检索结果-南通市图书馆

A polymyxin B loaded hypoxia-responsive liposome with improved biosafety for efficient eradication of bacterial biofilms

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第9期17卷 8325-8336页

作者： Fang Liu lingyun Zou Yongcheng Chen Zuolong Liu Yue Huang Qiao jin jian ji MOE key laboratory of macromolecule synthesis and functionalization of ministry of education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China State key laboratory of Transvascular Implantation Devices The Second Affiliated Hospital Zhejiang University School of MedicineHangzhou 310009China

Biofilm-associated bacterial infection brings serious threats to global public health owing to serious antibiotic *** is urgently needed to develop innovative strategies to combat biofilm-associated bacterial *** stand out as the last line of defense against Gram-negative ***,serious nephrotoxicity of polymyxins severely limits their clinical ***,a hypoxia-responsive liposome is designed as the nanocarrier of polymyxin B(PMB)to combat biofilms developed by Gram-negative bacteria.A metronidazole modified lipid(hypoxia-responsive lipid(HRLipid))is synthesized to fabricate hypoxia-responsive liposomes(HRLip).PMB loaded hypoxia-responsive liposomes(HRL-PMB)is then prepared to mitigate the nephrotoxicity of PMB while preserving its excellent bactericidal ***-PMB shows very low hemolysis and cytotoxicity due to liposomal encapsulation of *** can be readily released from HRL-PMB in response to hypoxic biofilm microenvironment,exerting its bactericidal activity to realize biofilm *** excellent in vivo antibiofilm ability of HRL-PMB is confirmed by a Pseudomonas aeruginosa infected zebrafish model and a *** pneumonia infection ***,HRL-PMB can greatly reduce the nephrotoxicity of PMB after intravenous *** hypoxia-sensitive liposomes held great promise to improve the biosafety of highly toxic antibiotics while preserving their intrinsic bactericidal ability,which may provide an innovative strategy for combating biofilm-associated infections.

关键词： bacterial biofilm hypoxia liposome polymyxin B nephrotoxicity

Hierarchical Capillary Coating to Biofunctionlize Drug-Eluting Stent for Improving Endothelium Regeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research 2020年第1期2020卷 81-94页

作者： jing wang Yunfan Xue Jun Liu Mi Hu He Zhang kefeng ren Yunbing wang jian ji MOE key laboratory of macromolecule synthesis and functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China

The drug-eluting stent(DES)has become one of the most successful and important medical devices for coronary heart disease,but yet suffers from insufficient endothelial cell(EC)growth and intima repair,eventually leading to treatment *** biomacromolecules such as vascular endothelial growth factor(VEGF)would be promising to promote the intima regeneration,combining hydrophilic and vulnerable biomacromolecules with hydrophobic drugs as well as preserving the bioactivity after harsh treatments pose a huge ***,we report on a design of hierarchical capillary coating,which composes a base solid region and a top microporous region for incorporating rapamycin and VEGF,*** top spongy region can guarantee the efficient,safe,and controllable loading of VEGF up to 1μg/cm^(2) in 1 minute,providing a distinctive real-time loading capacity for saving the *** on this,we demonstrate that our rapamycin-VEGF hierarchical coating impressively promoted the competitive growth of endothelial cells over smooth muscle cells(ratio of EC/SMC~25)while relieving the adverse impact of rapamycin to *** further conducted the real-time loading of VEGF on stents and demonstrate that the hierarchical combination of rapamycin and VEGF showed remarkable endothelium regeneration while maintaining a very low level of in-stent *** work paves an avenue for the combination of both hydrophobic and hydrophilic functional molecules,which should benefit the next generation of DES and may extend applications to diversified combination medical devices.

关键词： Regeneration eventually guarantee

The relief of hypoxic microenvironment using an O_(2) self-sufficient fluorinated nanoplatform for enhanced photodynamic eradication of bacterial biofilms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2022年第2期15卷 1636-1644页

作者： lingyun Zou Dengfeng Hu Fanjin wang Qiao jin jian ji MOE key laboratory of macromolecule synthesis and functionalization of ministry of education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China

Hypoxia,an important characteristic of bacterial biofilms,can hinder the generation of reactive oxygen species(ROS)in photodynamic therapy(PDT),leading to reduced therapeutic efficacy of *** order to address this issue,fluorinated liposome was fabricated as an oxygen-sufficient nanoplatform for enhanced photodynamic eradication of bacterial *** liposomes(denoted as Lip-Ce6-PFH@O_(2))were prepared by co-encapsulation of O_(2) carrier perfluorohexane(PFH)and photosensitizer chlorin e6(Ce_(6)).Lip-Ce6-PFH@O_(2) could achieve efficient biofilm penetration due to the positively charged *** hypoxic microenvironment of biofilms would then be relieved,leading to the generation of more ROS under laser ***,the bactericidal capability of PDT could be significantly improved because of the co-delivered O_(2) carrier ***-Ce6-PFH@O_(2) exhibited much better antibiofilm ability than that of Lip-Ce6 both in vitro and in ***,Lip-Ce6-PFH@O_(2) also effectively alleviated inflammation symptoms and accelerated wound healing in the mice *** general,this study provides a new paradigm to enhance the therapeutic efficacy of PDT for efficient biofilm eradication.

关键词： biofilm hypoxia perfluorohexane liposome photodynamic therapy

Ratiometrically Designed Nanocarrier to Impact Major Cancer Pathways for Effective Pancreatic Cancer Treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2020年第6期36卷 1143-1144页

作者： ji jian key laboratory of macromolecule synthesis and functionalization of ministry of education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027P.R.China

To achieve secured in vivo drug synergy in stroma-rich pancreatic cancer(PDAC)is not an easy *** and Liu et *** the rational development of a ratiometrically designed nanocarrier to co-package a CDK4/6 cel cycle inhibitor palbociclib(PAL)plus an autophagy inhibitor hydroxychloroquine(HCQ)for efficient PDAC treatment The lead formulation was obtained using a computer software aided in vitro screening,followed by a remote drug co-import into a lipid-coated mesoporous silica *** ratiometric nanocarrier led to the synchronized phar-macokinetic(PK)profile and effective intratumoral buildup of the drug pair post intravenous injection,which otherwise exhibited distinctly different biodistribution *** treatment using PAL/HCQ co-delivery nanoparticle:yielded the most effective shrinkage of PDAC in subcutaneous and orthotopic PANCI mouse *** mechanistic investigation also demonstrated the activation of anti-apoptosis pathway after repetitive nanoparticle injection in mice which promoted the authors to introduce a small molecule anti-apoptosis inhibitor to further improve the performance of their already potent nanoparticle in the PDAC mouse *** work has been published online in Nature Communi cations on August 25,2020.

关键词： injection otherwise treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物医用高通量研究中的微液滴阵列

化学进展 2021年第4期33卷 543-554页

作者：陈怡峰王聪任科峰计剑浙江大学高分子科学与工程学系教育部高分子合成与功能构造重点实验室杭州310027

为快速、高效处理大量实验及其数据,进一步加快材料研发过程,高通量筛选成为了一种越来越重要的实验手段,被广泛应用于众多领域,以提升实验效率。而高通量实验平台是开展高通量实验的基础条件。现有高通量实验平台,如微孔板,在处理珍贵... 详细信息

为快速、高效处理大量实验及其数据,进一步加快材料研发过程,高通量筛选成为了一种越来越重要的实验手段,被广泛应用于众多领域,以提升实验效率。而高通量实验平台是开展高通量实验的基础条件。现有高通量实验平台,如微孔板,在处理珍贵样品和试剂时,仍存在消耗量较大、实验通量较低等问题,仍有待进一步优化。而微液滴阵列作为一种新兴的微型化和集成化高通量平台,具有试剂及样品消耗少、反应时间短、高度集成化、可操作性强等优势,在生物医用领域得到了广泛的研究和应用。本文总结归纳了微液滴阵列的制备方法,将其划分为表面化学驱动和物理形貌辅助两大类,并对不同制备方法的优缺点进行了简要的分析;随后从2D细胞筛选、3D细胞培养、单细胞分析及全机体筛选四个方向对其在生物医用高通量研究中的应用进行了简要介绍,最后总结分析了微液滴阵列在应用过程中存在的问题和未来的发展方向。

关键词：微液滴阵列制备方法生物医用高通量

The Escherichia coli O157:H7 DNA detection on a gold nanoparticle-enhanced piezoelectric biosensor

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2008年第8期53卷 1175-1184页

作者： wang Lijiang WEI QingShan WU ChunSheng HU ZhaoYing ji jian wang Ping Biosensor National Special laboratory and key laboratory of Biomedical Engineering of ministry of education Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China key laboratory of macromolecule synthesis and functionalization of ministry of education Zhejiang University Hangzhou 310027 China

This paper presents development of a quartz crystal microbalance (QCM) biosensor for real-time de- tection of E. coli O157:H7 DNA based on nanogold particles amplification. Many inner Au nanoparticles were immobilized onto the thioled surface of the Au electrode, then more specific thiolated sin- gle-stranded DNA (ssDNA) probes could be fixed through Au-SH bonding. The hybridization was in- duced by exposing the ssDNA probe to the complementary target DNA of E. coli O157:H7 gene eaeA, then resulted in a mass change and corresponding frequency shifts ( △f ) of the QCM. The outer avidin-coated Au nanoparticles could combine with the target DNA to increase the mass. The electro- chemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were adopted to manifest and character each step. The target DNA corresponding to 2.0×103 colony forming unit (CFU)/mL E. coli O157:H7 cells can be detected by this biosensor, so it is practical to develop a sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analy- sis. The piezoelectric biosensing system has potential for further applications, such as food safety and environment monitoring, and this approach lays the groundwork for incorporating the method into an integrated system for in-field bacteria detection.

关键词：压电生物受感器黄金纳米颗粒 DNA 大肠埃希氏菌

An ROS-Responsive Antioxidative Macromolecular Prodrug of Caffeate for Uveitis Treatment

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2022年第9期40卷 1101-1109页

作者： Yu-Tong Li Si-Ting Sheng Bo Yu Fan jia Kai wang Hai-jie Han Qiao jin You-Xiang wang jian ji MOE key laboratory of macromolecule synthesis and functionalization of ministry of education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Eye Center the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China

Uveitis is a sophisticated syndrome showing a high relevance with reactive oxygen species(ROS).Herein,an ROS-responsive PEGylated polypeptide based macromolecular prodrug of herbaceous antioxidant ethyl caffeate(EC)is designed via phenylboronic esters with improved solubility for the alleviation of *** antioxidative 4-hydroxybenzyl alcohol(HBA)and EC can be released from the macromolecular EC prodrug under the stimulation of ROS,which can effectively protect cells against oxidative stress-induced injury in an ROS-depletion *** antioxidative and protective effects of the macromolecular EC prodrug in vivo are further verified in a uveitis mouse ***,this work not only provides a handy method to synthesize a phenylboronic ester-bearing EC prodrug which is highly sensitive to pathological ROS,but also depicts a promising future to apply macromolecular antioxidative prodrugs in the treatment of uveitis as well as other ROS-related diseases.

关键词： Antioxidative therapy Macromolecular prodrug Uveitis Ethyl caffeate(EC) Phenylboronic acid esters

Norvancomycin-capped silver nanoparticles: synthesis and antibacterial activities against E. coli

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2007年第3期50卷 418-424页

作者： WEI QingShan ji jian FU jinHong SHEN jiaCong Department of Polymer Science and Engineering Key Laboratory of Macromolecule Synthesis and Functionalization(Ministry of Education)Zhejiang UniversityHangzhou 310027China

The synthesis of norvancomycin (NVan)-capped silver nanoparticles (Ag@NVan) and their notable in vitro antibacterial activities against E. coli, a Gram-negative bacterial strain (GNB), are reported here. Mercaptoacetic acid-stabilized spherical silver nanoparticles with a diameter of 16±4 nm are prepared by a simple chemical reaction. The formation process of the silver nanoparticles is investigated by UV-visible (UV-vis) spectroscopy and transmission electron microscopy (TEM). NVan is then grafted to the terminal carboxyl of the mercaptoacetic acid in the presence of N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC). The TEM images of single bacteria treated with Ag@NVan show that plenty of Ag@NVan aggregate in the cell wall of E. coli. A possible antibacterial mechanism is proposed that silver nanoparticles may help destroy the stability of the outer membrane of E. coli, which makes NVan easier to bind to the nether part of the peptidoglycan structure. The antibacterial activities of silver nanoparticles on their own, together with the rigid polyvalent interaction between Ag@NVan and cell wall, enables Ag@NVan to be an effective inhibitor of GNB. This kind of bionanocomposites might be used as novel bactericidal materials and we also provide an effective synthesis method for preparing functional bioconjugated nanoparticles here.

关键词： silver nanoparticle norvancomycin Gram-negative bacterial strain bioconjugate

Emerging pro-drug and nano-drug strategies for gemcitabine-based cancer therapy

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2022年第1期17卷 35-52页

作者： Haijie Han Su Li Yueyang Zhong Yue Huang Kai wang Qiao jin jian ji Ke Yao Eye Center the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Zhejiang Provincial key Lab of Ophthalmology the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China MOE key laboratory of macromolecule synthesis and functionalization of ministry of education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China

Gemcitabine has been extensively applied in treating various solid tumors. Nonetheless,the clinical performance of gemcitabine is severely restricted by its unsatisfactory pharmacokinetic parameters and easy deactivation mainly because of its rapid deamination, deficiencies in deoxycytidine kinase (DCK), and alterations in nucleoside transporter. On this account, repeated injections with a high concentration of gemcitabine are adopted, leading to severe systemic toxicity to healthy cells. Accordingly, it is highly crucial to fabricate efficient gemcitabine delivery systems to obtain improved therapeutic efficacy of gemcitabine. A large number of gemcitabine pro-drugs were synthesized by chemical modification of gemcitabine to improve its biostability and bioavailability. Besides,gemcitabine-loaded nano-drugs were prepared to improve the delivery efficiency. In this review article, we introduced different strategies for improving the therapeutic performance of gemcitabine by the fabrication of pro-drugs and nano-drugs. We hope this review will provide new insight into the rational design of gemcitabine-based delivery strategies for enhanced cancer therapy.

关键词： Gemcitabine Pro-drug Nano-drug Cancer therapy