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Apical meristem transcriptome analysis identifies a role for the blue light receptor gene GhFKF1 in cotton architecture development

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The Crop Journal 2024年第4期12卷 1126-1136页

作者： Xiao Li Yuanlong Wu Zhenping Liu hengling wei Hantao Wang Shuxun Yu National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070HubeiChina State Key Laboratory of Cotton Biology Institute of Cotton Research of the Chinese Academy of Agricultural SciencesAnyang 455000HenanChina State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityLin’an 311300ZhejiangChina Xianghu Laboratory Hangzhou 311200ZhejiangChina

Cotton architecture is determined by the differentiation fate transition of axillary meristem(AM),and influences cotton yield and the efficiency of mechanized *** observed that the initiation of flowering primordium was earlier in early-maturing than that in late-maturing cultivars during the differentiation and development of *** RNA-Seq and expression level analyses showed that genes FLAVIN BINDING,KELCH REPEAT,F-BOX1(GhFKF1),and GIGANTEA(GhGI)were in response to circadian rhythms,and involved in the regulation of cotton *** gene structure,predicted protein structure,and motif content analyses showed that in Arabidopsis,cotton,rapseed,and soybean,proteins GhFKF1 and GhGI were functionally conserved and share evolutionary *** to the wild type,in GhFKF1 mutants that were created by the CRISPR/Cas9 system,the initiation of branch primordium was ***,the knocking out of GhGI increased the number of AM differentiating into flower primordium,and there were much more lateral branch differentiation and ***,we investigated that proteins GhFKF1 and GhGI can interact with each *** results suggest that GhFKF1 and GhGI are key regulators of cotton architecture development,and may collaborate to regulate the differentiation fate transition of AM,ultimately influencing plant *** describe a strategy for using the CRISPR/Cas9 system to increase cotton adaptation and productivity by optimizing plant architecture.

关键词： Cotton Axillary meristem GhFKF1 GhGI Plant architecture

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Molecular Cloning and Function Analysis of Two SQUAMOSA-Like MADS-Box Genes From Gossypium hirsutum L.

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Journal of Integrative Plant Biology 2013年第7期55卷 597-607页

作者： Wenxiang Zhang Shuli Fan Chaoyou Pang hengling wei Jianhui Ma Meizhen Song Shuxun Yu State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agriculture Sciences College of Agronomy Northwest A & F University

The MADS-box genes encode a large family of transcription factors having diverse roles in plant development. The SQUAMOSA （SQUA）/APETALA1 （AP1）/FRUITFULL （FUL） subfamily genes are essential regulators of floral transition and floral organ identity. Here we cloned two MADS-box genes, GhMADS22 and GhMADS23, belonging to the SQUA/AP1/FUL subgroup from Gossypium hirsutum L. Phylogenetic analysis and sequence alignment showed that GhMAD$22 and GhMADS23 belonged to the euFUL and euAP1 subclades, respectively. The two genes both had eight exons and seven introns from the start codon to the stop codon according to the alignment between the obtained cDNA sequence and the Gossypium raimondii L. genome sequence. Expression profile analysis showed that GhMADS22 and GhMADS23 were highly expressed in developing shoot apices, bracts, and sepals. Gibberellic acid promoted GhMADS22 and GhMADS23 expression in the shoot apex. Transgenic Arabidopsis lines overexpressing 35S：：GhMADS22 had abnormal flowers and bolted earlier than wild type under long-day conditions （16 h light/8 h dark）. Moreover, GhMADS22 over- expression delayed floral organ senescence and abscission and it could also respond to abscisic acid. In summary, GhMADS22 may have functions in promoting flowering, improving resistance and delaying senescence for cotton and thus it may be a candidate target for promoting early-maturation in cotton breeding.

关键词： Arabidopsis transformation flowering time MADS-box SQUAMOSA-like overexpression.

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Selection and Characterization of a Novel Photoperiod-Sensitive Male Sterile Line in Upland Cotton

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Journal of Integrative Plant Biology 2013年第7期55卷 608-618页

作者： Jianhui Ma hengling wei Ji Liu Meizhen Song Chaoyou Pang Long Wang Wenxiang Zhang Shuli Fan Shuxun Yu State Key Laboratory of Cotton Biology the Institute of Cotton Researchthe Chinese Academy of Agricultural Sciences College of Life Sciences Henan Normal University College of Agronomy Northwest A & F University

Upland cotton （Gossypium hirsutum L.） shows strong heterosis. However, heterosis is not widely utilized owing to the high cost of hybrid seed production. Creation of a photoperiod-sensitive genetic male sterile line could substantially reduce the cost of hybrid seed production in upland cotton. Such a mutant with virescent marker was found by space mutation in near-earth orbit and its traits had been stable after 4 years of selection in Anyang and Sanya, China. This mutant was fertile with an 11-12.5 h photoperiod when the temperature was higher than 21.5 ℃ and was sterile with a 13-14.5 h photoperiod. Genetic analysis indicated that both traits were controlled by a single recessive gene or two closely linked genes. Also, the cytological observations and transcriptome profiling analysis showed that the degradation of pollen grain cytoplasm should be the primary reason why the mutant line were male sterile under long-day conditions.

关键词： Photoperiod-sensitive genetic male sterile sterility mechanism upland cotton virescent marker.

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Genome wide identification and characterization of MAPK genes reveals their potential in enhancing drought and salt stress tolerance in Gossypium hirsutum

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Journal of Cotton Research 2022年第3期5卷 261-273页

作者： SADAU Salisu Bello MEHARI Teame Gereziher AHMAD Adeel TAJO Sani Muhammad IBRAHIM Sani IQBAL Muhammad Shahid ELASAD Mohammed ZHANG Jingjing wei hengling YU Shuxun State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of AgricultureOil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062China Department of Science and Technology Education Bayero University KanoP.O.Box 3011KanoNigeria Agricultural Research Corporation(ARC) P.O.Box 126Wad MedaniSudan.

Background:The cotton crop is universally considered as protein and edible oil source besides the major contributor of natural fiber and is grown in tropical and subtropical regions around the world Unpredicted environmental stresses are becoming significant threats to sustainable cotton production,ultimately leading to a substantial irreversible economic ***-activated protein kinase(MAPK)is generally considered essential for recognizing environmental stresses through phosphorylating downstream signal pathways and plays a vital role in numerous biological ***:We have identified 74 MAPK genes across cotton,41 from ***,19 from ***,whereas 14 have been identified from *** MAPK gene-proteins have been further studied to determine their physicochemical characteristics and other essential *** this perspective,characterization,phylogenetic relationship,chromosomal mapping,gene motif,cis-regulatory element,and subcellular localization were carried *** on phylogenetic analysis,the MAPK family in cotton is usually categorized as A,B,C,D,and E *** to the results of the phylogenic relationship,cotton has more MAPKS genes in Clade A than Clade *** cis-elements identified were classified into five groups(hormone responsiveness,light responsiveness,stress responsiveness,cellular development,and binding site).The prevalence of such elements across the promoter region of these genes signifies their role in the growth and development of *** GHMAPK genes(GH_A07G1527,GH_D02G1138,GH_D03G0121,GH_D03G1517,GH_D05G1003,GH_D11G0040,and GH_D12G2528)were selected,and specific tissue expression and profiling were performed across drought and salt *** expressed that six genes were upregulated under drought treatment except for GH_D11G0040 which is *** all the seven genes have been upregulated at various hours of salt stress ***:RNA sequence and qPCR results showe

关键词： Cotton MAPK gene Drought stress Salt stress

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Affects on Agronomy Traits of Different Mature Cotton Varieties（Gossypium hirsutum L.） by Late Sowing

Affects on Agronomy Traits of Different Mature Cotton Variet...

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中国农学会棉花分会2017年年会暨第九次会员代表大会

作者： Song Meizhen Yu Shuxun Fan Shuli Pang Chaoyou wei hengling State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Because of higher labor costs and comparative effectiveness decline in China,cotton planting areas have been declined ***,barley,rape and cotton double cropping system will be key cotton planting types in Yellow River and Yangtze River cotton planting *** the growing season later may allow the crop to develop under more bloom favorable weather patterns and escape late season low *** objectives were to determine the affects of late planting on agronomy traits of different mature cotton *** data of plant growth,earliness,yield and fiber quality as agronomy traits were collected from five genotypes planted at April 25（early sowing） and May 25（late sowing） in 2012 and2013 *** results showed that plant height,branch numbers and boll numbers were significantly affected by cultivars and planting *** of the first square was significantly shorten and harvested rate before frost（HRBF） was significantly decreased for five different mature cotton cultivars in late *** HRBF was 29.9%for Lumianyan 28,18.8%for CCRI 41 and 25.8%for CCRI 45 were significantly lower than that of 78.3%for CCRI 50 and 81.0%for CCRI 74 in late *** of the first flower,date of the first open boll of Lumianyan 28,CCRI 41 and CCRI 45 were significantly prolonged compared with that of CCRI 50 and CCRI 74 in late *** of node first sympodial branch of Lumianyan 28,CCRI 41 and CCRI 45 was significantly higher than that of CCRI 50 and CCRI 74 in late *** and yield-related traits were significantly decreased for spring cotton varieties,Lumianyan 28,CCRI 41 and CCRI 45,but for summer cotton varieties,CCRI 50 and CCRI 74 Yield and yield-related traits were less affected by late *** quality was less affected for five cotton *** length,fiber strength and uniformity index were not significantly affected by late *** elongation and yellowness were significantly increased in late ***,in doubl

关键词： cotton growth earliness yield fiber quality late sowing

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棉花早熟性研究进展及其应用

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棉花学报 2017年第B09期29卷 1-10页

作者：喻树迅王寒涛魏恒玲宿俊吉中国农业科学院棉花研究所/棉花生物学国家重点实验室河南安阳455000

早熟棉适于麦(油)后直播,实现粮棉一年两熟,对棉花产业稳定发展具有重要意义。本文对早熟性的遗传特性、早熟相关QTL定位、早熟相关基因挖掘的进展进行了阐述;总结了早熟棉的发展与育种现状以及早熟棉种质资源的创新利用;介绍了早熟棉... 详细信息

早熟棉适于麦(油)后直播,实现粮棉一年两熟,对棉花产业稳定发展具有重要意义。本文对早熟性的遗传特性、早熟相关QTL定位、早熟相关基因挖掘的进展进行了阐述;总结了早熟棉的发展与育种现状以及早熟棉种质资源的创新利用;介绍了早熟棉在我国黄河流域、长江流域、西北内陆棉区的生产示范应用情况;提出了我国早熟棉育种的研究展望,为我国早熟棉育种提供参考。

关键词：棉花早熟性发展应用

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陆地棉早熟性、产量、纤维品质性状相关性及其遗传基础分析

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华北农学报 2021年第1期36卷 95-101页

作者：贾晓昀王寒涛魏恒玲喻树迅中国农业科学院棉花研究所棉花生物学国家重点实验室河南安阳455000 河北省农林科学院粮油作物研究所河北省作物遗传育种实验室河北石家庄050035

为研究陆地棉早熟性、产量、纤维品质之间相互影响的分子遗传基础,以中棉所36为母本、G2005为父本构建重组自交系群体,连续6 a调查群体早熟性、产量、纤维品质相关的13个性状,分析3类性状之间的相关性,并结合QTL定位结果分析其遗传基础... 详细信息

为研究陆地棉早熟性、产量、纤维品质之间相互影响的分子遗传基础,以中棉所36为母本、G2005为父本构建重组自交系群体,连续6 a调查群体早熟性、产量、纤维品质相关的13个性状,分析3类性状之间的相关性,并结合QTL定位结果分析其遗传基础。结果表明,早熟性较好的材料,其铃重较低、纤维品质相对较差;纤维品质较好的材料,其产量性状相对较差;共检测到494个QTL位点,发现68个QTL重叠区段至少影响两类性状,包括11个同时影响早熟性、产量和纤维品质性状的区段,11个同时影响早熟性和产量性状的区段,39个同时影响早熟性和品质性状的区段,7个同时影响产量和纤维品质性状的区段,仅有24个区段内QTL增效基因来源相同。此外,共检测到99个多环境上位性QTL,其中45个位点与加性QTL重叠。

关键词：陆地棉早熟性产量纤维品质相关性分析遗传解析

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陆地棉开花相关基因GhFLP1的克隆与功能验证

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棉花学报 2016年第3期28卷 199-207页

作者：张盼范术丽宋美珍庞朝友魏恒玲喻树迅西北农林科技大学农学院陕西杨陵712100 中国农业科学院棉花研究所/棉花生物学国家重点实验室河南安阳455000

为了明确陆地棉开花促进因子基因的作用,以中棉所36叶片基因组DNA和cDNA为材料克隆得到了开花相关基因Gh FLP1,分析了其特征及功能。该基因全长为537 bp,包含339 bp开放阅读框,编码112个氨基酸。预测分子量约为12.176 kDa,理论等电点为9... 详细信息

为了明确陆地棉开花促进因子基因的作用,以中棉所36叶片基因组DNA和cDNA为材料克隆得到了开花相关基因Gh FLP1,分析了其特征及功能。该基因全长为537 bp,包含339 bp开放阅读框,编码112个氨基酸。预测分子量约为12.176 kDa,理论等电点为9.13。组织特异性表达分析表明,该基因在花蕾中优势表达,且在早熟品种(中棉所36、中棉所74)中表达量高于中熟品种(中棉所60、鲁棉研28)。启动子序列分析和外源激素处理实验表明该基因受水杨酸和赤霉素调控。农杆菌介导转化拟南芥发现,该基因能促使拟南芥莲座叶减少,开花期提前。荧光定量结果表明,在转基因拟南芥中,内源开花相关基因AtFT、AtLFY、AtAP1和AtSOC1表达量不同程度上调,AtFUL表达量基本不变,AtFLC表达量下调。研究结果显示该基因可能参与陆地棉开花时间的调控,为创制转基因早熟棉花新材料打下基础。

关键词： GhFLP1 Gossypium hirsutum 开花基因克隆基因表达荧光定量检测

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陆地棉GhWRKY33的克隆及抗旱功能分析

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中国农业科学 2020年第22期53卷 4537-4549页

作者：魏鑫王寒涛魏恒玲付小康马亮芦建华王省芬喻树迅河北农业大学/棉花生物学国家重点实验室河北基地中国农业科学院棉花研究所/棉花生物学国家重点实验室

【目的】通过克隆陆地棉GhWRKY33并研究其抗旱功能,为棉花抗旱机制解析及分子育种奠定基础。【方法】利用同源克隆的方法从陆地棉中棉所10号中克隆GhWRKY33的开放读码框(open reading frame,ORF),并进行生物信息学分析,分析该基因的二... 详细信息

【目的】通过克隆陆地棉GhWRKY33并研究其抗旱功能,为棉花抗旱机制解析及分子育种奠定基础。【方法】利用同源克隆的方法从陆地棉中棉所10号中克隆GhWRKY33的开放读码框(open reading frame,ORF),并进行生物信息学分析,分析该基因的二级结构、亲疏水性,预测磷酸化位点和启动子区域的顺式作用元件,在NCBI中通过BLASTP检索同源性高的蛋白序列进行序列比对并构建系统发育树;构建35S::GhWRKY33-GFP融合表达载体,通过农杆菌介导注射烟草叶片,观察荧光信号;利用qRT-PCR检测基因的组织表达特异性,以及干旱、ABA、JA、ET处理下的基因表达模式;构建GhWRKY33过表达载体并转化拟南芥,利用20%PEG6000对野生型和T3代转基因拟南芥进行干旱处理,观察处理后野生型和转基因拟南芥的表型,并测定脯氨酸和丙二醛含量等生理生化指标,分析目的基因与干旱响应基因AtP5CS、AtRD29A、AtCOR15A的表达水平。【结果】从陆地棉品种中棉所10号克隆获得GhWRKY33的ORF全长为1 533 bp,编码一个含510个氨基酸残基的蛋白,含有2个WRKY保守结构域和2个C2H2型锌指结构,属于第Ⅰ类WRKY转录因子家族;二级结构预测其编码的蛋白主要由无规则卷曲构成,含有26个苏氨酸磷酸化位点,推测可能与磷酸化有密切的关系,亲疏水性预测表明该蛋白属于亲水性蛋白;系统发育树分析显示该蛋白与GrWRKY33同源性最高。亚细胞定位将GhWRKY33定位于细胞核。qRT-PCR显示该基因具有明显的组织表达特异性,在棉花顶芽的表达量最高;干旱、ABA、JA、ET处理后,基因的表达量明显上升。干旱胁迫后,与野生型相比,转基因拟南芥的抗旱性水平明显提高,植株萎蔫程度较轻,其脯氨酸含量显著升高,丙二醛含量显著降低,且目的基因与干旱响应基因AtP5CS、AtRD29A、AtCOR15A的表达水平均明显提高,表明PEG诱导了该基因的表达,进而调控了干旱响应基因表达上调,使转基因拟南芥表现出对干旱胁迫的抗性。【结论】GhWRKY33响应干旱胁迫,过表达后能明显提高转基因拟南芥的抗旱性。

关键词：陆地棉 GhWRKY33 胁迫抗旱分析

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棉花芽黄材料主要光合特性和农艺性状的研究

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棉花学报 2014年第6期26卷 531-538页

作者：宋明梅范术丽庞朝友魏恒玲喻树迅宋美珍中国农业科学院棉花研究所/棉花生物学国家重点实验室河南安阳455000

以v1、v2、v3、v4、v5v6、v8、v9、v10、v11、v13、v14、v15、v16v17、v18、v19、vg和彭泽芽黄等17份芽黄突变体材料及中棉所58突变体中58vsp为研究对象,测定了各材料不同时期不同叶位的SPAD值、叶绿素荧光参数最大光化学效率值(Fv/Fm)... 详细信息

以v1、v2、v3、v4、v5v6、v8、v9、v10、v11、v13、v14、v15、v16v17、v18、v19、vg和彭泽芽黄等17份芽黄突变体材料及中棉所58突变体中58vsp为研究对象,测定了各材料不同时期不同叶位的SPAD值、叶绿素荧光参数最大光化学效率值(Fv/Fm)和光系统Ⅱ量子产量(φPSⅡ),同时对各芽黄突变体材料的主要农艺性状和纤维品质性状进行了调查和研究。结果表明,芽黄材料作为一种叶色突变体,其叶片相对叶绿素含量在不同时期、不同叶位的差异较大,各芽黄材料均以倒5叶或倒4叶的SPAD值最高,刚展平的倒2叶的SPAD值最小,其中中58vsp的倒2叶SPAD值最低;随着植株的发育,所有芽黄材料在7月5日、15日其倒4、倒5叶的SPAD值达到最大。与光合作用密切相关的最大光化学效率值(Fv/Fm)和PSⅡ量子产量(φPSⅡ)在各芽黄突变体材料之间差异也较大,其中v2最高,v3、v19最低。另外还发现各芽黄突变体材料的主要农艺性状和纤维品质性状存在较大的差异。本文对于不同芽黄突变体材料特性的初步研究,将为更好地将芽黄突变体用于棉花遗传育种和分子生物学研究打下了理论基础。

关键词：棉花芽黄材料 SPAD值

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