在中国遭遇了溃败,正承受着批评、质疑甚至否定。立足于对我国多个领域民营化改革实践的分析,并结合相关理论提出和论证的核心命题是:我国民营化实践中出现的矛盾和问题不能简单归咎于准市场... 详细信息

日益成为全球政府治理主导范式的准市场模式在中国遭遇了溃败,正承受着批评、质疑甚至否定。立足于对我国多个领域民营化改革实践的分析,并结合相关理论提出和论证的核心命题是:我国民营化实践中出现的矛盾和问题不能简单归咎于准市场模式本身,症结在于转型时期政府责任的严重缺失。主要表现为:财政投入严重不足,缺乏必要的监管和调控,未能进行有效的管理并充当精明买主。当政府责任回归之后,我国准市场模式新的变革时代将重新来临。

关键词：政府治理准市场模式市场化民营化政府责任缺失

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Innovation in neglected tropical disease drug discovery and development

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Infectious Diseases of Poverty 2018年第1期7卷 654-662页

作者： Hong-bo weng Hai-Xia Chen Ming-Wei Wang School of Pharmacy Fudan University826 Zhangheng RoadPudong New DistrictShanghai 201203China The National Center for Drug Screening and the CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of Sciences(CAS)189 Guoshoujing RoadPudong New DistrictShanghai 201203China School of Life Science and Technology ShanghaiTech University393 Middle Huaxia RoadPudong New DistrictShanghai 201210China

Background:Neglected tropical diseases(NTDs)are closely related to poverty and affect over a billion people in developing *** unmet treatment needs cause high mortality and disability thereby imposing a huge burden with severe social and economic *** coordinated by the World Health Organization,various philanthropic organizations,national governments and the pharmaceutical industry have been making efforts in improving the situation,the control of NTDs is still inadequate and extremely difficult *** lack of safe,effective and affordable medicines is a key contributing *** paper reviews the recent advances and some of the challenges that we are facing in the fight against *** body:In recent years,a number of innovations have demonstrated propensity to promote drug discovery and development for *** of multilateral collaborations leads to continued efforts and plays a crucial role in drug *** approaches and advanced technologies are urgently needed in drug innovation for ***,the control and elimination of NTDs remain a formidable task as it requires persistent international cooperation to make sustainable progresses for a long period of *** currently employed strategies were proposed and verified to be successful,which involve both mechanisms of‘Push’which aims at cutting the cost of research and development for industry and‘Pull’which aims at increasing market *** to this effort should be the exercise of shared responsibility globally to reduce risks,overcome obstacles and maximize *** NTDs are closely associated with poverty,it is absolutely essential that the stakeholders take concerted and long-term measures to meet multifaceted challenges by alleviating extreme poverty,strengthening social intervention,adapting climate changes,providing effective monitoring and ensuring timely ***:The ongoing endeavor at the global scale wil

关键词： Innovation Collaboration Open-source Neglected tropical diseases

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Sustainable Agricultural Model of High Output, Low Input and Less Pollution in Paddy Field

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Agricultural Sciences in China 2001年第1期 123-129页

作者： TANG Long-fei HUANG Yi-bin weng bo-qi LIU Zhong-zhu LIU Xia-shi National Azolla Research Center Fujian Academy of Agricultural Sciences Fuzhou 350013 National Azolla Research Center Fujian Academy of Agricultural Sciences Fuzhou 350013 National Azolla Research Center Fujian Academy of Agricultural Sciences Fuzhou 350013 National Azolla Research Center Fujian Academy of Agricultural Sciences Fuzhou 350013 National Azolla Research Center Fujian Academy of Agricultural Sciences Fuzhou 350013

A model of sustainable agriculture of "high output, low input and less pollution" (HLL model)in paddy (Oryza L. )fields has been developed. About 10 - 13t/ha of rice and 3 - 4t/ha of fish were harvested by adopting the comprehensive technical system. The amount of chemical fertilizer and pesticide decreased by about 50%, thus greatly controlled the environmental pollution, and simultaneously decreased the methane emission. It was concluded that these comprehensive techniques for this model should be popularized.

关键词： Rice-azolla- fish CH4 emission Potassium enrichment HLL model

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