Characterization of Short-chain fatty acid-producing and cholesterol assimilation potential probiotic Lactic acid bacteria from Chinese fermented rice

作     者：Nageena Qayyum Wang Shuxuan Qin Yantin Wang Ruiling Shuang Wang Mohamedelfatieh Ismael Xin Lu 

作者机构：College of Food Science and EngineeringNorthwest Agriculture and Forestry UniversityYangling712100China 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2023年第52卷第2期

页      面：646-654页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Study was financially supported by Special Fund for Agro-scientifc Research in the Public Interest[Grant No.201503135] Doctoral Program of Chinese scholarship council(CSC No.2019SLJ018966) 

主　　题：Probiotics Cholesterol assimilation Bio-active metabolite 

摘      要：Probiotic strains are phrased as beneficial for life because this bacterial association has substantial effects on human and animal health when administrated orally,among which bio-active metabolite short-chain fatty acids production and cholesterol assimilation are most *** the short-chain fatty acids,mainly acetate,propionate,and butyrate reveal valuable effects on human *** this research,the ability of 9 the potential lactic acid bacteria food-isolated probiotics was investigated to produce bio-active metabolite short-chain fatty acids directly or indirectly,and cholesterol-lowering ability through various *** 16S rRNA gene sequences were ***-chain fatty acids were quantified by gas chromatography,while cholesterol assimilation was measured using the o-phthalaldehyde method with slight *** isolated strains through this study have the ability to optimize cholesterol content in the culture broth,short-chain fatty acid production,and survival in extreme alkaline and acidic *** highest resistance to bile salt concentrations,γ-hemolysis,and strong antibacterial activity against pathogenic indicators,especially Escherichia *** highest Lipid accumulation inhibitory effect was noticed in Lentilactobacillus hilgardii LMG 07934 as a potential probiotic isolate on the basis of cholesterol assimilation capacity(i.e.,91.4%),along with the ability to produce abundant i.e.,butyric acid,propionic acid,and acetic acid,followed by Lacticaseibacillus paracasei ATCC 334 which has 85.3%cholesterol assimilation *** findings demonstrated that these isolated strains from Chinese fermented rice can be used as a potential probiotic and alternate therapeutic supplement because of their ability to produce short-chain fatty acids and their cholesterol-lowering ability.