miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene

作     者：WENG Bo RAN Mao-liang CAo Rong PENG Fu-zhi LUo Hui GAo Hu TANG Xiang-wei YANG An-qi CHEN Bin 

作者机构：College of Animal Science and Technology/Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal Hunan Agricultural University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2019年第18卷第8期

页      面：1924-1935页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：financially supported by the earmarked fund for China Agriculture Research System (CARS-36) the Hunan Provincial Natural Science Foundation of China (2018JJ2176 and 2018JJ3219) 

主　　题：miR-10b DAZAP1 gene expression proliferation porcine immature Sertoli cell 

摘      要：MicroRNAs(miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically(P0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted(P0.05) porcine immature Sertoli cell proliferation by targeting DAZAP1 gene. Overall, these experiments demonstrated that miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene.