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Agricultural Sciences in China 2010年第12期9卷 1726-1738页

作者： yu Yong-tao wang Rong-huan SHI yun-su SONG Yan-chun wang tian-yu LI yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences/National Facility for Gene Resources and Genetic Improvement Beijing 100081 P.R. China Crop Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R.China

Drought stress is one of the most important factors limiting maize production. Rab17 is an ABA-responsive gene and associated with drought tolerance. In order to identify haplotypic structure and mine allelic variants at tab17 locus, nucleotide diversity and linkage disequilibrium （LD） structure of rab17 were evaluated among a mini core set of Chinese diversified maize inbred lines. Totally, 19 SNP and 18 insertion/deletions （InDels） were identified, among which 81% were in non-coding regions and 19% in coding regions. The results showed that a high level of diversity appeared within 1 kb upstream of the rabl 7 locus, and declined quickly downstream of the gene region. Rapid decay of linkage disequilibrium of rabl 7 region with distance within 1 kb was detected. Functional markers which can be developed based on haplotype 14 are expected to have contribution to molecular breeding for drought tolerance.

关键词： allelic variation drought tolerance linkage disequilibrium maize rab17

A device for fluorescence temperature measurement based on fast fourier transform

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Optoelectronics Letters 2008年第2期4卷 147-149页

作者： wang Dong-sheng wang Gui-mei PAN Wei-wei wang yu-tian Hebei Engineering University Hebei 056001 China Yanshan University Qinhuangdao 066004 China

A sapphire fiber thermal probe with Cr^3＋ ion-doped end was grown using the laser heated pedestal method. The fluorescence thermal probe offers advantages of compact structure, high performance and the ability to sustain high temperature from the room temperature to 450℃. Based on the fast fourier transform （FFT）, the fluorescence lifetime is obtained from the tangent function of the phase angle of the first non-zeroth item of FFT result. Compared with other traditional fitting methods, our method has advantages such as fast speed, high accuracy and being free from the influence of the base signal. The standard deviation of FFT method is about half of that method. In addition, since the FFT method is immunity to analysis can be skipped. of the Prony method and close to the one of the Marquardt the background noise of the signal, the background noise

关键词：傅立叶转移器件荧光温度测量方法

Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials

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World Journal of Gastroenterology 2009年第8期15卷 919-926页

作者： Xiao-Ping Zhang Wei-Hong wang yu tian Wen Gao Jiang Li Department of Gastroenterology Peking University First Hospital Beijing 100034 China

AIM： To investigate the increasing the susceptibility pylon） to metronidazole. mechanisms of aspirin of Helicobacter pylori （H METHODS： Hpylori reference strain 26695 and two metronidazole-resistant isolates of H pylori were included in this study. Strains were incubated in Brucella broth with or without aspirin （1 mmol/L）. The rdxA gene of Hpylori was amplified by PCR and sequenced. The permeability of Hpylori to antimicrobials was determined by analyzing the endocellular radioactivity of the cells after incubated with [7-^3H]-tetracycline. The outer membrane proteins （OMPs） of Hpylori 26695 were depurated and analyzed by SDS-PAGE. The expression of 5 porins （hopA, hopB, hopC, hopD and hopE） and the putative RND efflux system （hefABC） of H pylori were analyzed using real-time quantitative PCR. RESULTS： The mutations in rdxA gene did not change in metronidazole resistant isolates treated with aspirin. The radioactivity of H pylori increased when treated with aspirin, indicating that aspirin improved the permeability of the outer membrane of H pylori. However, the expression of two OMP bands between 55 kDa and 72 kDa altered in the presence of *** expression of the mRNA of hopA, hopB, hopC, hopD, hopE and herA, hefB, hefC of H pylori did not change when treated with aspirin. CONCLUSION： Although aspirin increases the susceptibility of H pylori to metronidazole, it has no effect on the mutations of rdxA gene of Hpylori. Aspirin increases endocellular concentrations of antimicrobials probably by altering the OMP expression.

关键词： Helicobacter pylori Aspirin Metronidazole Resistance Minimum inhibitory concentrations

Dynamic changes of root proteome reveal diverse responsive proteins in maize subjected to cadmium stress

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Journal of Integrative Agriculture 2019年第10期18卷 2193-2204页

作者： REN Wen LIU Ya ZHOU Miao-yi SHI Zi wang tian-yu ZHAO Jiu-ran LI yu Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Maize Research Center Beijing Academy of Agriculture & Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular BreedingBeijing 100097P.R.China

Toxic symptoms and tolerance mechanisms of heavy metal in maize are well documented. However, limited information is available regarding the changes in the proteome of maize seedling roots in response to cadmium(Cd) stress. Here, we employed an i TRAQ-based quantitative proteomic approach to characterize the dynamic alterations in the root proteome during early developmental in maize seedling. We conducted our proteomic experiments in three-day seedling subjected to Cd stress, using roots in four time points. We identified a total of 733, 307, 499, and 576 differentially abundant proteins after 12, 24, 48, or 72 h of treatment, respectively. These proteins displayed different functions, such as ribosomal synthesis, reactive oxygen species homeostasis, cell wall organization, cellular metabolism, and carbohydrate and energy metabolism. Of the 166 and 177 proteins with higher and lower abundance identified in at least two time points, 14 were common for three time points. We selected nine proteins to verify their expression using quantitative real-time PCR. Proteins involved in the ribosome pathway were especially responsive to Cd stress. Functional characterization of the proteins and the pathways identified in this study could help our understanding of the complicated molecular mechanism involved in Cd stress responses and create a list of candidate gene responsible for Cd tolerance in maize seeding roots.

关键词： cadmium stress iTRAQ proteomics maize seedling roots

Isolation and Analysis of Drought-Induced Genes in Maize Roots

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Agricultural Sciences in China 2009年第2期8卷 129-136页

作者： LI Hui-yong HUANG Shu-hua SHI yun-su SONG Yan-chun ZHONG Zhong-bao wang Guo-ying wang tian-yu LI yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China The Cereal Crops Institute Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China

Maize roots are important component for plant adaptation to soil water deficits because they are supposed to take up water and necessary solutes from the soil. In the present study, the drought-induced genes were isolated in maize roots. A suppression subtractive hybridization protocol was applied to construct a forward subtractive cDNA library from CN165 for drought-stressed maize roots and a number of drought-induced genes were isolated. Totally, 126 uniESTs （containing 82 singlets and 44 contigs） were obtained from 503 available ESTs sequences after macroarray hybridization. UniESTs were analyzed using BLASTN and BLASTX and the results showed that 92% of the uniESTs had homolgous sequences in maize nr database by BLASTN. About 89% of uniESTs appeared the homlogous amino acid sequences in rice protein database but not in maize protein database by BLASTX, implying that those genes are likely new functional genes in maize. Function analysis showed that those genes were involved in a broad spectrum of biological pathways, mainly in signaling and regulatory pathways related to stress tolerance.

关键词： maize roots drought stress suppression subtractive hybridization expressed sequence tag

Changes in grain-filling characteristics of single-cross maize hybrids released in China from 1964 to 2014

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Journal of Integrative Agriculture 2023年第3期22卷 691-700页

作者： GAO Xing LI Yong-xiang YANG Ming-tao LI Chun-hui SONG Yan-chun wang tian-yu LI yu SHI yun-su Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

Grain filling is the physiological process for determining the obtainment of yield in cereal *** grain-filling characteristics of 50 maize brand hybrids released from 1964 to 2014 in China were assayed across multiple *** found that the grain-filling duration(54.46%)and rate(43.40%)at the effective grain-filling phase greatly contributed to the final performance parameter of 100-kernel weight(HKW).Meanwhile,along with the significant increase in HKW,the accumulated growing degree days(GDDs)for the actual grain-filling period duration(AFPD)among the selected brand hybrids released from the 1960s to the 2010s in China had a decadal increase of 23.41℃ ***,there was a decadal increase of only 19.76℃ d for GDDs of the days from sowing to physiological maturity(DPM),which was also demonstrated by a continuous decrease in the ratio between the days from sowing to silking(DS)and DPM(i.e.,from 53.24%in the 1960s to 49.78%in the 2010s).In contrast,there were no significant changes in grain-filling rate along with the release years of the selected ***,the stability of grain-filling characteristics across environments also significantly increased along with the hybrid release *** also found that the exotic hybrids showed a longer grain-filling duration at the effective grain-filling phase and more stability of the grain-filling characteristics than those of the Chinese local *** to the results of this study,it is expected that the relatively longer grain-filling duration,shorter DS,higher grain-filling rate,and steady grain-filling characteristics would contribute to the yield improvement of maize hybrids in the future.

关键词： maize(Zea mays L.) grain-filling rate grain-filling duration stability

Stability of QTL Across Environments and QTL-by-Environment Interactions for Plant and Ear Height in Maize

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Agricultural Sciences in China 2010年第10期9卷 1400-1412页

作者： ZHANG Yan LI Yong-xiang wang wang LIU Zhi-zhai LIU Cheng PENG Bo TAN Wei-wei wang Di SHI yun-su SUN Bao-cheng SONG Yan-chun wang tian-yu LI yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Food Crops Xinjiang Academy of Agricultural Sciences Urumqi 830000 P.R.China Maize Research Institute Southwest University Chongqing 400716 P.R.China

Better understanding of genotype-by-environment interaction （GEI） is expected to provide a solid foundation for genetic improvement of crop productivity especially under drought-prone environments. To elucidate the genetic basis of the plant and ear height, 2 F2：3 populations were derived from the crosses of Qi 319 × Huangzaosi （Q/H） and Ye 478 × Huangzaosi （Y/H） with 230 and 235 families, respectively, and their parents were evaluated under 3 diverse environments in Henan, Beijing, and Xinjiang, China during the year of 2007 and 2008, and all the lines were also evaluated under water stress environment. The mapping results showed that a total of 21 and 12 QTLs were identified for plant height in the Q/H and Y/H population, respectively, and 24 and 13 QTLs for ear height, respectively. About 56 and 73% of the QTLs for 2 traits did not present significant QTL-by-environment interaction （QE1） in the normal joint analyses for Q/H and Y/H population, respectively, and about 73% of the QTLs detected did not show significant QEI according to joint analyses for stress condition in Q/H. Most of the detected major QTLs exhibited high stability across different environments. Besides, several major QTLs were detected with large and consistent effect under normal condition （Chr. 6 and 7 in Q/H; Chr. 1, 3 and 9 in Y/H）, or across 2 water regimes （Chr. 1, 8 and 10 for in Q/H）. There were several constitutive QTLs （3 for Q/H and 1 for Y/H） with no or minor QTL-by-environment for the 2 populations. Finally, we found several genomic regions （Chr. 1, 10, etc.） to be co-located across the populations, which could provide useful reference for genetic improvement of these traits in maize breeding programs. Comparative genomic analysis revealed that 3 genes/genetic segments associated with plant height in rice were orthologous to these 3 identified genomic regions carrying the major QTLs for plant and ear height on Chr. 1, 6, and 8, respectively.

关键词： maize (Zea mays L.) stability QTL QTL-by-environment plant height ear height

Analysis of Genetic Diversity and Population Structure of Maize Landraces from the South Maize Region of China

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Agricultural Sciences in China 2010年第9期9卷 1251-1262页

作者： LIU Zhi-zhai GUO Rong-hua ZHAO Jiu-ran CAI Yi-lin wang Feng-ge CAO Mo-ju wang Rong- huan SHI yun-su SONG Yan-chun wang tian-yu LI yu Maize Research Institute Southwest University Chongqing 400716 P.R. China Institue of Crop Sciences/National Key Facility for Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Maize Research Institute Sichuan Agricultural University Ya＇an 625014 P.R.China Maize Research Center Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 P.R. China

Understanding genetic diversity and population structure of landraces is important in utilization of these germplasm in breeding programs. In the present study, a total of 143 core maize landraces from the South Maize Region （SR） of China, which can represent the general profile of the genetic diversity in the landraces germplasm of SR, were genotyped by 54 DNA microsatellite markers. Totally, 517 alleles （ranging from 4 to 22） were detected among these landraces, with an average of 9.57 alleles per locus. The total gene diversity of these core landraces was 0.61, suggesting a rather higher level of genetic diversity. Analysis of population structure based on Bayesian method obtained the samilar result as the phylogeny neighbor-joining （N J） method. The results indicated that the whole set of 143 core landraces could be clustered into two distinct groups. All landraces from Guangdong, Hainan, and 15 landraces from Jiangxi were clustered into group 1, while those from the other regions of SR formed the group 2. The results from the analysis of genetic diversity showed that both of groups possessed a similar gene diversity, but group 1 possessed relatively lower mean alleles per locus （6.63） and distinct alleles （91） than group 2 （7.94 and 110, respectively）. The relatively high richness of total alleles and distinct alleles preserved in the core landraces from SR suggested that all these germplasm could be useful resources in germplasm enhancement and maize breeding in China.

关键词： maize core landraces genetic diversity population structure

Genetic Diversity of Two Important Groups of Maize Landraces with Same Name in China Revealed by M13 Tailed-Primer SSRs

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Agricultural Sciences in China 2009年第1期8卷 15-23页

作者： LIU Zhi-zhai GUO Rong-hua ZHAO Jiu-ran CAI Yi-lin wang Feng-ge CAO Mo-ju wang Rong-huan SHI yun-su SONG Yan-chun wang tian-yu LI yu Maize Research Institute Southwest University Chongqing 400715 P.R.China Maize Research Institute Sichuan Agricultural University Ya＇an 625014 P.R.China Maize Research Center Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 P.R. China Institute of Crop Sciences Chinese Academy of Agricultural Sciences/National Key Facility for Gene Resources and Genetic Improvement （NT-CRI） Ministry of Agriculture Beijing 100081 P.R.China

Maize landraces White Dent and Golden Queen played a very important role in the pre-hybrid era of maize production in China. However, dozens of accessions with the same names of White Dent and Golden Queen are preserved in China National Genebank （CNG）. The present study investigated the genetic diversity of these two important groups of maize landraces, as well as the relationships within and among them. Thirty-four landrace accessions with the name of White Dent and 10 with Golden Queen preserved in CNG were fingerprinted with 52 simple sequence repeats with tailed primer M13. Summary statistics including average number of alleles per locus, gene diversity/expected heterozygosity, and observed heterozygosity were carried out using PowerMarker ver. 3.25 software. The test of Hardy-Weinberg equilibrium （HWE） and linkage disequilibrium （LD） of all the 44 maize landrace accessions were also performed by PowerMarker. We observed a significant differentiation in terms of the average number of alleles between White Dent and Golden Queen （6.44 alleles per locus in White Dent, 4.48 in Golden Queen）, while both groups of maize landraces had a relatively high but similar gene diversity （0.61 of White Dent, 0.63 of Golden Queen）. The fixation index （FST） was only 0.0044, while the percentage of loci deviated from Hardy-Weinberg equilibrium within these two groups of White Dent and Golden Queen was 32.69 and 3.92%, respectively. The rather high genetic diversity and average number of alleles per locus confirmed that both groups of landraces had a rather broad germplasm base. The extremely low fixation index showed that there was little genetic variation between White Dent and Golden Queen and the molecular variation within these two groups was remarkably high, indicating no genetic drift between White Dent and Golden Queen and suggesting different improvement approaches to these two important groups of landraces. Hardy-Weinberg equilibrium test revealed that the group of White

关键词： maize landrace White Dent Golden Queen genetic diversity M13 tailed-primer SSRs

Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization

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Agricultural Sciences in China 2007年第6期6卷 647-651页

作者： LI Hui-yong HUANG Su-hua SHI yun-su SONG Yan-chun ZHAO Jiu-ran wang Feng-ge wang tian-yu LI yu Institute of Food Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Maize Research Institute Beijing Academy of Agricultural and Forestry Sciences Beijing 100081 P.R.China

In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress.

关键词： maize drought stress suppression subtractive hybridization (SSH) uniESTs