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Effects of Limiting Amino Acids on Rumen Fermentation and Microbial Community In vitro

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Agricultural Sciences in China 2008年第12期7卷 1524-1531页

作者： wang Meng-zhi wang Hong-rong CAO Heng-chun LI Guo-xiang ZHANG jie College of Animal Science and Technology Yangzhou University Yangzhou 225009 P.R.China

Three goats fitted with cannula were used to provide rumen liquor to investigate the effects of limiting amino acids on rumen fermentation and microbial community in vitro. The removal method was used in the current experiments. Treatments are total essential amino acid （TEAA）, His-removal, Lys-removal, Met-removal, and branch chain amino acid （BCAA）- removal. Results indicated that, pH-value ranged between 5.9 and 6.8, with the highest mean value for the group with BCAA-removal （6.54） in the culture. Concentration of NH3-N ranged between 10.99 to 30.51 mg 100 mL^-1, with the group of TEAA recording the highest average NH3-N concentration （17.85 mg 100 mL^-1）. Yields of microbial protein and limiting degree on microbial growth varied with treatments （P 〈 0.01）, and the lowest accrued in treatment with BCAA-removal （0.1389, 0.1772, and 0.3161 mg mL^-1 for bacteria, protozoa, and mixed microbes, respectively）, compared to the group with TEAA, microbial production of mixed microbes decreased by 44.52%. As for micro-flora, protozoa to bacteria ratio was the lowest for the group with Lys-removal （89.12%）, while the highest for the group with BCAA-removal （127.60%）（P 〈 0.01）. Furthermore, PCR-SSCP analysis revealed that, microbial profile subjected to substrates within bacteria and protozoa groups. It was therefore concluded that, dietary amino acid influenced both rumen fermentation and microbial characteristics.

关键词： limiting amino acids rumen microbes limiting degree,

Hydrothermal Synthesis,Structure and Characterization of a New 2D Co(Ⅱ) Coordination Polymer Based on Flexible Double Imidazole Derivative and Oxalate

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Chemical Research in Chinese Universities 2012年第6期28卷 942-946页

作者： LIU Lian-li HUANG Jing-jing wang Xiu-li LIU Guo-cheng YANG Song MU bao Silicon Materials Engineering Technology Research Centre of Liaoning Province Department of Chemistry Bohai University Jinzhou 121000 P. R. China

A 1D supramolecular compound [dmbbbi]（1） and a 2D cobalt coordination polymer [Co（dmbbbi）（ox）] 2（2） [dmbbbi=1,1-（1,4-butanediyl）bis（5,6-dimethylbenzimidazole）,ox=oxalate],C48H52Co2N8O8,were obtained under hydrothermal conditions by tuning the molar ratio of the *** crystal structure analysis reveals that in compound 1,the adjacent dmbbbi molecules connect with each other via hydrogen bonds to form a 1D supramolecular *** compound 2,two crystallographically independent Co2＋ ions show the same six-coordination *** Co2＋ ion is coordinated by four oxygen atoms from two ox anions and two nitrogen atoms from two cis-dmbbbi *** adjacent Co2＋ ions are bridged by ox anions to generate an infinite 1D zigzag chain,which is extended by pairs of dmbbbi ligands to form a 2D honeycomb-like（6,3） ***,the thermal stability and the electrochemical property of compound 2 were studied.

关键词： Hydrothermal synthesis Crystal structure Molar ratio Double imidazole derivative

M2 macrophage-related molecular subtypes and prognostic index for prostate cancer patients through integrating single-cell and bulk RNA sequencing analysis

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Genes & Diseases 2024年第4期11卷 131-133页

作者： Dechao Feng Xu Shi Dengxiong Li Ruicheng Wu jie wang Wuran Wei Ping Han Department of Urology Institute of UrologyWest China HospitalSichuan UniversityChengduSichuan 610041China

Prostate cancer(PCa)is considered as an age-related disease and accounts for the most prevalence of urinary malignancies in men.1 We previously analyzed the cellular landscape of tumor microenvironment in PCa patients,where we found that cancer-related fibroblasts might play an important role in tumorigenesis and the development of PCa and tumor-associated macrophage(TAM)could function synergistically with them.2 Thus,we further integrated single-cell and bulk RNA sequencing with meta-analysis to specify the prognostic effect of TAM on PCa and construct molecular subgroups and predictive index to guide clinical practice.

关键词： patients macrophage cancer

Clinical and immunological features of convalescent pediatric patients infected with the SARS-CoV-2 Omicron variant in Tianjin, China

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Virologica Sinica 2022年第6期37卷 850-859页

作者： Jing-Yu wang Tian-Ning Li Chun-Lei Zhou jie Zhao Meng wang Yuan wang Yan Jiang He-Nan Dong Qian-Ru Qi Hong Mu Department of Clinical Lab Tianjin First Central HospitalTianjin300192China The First Central Clinical College Tianjin Medical UniversityTianjin300192China Department of Clinical Lab Tianjin Children's HospitalTianjin300074China

COVID-19 has spread surprisingly fast worldwide, and new variants continue to emerge. Recently, the World Health Organization acknowledged a new mutant strain “Omicron”, with children were accounting for a growing share of COVID-19 cases compared with other mutant strains. However, the clinical and immunological characteristics of convalescent pediatric patients after Omicron infection were lacking. In this study, we comparatively analyzed the clinical data from pediatric patients with adult patients or healthy children and the effects of SARSCoV-2 vaccine on the clinical and immune characteristics in convalescent pediatric patients. Our results indicated that convalescent pediatric patients had unique clinical and immune characteristics different from those of adult patients or healthy children, and SARS-CoV-2 vaccination significantly affected on the clinical and immune characteristics and the prevention of nucleic acid re-detectable positive(RP) in convalescent patients. Our study further deepens the understanding of the impact of Omicron on the long-term health of pediatric patients and provides a valuable reference for the prevention and treatment of children infected with Omicron.

关键词： COVID-19 SARS-CoV-2 Omicron variant Clinical characteristics Pediatric patients SARS-CoV-2 vaccine

Pyrrolizidine alkaloids:An update on their metabolism and hepatotoxicity mechanism

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Liver Research 2019年第3期3卷 176-184页

作者： jie Xu Weiqian wang Xiao Yang Aizhen Xiong Li Yang Zhengtao wang The Ministry of Education Key Laboratory for Standardization of Chinese Medicines and the State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Interdisciplinary Integrative Medicine Research Shanghai University of Traditional Chinese MedicinesShanghaiChina

Pyrrolizidine alkaloids(PAs)are among the most hepatotoxic natural compounds that are widely distributed throughout the *** PAs are metabolically activated to trigger *** to herbal medicine containing PAs and food supplements contaminated by PAs is considered to be one of the two main causes of hepatic sinusoidal obstruction syndrome(HSOS),which is a rare hepatic vascular disease with a high mortality ***-induced HSOS cases have been reported ***,there is no clinically effective therapy for PAs-induced HSOS,which is partially because the toxic mechanism is not fully *** review focuses on updating the information on the metabolism and the molecular mechanisms of PAs hepatotoxicity,including oxidative stress,apoptosis,and dysfunction of bile acid metabolism,and their interactions.

关键词： Pyrrolizidine alkaloids(PAs) Hepatic sinusoidal obstruction syndrome (HSOS) Metabolism Toxicity mechanism Oxidative stress Apoptosis Dysfunction of bile acid metabolism

Calcium phosphate cements for bone engineering and their biological properties

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Bone Research 2017年第4期5卷 286-304页

作者： Hockin HK Xu Ping wang Lin wang Chongyun bao Qianming Chen Michael D Weir Laurence C Chow Liang Zhao Xuedong Zhou Mark A Reynolds Department of Endodontics Periodontics and ProsthodonticsUniversity of Maryland School of DentistryBaltimoreMD 21201USA Center for Stem Cell Biology and Regenerative Medicine University of Maryland School of MedicineBaltimoreMD 21201USA University of Maryland Mariene and Stewart Greenebaum Cancer Center University of Maryland School of MedicineBaltimoreMD 21201USA Mechanical Engineering Department University of Maryland Baltimore CountyBaltimoreMD 21250USA State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengduSichuan 610041China VIP Integrated Department Stomatological Hospital of Jilin UniversityChangchunJilin 130011China Volpe Research Center American Dental Association FoundationNational Institute of Standards&TechnologyGaithersburgMD 20899USA Department of Orthopaedic Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515China

Calcium phosphate cements （CPCs） are frequently used to repair bone defects. Since their discovery in the 1980s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. Much effort has been made to enhance the biological performance of CPCs, including their biocompatibility, osteoconductivity, osteoinductivity, biodegradability, bioactivity, and interactions with cells. This review article focuses on the major recent developments in CPCs, including 3D printing, injectability, stem cell delivery, growth factor and drug delivery, and pre- vascularization of CPC scaffolds via co-culture and tri-culture techniques to enhance angiogenesis and osteogenesis.

关键词： CPC BMSCs Calcium phosphate cements for bone engineering and their biological properties

Intra-corporeal delta-shaped anastomosis in laparoscopic right hemicolectomy for right colon cancer:a safe and effective technique

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Gastroenterology Report 2019年第4期7卷 272-278,I0002页

作者： Hao Su Wei-Sen Jin Peng wang Mandula bao Xue-Wei wang Qian Liu Xi-Shan wang Zhi-Xiang Zhou Hai-Tao Zhou Department of Colorectal Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100021P.R.China Department of Anorectal Diseases General Hospital of Chinese Armed Police ForcesNo.69 Yongding RoadHaidian DistrictBeijing100039P.R.China

Background and objective:Intra-corporeal delta-shaped anastomosis(IDA)is an important development in laparoscopic digestive-tract *** applied it in laparoscopic right hemicolectomy for right colon cancer and compared the short-term outcomes between the patients treated with IDA and conventional extracorporeal anastomosis(EA).Methods:Between 1 January 2016 and 1 October 2017,36 and 50 patients who underwent IDA and EA,respectively,were *** on clinicopathological characteristics,surgical outcomes,post-operative recovery and complications were collected and compared between the two ***:Surgical outcomes and clinicopathological characteristics were similar between the two groups except the length of incision,which was significantly shorter in the IDA group than in the EA group(4.660.6 vs 5.660.7 cm,P<0.001).The time to ground activities,fluid diet intake and post-operative hospitalization did not differ between the groups;however,the time to first flatus was significantly shorter in the IDA group than in the EA group(2.860.5 vs 3.260.8 days,P=0.004).The post-operative visual analogue scale for pain was lower in the IDA group than in the EA group on post-operative Day 1(4.060.7 vs 4.561.0,P=0.002)and post-operative Day 3(2.760.6 vs 3.460.6,P<0.001).The surgical complication rates were 8.3 and 16.0%in the IDA and EA groups(P=0.470),*** complications such as anastomotic bleeding,stenosis and leakage occurred in any ***:IDA is safe and feasible and shows more satisfactory short-term outcomes than EA.

关键词： Colon cancer delta-shaped anastomosis intra-corporeal anastomosis digestive-tract reconstruction laparoscopic right hemicolectomy

Hierarchical coupling effect in hollow Ni/NiFe2O4-CNTs microsphere via spray-drying for enhanced oxygen evolution electrocatalysis

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Nano Research 2020年第2期13卷 437-446页

作者： Xuefeng Yu Guanyu Chen Yizhe wang Jiwei Liu Ke Pei Yunhao Zhao Wenbin You Lei wang jie Zhang Linshen Xing Jingjun Ding Guangzhou Ding Min wang Renchao Che Laboratory of Advanced Materials Department of Materials Science and Collaborative Innovation Center of Chemistry for Energy Materials(iChem)Fudan UniversityShanghai 200438China Materials Genome Institute International Centre of Quantum and Molecular Structuresand Physics DepartmentShanghai UniversityShanghai 200444China College of Materials and Environmental Engineering Hangzhou Dianzi UniversityHangzhou 310012China

Design and fabrication of cost-effective transition metal and their oxides-based nanocomposites are of paramount significance for metal-air batteries and ***,the traditional optimized designs for nanostructure are complicated,low-efficient and underperform for wide-scale ***,a novel hierarchical framework of hollow Ni/NiFe2O4-CNTs compositemicrosphere forcibly-assembled by zero-dimensional(OD)Ni/NiFo204 nanoparticle(<16 nm)and one-dimensional(1D)self-supporting CNTs was fabricated *** from the unique nanostructure,such monohybrids can achieve remarkable oxygen evolution reaction(OER)performance in alkaline media with a low overpotential and superior durability,which exceeds most of the commercial catalysts based on IrO/RuO2 or other non-noble metal *** enhanced OER performance of Ni/NiFe2OA-CNTs composite is mainly ascribed to the increased catalytic activity and the optimized conductivity induced by the effects of strong hierarchical coupling and charge transfers between CNTs and Ni/NiFe204 *** effects are greatly boosted by the polarized heterojunction interfaces confirmed by electron *** density functional theory(DFT)calculation indicates the epitaxial Ni further enriches the intrinsic electrons contents of NiFe204 and thus accelerates absorption/desorption kinetics of OER *** work hereby paves a facile route to construct the hollow composite microsphere with excellent OER electrocatalytic activity based on non-noble metal oxide/CNTs.

关键词： spray-drying novel structure oxygen evolution reaction(OER) electron holography composite microsphere

Wolbachia-induced expression of kenny gene in testes affects male fertility in Drosophila melanogaster

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Insect Science 2020年第5期27卷 869-882页

作者： John C.Biwot Hua-bao Zhang Chen Liu Jun-Xue Qiao Xiao-Qiang Yu Yu-Feng wang School of Life Sciences Hubei Key Laboratory of Genetic Regulation and Integrative BiologyCentral China Normal UniversityWuhanChina

Wolbachia are Gram-negative endosymbionts that are known to cause embry-onic lethality when infected male insects mate with uninfected females or with females carrying a different strain of Wolbachia,a situation characterized as cytoplasmice incom-patibility(CD).However,the mechanism of CI is not yet fully understood,although re-cent studies on Drosophila melanogaster have achieved great ***,we found that Wolbachia infection caused changes in the expressions of several immunity-related genes,including significant upregulation of kenny(key),in the testes of *** of key in fly testes led to a significant decrease in egg hatch rates when these fies mate with wild-type ***-infected females could rescue this embryonic ***,in key overexpressing testes terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling signal was significantly stronger than in the control testes,and the level of reactive oxygen species was significantly *** of key also resulted in alterations of some other immunity-related gene expressions,including the downregulation of *** of Zn72D in fly testes also led to a significant decrease in egg hatch *** results suggest that Wolbachia might induce the defect in male host fertility by immunity-related pathways and thus cause an oxidative damage and cell death in male testes.

关键词： Drosophila melanogaster key testes Wolbachia Zn72D