Apical meristem transcriptome analysis identifies a role for the blue light receptor gene GhFKF1 in cotton architecture development

作     者：Xiao Li Yuanlong Wu Zhenping Liu Hengling Wei Hantao Wang Shuxun Yu 

作者机构：National Key Laboratory of Crop Genetic ImprovementHubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070HubeiChina State Key Laboratory of Cotton BiologyInstitute of Cotton Research of the Chinese Academy of Agricultural SciencesAnyang 455000HenanChina State Key Laboratory of Subtropical SilvicultureZhejiang A&F UniversityLin’an 311300ZhejiangChina Xianghu LaboratoryHangzhou 311200ZhejiangChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第4期

页      面：1126-1136页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：funded by the National Key Research and Development Program of China(2020YFD1001004) the China Agricultural Research System(CARS-15-06) 

主　　题：Cotton Axillary meristem GhFKF1 GhGI Plant architecture 

摘      要：Cotton architecture is determined by the differentiation fate transition of axillary meristem(AM),and influences cotton yield and the efficiency of mechanized *** observed that the initiation of flowering primordium was earlier in early-maturing than that in late-maturing cultivars during the differentiation and development of *** RNA-Seq and expression level analyses showed that genes FLAVIN BINDING,KELCH REPEAT,F-BOX1(GhFKF1),and GIGANTEA(GhGI)were in response to circadian rhythms,and involved in the regulation of cotton *** gene structure,predicted protein structure,and motif content analyses showed that in Arabidopsis,cotton,rapseed,and soybean,proteins GhFKF1 and GhGI were functionally conserved and share evolutionary *** to the wild type,in GhFKF1 mutants that were created by the CRISPR/Cas9 system,the initiation of branch primordium was ***,the knocking out of GhGI increased the number of AM differentiating into flower primordium,and there were much more lateral branch differentiation and ***,we investigated that proteins GhFKF1 and GhGI can interact with each *** results suggest that GhFKF1 and GhGI are key regulators of cotton architecture development,and may collaborate to regulate the differentiation fate transition of AM,ultimately influencing plant *** describe a strategy for using the CRISPR/Cas9 system to increase cotton adaptation and productivity by optimizing plant architecture.