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Molecular phylogeny of Bactrocera species （Diptera： Tephritidae） inferred from mitochondrial 16s rDNA sequences

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Journal of Agricultural Science and Technology 2009年第6期3卷 24-28页

作者： ZHANG Bin LIU Ying-hong wang ze-le ZHAO Lan-lan Chongqing Key Laboratory of Entomology ＆ Insect Control Engineering College of Plant Protection Southwest University Chongqing 400013 China Chongqing Agriculture Technique Extension Station Chongqing 400013 China

The fruit flies of the Bactrocera （Diptera： Tephritidae） are one of the major economically important insects in Asia. So far, there are at least seven species found in Chongqing, China. By using mitochondrial 16s rDNA sequences, the phylogenetic relationships among seven Bactrocera species, Bactrocera cucurbitae （Coquillett）, B. tau （Walker）, B. diaphora （Hendel）, B. caudata （Fabricius）, B. scutellata （Hendel）, B. dorsalis （Hendel）, and B. minax （Enderlein） were investigated. Nucleotide diversity within species ranged from 0.3% to 10.9%. According to the result, B. cucurbitae （Coquillett）, B. tau （Walker）, B. diaphora （Hendel）, and B. caudata （Fabricius） have no enough sites to be distinguished among the other species in the 347bp nucleotide sequences of the 16s rDNA gene. At the same time, excluding B. scutellata （Hendel） and B. minax （Enderlein）, the other species recognition sites are not only too little but also discontinuous. Phylogenetic trees calculated from both maximum parsimony and neighbor-joining phylogenetic analysis methods showed that B. cucurbitae and B. tau were closely related, B. diaphora （Hendel） and B. caudata （Fabricius） also had a close relationship. B. minax （Enderlein） has a furthest relationship from the other 6 species. From the results, we can infer that the our data supports previous classification of Bactrocera based on morphological characters in other words, but the Bactrocera subgenera, zeugodacus is polyphyletic.

关键词： Bactrocera spp 16s rDNA mitochondrial DNA molecular phylogeny

MXene Hybridized Polymer with Enhanced Electromagnetic Energy Harvest for Sensitized Microwave Actuation and Self-Powered Motion Sensing

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NANO-MICRO leTTERS 2025年第1期17卷 1-15页

作者： wang, Yu-ze wang, Yu-Chang Liu, Ting-Ting Zhao, Quan-Liang Li, Chen-Sha Cao, Mao-Sheng Beijing Inst Technol Sch Mat Sci & Engn Beijing 100081 Peoples R China Peking Univ Sch Mat Sci & Engn Beijing 100871 Peoples R China North China Univ Technol Sch Mech & Mat Engn Beijing 100144 Peoples R China Heilongjiang Univ Minist Educ Peoples Republ China Key Lab Funct Inorgan Mat Chem Harbin 150080 Peoples R China

Polymeric microwave actuators combining tissue-like softness with programmable microwave-responsive deformation hold great promise for mobile intelligent devices and bionic soft robots. However, their application is challenged by restricted electromagnetic sensitivity and intricate sensing coupling. In this study, a sensitized polymeric microwave actuator is fabricated by hybridizing a liquid crystal polymer with Ti3C2Tx (MXene). Compared to the initial counterpart, the hybrid polymer exhibits unique space-charge polarization and interfacial polarization, resulting in significant improvements of 230% in the dielectric loss factor and 830% in the apparent efficiency of electromagnetic energy harvest. The sensitized microwave actuation demonstrates as the shortened response time of nearly 10 s, which is merely 13% of that for the initial shape memory polymer. Moreover, the ultra-low content of MXene (up to 0.15 wt%) benefits for maintaining the actuation potential of the hybrid polymer. An innovative self-powered sensing prototype that combines driving and piezoelectric polymers is developed, which generates real-time electric potential feedback (open-circuit potential of similar to 3 mV) during actuation. The polarization-dominant energy conversion mechanism observed in the MXene-polymer hybrid structure furnishes a new approach for developing efficient electromagnetic dissipative structures and shows potential for advancing polymeric electromagnetic intelligent devices.

关键词： Microwave absorption Electromagnetic response Energy harvest Self-sensing Soft actuator

重庆市26个南亚果实蝇种群mtDNA 16S rRNA基因部分序列及其系统进化

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昆虫知识 2007年第4期44卷 556-561页

作者：王泽乐刘映红刘洪张昌伦王梓英西南大学植物保护学院重庆市昆虫学及害虫控制工程重点实验室重庆400715 重庆市农业技术推广总站重庆400020 重庆市沙坪坝区植保植检站重庆400030

对重庆市26个南亚果实蝇Bactrocera(zeugodacus)tau(Walker)种群线粒体16S rRNA基因进行测序,获得长约350bp片段的序列。对获得的序列分析表明,A,T,C,G平均含量分别为35·0%,41·3%,7·2%,16·5%,其中保守位点数342个,变异位点数5个,简... 详细信息

对重庆市26个南亚果实蝇Bactrocera(zeugodacus)tau(Walker)种群线粒体16S rRNA基因进行测序,获得长约350bp片段的序列。对获得的序列分析表明,A,T,C,G平均含量分别为35·0%,41·3%,7·2%,16·5%,其中保守位点数342个,变异位点数5个,简约信息位点2个,自裔位点2个,所有碱基转换总数为136,替换总数为50。利用MEGA2·1软件重建系统发生树,发现其中21个南亚果实蝇种群未出现分化,另外有5个南亚果实蝇种群出现了分化,但遗传分化程度小。

关键词：南亚果实蝇种群线粒体基因 16S rRNA 系统进化

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矿用架线机车智能气动控制系统

液压与气动 2005年第6期29卷 43-45页

作者：宋志安王文馨李成银王则乐山东科技大学机电学院翟镇煤矿

文章根据产生式知识表达方式构造自动刹车和温控智能控制系统,实现了矿用架线机车遇红灯信号的自动断电刹车,避免了电机失火事故的发生。

关键词：矿用架线机车产生式规则温度控制制动

Microglia lactylation in relation to central nervous system diseases

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Neural Regeneration Research 2025年第1期20卷 29-40页

作者： Hui Yang Nan Mo le Tong Jianhong Dong Ziwei Fan Mengxian Jia Juanqing Yue Ying wang School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Clinical Laboratory The Fourth Clinical Medical College of Zhejiang University of Traditional Chinese Medicine(Hangzhou First People’s Hospital)HangzhouZhejiang ProvinceChina College of Medical Veterinary and Life SciencesUniversity of GlasgowGlasgowUK Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalWestlake University School of MedicineHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalWestlake University School of MedicineHangzhouZhejiang ProvinceChina

The development of neurodegenerative diseases is closely related to the disruption of central nervous system ***,as innate immune cells,play important roles in the maintenance of central nervous system homeostasis,injury response,and neurodegenerative *** has been considered a metabolic waste product,but recent studies are revealing ever more of the physiological functions of *** is an important pathway in lactate function and is involved in glycolysis-related functions,macrophage polarization,neuromodulation,and angiogenesis and has also been implicated in the development of various *** review provides an overview of the lactate metabolic and homeostatic regulatory processes involved in microglia lactylation,histone versus non-histone lactylation,and therapeutic approaches targeting ***,we summarize the current research on microglia lactylation in central nervous system diseases.A deeper understanding of the metabolic regulatory mechanisms of microglia lactylation will provide more options for the treatment of central nervous system diseases.

关键词： brain central nervous system glycolysis immune response inflammation lactate metabolism lactate lactylation microglia neurodegenerative diseases

分子生物学技术在双翅目实蝇科昆虫系统发育研究中的应用

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昆虫知识 2006年第5期43卷 603-607页

作者：王泽乐刘映红李小珍刘明圣裴明玉西南大学植物保护学院重庆市昆虫学及害虫控制工程重点实验室重庆400716

分子生物学技术如同工酶电泳、RFLP、RAPD、核酸序列分析、微卫星DNA和探针杂交等,在实蝇科昆虫系统发育研究中具有重要作用。利用这些技术对实蝇种群进行系统发育研究,揭示其亲缘及进化关系,从生命本质上寻找实蝇种群间的内在联系。文... 详细信息

分子生物学技术如同工酶电泳、RFLP、RAPD、核酸序列分析、微卫星DNA和探针杂交等,在实蝇科昆虫系统发育研究中具有重要作用。利用这些技术对实蝇种群进行系统发育研究,揭示其亲缘及进化关系,从生命本质上寻找实蝇种群间的内在联系。文章综述上述几种分子生物学技术在实蝇科昆虫核酸结构、种内和种间的亲缘及进化关系等方面的研究进展,分析在应用中存在的问题,展望这些分子生物学技术在实蝇科昆虫系统发育中的应用前景。

关键词：分子生物学技术实蝇科系统发育系统发育树

Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

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Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen Chen Xiaolin zeng le wang Wenwu Zhang Gang Li Xing Cheng Peiqiang Su Yong Wan Xiang Li Department of Spine Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

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诱剂监测南亚果实蝇在重庆的分布与发生动态

西南农业大学学报（自然科学版） 2006年第2期28卷 309-313,325页

作者：王泽乐刘映红江兴培刘洪裴明玉刘明圣西南大学植物保护学院重庆400716 重庆出入境检验检疫局重庆400020 重庆植物保护检疫站重庆400020

2005年5-10月份利用甲基丁香酚(M ethyleugenol)、诱蝇酮(Cuelure)和水解蛋白(Hydrolytic prote in)3种诱剂监测了重庆市26个区县共计130个点南亚果实蝇Bactrocera tau(W alker)的分布与发生动态。监测结果表明,在重庆市26个区县共诱集... 详细信息

2005年5-10月份利用甲基丁香酚(M ethyleugenol)、诱蝇酮(Cuelure)和水解蛋白(Hydrolytic prote in)3种诱剂监测了重庆市26个区县共计130个点南亚果实蝇Bactrocera tau(W alker)的分布与发生动态。监测结果表明,在重庆市26个区县共诱集到南亚果实蝇68 525头,在各个诱集区县均有分布,其中合川、江津、涪陵、长寿区平均每个诱集点诱集的南亚果实蝇数量大,均在800头以上,是该虫发生严重的区县,城口、渝北、南川、黔江、武隆、永川区平均每个诱集点诱集南亚果实蝇数量小,均不足30头,是该虫发生较轻的区县。除涪陵区外,其他区县在7-9月份出现1个虫口高峰期,涪陵区出现2个虫口高峰期,分别在7月份和9月份。对3种诱剂诱集数量统计分析发现,不同诱剂每个监测点诱集的南亚果实蝇平均数量无显著性差异,从诱集南亚果实蝇的雌雄比例来看,诱蝇醚、诱蝇酮诱剂对南亚果实蝇雄成虫诱集效果好,对雌性个体诱集效果差,而水解蛋白诱剂对南亚果实蝇雌雄成虫均有较好的诱集效果。对6个南亚果实蝇发生数量较大区县的寄主作物分析发现,在其中5个区县中,丝瓜上的诱集数量明显大于其他寄主作物上的诱集数量,说明丝瓜比其他瓜类寄主作物更适合南亚果实蝇的取食选择。

关键词：南亚果实蝇诱剂监测数量动态分布发生

Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening

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Science China Life Sciences 2025年第1期 300-302页

作者： ze-Min Lin Mai Xiang Wen-Hui Wei Shu-Hui Fan Li Chen Jie wang Xiao-Qian Yang Chun-Hao Yang Shi-Jun He Jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences School of Pharmacy University of Chinese Academy of Sciences School of Pharmacy Nanjing University of Chinese Medicine State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of Sciences Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine

Dear Editor, Phenotypic drug discovery, which observes compound effects on physiology,has given way to targeting specific molecules, but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al., 2022). Osteoarthritis(OA) is a prevalent chronic joint disorder, primarily affecting knee joints, with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).

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