检索结果-南通市图书馆

Chinese Chemical Letters 2005年第12期16卷 1637-1640页

作者： Hai Yan wang Yan Mei LI Yi xiao Yu fen ZHAO The Key Laboratory of Bioorganic Phosphorus Chemistry ＆ Chemical Biology （Ministry of Education） Department of Chemistry Tsinghua University Beijing 100084

The spontaneous condensation of the amphiphilic N-（O,O-dihexadecyl）phosphorylalanine on the cast film was observed by in situ investigation of transmission infrared spectroscopy The particular orientation and ordered packing of the monomers within the multilayers of the cast film is concluded to attribute to the spontaneous condensation between the monomers.

关键词： Amphiphile cast film condensation FT-IR N-(O O-dihexadecyl)phosphoryl alanine

Structure and Quantum Chemistry Study on Hexaacetyl D-Mannose Hydrazine

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2007年第8期26卷 911-916页

作者： CAO Rui LIU xiao-Hong CHENG Chang-Mei wang Ru-Ji ZHAO Yu-fen wang Lai-Xi Key Laboratory of Bioorganic Phosphorus Chemistry ＆ Chemical Biology （Ministry of Education） Department of Chemistry Tsinghua University Beijing 100084 China Institute of Human Virology University of Maryland Biotechnology Institute University of Maryland USA

Hexaacetyl D-mannose hydrazine is one type of important intermediates in saccharide chemistry. In this paper, its single crystal was obtained and furthermore, X-ray diffraction and quantum chemistry calculation were performed. It belongs to orthorhombic system, space group P212121, with a=16.267（3）, b=19.263（3）, c=7.1948（12）A, Mr=446.41, Dc=1.315 g/cm^3, V=2254.5（6）A^3 and Z=4. Meanwhile, the experimental results also provide information for designing a kind of molecular switch based on the mannose nitrogenous derivatives.

关键词： hexaaeetyl D-mannose hydrazine crystal structure calculation of quantum chemistry molecular switch

长刺蒺藜草两异型种子形态和萌发过程的生理动态特征比较

在线全文

学校读者我要写书评

暂无评论

草业学报 2024年第9期33卷 40-50页

作者：郝丽芬徐晓阳季玉王瑞吴德宝李宇宇林克剑农业农村部人工草地生物灾害监测与绿色防控重点实验室中国农业科学院草原研究所内蒙古呼和浩特010010 植物病虫害生物学国家重点实验室中国农业科学院植物保护研究所北京100193 安徽农业大学植物保护学院安徽合肥230036

长刺蒺藜草刺苞内含有M型和P型两异型种子,两异型种子萌发具有异时性,是其适应异质性环境的策略。为了揭示导致两异型种子异时萌发的内在生理因素,本研究首先比较了两异型种子形态结构和萌发特性指标,同时测定了萌发过程中(干燥、吸胀... 详细信息

长刺蒺藜草刺苞内含有M型和P型两异型种子,两异型种子萌发具有异时性,是其适应异质性环境的策略。为了揭示导致两异型种子异时萌发的内在生理因素,本研究首先比较了两异型种子形态结构和萌发特性指标,同时测定了萌发过程中(干燥、吸胀、露白和出芽期)的生理指标。结果表明,长刺蒺藜草异型种子(M型、P型、刺苞内M型)千粒重、吸水量和萌发率的变化趋势为:刺苞内M型>M型>P型;含水量的变化趋势为:P型>M型>刺苞。P型种子比M型种子种皮致密,淀粉粒出现水解孔滞后,且水解孔更小;干燥期P型种子的脱落酸(ABA)含量显著高于M型种子,而露白期P型种子赤霉素(GA_(4))含量高于M型种子;M型种子可溶性蛋白含量在干燥、吸胀和露白阶段均高于P型种子;在萌发前期(干燥和吸胀期)两异型种子可溶性糖含量无显著差异,在露白和出芽期P型种子可溶性糖含量显著高于M型种子;干燥、吸胀和露白期M型种子葡萄糖-6-磷酸脱氢酶(G6PDH)活性显著高于P型种子,出芽期P型种子显著高于M型种子。综上所述,M型种子先于P型种子萌发,这与种皮结构、ABA/GA_(4)、可溶性蛋白含量和G6PDH活性显著相关,与可溶性糖基础含量无关,M型种子和P型种子的内在萌发机制存在明显差异,可为制定长刺蒺藜草种子引发剂提供理论指导。

关键词：异型种子萌发形态结构脱落酸赤霉素可溶性蛋白可溶性糖葡萄糖-6-磷酸脱氢酶

维普期刊数据库博看期刊

Chemical and chemoenzymatic stereoselectivesynthesis of β-nucleosides and their analogues

在线全文

学校读者我要写书评

暂无评论

Organic Chemistry Frontiers 2022年第6期9卷 1719-1741页

作者： Hui-Jing wang Yang-Yang Zhong You-Cai xiao fen-Er Chen Sichuan Research Center for Drug Precision Industrial Technology West China School of PharmacySichuan UniversityChengdu610041China Engineering Center of Catalysis and Synthesis for Chiral Molecules Department of ChemistryFudan UniversityShanghai200433China Shanghai Engineering Center of Industrial Asymmetric Catalysis for Chiral Drugs Shanghai 200433ChinaShanghai200433China

β-Nucleosides are fundamental building blocks of biological systems and are widely used as therapeuticagents for the treatment of cancer and viral infections, among others. In the last two years, nucleosideanalogues have been investigated with renewed urgency in the search for agents that are effective againstSARS-CoV-2, the cause of the ongoing global pandemic of COVID-19. This has resulted in an explosionof activities in the field of β-nucleoside synthesis. This review summarizes the historical perspective andthe recent advances in the stereoselective synthesis of β-nucleosides and their analogues. The syntheticstrategies to obtain β-nucleosides can be divided into three categories: (1) N-glycosylation;(2) intramolecular sugar ring formation;and (3) enzymatic transglycosylation.

关键词： synthesis analogues nucleoside

Acid-sensing ion channels: potential therapeutic targets for neurologic diseases

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Translational Neurodegeneration 2015年第1期4卷 68-75页

作者： Sha Liu xiao-Yu Cheng fen wang Chun-feng Liu Department of Neurology the Second Affiliated Hospital of Soochow UniversitySoochow University1055 Sanxiang RoadSuzhou 215004China Institute of Neuroscience Soochow UniversitySuzhou 215123China Beijing Key Laboratory for Parkinson’s Disease Beijing 100053China

Maintaining the physiological pH of interstitial fluid is crucial for normal cellular *** disease states,tissue acidosis is a common pathologic change causing abnormal activation of acid-sensing ion channels(ASICs),which according to cumulative evidence,significantly contributes to inflammation,mitochondrial dysfunction,and other pathologic mechanisms(i.e.,pain,stroke,and psychiatric conditions).Thus,it has become increasingly clear that ASICs are critical in the progression of neurologic *** review is focused on the importance of ASICs as potential therapeutic targets in combating neurologic diseases.

关键词： Acid sensing ionic channels Neurologic diseases Pathophysiology Neurodegeneration

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

夏直播棉花产量与冠层微环境的关系

作物学报 2024年第9期50卷 2371-2382页

作者：彭杰谢晓麒张钊姚晓芬邱深陈丹丹顾晓娜王玉洁王晨晨杨国正华中农业大学植物科学技术学院湖北武汉430070

夏直播棉花能够在较短生育期内获得与常规模式相当的产量,但其产量高效形成的机理及其与冠层微环境的关系仍不清楚。因此,本试验采用完全随机区组设计,设置6个种植密度(7.50株m^(–2)、8.25株m^(–2)、9.00株m^(–2)、9.75株m^(–2)、10... 详细信息

夏直播棉花能够在较短生育期内获得与常规模式相当的产量,但其产量高效形成的机理及其与冠层微环境的关系仍不清楚。因此,本试验采用完全随机区组设计,设置6个种植密度(7.50株m^(–2)、8.25株m^(–2)、9.00株m^(–2)、9.75株m^(–2)、10.50株m^(–2)和11.25株m^(–2)),于2022-2023年在湖北武汉进行了大田试验。采用温湿度仪记录从现蕾到拔杆期间冠层温度和湿度,冠层分析仪测定结铃期冠层不同高度光辐射强度,手工收获计产。结果表明,棉花产量随种植密度增加先升后降,其中9.75株m^(–2)最高(籽棉3246.5 kg hm^(–2)、皮棉1203.2 kg hm^(–2)), 2年平均较7.50株m^(–2)提高(15.0%和17.8%)。冠层日均温、最高温、最低温随种植密度增加而降低,而冠层相对湿度、最高湿度、最低湿度随种植密度增加而增加。冠层透光率,水平方向呈“V”型分布,棉行中间最大;垂直方向中下部(离地10^50cm)随种植密度增加而减小,上部(离地50^70cm)随种植密度增加而增大。群体叶面积指数随种植密度增加先升后降,其中9.75株m^(–2)最高,2年平均为2.6。相关分析表明,棉花产量随冠层日均温和最高温升高而降低,随冠层相对湿度和最高湿度增加而增加;随冠层中下部透光率增大而减小,随上部透光率增加而增大。综上,棉花夏直播种植模式,适当增加种植密度(9.75^11.25株m^(–2))有利于提高单位面积成铃数,改善冠层微环境,如冠层透光率70cm处85%以上,50 cm处37%^40%,30 cm处14%^16%,10 cm处9%以下,冠层温度26.5^27.0℃,冠层湿度74%^77%,从而提高棉花产量。

关键词：夏直播产量冠层微环境种植密度叶面积指数

维普期刊数据库博看期刊

在线全文

学校读者我要写书评

暂无评论

基于SNP芯片检测皖南花猪保种群遗传结构

农业生物技术学报 2024年第8期32卷 1869-1877页

作者：王静琳吴旭东何志兵刘阳光王小芬丁月云郑先瑞张晓东殷宗俊安徽农业大学动物科技学院安徽省农业科学院畜牧兽医研究所

皖南花猪(Sus scrofa domesticus)是安徽省乃至中国不可或缺的宝贵猪种资源，研究皖南花猪保种群体的遗传多样性、亲缘关系和家系结构，对揭示皖南花猪目前的群体状况至关重要。本研究基于“中芯一号”芯片(Chinese Chip-1 BeadChip)检... 详细信息

皖南花猪(Sus scrofa domesticus)是安徽省乃至中国不可或缺的宝贵猪种资源，研究皖南花猪保种群体的遗传多样性、亲缘关系和家系结构，对揭示皖南花猪目前的群体状况至关重要。本研究基于“中芯一号”芯片(Chinese Chip-1 BeadChip)检测32头皖南花猪的全基因组SNP位点，利用PLINK软件进行观察杂合度(observed heterozygosity, Ho)、期望杂合度(expected heterozygosity, He)和多态信息含量(polymorphic information content, PIC)的计算及连续性纯合片段(runs of homozygosity, ROH)的检测，分析皖南花猪群体的遗传多样性。通过GCTA软件构建G矩阵，解析皖南花猪群体的亲缘关系；MEGA X软件构建群体进化树，分析皖南花猪群体的家系结构。结果显示，32头皖南花猪共检测到42 662个SNPs位点，满足质控条件的SNP位点有21 317个；群体多态性标记比例(polymorphism marker ratio, PN)为0.500，有效群体含量(effective population size, Ne)为3，群体He为0.348,Ho为0.372，平均亲缘系数为0.11。G矩阵的结果显示，大多数皖南花猪之间存在中等程度的亲缘关系。ROH片段共有884个，ROH平均长度为(12.35±2.17) Mb，个体ROH平均长度为(341.19±139.58) Mb；基于ROH的平均近交系数为0.139±0.056，保种群的近交程度较低；聚类分析结果表明，皖南花猪公猪来源于5个血统。综上所述，皖南花猪保种群群体有效含量偏低，遗传多样性中等，近交程度中等，可能含有少量外来血缘。本研究为皖南花猪遗传资源的保护及利用提供科学依据。

关键词：皖南花猪 SNP芯片遗传结构亲缘关系家系结构

Neuroprotective effect of Cerebralcare Granule after cerebral ischemia/reperfusion injury

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第4期11卷 623-629页

作者： xiao-xiao Zhang fen-fen He Gui-lin Yan Ha-ni Li Dan Li Yan-ling Ma Fang wang Nan Xu Fei Cao Department of Neurology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Neurology Liyuan HospitalTongji Medical CollegeHuazhong University of Science and Technology

Cerebralcare Granule（CG） improves cerebral microcirculation and relieves vasospasm,but studies investigating its therapeutic effect on cerebral ischemia/reperfusion injury are *** the present study,we administered CG（0.3,0.1 and 0.03 g/m L intragastrically） to rats for 7 consecutive *** then performed transient occlusion of the middle cerebral artery,followed by reperfusion,and administered CG daily for a further 3 or 7 *** with no treatment,high-dose CG markedly improved neurological function assessed using the Bederson and Garcia *** 3 days,animals in the high-dose CG group had smaller infarct volumes,greater interleukin-10 expression,and fewer interleukin-1β-immunoreactive cells than those in the untreated model ***,at 7 days,high-dose CG-treated rats had more vascular endothelial growth factor-immunoreactive cells,elevated angiopoietin-1 and vascular endothelial growth factor expression,and improved blood coagulation and flow indices compared with untreated model *** results suggest that CG exerts specific neuroprotective effects against cerebral ischemia/reperfusion injury.

关键词： nerve regeneration Cerebralcare Granule cerebral ischemia/reperfusion angiogenesis inflammatory response blood coagulation hemorheology neural regeneration

Highly Selective Preparation of 3-and 6-Modified Mannose/Altrose Derivatives

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2006年第3期22卷 339-342页

作者： LIU xiao-hong XIE Dang CHENG Chang-mei wang Xia-yu GUO xiao-qiang ZHAO Yu-fen Key Laboratory of Bioorganic Phosphorous Chemistry and Chemical Biology of Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 P.R. China Institute of Polymer Science and Engineering Xiangtan University Xiangtan 411105 P.R. China

A highly efficient method to get 3-and 6-modified mannose/ahrose derivatives for oligosaccharide synthesis is described. All the compounds were obtained exclusively with the combinations of 3-and/or 6-hydroxyl protect... 详细信息

关键词： Mannose Altrose Selective protection Selective deprotection

RAD51C表达下调对小鼠卵巢癌体内成瘤和VEGF、NRP-2表达的调控

在线全文

学校读者我要写书评

暂无评论

南昌大学学报（医学版） 2024年第5期64卷 44-49,68页

作者：卢小丽李夏影王芬刘丝荪芦春斌广州中医药大学第一附属医院中山医院妇产科广东中山528463 南昌大学第一附属医院妇产科南昌330006 暨南大学发育生物学与再生医学广州510632

目的探讨RAD51旁系同源基因C(RAD51C)表达下调对小鼠卵巢癌体内成瘤和血管内皮生长因子(VEGF)、神经纤毛蛋白-2(NRP-2)表达调控的影响。方法使用A2780卵巢癌细胞,通过培养检测细胞中RAD51C的表达,构建3种RAD51C干扰载体(SiRNA-47、SiRNA... 详细信息

目的探讨RAD51旁系同源基因C(RAD51C)表达下调对小鼠卵巢癌体内成瘤和血管内皮生长因子(VEGF)、神经纤毛蛋白-2(NRP-2)表达调控的影响。方法使用A2780卵巢癌细胞,通过培养检测细胞中RAD51C的表达,构建3种RAD51C干扰载体(SiRNA-47、SiRNA-183和SiRNA-285),并包装成慢病毒,转染细胞,逆转录实时定量聚合酶链式反应(RT-qPCR)验证转染效果,进行稳筛,构建稳转细胞株。使用Balb/c雌性裸鼠建立细胞系来源的异体移植肿瘤模型(CDX)模型,将18只建模成功的裸鼠分为3组:A2780细胞组(Control组)、A2780细胞+空载体对照组(NC组)、A2780细胞+RAD51C干扰组(Si-RAD51C组),每组各6只。Control组注射生理盐水,NC组注射空载慢病毒50μL,Si-RAD51C组注射RAD51C干扰慢病毒50μL。观察各组成瘤情况,取肿瘤组织,采用蛋白质印迹法(WB)、免疫组织化学检测RAD51C、VEGF、NRP-2蛋白表达情况。结果RT-qPCR验证RAD51C干扰慢病毒转染效果以SiRAN-285最明显(P<0.05)。Si-RAD51C组与Control组、NC组比较瘤体的体积最小、重量也最轻,且RAD51C、NRP-2及VEGF蛋白的表达显著降低(P<0.05)。结论RAD51C干扰慢病毒可抑制小鼠A2780卵巢癌细胞肿瘤的形成,且对RAD51C、NRP-2及VEGF蛋白的表达均有抑制作用。

关键词： RAD51旁系同源基因C 卵巢癌血管内皮生长因子神经纤毛蛋白-2 裸鼠成瘤模型体外实验小鼠