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Annexin A2 silencing inhibits invasion, migration, and tumorigenic potential of hepatoma cells

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World Journal of Gastroenterology 2013年第24期19卷 3792-3801页

作者： Hai-Jian Zhang Deng-Fu Yao Min Yao Hua Huang Li Wang Mei-Juan yan Xiao-Di yan Xing Gu wei Wu Shao-Lin Lu Research Centre of Clinical Medicine Affiliated Hospital of Nantong University Department of Pathology Affiliated Hospital of Nantong University Department of Clinical Oncology Affili-ated Hospital of Nantong University

AIM: To investigate the effects of Annexin A2 (ANXA2) silencing on invasion, migration, and tumorigenic potential of hepatoma cells. METHODS: Human hepatoma cell lines [HepG2, SMMC-7721, SMMC-7402, and MHCC97-H, a novel human hepatocellular carcinoma (HCC) cell line with high metastasis potential] and a normal hepatocyte cell line(LO2) were used in this study. The protein and mRna expression levels of ANXA2 were analysed by western blotting and real-time polymerase chain reaction, re-spectively. The intracellular distribution profile of ANXA2 expression was determined by immunofluorescence and immunohistochemistry. Short hairpin Rna target-ing ANXA2 was designed and stably transfected into MHCC97-H cells. Cells were cultured for in vitro analy-ses or subcutaneously injected as xenografts in mice for in vivo analyses. Effects of ANXA2 silencing on cell growth were assessed by cell counting kit-8 (CCK-8) as-say (in vitro ) and tumour-growth assay (in vivo ), on cell cycling was assessed by flow cytometry and propidium iodide staining (in vitro ), and on invasion and migration potential were assessed by transwell assay and wound-healing assay, respectively (both in vitro ). RESULTS: The MHCC97-H cells, which are known to have high metastasis potential, showed the highest lev-el of ANXA2 expression among the four HCC cell types examined; compared to the LO2 cells, the MHCC97-H expression level was 8-times higher. The ANXA2 expres-sion was effectively inhibited (about 80%) by ANXA2-specific small hairpin Rna (shRna). ANXA2 expression in the MHCC97-H cells was mainly localized to the cel-lular membrane and cytoplasm, and some localization was detected in the nucleus. Moreover, the proliferation of MHCC97-H cells was obviously suppressed by shR-na-mediated ANXA2 silencing in vitro , and the tumour growth inhibition rate was 38.24% in vivo . The per-centage of MHCC97-H cells in S phase dramatically de-creased (to 27.76%) under ANXA2-silenced conditions. Furthermore, ANXA2-s

关键词： Annexin A2 Small hairpin Rna Hepatocel-lular carcinoma Invasion Migration Tumorigenic po-tential

Advanced Space-based Solar Observatory(ASO-S):an overview

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Research in Astronomy and Astrophysics 2019年第11期19卷 3-10页

作者： wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua yan Jun Lin wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li wei Chen You-Ping Li Ying-na Su Hai-yan Wu Mei Gu Lei Huang Xue-Jun Tang Key Laboratory of Dark Matter and Space Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210034China Innovation Academy for Microsatellites Chinese Academy of SciencesShanghai 201203China national Astronomical Observatories Chinese Academy of SciencesBeijing 100101China nanjing Institute of Astronomical Optics&Technology National Astronomical ObservatoriesChinese Academy of SciencesNanjing 210042China Changchun Institute of Optics Fine Mechanics and PhysicsChangchun 130033China Xi’an Institute of Optics and Precision Mechanics Chinese Academy of SciencesXi’an 710119China national Space Science Center Chinese Academy of SciencesBeijing 100190China School of Astronomy and Space Science Nanjing UniversityNanjing 210023China Yunnan Observatories Chinese Academy of SciencesKunming 650011China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 101408China Beijing Special Engineering and Design Institute Beijing 100028China Beijing Institute of Tracking and Telecommunications Technology Beijing 100094China Shanghai Academy of Spaceflight Technology Shanghai 201109China

The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar *** scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single ***-S is scheduled to be launched into a 720 km Sun-synchronous orbit in *** paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C.

关键词： space vehicles instruments-Sun magnetic fields-Sun flares-Sun CMEs-Sun UV radiation-Sun X-ray,gamma-rays

Starch Synthesis and Programmed Cell Death during Endosperm Development in Triticale(× Triticosecale Wittmack)

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Journal of Integrative Plant Biology 2010年第7期52卷 602-615页

作者： Chun-yan Li wei-Hua Li Cheng Li Denis A. Gaudet Andrd Laroche Lian-Pu Cao Zhen-Xiang Lu College of Agriculture Shihezi University Xinjiang 832003 China Lethbridge Research Centre Agriculture and Agri-Food Canada Alberta T1J 4B1 Canada

Triticale （x TriticosecaleWittmack） grains synthesize and accumulate starch as their main energy source. Starch accumulation rate and synthesis activities of ADP-glucose pyrophosphorylase, soluble starch synthases, granule-bound starch synthase and starch-branching enzyme showed similar pattern of unimodal curves during endosperm development. There was no significant difference in activity of the starch granule-bound protein isolated from total and separated starch granules at different developmental stages after anthesis in triticale. Evans Blue staining and analysis of Dna fragmentation indicated that cells of triticale endosperm undergo programmed cell death during its development. Dead cells within the endosperm were detected at 6 d post anthesis （DPA）, and evidence of Dna fragmentation was first observed at 21 DPA. The period between initial detection of PCD to its rapid increase overlapped with the key stages of rapid starch accumulation during endosperm development. Cell death occurred stochastically throughout the whole endosperm, meanwhile, the activities of starch biosynthetic enzymes and the starch accumulation rate decreased in the late stages of grain filling. These results suggested that the timing and progression of PCD in triticale endosperm may interfere with starch synthesis and accumulation.

关键词： Triticosecale Triticales (product) Starch Grain Rations Restricted feeding Endosperm Death Dna Flowering

Protocol of Jidong Women Health Cohort Study: Rationale, Design, and Baseline Characteristics

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Biomedical and Environmental Sciences 2019年第2期32卷 144-152页

作者： QIU Meng Si WANG Xian wei YAO yan GE Si Qi LIU Hua Min DAI Ying Chun ZHOU Yong Department of Epidemiology School of Public Health Southern Medical University (Guangdong Provincial Key Laboratory of Tropical Disease Research) Sanbo Brain Institute Sanbo Brain Hospital Capital Medical University Department of Cardiology Beijing Anzhen Hospital Capital Medical University Department of Neuroepidemiology Beijing Neurosurgical Institute Capital Medical University School of Public Health Taishan Medical University

Objective The Jidong Women Health Cohort Study is a prospective cohort study on female-specific characteristics and risks of chronic diseases in Chinese women and focuses on the potential association between menopause and risks of cardiovascular disease(CVD). Methods The study includes 4,179 female participants with an age of older than 18 years from Caofeidian district, Tangshan city, northern China. Baseline information on female-specific characteristics and potential cardiovascular risk factors was collected and all the participants underwent a physical examination with blood samples collected in 2013. To establish a better risk assessment tool of female CVD, updated information from questionnaire investigation, physical examinations and occurrence of outcome events will be collected through a longitudinal follow-up annually up to the year 2024. Results At baseline, Mean age of the participants was 42.3 ± 12.8 years. Reproduction occurred in 2,948 participants(70.5%), menopausal transition in 173(4.3%), and postmenopause in 1,058(25.3%). The incidence of arterial hypertension, dyslipidemia, and diabetes showed significant difference across different groups stratified by Stage of Reproductive Aging Workshop(STRAW) system(P < 0.05). Conclusion The Jidong Women Health Cohort Study will contribute to the scientific evidence on association between female-specific characteristics and cardiovascular risks, and will also be helpful to provide a new path for early detection and prevention of CVD.

关键词： Cardiovascular disease Menopause Women Protocol Chinese population-based

Heart rate-adjusted PR as a prognostic marker of long-term ventricular arrhythmias and cardiac death in ICD/CRT-D recipients

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Journal of Geriatric Cardiology 2019年第3期16卷 259-264页

作者： Yu-Qiu LI Shuang ZHAO Ke-Ping CHEN yang-Gang SU wei HUA Si-Lin CHEN Zhao-Guang LIANG wei XU yan DAI Xiao-Han FAN Shu ZHANG State Key Laboratory of Cardiovascular Disease Arrhythmia CenterFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Department of Cardiology Shanghai Institute of Cardiovascular DiseasesZhongshan HospitalFudan UniversityShanghaiChina Department of Cardiology Guangdong Cardiovascular InstituteGuangdong General HospitalGuangzhouChina Department of Cardiology First Affiliated Hospital of Harbin Medical UniversityHarbinChina Department of Cardiology Nanjing Drum Tower HospitalNanjingChina

Objective To evaluate the PR to RR interval ratio (PR/RR,heart rate-adjusted PR) as a prognostic marker for long-term ventricular arrhythmias and cardiac death in patients with implantable cardioverter defibrillator (ICDs) and cardiac resynchronization therapy with defibrillators (CRT-D).Methods We retrospectively analyzed data from 428 patients who had an ICD/CRT-D equipped with home *** PR and RR interval data prior to ICD/CRT-D implantation were collected from standard 12-lead electrocardiograph,and the PR/RR was *** primary endpoint was appropriate ICD/CRT-D treatment of ventricular arrhythmias (VAs),and the secondary endpoint was cardiac *** During a mean follow-up period of 38.8 ± 10.6 months,197 patients (46%) experienced VAs,and 47 patients (11%) experienced cardiac *** overall PR interval was 160 ± 40 ms,and the RR interval was 866 ± 124 *** on the receiver operating characteristic curve,a cut-off value of 18.5% for the PR/RR was identified to predict VAs.A PR/RR ≥ 18.5% was associated with an increased risk of VAs [hazard ratio (HR)= 2.243,95% confidence interval (CI)= 1.665–3.022,P nadjusted *** adjustment in a multivariate Cox model,the relationship remained significant among PR/RR ≥ 18.5%,VAs (HR = 2.230,95%CI = 1.555–2.825,P < 0.001) and cardiac death (HR = 2.105,95%CI = 1.101–4.025,P = *** A PR/RR ≥ 18.5% at baseline can serve as a predictor of future VAs and cardiac death in ICD/CRT-D recipients.

关键词： Implantable cardioverter defibrillator PR interval RR interval Ventricular arrhythmias

Extraction and biodegradation of ginkgolic acids from Ginkgo biloba sarcotestae

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Frontiers of Agricultural Science and Engineering 2017年第4期4卷 465-472页

作者： Qi LI wei SUN yan JIANG Fuliang CAO Guibin WANG Linguo ZHAO Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University College of Chemical Engineering Nanjing Forestry University College of Forestry Nanjing Forestry University

Ginkgolic acids are unwanted constituents in standard Ginkgo biloba leaves extracts. Thus, for the quality control of ginkgo extracts, it is important to establish an effective degradation method, with high catalytic efficiency and safety, to remove ginkgolic *** are oxidases with potential for application in elimination of hazardous phenolic compounds. In this study, single-factor and orthogonal experiments were used to optimize extraction of ginkgolic acid from G. biloba sarcotestae. The results showed that ethanol was the best solvent, with the highest extraction rate for ginkgolic acid at 85% ethanol. On this basis, we measured ethanol volume fraction, extraction time, temperature and solidliquid ratio using an orthogonal experiment. By using absorbance of 310 nm as standard, the optimal extraction conditions were 85% ethanol with, solid-liquid ratio of1:14 at 40°C for 12 h. These conditions gave a ginkgolic acid yield of 73.1 mg·g^(–1). Subsequently, recombinant laccase was used to degrade the ginkgolic acid in several laccase/mediator systems, of which Lac C was the best. At50°C, p H 4.5, enzyme concentration of 0.01 U·m L^(–1),0.5 mmol·L^(–1) mediator ABTS and reaction time of 3 h, the degradation rate of ginkgolic acid reached 100%. These results lay the foundation for research on and application of biological enzymes for detoxification of G. biloba extracts.

关键词： biodegradation extraction ginkgolic acid laccase orthogonal method

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在滞尘影响下的植被叶片光谱变化特征研究

光谱学与光谱分析 2013年第10期33卷 2715-2720页

作者：罗娜娜赵文吉晏星首都师范大学三维信息获取与应用教育部重点实验室首都师范大学资源环境与地理信息系统北京市重点实验室香港理工大学土地测量及地理资讯学系

为建立以高光谱数据为基础的叶片滞尘质量反演模型,沿北京市区采集了30个大叶黄杨叶片样本。利用电子分析天平和光谱仪(analytical spectral devices ASD FieldSpec Pro)测定"除尘前"与"除尘后"叶片质量及光谱反射率曲线,以获取叶片尘... 详细信息

为建立以高光谱数据为基础的叶片滞尘质量反演模型,沿北京市区采集了30个大叶黄杨叶片样本。利用电子分析天平和光谱仪(analytical spectral devices ASD FieldSpec Pro)测定"除尘前"与"除尘后"叶片质量及光谱反射率曲线,以获取叶片尘埃量、光谱信息等数据。随后以传统意义和偏最小二乘(PLS)回归模型为基础,以探究空气尘埃量与光谱曲线之间可能存在的关系,阐述了叶片尺度上尘埃量对植物光谱特征的影响。结果为:除尘前后叶片光谱曲线在350～700,780～1 300,1 900～2 500nm波段区间内有较大差异,同时尘埃量与叶片单波段光谱反射率比值呈负相关,相关度最大值点为737波段,属于近红外波段,相关系数可达-0.8左右。在尘埃量与叶片光谱多波段组合关系研究中得到,948和945波段构成的NDVI指数与尘埃量的相关度最大,相关系数可达0.76。在叶片滞尘量反演研究中,对比传统意义滞尘量回归模型,引进的偏最小二乘算法(PLS)可使叶片滞尘量反演精度略有提高,最后由回归模型精度评定可得偏最小二乘法反演效果较传统回归好。

关键词：大叶黄杨高光谱尘埃量反演回归分析偏最小二乘法

Aggravated inflammation and increased expression of cysteinyl leuko-triene receptors in the brain after focal cerebral ischemia in AQP4-deficient mice

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Neuroscience Bulletin 2012年第6期28卷 680-692页

作者： Wen-Zhen Shi Chun-Zhen Zhao Bing Zhao Qiao-Juan Shi Li-Hui Zhang yan-Fang Wang San-Hua Fang Yun-Bi Lu wei-Ping Zhang Er-Qing wei Institute of Neuroscience and Department of Pharmacology School of MedicineZhejiang University Hangzhou Key Laboratory of Neurobiology and Department of Pharmacology School of Basic MedicineHangzhou Normal University

Objective Aquaporin-4 （AQP4）, the main water channel protein in the brain, plays a critical role in water homeostasis and brain edema. Here, we investigated its role in the inflammatory responses after focal cerebral ischemia. Methods In AQP4-knockout （KO） and wild-type mice, focal cerebral ischemia was induced by 30 rain of middle cerebral arterial occlusion （MCAO）. Ischemic neuronal injury and cellular inflammatory responses, as well as the expression and localization of cysteinyl leukotriene CysLT2 and CysLT~ receptors, were determined at 24 and 72 h after MCAO. Results AQP4-KO mice showed more neuronal loss, more severe microglial activation and neutrophil infiltration, but less astrocyte proliferation in the brain after MCAO than wild-type mice. In addition, the protein levels of both CysLT1 and CysLT2 receptors were up-regulated in the ischemic brain, and the up-regulation was more pronounced in AQP4-KO mice. The CysLT1 and CysLT2 receptors were primarily localized in neurons, microglia and neutrophils; those localized in microglia and neutrophils were enhanced in AQP4-KO mice. Conclusion AQP4 may play an inhibitory role in postischemic inflammation.

关键词： aquaporin 4 gene deficiency inflammation cysteinyl leukotriene receptor microglia astrocyte focal cerebral ischemia

Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome

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Biomedical and Environmental Sciences 2019年第5期32卷 324-333页

作者： JIANG Han Ji CHEN na SHEN Zhi Qiang YIN Jing QIU Zhi Gang MIAO Jing yanG Zhong wei SHI Dan yang WANG Hua Ran WANG Xin wei LI Jun Wen yanG Dong JIN Min Department of Environment and Health Tianjin Institute of Environmental & Operational Medicine Key Laboratory of Risk Assessment and Control for Environment & Food Safety

Objective To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1(PV1). Methods We used cell culture, long‐overlapping RT‐PCR, and spot hybridization assays to verify and accurately locate the sites of action of ozone that cause PV1 inactivation. We also employed recombinant viral genome Rna infection models to confirm our observations. Results Our results indicated that ozone inactivated PV1 primarily by disrupting the 5′-non‐coding region(5′‐NCR) of the PV1 genome. Further study revealed that ozone specifically damaged the 80‐124 nucleotide(nt) region in the 5′-NCR. Recombinant viral genome Rna infection models confirmed that PV1 lacking this region was non‐infectious. Conclusion In this study, we not only elucidated the mechanisms by which ozone induces PV1 inactivation but also determined that the 80‐124 nt region in the 5′‐NCR is targeted by ozone to achieve this inactivation.

关键词： Poliovirus Poliomyelitis Ozone Disinfection