检索结果-南通市图书馆

Optimization of Solid-State Fermentation with Lactobacillus brevis and Aspergillus oryzae for Trypsin Inhibitor Degradation in Soybean Meal

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2013年第5期12卷 869-876页

作者： GAO You-ling wang cai-sheng ZHU Qiu-hua QIAN Guo-ying Laboratory of Aquatic Molecular Nutrition and Physiology College of Biological and Environmental SciencesZhejiang Wanli Univerisity

The aim of the present study was to optimize trypsin inhibitor degradation in soybean meal by solid-state fermentation （SSF） with Lactobacillus brevis and Aspergillus oryzae, and to determine the effect of SSF on phytic acid, crude protein, crude fat, and amino acid profile. Response surface methodology （RSM） with Box-Behnken design was used to optimize SSF. The optimal conditions derived from RSM for L. brevis fermentation were： pH=5. 1; inoculum size=10%; duration=72 h; substrate to water ratio=1.5. The minimum content of trypsin inhibitors was 6.4 mg g^-1 dry matter. The optimal conditions derived from RSM for A. oryzae fermentation were： substrate to water ratio= 0.8 1; inoculum size=4%; duration=120 h. The minimum content of trypsin inhibitors was 1.6 mg g^-1 dry matter. Both L. brevis and A. oryzae decreased trypsin inhibitors dramatically （57.1 and 89.2% respectively）. L. brevis fermentation did not affect phytic acid （0.4%） and crude fat （5.2%） considerably, whereas A. oryzae fermentation degraded phytic acid （34.8%） and crude fat （22.0%） contents to a certain extent. Crude protein content was increased after both fermentation （6.4 and 12.9% for L. brevis and A. oryzae respectively）. Urease activity was reduced greatly （83.3 and 58.3% for L. brevis and A. oryzae respectively）. In conclusion, SSF with A. oryzae and L. brevis reduced trypsin inhibitor content and modified major macronutrients in soybean meal.

关键词： Aspergillus oryzae Lactobacillus brevis response surface methodology solid-state fermentation soybean meal trypsin inhibitors

Transcriptomic profile of human erythroleukemia cells in response to Sargassum fusiforme polysaccharide and its structure analysis

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2021年第10期19卷 784-795页

作者： DING Hao-Miao FU Rui-Jie XIE Ce wang cai-sheng QIAN Guo-Ying College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingbo 315100China School of Marine Sciences Ningbo UniversityNingbo 315211China

Sargassum fusiforme(***)has been used as an ingredient in Chinese herbal medicine for thousands of ***,there are a limited number of studies concerning its therapeutic *** performance gel permeation chromatography(HPGPC)analysis showed that the average molecular weight of the *** polysaccharide,SFPS 191212,is 43 *** 191212 is composed of mannose,rhamnose,galactose,xylose,glucose,and fucose(at a molar ratio:2.1:2.9:1.8:15.5:4.6:62.5)withα-andβ-*** present research evaluated the anti-tumor potential of the *** polysaccharide in human erythroleukemia(HEL)cells in *** explore the SFPS 191212’s apoptosis mechanism in HEL cells,transcriptome analysis was performed on HEL cells that were incubated with SFPS *** inhibitory effect of SFPS 191212 on HEL cell growth was also *** was found that SFPS 191212 inhibited HEL cell proliferation,reduced cell viability in a concentration-dependent manner,and induced an insignificant toxic effect on normal human embryonic lung(MRC-5)*** with the control group,transcriptome analysis identified a total of 598 differentially expressed genes(DEGs),including 243 up-regulated genes and 355 downregulated *** Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on all DEGs,and 900 GO terms and 52 pathways were found to be significantly ***,23 DEGs were randomly selected and confirmed by quantitative real-time polymerase chain reaction(qRT-PCR).Moreover,SFPS 191212 down-regulated the PI3K/Akt signal transduction *** results provide a framework for understanding the effect of SFPS 191212 on cancer cells and can serve as a resource for delineating the anti-tumor mechanisms of ***.

关键词： Human erythroleukemia Polysaccharide Sargassum fusiforme Transcriptome

Effect of Sargassum fusiforme polysaccharide on apoptosis and its possible mechanism in human erythroleukemia cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2020年第10期18卷 749-759页

作者： DING Hao-Miao CHEN Xue-Jia CHEN Hai-Min wang cai-sheng QIAN Guo-Ying School of Marine Sciences Ningbo UniversityNingbo 315211China College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingbo 315100China

This study aimed to investigate the effects of Sargassum fusiforme polysaccharide(SFPS I,II,and III)on the apoptosis and regulation of human erythroleukemia(HEL)*** effect of different doses of SFPS on HEL cell growth was detected using the Cell Counting Kit-8 method,and apoptosis was detected by Hoechst *** cycle distribution and apoptosis were detected using flow *** of the cell cycle gene,p53,antiapoptotic genes,Bcl-xL and Bcl-2,and pro-apoptotic genes,Bax,Bad,and Caspase-3,as well as the expression of the corresponding proteins,were detected using real-time quantitative polymerase chain reaction(qPCR)and Western *** results showed that SFPS Ⅱ and Ⅲ decreased HEL cell viability and induced HEL cell *** concentrations of SFPS(Ⅰ,Ⅱ,and Ⅲ)were detected that induced much less toxic effect in normal human embryonic lung(MRC-5)cells,and SFPS Ⅰ increased cell proliferation,indicating its favorable selectivity towards cancer *** mechanism by which SFPS induced apoptosis was also found to be related to the induction of cell cycle arrest in the G0/G1 phase and the increased expression of apoptosis-related genes and *** concluded that SFPS induces HEL cell apoptosis,possibly via activation of the Caspase pathway,providing the theoretical basis for the development of SFPS-based anti-tumor drug products.

关键词： Sargassum fusiforme Polysaccharides Human erythroleukemia(HEL cells) Apoptosis Cell cycle

机械密封O形橡胶密封圈力学行为的有限元分析

在线全文

学校读者我要写书评

暂无评论

华东理工大学学报（自然科学版） 2013年第6期39卷 761-767页

作者：王财生秦瑶安琦华东理工大学机械与动力工程学院上海200237

利用有限元软件ANSYS建立了机械密封O形橡胶密封圈轴对称计算模型,分析计算了在两种不同的安装方式下的变形、Von Mises应力分布及其与轴套接触的接触应力和摩擦力,研究了O形密封圈的预紧压缩率、介质压力和凹槽宽度对密封性能的影响。... 详细信息

利用有限元软件ANSYS建立了机械密封O形橡胶密封圈轴对称计算模型,分析计算了在两种不同的安装方式下的变形、Von Mises应力分布及其与轴套接触的接触应力和摩擦力,研究了O形密封圈的预紧压缩率、介质压力和凹槽宽度对密封性能的影响。研究表明:O形密封圈的变形、Von Mises应力、主接触面接触应力及其与轴套的摩擦力均随介质压力和截面压缩率的增大而增大,并随着凹槽宽度的减小而增大;凹槽宽度的设计不能过小,两种不同的安装方式下选用O形密封圈的压缩量应有所不同。

关键词：机械密封 O形密封圈有限元

亚慢性染氟对雌性小鼠雌激素受体mRNA表达的影响

在线全文

学校读者我要写书评

暂无评论

中华地方病学杂志 2013年第2期32卷 146-148页

作者：王彩生唐勇王春江苏省疾病预防控制中心环境疾病(地方病)防治所南京210009 江苏省淮阴区疾病预防控制中心南通大学公共卫生学院

目的观察亚慢性染氟（氟化钠）对雌性小鼠雌激素受体（ER）mRNA表达的影响。方法雌性小鼠40只，按体质量随机分成4组：对照组、低氟组、中氟组、高氟组，每组10只。分别饮用0．1、5、25mg／L氟化钠溶液，喂养12周后。RT-PCR法检测小鼠... 详细信息

目的观察亚慢性染氟（氟化钠）对雌性小鼠雌激素受体（ER）mRNA表达的影响。方法雌性小鼠40只，按体质量随机分成4组：对照组、低氟组、中氟组、高氟组，每组10只。分别饮用0．1、5、25mg／L氟化钠溶液，喂养12周后。RT-PCR法检测小鼠卵巢中ERa、ERl3mRNA表达。结果对照组、低氟组、中氟组、高氟组小鼠卵巢组织ERa、ERl3mRNA表达分别为0．7028±0．0474、0．7195．4±0．0552、0．6479±0．0590、0．5684±0．0513。0．8418±0．0131、0．7729±0．0974、0．7610±0．0984、0．8026±0．0234。其中高氟组小鼠卵巢组织ERamRNA表达与对照组比较，差异有统计学意义（P〈0．05）。结论亚慢性染氟可导致卵巢组织中雌激素受体表达下降，后者对雌性小鼠的生殖发育可能有一定的影响作用。

关键词：氟化钠小鼠雌激素受体

Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng Li Meifeng cai shengjun Miao Yuan Li Yu wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第1期17卷 281-292页

作者： Xing wang Wei Pi sheng Hu Haifeng Bao Na Yao Wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

在线全文

学校读者我要写书评

暂无评论

V型填料密封压紧过程受力计算方法

华东理工大学学报（自然科学版） 2013年第1期39卷 108-114页

作者：秦瑶王财生周琼安琦华东理工大学机械与动力工程学院上海200237

以V型填料密封为研究对象,对其填料装填压紧过程进行力学分析,运用弹性力学的有关理论,在考虑摩擦力、压紧力和内外接触过盈量的前提下,建立了填料装填过程的受力和变形的计算模型。运用所建立的填料密封力学计算模型,针对一个具体的V... 详细信息

以V型填料密封为研究对象,对其填料装填压紧过程进行力学分析,运用弹性力学的有关理论,在考虑摩擦力、压紧力和内外接触过盈量的前提下,建立了填料装填过程的受力和变形的计算模型。运用所建立的填料密封力学计算模型,针对一个具体的V型填料密封进行了计算研究,在几种不同的工况下,计算得到了装填过程压紧力和内外过盈量大小对轴向位移和内外接触面上的应力分布规律的影响曲线,并对这些曲线进行了分析说明。

关键词： V型密封圈力学模型压紧力过盈应力

在线全文

学校读者我要写书评

暂无评论

密封环滑动面变形对润滑性能的影响

华东理工大学学报（自然科学版） 2013年第2期39卷 228-234页

作者：王财生周琼秦瑶安琦华东理工大学机械与动力工程学院上海200237

建立了机械密封环滑动面变形的几何模型,采用自适应相关函数模拟密封环滑动面的微观表面形貌。结合所模拟的粗糙表面,建立了滑动面变形时的微观流体动压润滑模型,采用有限差分法,运用MATLAB语言进行编程求解,实现了对一定外载荷下滑动... 详细信息

建立了机械密封环滑动面变形的几何模型,采用自适应相关函数模拟密封环滑动面的微观表面形貌。结合所模拟的粗糙表面,建立了滑动面变形时的微观流体动压润滑模型,采用有限差分法,运用MATLAB语言进行编程求解,实现了对一定外载荷下滑动面微观流体润滑压力分布和流体膜厚度分布的计算。在此基础上,通过一个具体的实例计算分析,系统研究了滑动面变形和外载荷变化对密封环流体膜厚、摩擦力、发热量、泄漏量等润滑性能的影响规律,得到一系列规律性曲线,并进行了一定的分析说明。

关键词：密封环变形粗糙度动压润滑数值模拟