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Mitochondrial genomes of Tapes dorsatus and Cardita variegata:insights into Heteroconchia phylogeny

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Journal of Oceanology and Limnology 2024年第3期42卷 943-959页

作者： Xumin wang Hua ZHANG Xindong TENG Wenhui SUN Zhikai XING shuang wang Xiumei LIU Jiangyong QU Lijun wang College of Life Science Yantai UniversityYantai 264005China Qingdao International Travel Healthcare Center Qingdao 266071China

Heteroconchia,a widespread and abundant aquatic invertebrate,is an important clade of bivalve *** relationship between the three branches of Heteroconchia,Palaeoheterodonta,Archiheterodonta,and Euheterodonta has become a main controversy in molecular studies of the relationships between *** the present study,we assembled the complete mitochondrial genomes of Tapes dorsatus(Veneridae)and Cardita variegata(Carditidae)using high-throughput *** is the first mitochondrial genome belonging to the family Carditidae to be *** used 12 protein coding genes(excluding atp8)from the complete mitochondrial genomes of 146 species to recover the internal relationships of *** results support the traditional view of early branching of Palaeoheterodonta and the recovery of the monophyly of Palaeoheterodonta,Anomalodesmata,*** analysis show that gene arrangement within Venerida was highly ***-calibrated phylogenetic studies based on a relaxed molecular clock model suggested that Veneridae originated approximately 337.62 million years ago(Ma)and split into two major clades,whereas Carditidae originated approximately 510.09 *** results provide evidence of the internal relationships of Heteroconchia.

关键词： Tapes dorsatus Cardita variegata mitochondrial genome phylogeny

MYB106 is a negative regulator and a substrate for CRL3^(BPM) E3 ligase in regulating flowering time in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2021年第6期63卷 1104-1119页

作者： Liu Hong Fangfang Niu Youshun Lin shuang wang Liyuan Chen Liwen Jiang Center for Cell&Developmental Biology State Key Laboratory of AgrobiotechnologySchool of Life SciencesThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina Shenzhen Research Institute The Chinese University of Hong KongShenzhen 518057China Shenzhen Technology University Shenzhen 518000China School of Chemical Biology&Biotechnology Peking University Shenzhen Graduate SchoolNanshan DistrictShenzhen 518055China

Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the underlying mechanisms still need to be fully characterized. Post-translational regulation of the activities of transcription factors(TFs) is a dynamic and essential mechanism for plant growth and development. CRL3 BPME3 ligase is a CULLIN3-based E3 ligase involved in orchestrating protein stability via the ubiquitin proteasome pathway. Our study shows that the mutation of MYB106 induced early flowering phenotype while over-expression of MYB106 delayed Arabidopsis flowering. Transcriptome analysis of myb106 mutants reveals 257 differentially expressed genes between wild type and myb106-1 mutants, including Flowering Locus T(FT) which is related to flowering time. Moreover, in vitro electrophoretic mobility shift assays(EMSA), in vivo chromatin immunoprecipitation quantitative polymerase chain reaction(ChIP-q PCR) assays and dual luciferase assays demonstrate that MYB106 directly binds to the promoter of FT to suppress its expression. Furthermore, we confirm that MYB106 interacts with BPM proteins which are further identified by CRL3 BPME3 ligases as the substrate. Taken together, we have identified MYB106 as a negative regulator in the control of flowering time and a new substrate for CRL3 BPM E3 ligases in Arabidopsis.

关键词： flowering time MYB transcription factor E3 ligase CUL3~(BPM)

Characterization of Short-chain fatty acid-producing and cholesterol assimilation potential probiotic Lactic acid bacteria from Chinese fermented rice

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Food Bioscience 2023年第2期52卷 646-654页

作者： Nageena Qayyum wang Shuxuan Qin Yantin wang Ruiling shuang wang Mohamedelfatieh Ismael Xin Lu College of Food Science and Engineering Northwest Agriculture and Forestry UniversityYangling712100China

Probiotic strains are phrased as beneficial for life because this bacterial association has substantial effects on human and animal health when administrated orally,among which bio-active metabolite short-chain fatty acids production and cholesterol assimilation are most *** the short-chain fatty acids,mainly acetate,propionate,and butyrate reveal valuable effects on human *** this research,the ability of 9 the potential lactic acid bacteria food-isolated probiotics was investigated to produce bio-active metabolite short-chain fatty acids directly or indirectly,and cholesterol-lowering ability through various *** 16S rRNA gene sequences were ***-chain fatty acids were quantified by gas chromatography,while cholesterol assimilation was measured using the o-phthalaldehyde method with slight *** isolated strains through this study have the ability to optimize cholesterol content in the culture broth,short-chain fatty acid production,and survival in extreme alkaline and acidic *** highest resistance to bile salt concentrations,γ-hemolysis,and strong antibacterial activity against pathogenic indicators,especially Escherichia *** highest Lipid accumulation inhibitory effect was noticed in Lentilactobacillus hilgardii LMG 07934 as a potential probiotic isolate on the basis of cholesterol assimilation capacity(i.e.,91.4%),along with the ability to produce abundant i.e.,butyric acid,propionic acid,and acetic acid,followed by Lacticaseibacillus paracasei ATCC 334 which has 85.3%cholesterol assimilation *** findings demonstrated that these isolated strains from Chinese fermented rice can be used as a potential probiotic and alternate therapeutic supplement because of their ability to produce short-chain fatty acids and their cholesterol-lowering ability.

关键词： Probiotics Cholesterol assimilation Bio-active metabolite

Influences of H^(+)Implantation on the Boron-Doped Synthesized by Chemical Vapor Deposition Diamond Films

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Chinese Physics Letters 2000年第9期17卷 686-688页

作者： wang shuang-Bao ZHU Pei-Ran wang Yu-Guang FENG Ke-An Institute of Physics Chinese Academy of SciencesBeijing 100080

Diamond films(DF)were preliminarily B doped in situ during chemical vapor ***,the films were implanted with 120keV H^(+)to dose of 5×10^(14)~5×10^(16)cm^(-2).After the implantation,the B doped DF become insulating and Raman measurements indicate that the implantation has amorphous carbon and graphite *** is known that the formation of H-B pairs plays an important pole in property ***,for larger dose cases,the electrical resistance of DF is influenced by radiation damage and/or non-diamond *** addition to them,annealing makes the specimens conducting *** phenomenon maybe has potential for application in designing DF device.

关键词： Diamond implantation conducting

Effect of Sn^(4+) B-Site Substitution on the Microstructure and Dielectric Properties of Ba(Mg_(1/3)Ta_(2/3))O_3 Microwave Ceramics

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Journal of Materials Science & Technology 2005年第5期21卷 773-775页

作者： Shunhua WU Guoqing wang, shuang wang and Dandan LIUInstitute of Electronics and Information Engineering, Tianjin University, Tianjin 300072, China Institute of Electronics and Information Engineering Tianjin University Tianjin 300072 China

The effect of Sn^4＋（BaSnO3） B-site substitution on the microstructure and dielectric properties of Ba（Mg1/3Ta2/3）O3 microwave ceramics was investigated. X-ray diffraction shows that a complex perovskite material Ba（Mg1/3Ta2/3）O3 was prepared. As Sn^4＋ content x increases in the （1-x） Ba（Mg1/3Ta2/3）O3-xBaSnO3 （x=0.00～0.20） system, the dielectric constant generally keeps unchanged, while TCF changes from positive to negative. Although the addition of Sn^4＋ reduces the ordering degree, Q f0 is still increased when the ceramics density increases. This trend implies that Q f0 of this system is mostly determined by ceramics density rather than ordering degree. After sintering at 1500℃ for 3 h, the system with x=0.15 was found to have excellent dielectric properties as follows：ε≈25, Q f0≥300,000 GHz at 7 GHz, TCF=-0.6×10^-6/℃.

关键词： Ba(Mg1/3Ta2/3)O3 Complex perovskite Microwave Dielectric properties

Study on Preparation of Nitrogen-Doped Hierarchically Porous and High-Performance Carbon Materials from Seaweed Biomass

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Journal of Renewable Materials 2022年第2期10卷 541-560页

作者： Kaihan Xie Yamin Hu Stephen Afonaa-Mensah Chuan Yuan Bin Cao shuang wang Qian wang School of Energy and Power Engineering Jiangsu UniversityZhenjiangChina Takoradi Technical University TakoradiGhana

Use Enteromorpha clathrate as a carbon and nitrogen precursor could obtain the N-doped porous carbon mate-rials by two-step *** this paper,the exogenous nitrogen(urea,melamine)and activating agents(KOH,ZnCl_(2))were employed for the production of higher-performance carbon materials from seaweed ***,BET,FTIR,XRD,XPS,Raman and ultimate analyses were carried out to investigate the morpho-structural and elemental peculiarities of the carbonaceous ***,cyclic voltammetry(CV),galvanostatic charge-discharge(GCD)and cyclic charge-discharge tests were carried out to examine the electrochemical properties of the *** results showed that electrochemical performance of the carbonaceous materials improved by adding the appropriate nitrogen *** improvement was observed under either condition,when the nitro-gen source and activating agent were melamine and ZnCl_(2) or urea and KOH,***,under the condition with KOH as the activator,the nitrogen content of carbon material without exogenous nitrogen was initially 1.46%.After the addition of urea or melamine,the nitrogen content increased to 4.86%and 6.18%,*** the condition with ZnCl_(2) as the activator,and without exogenous nitrogen,the nitrogen con-tent of carbon materials was initially 3.75%.However,after adding urea or melamine,the nitrogen content increased to 12.11%and 14.76%,*** carbonaceous materials(prepared from urea/KOH and mel-amine/ZnCl_(2))showed excellent gravimetric capacitances of 172 and 151.5 F/g at 1A/***,at a current density of 5 A/g,their specific capacitance retention rate reached 78.3%and 82.6%respectively after 1000 cycles.

关键词： Exogenous nitrogen incorporation N-doped porous carbon two-steps pyrolysis enteromorpha clathrate

Polarity tunable complementary logic circuits

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Science China(Information Sciences) 2023年第12期66卷 291-292页

作者： Chen PAN Jingwen SHI Pengfei wang shuang wang Cong wang Bin CHENG Shi-Jun LIANG Feng MIAO Institute of Interdisciplinary Physical Sciences School of ScienceNanjing University of Science and Technology Institute of Brain-Inspired Intelligence National Laboratory of Solid State MicrostructuresSchool of PhysicsCollaborative Innovation Center of Advanced MicrostructuresNanjing University

The utilization of excellent electrical characteristics of 2D semiconductors has led to a series of revolutionary developments in the research fields of neuromorphic circuits, reconfigurable circuits, and large-area thin-film electronics [1–4].In order to further explore the potential of 2D materials in the development of electronic technology,

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Comparison of nitrogen losses by runoff from two different cultivating patterns in sloping farmland with yellow soil during maize growth in Southwest China

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Journal of Integrative Agriculture 2022年第1期21卷 222-234页

作者： HE Shu-qin MA Rui wang Na-na wang shuang LI Ting-xuan ZHENG Zi-cheng College of Forestry Sichuan Agricultural UniversityChengdu 611130P.R.China College of Resources Science Sichuan Agricultural UniversityChengdu 611130P.R.China

The loss of N in farmland is an important cause of agricultural non-point source pollution, which seriously impacts the aquatic environment. A two-year(2017–2018) experiment was conducted to investigate the characteristics of runoff and N losses under different tillage practices. Taking downslope ridge planting and cross ridge planting as the experimental treatments,the characteristics of surface runoff, interflow, and N losses in sloping farmlands with yellow soil were studied throughout the maize growth period. As the rainfall increased, the surface runoff and interflow also increased. The surface runoff and N losses in the surface runoff of downslope ridge planting were significantly higher than those of cross ridge planting. The interflow volumes and N losses in the 0–20 and 20–40 cm soil layers of the cross ridge planting were significantly higher than those of the downslope ridge planting. The total N(TN) losses from surface runoff accounted for 54.95–81.25% of the N losses from all pathways. Therefore, we inferred that surface runoff is the main pathway of N losses. Dissolved total N(DTN) was the main form of N loss under different tillage measures, as it accounted for 55.82–94.41% of the TN losses,and dissolved organic N accounted for 52.81–87.06% of the DTN losses. Thus, we inferred that dissolved N is the main form of N loss. Future research must focus on the prevention and control of the N losses during the maize seedling stage to reduce the environmental pollution caused by ammonium N through runoff.

关键词： nitrogen losses surface runoff interflow maize growth stages tillage measures

Contact Force Distribution and Static Load-carrying Capacity of Large Size Double Row Four-point Contact Ball Bearing

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Defence Technology（防务技术） 2013年第4期9卷 229-236页

作者： Yan-shuang wang Qian-qian YUAN Henan University of Science and Technology

Clearance not only affects the startup torque,rotation precision and stiffness of bearing,but also affects the load distribution,load-carrying capacity and life of bearing.A computational model in which the clearance of bearing is first included is presented for determining the contact force distribution and static load-carrying capacity of a double row four-point contact ball bearing which is subjected to the combined radial,axial and overturning moment *** relation between the negative axial clearance and the contact force distribution is *** static load-carrying capacity curves are established,and the effects of the changes in negative axial clearance,curvature radius coefficient of raceway groove and initial contact angle on the static load-carrying capacity are *** results show that,with the increase in the absolute value of negative clearance,the maximum contact load decreases first and then *** clearance values in the range of 0.2 mme0 mm have little effect on the static load-carrying capacity of *** the increase in the curvature radius coefficient of raceway groove and the decrease in the initial contact angle,the static load capacity of bearing decreases.

关键词：四点接触球轴承承载能力接触力静态双排大尺寸轴向游隙轴向间隙