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Science china Chemistry 2007年第4期50卷 468-475页

作者： wang zhengwu1 & yi xizhang2 1 department of food sciences & technology, school of agriculture and biology, shanghai jiao tong university, shanghai 200240, china 2 Institute of Theoretical Chemistry, Shandong university, Jinan 250100, china 1. department of food sciences & technology School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China 2. Institute of Theoretical Chemistry Shandong University Jinan 250100 China

By using the binary anionic/cationic surfactants system CH3(CH2)nOSO_3/CH3(CH2)nN+(CH3)3 as an ex-ample, the molecular exchanging energy (ε) of adsorption on the surface monolayer of aqueous solu-tion has been studied. ε can be obtained with two methods. One is from the relationship between ε and the molecule interaction parameter (β). This relationship is founded by considering that the adsorption of mixed surfactants on the surface monolayer of solution satisfies the dimensional crystal model condition under which β can be obtained by testing the surface tension of solution. The other is directly from the molecular structure of surfactants with the Lennard-Jones formula. The results for the studied system show that these two methods coincide well.

关键词： surfactant molecule exchanging energy dimensional crystal model

Emerging contaminants:A One Health perspective

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The Innovation 2024年第4期5卷 140-170,139页

作者： Fang wang Leilei Xiang Kelvin Sze-yin Leung Martin Elsner ying Zhang Yuming Guo Bo Pan Hongwen Sun Taicheng An Guangguo ying Bryan WBrooks Deyi Hou Damian EHelbling Jianqiang Sun Hao Qiu Timothy MVogel Wei Zhang Yanzheng Gao Myrna JSimpson yi Luo Scott XChang Guanyong Su Bryan MWong Tzung-May Fu Dong Zhu Karl JJobst Chengjun Ge Frederic Coulon Jean Damascene Harindintwali Xiankui Zeng Haijun wang Yuhao Fu Zhong Wei Rainer Lohmann Changer Chen Yang Song Concepcion Sanchez-Cid Yu wang Ali El-Naggar yiming Yao Yanran Huang Japhet Cheuk-Fung Law Chenggang Gu Huizhong Shen Yanpeng Gao Chao Qin Hao Li tong Zhang Natàlia Corcoll Min Liu Daniel SAlessi Hui Li Kristian KBrandt Yolanda Pico Cheng Gu Jianhua Guo Jianqiang Su Philippe Corvini Mao Ye Teresa Rocha-Santos Huan He yi Yang Meiping tong Weina Zhang Fidèle Suanon Ferdi Brahushi Zhenyu wang Syed AHashsham Marko Virta Qingbin Yuan Gaofei Jiang Louis A.Tremblay Qingwei Bu Jichun Wu Willie Peijnenburg Edward Topp Xinde Cao Xin Jiang Minghui Zheng Taolin Zhang Yongming Luo Lizhong Zhu Xiangdong Li DamiàBarceló Jianmin Chen Baoshan Xing Wulf Amelung Zongwei Cai Ravi Naidu Qirong Shen Janusz Pawliszyn Yong-guan Zhu Andreas Schaeffer Matthias C.Rillig Fengchang Wu Gang Yu James M.Tiedje State Key Laboratory of Soil and Sustainable agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China university of Chinese Academy of sciences Beijing 100049China department of Chemistry Hong Kong Baptist UniversityHong KongChina HKBU Institute of Research and Continuing Education Shenzhen Virtual University ParkShenzhenChina Technical university of Munich TUM School of Natural SciencesInstitute of Hydrochemistry85748 GarchingGermany school of Resources&Environment Northeast Agricultural UniversityHarbin 150030China Climate Air Quality Research UnitSchool of Public Health and Preventive MedicineMonash UniversityMelbourneVICAustralia Faculty of Environmental Science&Engineering Kunming University of Science&TechnologyKunming 650500China Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300350China Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China Ministry of Education Key Laboratory of Environmental Theoretical Chemistry South China Normal UniversityGuangzhouGuangdong 510006China department of Environmental Science Baylor UniversityWacoTXUSA Center for Reservoir and Aquatic Systems Research(CRASR) Baylor UniversityWacoTXUSA school of Environment Tsinghua UniversityBeijing 100084China school of Civil and Environmental Engineering Cornell UniversityIthacaNY 14853USA Key Laboratory of Microbial technology for Industrial Pollution Control of Zhejiang Province Zhejiang University of TechnologyHangzhou 310014China school of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Laboratoire d’Ecologie Microbienne Universite Claude Bernard Lyon 1UMR CNRS 5557UMR INRAE 1418VetAgro Sup69622 VilleurbanneFrance department of Pla

Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary *** global efforts to mitigate legacy pollutants,the continuous introduction of new substances remains a major threat to both people and the *** response,global initiatives are focusing on risk assessment and regulation of emerging contaminants,as demonstrated by the ongoing efforts to establish the UN’s Intergovernmental Science-Policy Panel on Chemicals,Waste,and Pollution *** review identifies the sources and impacts of emerging contaminants on planetary health,emphasizing the importance of adopting a One Health *** for monitoring and addressing these pollutants are discussed,underscoring the need for robust and socially equitable environmental policies at both regional and international *** actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.

关键词： pollution planet contaminants

水体和滤食性贝类传播新型冠状病毒的研究进展

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浙江大学学报（农业与生命科学版） 2024年第4期50卷 584-590页

作者：吕陈昂王大鹏上海交通大学农业与生物学院食品科学与工程系上海200240

严重急性呼吸综合征冠状病毒2型(severe acute respiratory syndrome coronavirus 2, SARS-CoV-2)即新型冠状病毒,依然严重威胁着高龄或免疫力受损等脆弱人群的健康与生命安全。明确并切断SARS-CoV-2的各种传播途径是科学防控疫情、保... 详细信息

严重急性呼吸综合征冠状病毒2型(severe acute respiratory syndrome coronavirus 2, SARS-CoV-2)即新型冠状病毒,依然严重威胁着高龄或免疫力受损等脆弱人群的健康与生命安全。明确并切断SARS-CoV-2的各种传播途径是科学防控疫情、保护脆弱人群的关键手段。除呼吸道传播,SARS-CoV-2能否通过水或食物进行“粪-口”途径传播尚无定论。滤食性贝类可富集水体中的多种病原,是食源性病毒的重要传播载体。但是,滤食性贝类是否也能富集并传播SARS-CoV-2尚有争议。本文对新型冠状病毒感染患者粪便中的感染性SARS-CoV-2,通过污染水体,富集于滤食性贝类,最终感染人体消化道这一潜在传播途径的相关研究进行系统梳理,着重总结了感染性SARS-CoV-2在天然水体中的衰减规律以及滤食性贝类富集SARS-CoV-2的风险。现有研究结果表明,SARS-CoV-2存在经“粪-口”途径感染人群的风险,人们直接接触患者的粪便有感染风险;然而,在具有完善污水处理系统的地区,人们经接触水体或食用栖息于其中的滤食性贝类而感染SARS-CoV-2的风险非常低。

关键词：严重急性呼吸综合征冠状病毒2型 “粪-口”传播水体滤食性贝类

Boosting wheat functional genomics via an indexed EMS mutant library of KN9204

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Plant Communications 2023年第4期4卷 58-76页

作者： Dongzhi wang Yongpeng Li Haojie wang Yongxin Xu yiman Yang Yuxin Zhou Zhongxu Chen Yuqing Zhou Lixuan Gui yi Guo Chunjiang Zhou Wenqiang Tang Shuzhi Zheng Lei wang Xiulin Guo yingjun Zhang Fa Cui Xuelei Lin Yuling jiao Yuehui He Junming Li Fei He Xigang Liu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Ministry of Education Key Laboratory of Molecular and Cellular biology Hebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityHebei Collaboration Innovation Center for Cell SignalingShijiazhuang 050024China Center for Agricultural Resources Research Institute of Genetics and Development BiologyChinese Academy of SciencesShijiazhuang 050022China university of Chinese Academy of sciences Beijing 100049China department of Life Science TcuniIncChengdu 610000China State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life SciencesCenter for Quantitative BiologyPeking UniversityBeijing 100871China State Key Laboratory of Protein and Plant Gene Research School of Advanced Agricultural SciencesPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Plant Genetic Engineering Center of Hebei Province Institute of Biotechnology and Food ScienceHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China Institute ofCereal and OilCrops Hebei Academy of Agriculture and Forestry SciencesLaboratory of CropGenetics andBreeding ofHebeiShijiazhuang 050035China Key Laboratory of Molecular Module-Based Breeding of High yield and Abiotic Resistant Plants in Universities of Shandong College of AgricultureLudong UniversityYantai 264025China Centre of Excellence for Plant and Microbial Science(CEPAMS) JIC-CASBeijing 100101China

A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental ***,the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for *** this study,we created a library for KN9204,a popular wheat variety in northern china,with a reference genome,transcriptome,and epigenome of different tissues,using ethyl methyl sulfonate(EMS)*** library contains a vast developmental diversity of critical tissues and transition *** capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79%of coding genes had mutations,and each line had an average of 1383 EMS-type *** identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1,Q,TaTB1,and *** tested 100 lines with severemutations in 80 NAC transcription factors(TFs)under drought and salinity stress and identified 13 lines with altered *** analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress,including SNAC1,DREB2B,CML16,and ZFP182,factors known to respond to abiotic ***,we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.

关键词： wheat exome capture sequencing EMS mutagenesis functional genomics

Chromosome-scale genome assembly of Prunus pusilliflora provides novel insights into genome evolution, disease resistance, and dormancy release in Cerasus L.

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Horticulture Research 2023年第5期10卷 220-235页

作者： Songtao Jiu Baozheng Chen Xiao Dong Zhengxin Lv Yuxuan wang Chunjin yin Yan Xu Sen Zhang Jijun Zhu Jiyuan wang Xunju Liu Wanxia Sun Guoqian Yang Meng Li Shufeng Li Zhuo Zhang Ruie Liu Lei wang Muhammad Aamir Manzoor Quero-García José Shiping wang Yahui Lei Ling Yang Elisabeth Dirlewanger Yang Dong Caixi Zhang department of Plant Science School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai200240P.R.China College of food Science and technology Yunnan Agricultural UniversityKunmingYunnan Province650201P.R.China Dali Bai Autonomous Prefecture Academy of Agricultural sciences and Extension DaliYunnan Province671600P.R.China shanghai Botanical Garden Shanghai200231P.R.China College of biology and the Environment Nanjing Forestry UniversityNanjingJiangsu Province200037P.R.China INRAe UMR 1332 de Biologie du Fruit et Pathologie33140 Villenave d’OrnonFrance

Prunus pusilliflora is a wild cherry germplasm resource distributed mainly in Southwest *** its ornamental and economic value,a high-quality assembled *** genome is unavailable,hindering our understanding of its genetic background,population diversity,and evolutionary ***,we de novo assembled a chromosome-scale *** genome using Oxford Nanopore,Illumina,and chromosome conformation capture *** assembled genome size was 309.62 Mb,with 76 scaffolds anchored to eight *** predicted 33035 protein-coding genes,functionally annotated 98.27%of them,and identified repetitive sequences covering 49.08%of the *** found that *** is closely related to Prunus serrulata and Prunus yedoensis,having diverged from them∼41.8 million years ago.A comparative genomic analysis revealed that *** has 643 expanded and 1128 contracted gene ***,we found that *** is more resistant to Colletotrichum viniferum,Phytophthora capsici,and Pseudomonas syringae ***(Pst)DC3000 infections than cultivated Prunus *** also has considerably more nucleotide-binding site-type resistance gene analogs than ***,which explains its stronger disease *** cytochrome P450 and WRKY families of 263 and 61 proteins were divided into 42 and 8 subfamilies respectively in ***,81 MADS-box genes were identified in ***,accompanying expansions of the SVP and AGL15 subfamilies and loss of the TM3 *** assembly of a high-quality *** genome will be valuable for further research on cherries and molecular breeding.

关键词： Prunus resistance release

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Biomarkers of aging

Science china(Life sciences) 2023年第5期66卷 893-1066页

作者： Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang wang Qiaoran wang Siyuan wang Xuan wang Yaning wang Yuhan wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang yilin ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang yi Eve Sun Yu Sun Mei Tian Shusen wang Si wang Xia wang Xiaoning wang Yan-Jiang wang Yunfang wang Catherine CL Wong Andy Peng Xiang yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang tongbiao Zhao Yuzheng Zhao Dahai Zhu Weiguo Zou Gang Pei Guang-Hui Liu Aging Biomarker Consortium(ABC) Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China State Key Laboratory of Stem Cell and Reproductive biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China department of Dermatology Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Aging biology Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Clinic Center of Human Gene Research Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Clinical Research Center of Metabolic and Cardiovascular Disease Huazhong University of Science and TechnologyWuhan 430022China Hubei Key Laboratory of Metabolic Abnormalities and Vascular Aging Huazhong University of Science and TechnologyWuhan 430022China Stem Cell Translational Research Center Tongji HospitalTongji University School of MedicineShanghai 200065China department of Nuclear Medicine Daping HospitalThird Military Medical UniversityChongqing 400042China CAS Key Laboratory of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China shanghai Key Laboratory of Maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchShanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China The department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Ageing and Regenerative MedicineJ

Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological *** a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly *** immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this ***,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory *** fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.

关键词： aging senescence biomarker clock

In vivo antioxidant activity of rabbiteye blueberry(Vaccinium ashei cv.'Brightwell')anthocyanin extracts

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Journal of Zhejiang university-Science B(Biomedicine & Biotechnology) 2023年第7期24卷 602-616页

作者： Jing wang Xingyu ZHAO Jiawei ZHENG Daniela D.HERRERA-BALANDRANO Xiaoxiao ZHANG Wuyang HUANG Zhongquan SUI College of Chemical Engineering Nanjing Forestry UniversityNanjing 210037China Institute of Agro-Product Processing Jiangsu Academy of Agricultural SciencesNanjing 210014China school of food and Biological Engineering Jiangsu UniversityZhenjiang 212013China school of Life sciences Nantong UniversityNantong 226007China department of food Science&Engineering School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health *** purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from'Brightwell'rabbiteye blueberries in *** one week of adaptation,C57BL/6J healthy male mice were divided into different groups that were administered with 100,400,or 800 mg/kg blueberry anthocyanin extract(BAE),and sacrificed at different time points(0.1,0.5,1,2,4,8,or 12 h).The plasma,eyeball,intestine,liver,and adipose tissues were collected to compare their antioxidant activity,including total antioxidant capacity(T-AOC),superoxide dismutase(SOD)activity and glutathione-peroxidase(GSH-PX/GPX)content,and the oxidative stress marker malondialdehyde(MDA)*** results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in *** greater the concentration of BAE,the higher the T-AOC value,but the lower the MDA *** enzyme activity of SOD,the content of GSH-PX,and messenger RNA(mRNA)levels of Cu,Zn-SOD,Mn-SOD,and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant *** in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.

关键词： Blueberry anthocyanin In vivo antioxidant activity Superoxide dismutase(SOD) Glutathione-peroxidase(GSH-PX/GPX) Malondialdehyde(MDA)

表面增强拉曼光谱技术在动物源性食品兽药残留检测中的应用

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光谱学与光谱分析 2023年第6期43卷 1667-1675页

作者：李春颖王红义李永春李静陈高乐樊玉霞赤峰学院化学与生命科学学院内蒙古赤峰024000 上海交通大学农业与生物学院食品科学与工程系上海200240

动物源性食品是人类营养摄入必不可少的食品之一,兽药被广泛用于动物饲养和疾病防治,但兽药残留超标等问题对消费者的健康安全构成了严重威胁。为防止受污染的食品对消费者造成危害,研发快速有效的兽药残留分析方法非常必要。表面增强... 详细信息

动物源性食品是人类营养摄入必不可少的食品之一,兽药被广泛用于动物饲养和疾病防治,但兽药残留超标等问题对消费者的健康安全构成了严重威胁。为防止受污染的食品对消费者造成危害,研发快速有效的兽药残留分析方法非常必要。表面增强拉曼光谱法(SERS)作为一种痕量的检测方法,有望能够满足目前动物源性食品高效、快速、灵敏的检测需求。综述了SERS方法在动物源性食品兽药残留检测中的研究进展,包括肉类(猪肉、鸡肉、鸭肉、鱼肉)、乳和乳制品及蜂蜜中兽药残留的SERS分析研究。概述了SERS技术在肉类食品中主要兽药残留的检测应用进展。家禽肉中的兽药分析包括四环素类药物、磺胺类药物、恩诺沙星和激素类等药物;猪肉中的兽药主要分析了β-受体激动剂、氯霉素、左旋咪唑等药物;鱼肉中的兽药分析了染料类、磺胺类和氯霉素等药物。对乳和乳制品中的四环素类、氨基糖苷类、青霉素类、酰胺醇类药物的SERS检测进行了总结讨论。简述了SERS在蜂蜜中氯霉素类、四环素类等药物的分析。对SERS在动物源性食品的研究发展方向和应用前景进行了总结和展望。虽然SERS作为一种快速、超灵敏的检测方法,在分析复杂食品体系中的微量或痕量化合物方面,尤其是在食品中可能对健康造成危害的禁用和限用化学物质检测方面显示出了巨大的潜力,具有较好的发展前景,但依然面临极大的挑战。突破技术瓶颈,建立动物源性食品中兽药残留检测的SERS快速分析策略,开发出兽药残留的现场实时检测方案,将对食品安全检测监管具有重要意义。

关键词：表面增强拉曼光谱动物源性食品兽药残留食品安全

来自Yeosuana marina ***21内切型海藻酸裂解酶的异源表达及酶学表征

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生物技术通报 2022年第2期38卷 123-131页

作者：常晴束月蓉王文韬蒋昊延泉德钱政高雪纯吴金鸿张勇上海交通大学生命科学技术学院微生物代谢国家重点实验室上海200240 上海交通大学农业生物技术学院农业与生物学院食品科学与工程系上海200240

褐藻寡糖有着丰富的生物学功能,酶法制备功能性褐藻寡糖具有重要实践应用价值。为发掘高活性及稳定性的褐藻寡糖制备酶,对浅海热液嗜热菌Yeosuana marina ***21中的海藻酸裂解酶YMA-1的基因在大肠杆菌中进行表达、纯化及酶活鉴定。结果... 详细信息

褐藻寡糖有着丰富的生物学功能,酶法制备功能性褐藻寡糖具有重要实践应用价值。为发掘高活性及稳定性的褐藻寡糖制备酶,对浅海热液嗜热菌Yeosuana marina ***21中的海藻酸裂解酶YMA-1的基因在大肠杆菌中进行表达、纯化及酶活鉴定。结果发现YMA-1由306个氨基酸残基构成,是多糖裂解酶家族7(PL7)新成员;重组YMA-1酶的最适催化条件是55℃,pH 9.0,比活力1.3×10^(4) U/mg,Cu^(2+)可有效促进酶活;在37℃,pH 9.0条件下,该酶对海藻酸钠、聚甘露糖醛酸和聚古罗糖醛酸的比活力分别达到(5201.21±86.46)U/mg、(6399.73±253.12)U/mg和(3751.68±116.25)U/mg,酶解海藻酸钠终产物多为不饱和三糖和四糖,表现出内切双功能型海藻酸裂解酶活性。YMA-1酶作为PL7家族中较宽底物谱、高活性及稳定性的内切海藻酸裂解酶,在高效绿色生产功能性褐藻寡糖上有着潜在应用价值。

关键词：内切型海藻酸裂解酶重组表达酶学性质 Yeosuana marina sp.JLT21